{"id":13284,"date":"2017-08-27T20:03:47","date_gmt":"2017-08-27T12:03:47","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13284"},"modified":"2022-07-21T11:12:59","modified_gmt":"2022-07-21T03:12:59","slug":"%e4%ba%ba%e9%a1%9e%e5%ae%ae%e9%a0%b8%e8%a7%92%e8%b3%aa%e7%b4%b0%e8%83%9e%ef%bc%8816e6-e7-hck%ef%bc%89%ef%bd%9cimmortalized-human-cervical-keratinocytes-16e6e7-hck%e8%b2%a8%e8%99%9ft0659","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=13284","title":{"rendered":"\u4eba\u985e\u5bae\u9838\u89d2\u8cea\u7d30\u80de\uff0816E6 \/ E7 HCK\uff09\uff5cImmortalized Human Cervical Keratinocytes (16E6\/E7 HCK)\u8ca8\u865fT0659"},"content":{"rendered":"

HOT!!<\/b><\/span>\u00a0\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

\u6c38\u751f\u5316\u4eba\u985e\u5bae\u9838\u89d2\u8cea\u7d30\u80de\uff0816E6 \/ E7 HCK\uff09\uff5c\u8ca8\u865fT0659<\/a><\/span><\/p>\n

Immortalized Human Cervical Keratinocytes (16E6\/E7 HCK)<\/strong>\u6c38\u751f\u5316\u4eba\u985e\u5bae\u9838\u89d2\u5316\u7d30\u80de\uff0816E6 \/ E7 HCK\uff09\u4f86\u81ea\u5916\u79d1\u6a19\u672c\u7684\u6b63\u5e38\u4eba\u5916\u5bae\u9838\u7d30\u80de\uff0c\u4e26\u901a\u904e\u7528\u8868\u9054HPV-16 E7\u548cE6\u7684\u9006\u8f49\u9304\u75c5\u6bd2\u8f49\u67d3\u800c\u6c38\u751f\u5316\u3002 \u9019\u4e9b\u6c38\u751f\u5316\u7d30\u80de\u9069\u5408\u4f5c\u70baHPV-E6\u548cE7\u86cb\u767d\u7684\u8868\u9054\u4ee5\u53cap53\u548cRb\u5169\u8005\u7684\u81f4\u764c\u4f5c\u7528\u548c\u7d30\u80de\u8870\u8001\u7684\u6a21\u578b\uff0c\u5ef6\u9577\u4e86\u7d30\u80de\u5728\u6b63\u5e38\u7d30\u80de\u8870\u8001\u7684\u7b2c\u4e00\u500b\u95dc\u9375\u6642\u671f\u7684\u58fd\u547d\u3002 \u5177\u6709\u7aef\u7c92\u6062\u5fa9\u80fd\u529b\uff0c\u6c38\u751f\u5316\u4eba\u985e\u5bae\u9838\u89d2\u8cea\u5f62\u6210\u7d30\u80de\uff0816E6 \/ E7 HCK\uff09\u4e5f\u53ef\u7528\u65bc\u7814\u7a76\u5074\u91cd\u65bc\u4e86\u89e3\u7aef\u7c92\u7f3a\u5931\u548c\u6062\u5fa9\u5728HPV\u76f8\u95dc\u4eba\u985e\u764c\u75c7\u767c\u5c55\u4e2d\u7684\u4f5c\u7528\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Organism<\/strong><\/td>\nHomo sapiens<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nCervix<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nPolygonal<\/td>\n<\/tr>\n
Recommended Seeding Density<\/strong><\/td>\nThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
Immortalization Method<\/strong><\/td>\nTransfection with retrovirus expressing HPV-16 E7 and HPV-16 E6<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

HOT!!\u00a0\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c\u00a0|\u00a0DAPI\u67d3\u6838\u00a0|\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291\u00a0|\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291\u00a0|\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49 […]<\/p>\n","protected":false},"author":3,"featured_media":8019,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[455],"tags":[3288],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13284"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13284"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13284\/revisions"}],"predecessor-version":[{"id":13286,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13284\/revisions\/13286"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8019"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13284"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13284"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13284"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}