{"id":14231,"date":"2017-09-24T13:27:04","date_gmt":"2017-09-24T05:27:04","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=14231"},"modified":"2025-05-08T13:14:01","modified_gmt":"2025-05-08T05:14:01","slug":"%ce%b1-%e9%85%ae%e6%88%8a%e4%ba%8c%e9%85%b8%e5%91%88%e8%89%b2%e8%9e%a2%e5%85%89%e6%b8%ac%e5%ae%9a%e6%aa%a2%e6%b8%ac-alpha-ketoglutarate-colorimetricfluorometric-assay-kit%e8%b2%a8%e8%99%9fk677-1","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=14231","title":{"rendered":"\u03b1-\u916e\u620a\u4e8c\u9178\u5448\u8272\/\u87a2\u5149\u6e2c\u5b9a\u6aa2\u6e2c | Alpha-Ketoglutarate Colorimetric\/Fluorometric Assay Kit\u8ca8\u865fK677 -100 (ab83431)"},"content":{"rendered":"

\u03b1<\/strong>–<\/strong>\u916e\u620a\u4e8c\u9178<\/strong>\u5448<\/strong>\u8272<\/strong>\/<\/strong>\u87a2<\/strong>\u5149\u6e2c\u5b9a\u8a66\u5291\u76d2<\/strong>–<\/strong>\u4e59\u919b\u9178\u5faa\u74b0<\/strong>\u5206\u6790\u5546\u54c1<\/strong> | K677 -100 (ab83431)<\/strong><\/a><\/span><\/p>\n

Alpha-Ketoglutarate Colorimetric\/Fluorometric Assay Kit<\/p>\n

\u4e59\u919b\u9178\u5faa\u74b0\u662f\u4e09\u7fa7\u9178\u5faa\u74b0\u7684\u8b8a\u5316\uff0c\u662f\u5728\u690d\u7269\uff0c\u7d30\u83cc\uff0c\u539f\u751f\u751f\u7269\u548c\u771f\u83cc\u4e2d\u767c\u751f\u7684\u5408\u6210\u4ee3\u8b1d\u9014\u5f91\u3002 \u7576\u4e0d\u80fd\u7372\u5f97\u8af8\u5982\u8461\u8404\u7cd6\u7b49\u8907\u96dc\u4f86\u6e90\u6642\uff0c\u8a72\u5faa\u74b0\u5141\u8a31\u7d30\u80de\u5229\u7528\u7c21\u55ae\u7684\u78b3\u5316\u5408\u7269\u4f5c\u70ba\u78b3\u6e90\u3002 BioVision\u70ba\u6d89\u53ca\u4e59\u919b\u9178\u9014\u5f91\u7684\u4e0d\u540c\u4ee3\u8b1d\u7269\u63d0\u4f9b\u6613\u65bc\u4f7f\u7528\u548c\u9ad8\u901a\u91cf\u7684\u9069\u61c9\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u3002 \u9019\u4e9b\u6e2c\u5b9a\u8a66\u5291\u76d2\u662f\u9ad8\u5ea6\u654f\u611f\u7684\u4e26\u4e14\u8207\u5ee3\u6cdb\u7684\u6a23\u54c1\u985e\u578b\u517c\u5bb9<\/p>\n

<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>: <\/strong>\u03b1<\/strong>-Ketoglutarate (\u03b1-KG) <\/strong>\u03b1-\u916e\u620a\u4e8c\u9178\u5448\u8272\/\u87a2\u5149\u6e2c\u5b9a\u8a66\u5291\u76d2; \u03b1-\u916e\u620a\u4e8c\u9178\uff08\u03b1-KG\uff09\u662fKrebs cycle\u514b\u96f7\u5e03\u65af\u5faa\u74b0\u4e2d\u7684\u95dc\u9375\u4e2d\u9593\u9ad4\uff0c\u4f86\u81ea\u7570\u6ab8\u6aac\u9178\u916f\u548c\u7425\u73c0\u91afCoA\u4e4b\u524d\u3002 \u901a\u904e\u8c37\u6c28\u9178\u8f49\u6c28\u9176\u6216\u8c37\u6c28\u9178\u812b\u6c2b\u9176\u7684\u4f5c\u7528\uff0c\u901a\u904e\u5408\u6210\u03b1-KG\u4f86\u88dc\u5145\u9031\u671f\u3002 \u03b1-KG\u662f\u91cd\u8981\u7684\u6c2e\u8f49\u904b\u86cb\u767d\u3002 \u4f5c\u70ba\u91cd\u8981\u7684\u4e2d\u9593\u9ad4\uff0c\u662f\u65b0\u751f\u5152\u6e2c\u91cf\u7684\u6709\u6a5f\u9178\u4e4b\u4e00\uff0c\u4f5c\u70ba\u5148\u5929\u6027\u4ee3\u8b1d\u932f\u8aa4\u7684\u6307\u6a19\u3002 BioVision\u7684\u03b1-\u916e\u620a\u4e8c\u9178\u5206\u6790\u8a66\u5291\u76d2\u70ba\u5404\u7a2e\u6a23\u54c1\u4e2d\u7684\u03b1-KG\u91cf\u5316\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\uff0c\u9748\u654f\u548c\u5feb\u901f\u7684\u65b9\u6cd5\u3002 \u5728\u6e2c\u5b9a\u4e2d\uff0c\u03b1-\u916e\u620a\u4e8c\u9178\u8f49\u5316\u70ba\u7522\u751f\u4e19\u916e\u9178\u9e7d\uff0c\u5176\u7528\u65bc\u5c07\u8fd1\u4f3c\u7121\u8272\u7684\u63a2\u91dd\u8f49\u5316\u6210\u5169\u7a2e\u984f\u8272\uff08\u03bbmax= 570nm\uff09\u548c\u87a2\u5149\uff08Ex \/ Em = 535 \/ 5887nm\uff09\u3002 \u03b1-\u916e\u620a\u4e8c\u9178\u6e2c\u5b9a\u8a66\u5291\u76d2\u53ef\u7528\u65bc\u6aa2\u6e2c0.01\u301c10nmol\u7bc4\u570d\u5167\u7684\u03b1-\u916e\u620a\u4e8c\u9178\u3002<\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/th>\nK677-100 (ab83431)<\/td>\n<\/tr>\n
Size<\/th>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/th>\nAbsorbance (570 nm) or Fluorescence (Ex\/Em 535\/587 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/th>\nMammalian<\/td>\n<\/tr>\n
Applications<\/th>\nThe \u03b1-Ketoglutarate Assay Kit is useful for detecting \u03b1-ketoglutarate in the range of 0.01 to 10 nmoles.<\/td>\n<\/tr>\n
Features & Benefits<\/th>\n\u2022 Simple procedure; takes ~ 30 minutes to complete the assay
\n\u2022 Fast and convenient
\n\u2022 Highly sensitive assays for measuring alpha-Ketaglutarate levels in biological samples<\/td>\n<\/tr>\n
Kit Components<\/th>\n\u2022 \u03b1-KG Assay Buffer
\n\u2022 \u03b1-KG OxiRed Probe (in DMSO)
\n\u2022 \u03b1-KG Converting Enzyme
\n\u2022 \u03b1-KG Development Enzyme Mix
\n\u2022 \u03b1-KG Standard (10 \u00b5mol)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

B<\/strong>ioVision<\/strong>\u4ee3\u8b1d\u5206\u6790<\/strong>\u7522\u54c1\u4ecb\u7d39<\/strong><\/p>\n

Metabolism<\/strong>\u4ee3\u8b1d\uff1a<\/strong>\u4ee3\u8b1d\u662f\u6240\u6709vital\u751f\u547d\u5f62\u5f0f\u7684\u91cd\u8981\u904e\u7a0b\u3002\u5b83\u88ab\u5ee3\u6cdb\u5730\u5b9a\u7fa9\u70ba\u7dad\u6301\u7d30\u80de\u7684\u751f\u547d\u72c0\u614b\uff0c\u5f9e\u800c\u6d89\u53ca\u751f\u7269\u9ad4\u7684\u6240\u6709\u751f\u7269\u5316\u5b78\u904e\u7a0b\u7684\u7e3d\u548c\u3002\u4eac\u90fd\u767e\u79d1\u5168\u66f8\u57fa\u56e0\u548c\u57fa\u56e0\u7d44\u4e2d\u8a3b\u660e\u8d85\u904e87,000\u500b\u53cd\u61c9\u548c16,000\u500b\u4ee3\u8b1d\u7269\u3002\u5927\u91cf\u71df\u990a\u7d20\u5982\u98df\u7269\u4e2d\u7684carbohydrates\u78b3\u6c34\u5316\u5408\u7269\u3001proteins\u86cb\u767d\u8cea\u548cfats\u8102\u80aa\u8f49\u5316\u70ba\u80fd\u91cf\u662f\u4ee3\u8b1d\u904e\u7a0b\u7684\u7d44\u6210\u90e8\u5206\u3002\u4ee3\u8b1d\u904e\u7a0b\u53ef\u4ee5\u5206\u70ba\u5169\u500b\u4e3b\u8981\u985e\u5225\uff1a\u5408\u6210\u4ee3\u8b1d\u904e\u7a0b\uff0c\u5176\u6d88\u8017\u80fd\u91cf\u4ee5\u5408\u6210\u7c21\u55ae\u5206\u5b50\u6216\u5c07\u5176\u805a\u5408\u6210\u66f4\u8907\u96dc\u7684\u5927\u5206\u5b50\uff0c\u4f8b\u5982\u3002 DNA\uff0cRNA\u548c\u86cb\u767d\u8cea\u4ee5\u53ca\u964d\u89e3\u5206\u5b50\u91cb\u653e\u80fd\u91cf\u7684\u5206\u89e3\u4ee3\u8b1d\u904e\u7a0b\u3002\u6b64\u5916\uff0c\u4ee3\u8b1d\u904e\u7a0b\u4e5f\u6709\u52a9\u65bc\u53bb\u9664\u6709\u6bd2\u7269\u8cea\u3002<\/p>\n

\u4ee3\u8b1d\u4e4b\u529f\u80fd\uff1a<\/strong>\u4ee3\u8b1d\u5728physiology\u751f\u7406\uff0ccell biology\u7d30\u80de\u751f\u7269\u5b78\u548cmedicine.\u91ab\u5b78\u7684\u4e0d\u540c\u9818\u57df\u626e\u6f14\u95dc\u9375\u4f5c\u7528\u3002\u901a\u904e\u8a8d\u8b58\u5230\u8a31\u591a\u5e38\u898b\u7684\u4eba\u985e\u75be\u75c5\u5982cancer\u764c\u75c7\uff0cdiabetes\u7cd6\u5c3f\u75c5\uff0cobesity\u80a5\u80d6\u75c7\uff0chypoglycemia\u4f4e\u8840\u7cd6\u75c7\uff0chypolipidemia\u4f4e\u8840\u8102\u75c7\uff0cphenylketonuria\u82ef\u4e19\u916e\u5c3f\u75c7\uff0cneurodegeneration\u795e\u7d93\u8b8a\u6027\u7b49\u90fd\u662f\u7531\u65bc\u4ee3\u8b1d\u9014\u5f91\u7684\u64fe\u52d5\u800c\u5f15\u8d77\u7684\u3002\u56e0\u6b64\uff0c\u4e86\u89e3\u4ee3\u8b1d\u8b8a\u7570\u548c\u53ef\u80fd\u7684\u7570\u5e38\u503c\u662f\u75be\u75c5\u5c0e\u5411\u7814\u7a76\u7684\u95dc\u9375\u65b9\u9762\u3002BioVision\u63d0\u4f9b\u5f9e\u4e00\u7cfb\u5217carbohydrates,\u78b3\u6c34\u5316\u5408\u7269\uff0clipids\u8102\u8cea\uff0camino acids\u6c28\u57fa\u9178\u548cproteins\u86cb\u767d\u8cea\uff0cinorganic ions\u7121\u6a5f\u96e2\u5b50\uff0cserum components\u8840\u6e05\u6210\u5206\uff0ccoenzymes & cofactors\u8f14\u9176\u548c\u8f14\u56e0\u5b50\u5230\u6c27\u5316oxidative stress\uff0c\u7cd6\u9175\u89e3\u9014\u5f91\u548c\u4e2d\u9593\u4ee3\u8b1d\u7b49\u6700\u5ee3\u6cdb\u7684\u4ee3\u8b1d\u7522\u7269\u7d44\u5408\u3002\u6b64\u5916\uff0cBioVision\u9084\u70ba\u6709\u8208\u8da3\u6aa2\u6e2c\u4ee3\u8b1d\u7522\u7269\u7684submicromolar\u4e9e\u5fae\u6469\u723e\u6fc3\u5ea6\u7684\u7814\u7a76\u4eba\u54e1\u63d0\u4f9b\u5404\u7a2ePicoProbe\u2122\u6e2c\u5b9a\u8a66\u5291\u76d2\u3002 PicoProbe\u2122\u662fBioVision\u7684\u5546\u6a19\u3002\u9019\u4e9bPicoProbe\u2122\u6e2c\u5b9a\u8a66\u5291\u76d2\u662f\u5e02\u5834\u4e0a\u6700\u654f\u611f\u7684\u8a66\u5291\u76d2\uff0c\u53ef\u6839\u64da\u6aa2\u6e2c\u985e\u578b\u6aa2\u6e2c\u9ad8\u905420 pmol\u6216\u751a\u81f3\u66f4\u4f4e\u7684\u4ee3\u8b1d\u7269\u3002<\/p>\n

\u4ee3\u8b1d\u5206\u6790\u5546\u54c1<\/strong>\u7279\u9ede\u8207\u512a\u52e2<\/strong><\/p>\n

    \n
  • \u7c21\u55ae\uff0c\u5feb\u901f\uff0c\u9ad8\u901a\u91cf\u9069\u61c9\u6027 \u2022\u6e96\u78ba\uff1a\u53ef\u91cd\u8907\u7684\u7d50\u679c \u2022\u65b9\u4fbf\uff1a\u7121\u9700\u7279\u6b8a\u8655\u7406\u6216\u8655\u7406 \u2022\u9ad8\u9748\u654f\u5ea6\uff1a\u6bd4\u8272 \uff08\u5448\u8272\u5206\u6790\uff09 \u548c\u87a2\u5149\u683c\u5f0f \u2022\u591a\u529f\u80fd\uff1a\u8207\u5ee3\u6cdb\u7684\u6a23\u54c1\u985e\u578b\u76f8\u5bb9\uff0c\u5982\u7c98\u9644\u548c\u61f8\u6d6e\u7d30\u80de\uff0c\u7d44\u7e54\uff0c\u7d30\u80de\u57f9\u990a\u4e0a\u6e05\u6db2\uff0c\u98df\u7269\uff0c\u8840\u6e05\uff0c\u8840\u6f3f\uff0c\u5c3f\u6db2\u548c\u8a31\u591a\u5176\u4ed6\u751f\u7269\u6db2\u9ad4 \u2022\u975e\u5e38\u7a69\u5b9a\uff1a\u4fdd\u8cea\u671f\u9577<\/li>\n<\/ul>\n\n\n\n\n\n\n\n\n\n
    Cat # +Size<\/td>\nK716-100<\/td>\n<\/tr>\n
    Size<\/td>\n100 assays<\/td>\n<\/tr>\n
    Detection Method<\/td>\nAbsorbance at 450 nm<\/td>\n<\/tr>\n
    Species Reactivity<\/td>\nMammalian<\/td>\n<\/tr>\n
    Applications<\/td>\nN\/A<\/td>\n<\/tr>\n
    Features & Benefits<\/td>\n\u2022 Simple procedure; takes ~ less than 40 minutes \u2022 Fast and convenient<\/td>\n<\/tr>\n
    Kit Components<\/td>\n\u2022 Assay Buffer \u2022 Substrate \u2022 Developer \u2022 Enzyme Mix (200\u00b5l) \u2022 Cysteine \u2022 (NH\u2084)\u2082Fe(SO\u2084)\u2082 \u2022 Isocitrate Standard (10\u00b5mol)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    \u03b1–\u916e\u620a\u4e8c\u9178\u5448\u8272\/\u87a2\u5149\u6e2c\u5b9a\u8a66\u5291\u76d2–\u4e59\u919b\u9178\u5faa\u74b0\u5206\u6790\u5546\u54c1 | K677 -100 (ab8 […]<\/p>\n","protected":false},"author":3,"featured_media":14226,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[180],"tags":[3176],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14231"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14231"}],"version-history":[{"count":6,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14231\/revisions"}],"predecessor-version":[{"id":51189,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/14231\/revisions\/51189"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/14226"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14231"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14231"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14231"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}