{"id":1324,"date":"2014-03-30T22:22:10","date_gmt":"2014-03-30T14:22:10","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=1324"},"modified":"2022-07-28T14:00:38","modified_gmt":"2022-07-28T06:00:38","slug":"abm_icumate_lentiviral","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=1324","title":{"rendered":"\u3010\u8a98\u5c0e\u8f09\u9ad4\u3011iCumate Lentiviral \u8868\u73fe\u7cfb\u7d71\u2014ABMgood"},"content":{"rendered":"<p><span style=\"font-size: medium; color: #008000;\"><b>\u6162\u75c5\u6bd2\u7c21\u4ecb<\/b><\/span><\/p>\n<p style=\"text-align: justify;\">\u3000\u3000\u6162\u75c5\u6bd2\uff08Lentivirus\uff09\u7684\u7d50\u69cb\u53ef\u5206\u6210\u5957\u819c\uff08envelop\uff09\u3001\u86cb\u767d\u8cea\u819c\uff08capsid\uff09\u3001\u4ee5\u53ca\u907a\u50b3\u7269\u8cea\u3002Lentivirus\u611f\u67d3\u5bbf\u4e3b\u5f8c\uff0c\u6703\u5c07\u5176\u57fa\u56e0\u5d4c\u5165\u5bbf\u4e3b\u7d30\u80de\u4f7f\u5176\u8868\u9054\u3002\u73fe\u70ba\u5be6\u9a57\u5ba4\u4e2d\u5e38\u898b\u7684\u57fa\u56e0\u91cd\u7d44\u5de5\u5177\u3002<\/p>\n<p style=\"text-align: justify;\">\u3000\u3000\u76ee\u524dlentivirus\u5df2\u53ef\u85c9\u7531\u3000<span style=\"color: #0000ff;\"><strong>1. \u5e36\u6709target gene\u7684lentivector<\/strong><\/span>\uff0c\u52a0\u4e0a\u3000<span style=\"color: #0000ff;\"><strong>2. packaging system<\/strong><\/span>\uff0c\u69cb\u6210\u7121\u81f4\u75c5\u6027\u7684psuedovirus\u3002<\/p>\n<p><span style=\"color: #008000; font-size: medium;\"><strong>\u00a0ABM iCumate Lentiviral \u6162\u75c5\u6bd2\u8a98\u5c0e\u8868\u73fe\u7cfb\u7d71<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\">\u3000\u3000\u91cd\u91d1\u5c6c\u3001steroid hormones\u3001\u6eab\u5ea6\u3001\u4ee5\u53ca\u6297\u751f\u7d20\uff08\u5982tetracycline\uff09\u5e38\u88ab\u5229\u7528\u4f5c\u70ba\u57fa\u56e0\u8868\u9054\u4e4b\u8a98\u5c0e\u7269\u3002ABM iCumate\u2122 Inducible Lentiviral Expression System\uff0c\u4f7f\u7528CuO \u4f5c\u70baoperator\uff08\u70baPseudomonas putida\u4e4bp-cym operon\uff09\uff0c\u8abf\u7bc0CMV promoter\u7684\u57fa\u56e0\u8868\u9054\u3002<\/p>\n<ul>\n<li>\u9ad8\u6548\u7387\u9032\u884ctransduction\uff1a\u4ee5<strong>\u6162\u75c5\u6bd2\u8f09<\/strong>\u9ad4\u4f5c\u70ba\u9aa8\u67b6\u3002<\/li>\n<li>\u9ad8\u5ea6\u57fa\u56e0\u8868\u9054\uff1a\u4f7f\u7528\u5f37\u529b\u4e4b<strong>CMV promoter<\/strong>\u3002<\/li>\n<li>\u4f7f\u7528<strong>Cumate<\/strong>\uff0c\u7c21\u55ae\u8abf\u63a7\u8a98\u5c0e\u7cfb\u7d71\u7684\u300e\u958b\u300f\u8207\u300e\u95dc\u300f\u3002<\/li>\n<li>No basal\/leaky gene expression<\/li>\n<li>\u53ef\u7528\u65bc\u4efb\u4f55\u4eba\u985e\u3001\u5c0f\u9f20\u3001\u5927\u9f20\u7684ORF\u3001siRNA\u3001\u53camiRNA lentiviral vector\u3002<\/li>\n<li>\u61c9\u7528\u5f48\u6027\u9ad8\uff1a\u5177<strong>All-in-One<\/strong>\u548c<strong>\u96d9\u8f09\u9ad4<\/strong>\u5169\u7a2e\u578b\u5f0f\u3002<\/li>\n<\/ul>\n<p><span style=\"color: #008000;\"><b>ABM iCumate\u2122 Inducible Lentiviral Expression System<\/b><b>\u5982\u4f55\u9032\u884c\u8abf\u7bc0\u57fa\u56e0\u8868\u9054\uff1a<\/b><\/span><b><\/b><\/p>\n<ul>\n<li>\u7576repressor\uff08CymR\uff09\u548c\u8a98\u5c0e\u7269Cumate\u7d50\u5408\uff0c\u5247CuO\u5448\u73fe<strong>\u300e\u958b\u300f<\/strong>\uff0c\u4f7fCMV\u5f97\u4ee5\u9032\u884c\u57fa\u56e0\u8868\u9054\u3002<\/li>\n<\/ul>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-full wp-image-1326\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/03\/iCumate_on.png\" alt=\"iCumate_on\" width=\"376\" height=\"180\" srcset=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/03\/iCumate_on.png 376w, https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/03\/iCumate_on-300x143.png 300w\" sizes=\"(max-width: 376px) 100vw, 376px\" \/><\/p>\n<ul>\n<li>\u7576\u74b0\u5883\u7f3a\u4e4f\u8a98\u5c0e\u7269Cumate\uff0c\u5247repressor\uff08CymR\uff09\u6703\u548coperator\uff08CuO\uff09\u9032\u884c\u7d50\u5408\uff0c\u9020\u6210iCumate\u8a98\u5c0e\u7cfb\u7d71<strong>\u300e\u95dc\u300f<\/strong>\uff0c\u505c\u6b62\u57fa\u56e0\u8868\u9054\u7684\u9032\u884c\u3002<\/li>\n<\/ul>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-full wp-image-1327\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/03\/iCumate_off.png\" alt=\"iCumate_off\" width=\"376\" height=\"118\" srcset=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/03\/iCumate_off.png 376w, https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/03\/iCumate_off-300x94.png 300w\" sizes=\"(max-width: 376px) 100vw, 376px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6162\u75c5\u6bd2\u7c21\u4ecb \u3000\u3000\u6162\u75c5\u6bd2\uff08Lentivirus\uff09\u7684\u7d50\u69cb\u53ef\u5206\u6210\u5957\u819c\uff08envelop\uff09\u3001\u86cb\u767d\u8cea\u819c\uff08capsid\uff09\u3001\u4ee5 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":1336,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[453,454,57,116,456,3],"tags":[4835],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/1324"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1324"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/1324\/revisions"}],"predecessor-version":[{"id":8550,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/1324\/revisions\/8550"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/1336"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1324"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1324"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1324"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}