{"id":13035,"date":"2017-08-21T17:41:54","date_gmt":"2017-08-21T09:41:54","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13035"},"modified":"2025-03-21T14:56:20","modified_gmt":"2025-03-21T06:56:20","slug":"cyp2c29%e7%a9%a9%e5%ae%9a%e5%b0%8f%e9%bc%a0%e8%82%9d%e7%b4%b0%e8%83%9e-cyp2c29-stable-mouse-hepatocytes-cell-line%e8%b2%a8%e8%99%9ft3010","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=13035","title":{"rendered":"Cyp2C29\u7a69\u5b9a\u5c0f\u9f20\u809d\u7d30\u80de | Cyp2C29 Stable Mouse Hepatocytes Cell Line\u8ca8\u865fT3010"},"content":{"rendered":"

A<\/strong>bmgood<\/strong>\u85e5\u7269\u7be9\u9078\u5e73\u53f0<\/strong>\u00a0|\u00a0<\/strong>\u7d30\u80de\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0(immortalized cell)<\/strong><\/span><\/p>\n

\"\"<\/p>\n

Cyp2C29<\/strong>\u7a69\u5b9a\u5c0f\u9f20\u809d\u7d30\u80de <\/strong>(<\/strong>\u8ca8\u865f<\/strong>T3010)\u00a0<\/strong>Cyp2C29 Stable Mouse Hepatocytes Cell Line<\/a><\/span><\/p>\n

\u7d30\u80de\u8272\u7d20P450\u86cb\u767d\uff08cytochrome P450 proteins , Cyps\uff09\u662f\u55ae\u52a0\u6c27\u9176\uff0c\u5176\u50ac\u5316\u6d89\u53ca\u85e5\u7269\u4ee3\u8b1d\u548c\u8102\u8cea\u5408\u6210\u7684\u53cd\u61c9\u3002 Cyp2c29\u662f\u4e00\u7a2e\u5728\u809d\u81df\u4e2d\u5c07\u82b1\u751f\u56db\u70ef\u9178\u50ac\u5316\u6210\u9806\u5f0f – \u74b0\u6c27\u4e8c\u5341\u78b3\u4e09\u70ef\u9178\uff08EET\uff09\u7684\u9f20\u985e\u86cb\u767d\u8cea\uff0c\u56e0\u5176\u5728\u4fe1\u865f\u50b3\u5c0e\u548c\u6297\u708e\u4e8b\u4ef6\u4e2d\u7684\u91cd\u8981\u6027\u800c\u805e\u540d\u3002 Cyp2c29\u7a69\u5b9a\u5c0f\u9f20\u809d\u7d30\u80de\u662f\u6e90\u81ea\u5c0f\u9f20\u809d\u7d30\u80de\u7684Cyp2c29\u8868\u9054\u7d30\u80de\u7cfb\u3002 qPCR\u8868\u9054\u6e2c\u5b9a\u8b49\u5be6\u4e86Cyp2c29\u7684\u8868\u9054\u3002 Cyp2c29\u7a69\u5b9a\u7684\u5c0f\u9f20\u809d\u7d30\u80de\u53ef\u4ee5\u662f\u85e5\u7269\u4ee3\u8b1d\u4e2d\u975e\u5e38\u6709\u7528\u7684\u7d30\u80de\u6a21\u578b\u3002<\/p>\n

Cyp2C29<\/strong>\u7a69\u5b9a\u5c0f\u9f20\u809d\u7d30\u80de <\/strong>\u7d30\u80de\u7279\u6027: Adherent \/ Polygonal | \u4f86\u6e90Liver\u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>)\u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2PriGrowII(\u8ca8\u865f\u00a0TM002)\uff0c\u00a02 mM L-glutamine (G275<\/a>)\uff0c\u80ce\u725b\u8840\u6e05(Cat. No. TM999<\/a>)\u6700\u7d42\u6fc3\u5ea6\u70ba10%\u4e26\u586b\u52a01% Penicillin\/Streptomycin \u30021%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C<\/p>\n\n\n\n\n\n\n\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Organism<\/strong><\/td>\nMus musculus<\/td>\n<\/tr>\n
SourceOrgan<\/strong><\/td>\nLiver<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nPolygonal<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

HOT!!<\/strong><\/span>\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Abmgood\u85e5\u7269\u7be9\u9078\u5e73\u53f0\u00a0|\u00a0\u7d30\u80de\u7cfb\u5217\u5546\u54c1\u00a0(immortalized cell) Cyp2C29\u7a69\u5b9a\u5c0f\u9f20\u809d […]<\/p>\n","protected":false},"author":3,"featured_media":11653,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[457],"tags":[3324],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13035"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13035"}],"version-history":[{"count":5,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13035\/revisions"}],"predecessor-version":[{"id":50843,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13035\/revisions\/50843"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/11653"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13035"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13035"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13035"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}