{"id":13804,"date":"2017-09-08T14:37:20","date_gmt":"2017-09-08T06:37:20","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13804"},"modified":"2025-03-21T14:50:36","modified_gmt":"2025-03-21T06:50:36","slug":"%e6%b0%b8%e7%94%9f%e5%8c%96%e7%89%9b%e8%83%8e%e5%85%92%e5%8e%9f%e7%99%bc%e6%80%a7%e8%82%ba%e7%b4%b0%e8%83%9e-sv40-%e8%b2%a8%e8%99%9ft0091","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=13804","title":{"rendered":"\u6c38\u751f\u5316\u725b\u80ce\u5152\u539f\u767c\u6027\u80ba\u7d30\u80de-SV40 | \u547c\u5438\u9053\u75c5\u6bd2\u4e4b\u7d30\u80de\u6a21\u5f0f (\u8ca8\u865fT0091)"},"content":{"rendered":"

\u7d30\u80de\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)\u00a0<\/strong>\u6c38\u751f\u5316\u725b\u80ce\u5152\u539f\u767c\u6027\u80ba\u7d30\u80de-SV40 <\/strong>(<\/strong>\u8ca8\u865f<\/strong>T0091<\/strong>)<\/strong><\/p>\n

Immortalized Fetal Bovine Lung Cells – SV40<\/a><\/span><\/p>\n

\u6c38\u751f\u5316\u725b\u80ce\u5152\u539f\u767c\u6027\u80ba\u7d30\u80de-SV40\u662f\u7814\u7a76\u5404\u7a2e\u725b\u547c\u5438\u9053\u75c5\u6bd2\u7684\u6709\u7528\u7d30\u80de\u6a21\u5f0f\uff0c\u5305\u62ec\u725b\u526f\u6d41\u611f\u75c5\u6bd2\uff0c\u725b\u75c5\u6bd2\u6027\u8179\u7009\u75c5\u6bd2\u548c\u725b\u76b0\u75b9\u75c5\u6bd21\uff0c\u5c0d\u725b\u5065\u5eb7\u548c\u76f8\u95dc\u884c\u696d\u7522\u751f\u6975\u5927\u7684\u5f71\u97ff\u3002<\/p>\n

\"immortalized<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Organism<\/strong><\/td>\nBovine<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nLung<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Recommended Seeding Density<\/strong><\/td>\nThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
Immortalization Method<\/strong><\/td>\nSerial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6c38\u751f\u5316\u725b\u80ce\u5152\u539f\u767c\u6027\u80ba\u7d30\u80de-SV40 <\/strong>\u7d30\u80de\u7279\u6027: Adherent \/ Cobblestone-like | \u4f86\u6e90lung\u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>) \u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. Complete growth medium\u57f9\u990a\u6db2PriGrowIII(\u8ca8\u865fTM003<\/a>)\uff0c\u80ce\u725b\u8840\u6e05(Cat. No. TM999<\/a>)\u6700\u7d42\u6fc3\u5ea6\u70ba10%\u4e26\u586b\u52a01% Penicillin\/Streptomycin \u30021%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C\u3002<\/p>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/strong> <\/u><\/span>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u7d30\u80de\u7cfb\u5217\u5546\u54c1 (immortalized cell)\u00a0\u6c38\u751f\u5316\u725b\u80ce\u5152\u539f\u767c\u6027\u80ba\u7d30\u80de-SV40 (\u8ca8\u865fT0091) […]<\/p>\n","protected":false},"author":3,"featured_media":11653,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[453],"tags":[3215],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13804"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13804"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13804\/revisions"}],"predecessor-version":[{"id":50838,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13804\/revisions\/50838"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/11653"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13804"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13804"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13804"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}