{"id":13027,"date":"2017-08-21T17:15:22","date_gmt":"2017-08-21T09:15:22","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13027"},"modified":"2025-03-21T14:56:50","modified_gmt":"2025-03-21T06:56:50","slug":"%e6%b0%a7%e5%8c%96%e9%82%84%e5%8e%9f%e9%85%b6%e7%a9%a9%e5%ae%9a%e7%b4%b0%e8%83%9e-hssaovap-1-a7r5-stable-cell-line-%e8%b2%a8%e8%99%9ft3155","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=13027","title":{"rendered":"\u6c27\u5316\u9084\u539f\u9176\u7a69\u5b9a\u7d30\u80de |hSSAO\/VAP-1 A7r5 Stable Cell Line \u8ca8\u865fT3155"},"content":{"rendered":"

A<\/strong>bmgood<\/strong>\u85e5\u7269\u7be9\u9078\u5e73\u53f0<\/strong> | <\/strong>\u7d30\u80de\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)<\/strong><\/span><\/p>\n

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\u6c27\u5316\u9084\u539f\u9176\u7a69\u5b9a\u7d30\u80de <\/strong>(<\/strong>\u8ca8\u865f<\/strong>T3155<\/span>)<\/strong><\/a>\u00a0<\/span>hSSAO\/VAP-1 A7r5 Stable Cell Line<\/p>\n

SSAO\uff08\u6c28\u57fa\u8132\u654f\u611f\u6027\u80fa\u6c27\u5316\u9176\uff09\u8207\u7cd6\u5c3f\u75c5\uff0c\u963f\u723e\u8328\u6d77\u9ed8\u75c5\uff0c\u52d5\u8108\u7ca5\u6a23\u786c\u5316\u548c\u4e2d\u98a8\u6709\u95dc\u3002\u56e0\u6b64\uff0c\u6709\u5fc5\u8981\u5177\u6709\u8868\u9054\u6240\u9700\u86cb\u767d\u8cea\u7684\u7d30\u80de\u7684\u53ef\u9760\u4f86\u6e90\uff0c\u7528\u65bc\u7814\u7a76\u5176\u5728\u9014\u5f91\u4e2d\u7684\u4f5c\u7528\u548c\u6d3b\u6027\u61c9\u7528\u3002 hSSAO \/ VAP-1 A7r5\u7a69\u5b9a\u7d30\u80de\u7cfb\u5728\u8cea\u819c\u4e2d\u7a69\u5b9a\u8868\u9054SSAO \/ VAP-1\uff0c\u4e26\u5c07SSAO\u91cb\u653e\u5230\u57f9\u990a\u57fa\u4e2d\u3002 \u8a72\u7d30\u80de\u4fc2\u4e0d\u986f\u793aMAO-A\u548cMAO-B\u6d3b\u6027\u3002 \u9019\u662fSSAO \/ VAP-1\u819c\u7d50\u5408\u5f62\u5f0f\u9176\u4f5c\u70ba\u6cbb\u7642\u76ee\u6a19\u7684\u7814\u7a76\u7684\u6709\u50f9\u503c\u7684\u4f86\u6e90\u3002<\/p>\n

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\u6c27\u5316\u9084\u539f\u9176\u7a69\u5b9a\u7d30\u80de <\/strong>\u7d30\u80de\u7279\u6027: Adherent \/ Fibroblast | \u4f86\u6e90Aorta, Thoracic Smooth Muscle \u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>) \u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2PriGrowIII(\u8ca8\u865f\u00a0TM003)\uff0c\u00a02 mM L-glutamine (G275<\/a>)\uff0c\u80ce\u725b\u8840\u6e05(Cat. No. TM999<\/a>)\u6700\u7d42\u6fc3\u5ea6\u70ba10%\u4e26\u586b\u52a01% Penicillin\/Streptomycin \u30021%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C<\/p>\n

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1) Selection via antibiotic G418; 2) Western blot used to determine presence of SSAO; 3) Activity of SSAO, MAO-A, and MAO-B was detected by radiochemical methods<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Organism<\/strong><\/td>\nRattus norvegicus<\/td>\n<\/tr>\n
SourceOrgan<\/strong><\/td>\nAorta, Thoracic Smooth Muscle<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nFibroblast<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

HOT!!<\/strong><\/span>\u00a0<\/u><\/span>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Abmgood\u85e5\u7269\u7be9\u9078\u5e73\u53f0 | \u7d30\u80de\u7cfb\u5217\u5546\u54c1 (immortalized cell) \u6c27\u5316\u9084\u539f\u9176\u7a69\u5b9a\u7d30\u80de (\u8ca8 […]<\/p>\n","protected":false},"author":3,"featured_media":11653,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[457],"tags":[3325],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13027"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13027"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13027\/revisions"}],"predecessor-version":[{"id":50844,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13027\/revisions\/50844"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/11653"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13027"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13027"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13027"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}