{"id":14248,"date":"2017-09-26T12:47:46","date_gmt":"2017-09-26T04:47:46","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14248"},"modified":"2022-07-21T10:32:58","modified_gmt":"2022-07-21T02:32:58","slug":"caspase-8-%e5%91%88%e8%89%b2%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-%e8%b2%a8%e8%99%9fk113","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=14248","title":{"rendered":"Caspase-8 \u5448\u8272\u5206\u6790\u5957\u7d44 | Caspase-8 Colorimetric Assay Kit\u8ca8\u865fK113"},"content":{"rendered":"

Caspase-8 <\/strong>\u5448\u8272\u5206\u6790\u5957\u7d44<\/strong> | \u8ca8\u865f<\/strong>K113<\/strong><\/a><\/span><\/p>\n

Caspase<\/strong>\u7cfb\u5217\u7522\u54c1\u4ecb\u7d39<\/strong>\uff1a<\/strong><\/p>\n

\u7576\u7d30\u80de\u63a5\u6536\u51cb\u4ea1\u8a0a\u865f\u5f8c\uff0c\u5c07\u4e0d\u6d3b\u5316\u614b\u7684pro-caspase\u8f49\u8b8a\u70ba\u6d3b\u5316\u614bcaspase\uff0c\u5f15\u767ccaspase cascade\u9020\u6210\u7d30\u80de\u51cb\u4ea1\u3002\u4e3b\u8981\u7d93\u7531Caspases 8\u30019\u300112\u8abf\u63a7\u7d30\u80de\u51cb\u4ea1\u7684\u5916\u5728\u8def\u5f91(extrinsic pathway)\u3001\u5167\u5728(intrinsic pathway)\u3001\u4ee5\u53caER stress\uff0c\u6700\u5f8c\u9020\u6210Caspases 3\u7684\u6d3b\u5316\u3002Biovision\u63d0\u4f9b\u5b8c\u6574Caspase\u76f8\u95dc\u5206\u6790\u5957\u7d44\u3001inhibitor\u3001substrate\u7b49\u5404\u985e\u5546\u54c1\u3002Caspase \u5206\u6790\u5957\u7d44\uff0fSubstrate: \u5f9eCaspase1-13\u3001Pan Caspase\u7686\u80fd\u5206\u6790\u3002\u5206\u6790\u5957\u7d44\u90e8\u5206\u6709\u5316\u5b78\u5448\u8272\u53cd\u61c9\u53ca\u87a2\u5149\u53ef\u9078\u64c7\uff1bSubstrate\u90e8\u5206\u6709\u5316\u5b78\u5448\u8272\u7269\u8ceapNA\uff08\u03bb= 400 nm\uff09\u8207\u87a2\u5149\u5448\u8272\u7269\u8ceaAFC\uff08Ex\/Em\uff1d400\/505 nm\uff09\u5169\u7a2e\u53ef\u61c9\u7528\u3002Caspase Inhibitors: Caspase \u6291\u5236\u5291\u4e3b\u8981\u7d50\u5408Caspase\u6d3b\u5316\u4f4d\uff0c\u9032\u800c\u9054\u5230\u8a0a\u606f\u6291\u5236\u6548\u679c\u3002fluoromethylketone\uff08FMK\uff09\u985e\u7684\u6291\u5236\u5291\uff0c\u70ba\u4e3b\u8981\u7814\u7a76\u6297\u7d30\u80de\u51cb\u4ea1\u7684\u5de5\u5177\u4e4b\u4e00\u3002\u65b0\u4e00\u4ee3Caspase\u6291\u5236\u5291Q-(XXXX)-OPh\uff0c\u56e0\u5176\u7d30\u80de\u6bd2\u6027\u8f03\u4f4e\uff0c\u4e14\u7a69\u5b9a\u6027\u8f03FMK\u985e\u6291\u5236\u5291\u9ad8\uff0c\u8f03\u9069\u5408\u5728in vivo\u5be6\u9a57\u4e2d\u4f7f\u7528\u3002<\/p>\n

Caspase-8 <\/strong>\u5448\u8272\u5206\u6790\u5957\u7d44<\/strong> \u539f\u7406\u4ecb\u7d39<\/strong>:<\/strong> ICE\u5bb6\u65cf\u86cb\u767d\u9176\/\u534a\u80f1\u5929\u51ac\u9176\u7684\u6fc0\u6d3b\u555f\u52d5\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u51cb\u4ea1\u3002 FLICE \/ Caspase-8\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u65b9\u4fbf\u7684\u65b9\u6cd5\u4f86\u6e2c\u5b9a\u8b58\u5225\u5e8f\u5217IETD\u7684\u534a\u80f1\u5929\u51ac\u9176\u7684\u6d3b\u6027\u3002 \u8a72\u6e2c\u5b9a\u6cd5\u57fa\u65bc\u5f9e\u6a19\u8a18\u7684\u5e95\u7269IETD-pNA\u5207\u5272\u5f8c\u7684\u9aee\u8272\u5718\u5c0d\u785d\u57fa\u82ef\u80fa\uff08pNA\uff09\u7684\u5206\u5149\u5149\u5ea6\u6aa2\u6e2c\u3002 \u53ef\u4ee5\u4f7f\u7528\u5206\u5149\u5149\u5ea6\u8a08\u6216\u5fae\u91cf\u6ef4\u5b9a\u677f\u8b80\u6578\u5668\u5728400\u6216405nm\u4e0b\u91cf\u5316pNA\u5149\u767c\u5c04\u3002<\/p>\n\n\n\n\n\n\n\n
Detection Method<\/strong><\/td>\nAbsorbance (400 or 405 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nDetect early\/middle stages of apoptosis; differentiate apoptosis from necrosis.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple one-step procedure; takes 1-2 hours
\n\u2022 Fast and convenient
\n\u2022 Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Cell Lysis Buffer
\n\u2022 2X Reaction Buffer
\n\u2022 IETD-pNA (4 mM)
\n\u2022 DTT (1 M)
\n\u2022 Dilution Buffer<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

\n
Larissa M. Ruthenium(II) complexes with 6-methyl-2-thiouracil selectively reduce cell proliferation, cause DNA double-strand break and trigger caspase-mediated apoptosis through JNK\/p38 pathways in human acute promyelocytic leukemia cells.\u00a0Sci Rep, Aug 2019; \u00a031391500.<\/a><\/div>\n<\/div>\n
\n
Emel AKSOY, Schmallenberg virus induces apoptosis in Vero cell line via extrinsic and intrinsic pathways in a time and dose dependent manner.\u00a0J Vet Med Sci, Dec 2018;\u00a0\u00a030541984.<\/a><\/div>\n<\/div>\n
\n
Wang, Jie et al. (2016) Sulfated polysaccharide\u2011protein complex sensitizes doxorubicin\u2011induced apoptosis of breast cancer cells in vitro and in vivo,\u00a0Exp Ther Med. 2016 Oct;12(4):2169-2176.<\/a><\/div>\n<\/div>\n
\n
Griffiths et al., Bit-1 is an essential regulator of myogenic differentiation.\u00a0J. Cell Sci., May 2015; 128: 1707 – 1717.<\/a><\/div>\n<\/div>\n
\n
Palchetti et al., Transfected Poly(I:C) Activates Different dsRNA Receptors, Leading to Apoptosis or Immunoadjuvant Response in Androgen-independent Prostate Cancer Cells.\u00a0J. Biol. Chem.,\u00a0Feb 2015; 290: 5470 – 5483.<\/a><\/div>\n
<\/div>\n<\/div>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u5546\u54c1\u578b\u865f<\/td>\n\u7522\u54c1\u540d\u7a31<\/td>\n\u5305\u88dd\u898f\u683c<\/td>\n<\/tr>\n
Caspase<\/strong>\u87a2\u5149\u5206\u6790\u5957\u7d44<\/strong><\/td>\n<\/tr>\n
K110-100<\/td>\nCaspase-1 Fluorometric Assay<\/td>\n100 assays<\/td>\n<\/tr>\n
K116-100<\/td>\nCaspase-2 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K105-100<\/td>\nCaspase-3 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K126-100<\/td>\nCaspase-4 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K122-100<\/td>\nCaspase-5 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K114-100<\/td>\nCaspase-6 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K112-100<\/td>\nCaspase-8 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K118-100<\/td>\nCaspase-9 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K124-100<\/td>\nCaspase-10 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K139-100<\/td>\nCaspase-12 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
Caspase<\/strong>\u5448\u8272\u5206\u6790\u5957\u7d44<\/strong><\/td>\n<\/tr>\n
K111-100<\/td>\nCaspase-1 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K117-100<\/td>\nCaspase-2 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K106-100<\/td>\nCaspase-3 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K127-100<\/td>\nCaspase-4 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K123-100<\/td>\nCaspase-5 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K115-100<\/td>\nCaspase-6 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K113-100<\/td>\nCaspase-8 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K119-100<\/td>\nCaspase-9 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K125-100<\/td>\nCaspase-10 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
Caspase FMK<\/strong>\u985e\u6291\u5236\u5291<\/strong><\/td>\n<\/tr>\n
1012-100
\n1012-20C<\/td>\n		Caspase-1 Inhibitor, Z-YVAD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1073-100
\n1073-20C<\/td>\n		Caspase-2 Inhibitor, Z-VDVAD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1009-100
\n1009-20C<\/td>\n		Caspase-3 Inhibitor, Z-DEVD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1108-100
\n1108-20C<\/td>\n		Caspase-4 Inhibitor, Z-LEVD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1100-100
\n1100-20C<\/td>\n		Caspase-5 Inhibitor, Z-WEHD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1011-100
\n1011-20C<\/td>\n		Caspase-6 Inhibitor, Z-VEID-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1064-100
\n1064-20C<\/td>\n		Caspase-8 Inhibitor, Z-IETD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1074-100
\n1074-20C<\/td>\n		Caspase-9 Inhibitor, Z-LEHD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1112-100
\n1112-20C<\/td>\n		Caspase-10 Inhibitor, Z-AEVD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1079-100
\n1079-20C<\/td>\n		Caspase-12 Inhibitor, Z-ATAD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1115-100
\n1115-20C<\/td>\n		Caspase-13 Inhibitor, Z-LEE-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1010-100
\n1010-20C<\/td>\n		Caspase-Family Inhibitor, Z-VAD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1120-20C<\/td>\nCaspase Inhibitor Boc-D-FMK<\/td>\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Caspase-8 \u5448\u8272\u5206\u6790\u5957\u7d44 | \u8ca8\u865fK113 Caspase\u7cfb\u5217\u7522\u54c1\u4ecb\u7d39\uff1a \u7576\u7d30\u80de\u63a5\u6536\u51cb\u4ea1\u8a0a\u865f\u5f8c\uff0c\u5c07\u4e0d\u6d3b […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[128],"tags":[3174],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14248"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14248"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14248\/revisions"}],"predecessor-version":[{"id":28178,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14248\/revisions\/28178"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14248"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14248"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14248"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}