{"id":13020,"date":"2017-08-21T16:41:41","date_gmt":"2017-08-21T08:41:41","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=13020"},"modified":"2025-03-07T11:54:20","modified_gmt":"2025-03-07T03:54:20","slug":"ngf%e6%b4%bb%e6%80%a7%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-active-nerve-growth-factor-%e8%b2%a8%e8%99%9fapa105hu01","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=13020","title":{"rendered":"NGF\u6d3b\u6027\u91cd\u7d44\u86cb\u767d | Active Nerve Growth Factor \u8ca8\u865fAPA105Hu01"},"content":{"rendered":"

Active Nerve Growth Factor (NGF)<\/strong>\u6d3b\u6027<\/strong>\u91cd\u7d44\u86cb\u767d <\/strong>|<\/strong>\u8ca8\u865f<\/strong>APA105Hu01<\/a><\/span><\/p>\n

\u795e\u7d93\u751f\u9577\u56e0\u5b50\uff08NGF\uff09\u662f\u795e\u7d93\u71df\u990a\u56e0\u5b50\uff0c\u795e\u7d93\u80bd\u4e3b\u8981\u53c3\u8207\u67d0\u4e9b\u76ee\u6a19\u795e\u7d93\u5143\u7684\u751f\u9577(growth)\uff0c\u7dad\u6301(maintenance)\uff0c\u589e\u6b96(proliferation)\u548c\u5b58\u6d3b\u7684\u8abf\u7bc0\u3002 \u64da\u5831\u5c0e\uff0c\u7576\u55dc\u927b\u7d30\u80de\u7624\u7d30\u80de\u7cfbPC12(pheochromocytoma cell line ) \u66b4\u9732\u65bc\u795e\u7d93\u751f\u9577\u56e0\u5b50\uff08NGF\uff09\u6642\uff0c\u7d30\u80de\u901a\u904e\u505c\u6b62\u7d30\u80de\u5206\u88c2\u548c\u5ef6\u9577\u795e\u7d93\u7a81\uff08Greene\u548cTischler\uff0c1976\uff09\u3002 \u5c07\u7d30\u80de\u5728\u542b\u67095\uff05\u80ce\u725b\u8840\u6e05\u548c10\uff05\u99ac\u8840\u6e05\u7684Ham’s F12K\u4e0a\u751f\u9577\u5728\u805a\u8cf4\u6c28\u9178\u6216\u81a0\u539f\u5305\u88ab\u7684\u5e73\u677f\u4e0a\u3002 \u7576\u7d30\u80de\u9054\u5230\u5c0d\u6578\u671f\u751f\u9577\u6642\uff0c\u5c07\u65b0\u9bae\u57f9\u990a\u57fa\u820710ng \/ mL NGF\u4e00\u8d77\u52a0\u5165\uff0c\u7136\u5f8c\u6bcf\u5929\u7528\u5012\u7f6e\u986f\u5fae\u93e1\u89c0\u5bdf\u7d30\u80de\u3002\u7528NGF\uff0810ng \/ mL\uff09\u57f9\u990a6\u5929\u5f8cPC12\u7d30\u80de\u7684\u7d30\u80de\u5206\u88c2\u505c\u6b62\u548c\u5206\u5316\u5982\u57161\u6240\u793a\u3002\u4e0d\u542bNGF\u7684\u5c0d\u7167\u7d44\u4e0d\u986f\u793a\u795e\u7d93\u7a81\u751f\u9577\uff0c\u7d30\u80de\u8fc5\u901f\u7e41\u6b96\u3002<\/p>\n

\n
<\/div>\n
\u795e\u7d93\u751f\u9577\u56e0\u5b50\uff08NGF\uff09\u662f\u4e00\u7a2e\u795e\u7d93\u71df\u990a\u56e0\u5b50\u548c\u795e\u7d93\u80dc\u80bd\uff0c\u4e3b\u8981\u53c3\u8207\u8abf\u7bc0\u67d0\u4e9b\u76ee\u6a19\u795e\u7d93\u5143\u7684\u751f\u9577\u3001\u7dad\u6301\u3001\u589e\u6b96\u548c\u5b58\u6d3b\u3002\u64da\u5831\u5c0e\uff0c\u7576\u55dc\u927b\u7d30\u80de\u7624\u7d30\u80de\u7cfb PC12 \u66b4\u9732\u65bc\u795e\u7d93\u751f\u9577\u56e0\u5b50 (NGF) \u6642\uff0c\u7d30\u80de\u6703\u5728\u4e00\u5468\u5167\u505a\u51fa\u53cd\u61c9\uff0c\u505c\u6b62\u7d30\u80de\u5206\u88c2\u4e26\u5ef6\u9577\u795e\u7d93\u7a81\uff08Greene \u548c Tischler\uff0c1976\uff09\u3002\u7d30\u80de\u5728\u542b\u6709 5% \u80ce\u725b\u8840\u6e05\u548c 10% \u99ac\u8840\u6e05\u7684 Ham’s F12K \u4e2d\u5728\u805a\u8cf4\u6c28\u9178\u6216\u81a0\u539f\u86cb\u767d\u5305\u88ab\u7684\u5e73\u677f\u4e0a\u751f\u9577\u3002\u7576\u7d30\u80de\u9054\u5230\u5c0d\u6578\u751f\u9577\u671f\u6642\uff0c\u52a0\u5165\u65b0\u9bae\u57f9\u990a\u57fa\u548c10ng\/mL\u7684NGF\uff0c\u7136\u5f8c\u6bcf\u5929\u7528\u5012\u7f6e\u986f\u5fae\u93e1\u89c0\u5bdf\u7d30\u80de\u3002PC12\u7d30\u80de\u8207NGF(10ng\/mL)\u5b75\u80b26\u5929\u5f8c\u7d30\u80de\u5206\u88c2\u505c\u6b62\u8207\u5206\u5316\u5982\u57161\u6240\u793a\u3002\u672a\u63a5\u53d7NGF\u7684\u5c0d\u7167\u7d44\u672a\u986f\u793a\u795e\u7d93\u7a81\u751f\u9577\uff0c\u7d30\u80de\u589e\u751f\u8fc5\u901f\u3002<\/div>\n
<\/div>\n
NGF \u4e5f\u53c3\u8207\u8abf\u7bc0\u795e\u7d93\u5143\u751f\u547d\u9031\u671f\u4ee5\u5916\u7684\u9014\u5f91\u3002\u6b64\u5916\uff0c\u03b1-2-\u5de8\u7403\u86cb\u767d\uff08a2M\uff09\u5df2\u88ab\u9451\u5b9a\u70baNGF\u7684\u76f8\u4e92\u4f5c\u7528\u7269\uff0c\u56e0\u6b64\u9032\u884c\u7d50\u5408ELISA\u6e2c\u5b9a\u4f86\u6aa2\u6e2c\u91cd\u7d44\u4eba\u985eNGF\u548c\u91cd\u7d44\u4ebaa2M\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u7c21\u800c\u8a00\u4e4b\uff0cNGF \u5728\u542b\u6709 0.01% BSA\uff08pH 7.4\uff09\u7684 PBS \u4e2d\u9023\u7e8c\u7a00\u91cb\u3002\u7136\u5f8c\u5c07 100\u03bcL \u7684\u91cd\u8907\u6a23\u672c\u8f49\u79fb\u81f3 a2M \u5305\u88ab\u7684\u5fae\u91cf\u6ef4\u5b9a\u5b54\u4e2d\uff0c\u4e26\u5728 37\u2103 \u4e0b\u5b75\u80b2 2 \u5c0f\u6642\u3002\u7528PBST\u6d17\u5b54\u4e26\u8207\u6297-NGF pAb\u4e00\u8d77\u5b75\u80b21\u5c0f\u6642\uff0c\u7136\u5f8c\u5438\u4e7e\u4e26\u6d173\u6b21\u3002\u8207 HRP \u6a19\u8a18\u7684\u4e8c\u6297\u9ad4\u5b75\u80b2\u5f8c\uff0c\u5438\u4e7e\u5b54\u4e26\u6d17 3 \u6b21\u3002\u52a0\u5165\u5e95\u7269\u6eb6\u6db2\u5f8c\uff0c\u5c07\u5b54\u572837\u2103\u4e0b\u5b75\u80b215-25\u5206\u9418\u3002\u6700\u5f8c\uff0c\u5728\u5b54\u4e2d\u52a0\u5165 50\u03bcL \u7d42\u6b62\u6db2\u4e26\u7acb\u5373\u5728 450nm \u8655\u8b80\u53d6\u3002NGF\u548ca2M\u7684\u7d50\u5408\u6d3b\u6027\u5982\u57162\u6240\u793a\uff0c\u4e14\u6b64\u4f5c\u7528\u5448\u73fe\u5291\u91cf\u4f9d\u8cf4\u6027\u3002
\n\u6578\u5b57\u3002NGF\u8207a2M\u7684\u7d50\u5408\u6d3b\u6027<\/div>\n<\/div>\n

\u7528\u6cd5<\/span><\/h3>\n
\n

\u5728 20mM Tris\u3001150mM NaCl (pH8.0) \u4e2d\u91cd\u65b0\u914d\u88fd\u81f3\u6fc3\u5ea6 0.1-1.0 mg\/mL\u3002\u4e0d\u8981\u6e26\u65cb\u3002<\/p>\n<\/div>\n

\u8caf\u5b58<\/span><\/h3>\n
\n

\u907f\u514d\u91cd\u8907\u51b7\u51cd\/\u89e3\u51cd\u5faa\u74b0\u30022-8\u2103\u4fdd\u5b581\u500b\u6708\u3002\u7b49\u5206\u4e26\u5728-80\u00b0C \u4e0b\u4fdd\u5b58 12 \u500b\u6708\u3002<\/p>\n<\/div>\n

\u7a69\u5b9a<\/span><\/h3>\n
\n

\u71b1\u7a69\u5b9a\u6027\u7528\u640d\u5931\u7387\u4f86\u63cf\u8ff0\u3002\u640d\u5931\u7387\u900f\u904e\u52a0\u901f\u71b1\u964d\u89e3\u8a66\u9a57\u6e2c\u5b9a\uff0c\u5373\u5c07\u86cb\u767d\u8cea\u572837\u2103\u5b75\u80b248h\uff0c\u6c92\u6709\u89c0\u5bdf\u5230\u660e\u986f\u7684\u964d\u89e3\u548c\u6c88\u6fb1\u3002\u5728\u9069\u7576\u7684\u5132\u5b58\u689d\u4ef6\u4e0b\uff0c\u4fdd\u5b58\u671f\u9650\u5167\u640d\u5931\u7387\u5c0f\u65bc5%\u3002<\/p>\n<\/div>\n

\n
\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"<\/p>\n

\u9664\u4e86\u8abf\u7bc0\u795e\u7d93\u5143(neurons)\u751f\u547d\u9031\u671f(life cycle )\u7684\u9014\u5f91\u5916\uff0cNGF \u9084\u53c3\u8207\u5176\u4e2d\u3002 \u6b64\u5916\uff0cAlpha-2-\u5de8\u7403\u86cb\u767d (Alpha-2-Macroglobulin, a2M) \u5df2\u88ab\u78ba\u5b9a\u70ba NGF \u7684\u76f8\u4e92\u4f5c\u7528\u7269\uff0c\u56e0\u6b64\u9032\u884c\u4e86\u7d50\u5408 ELISA \u6e2c\u5b9a\u4ee5\u6aa2\u6e2c\u91cd\u7d44\u4eba NGF \u548c\u91cd\u7d44\u4eba a2M \u7684\u76f8\u4e92\u4f5c\u7528\u3002 \u7c21\u800c\u8a00\u4e4b\uff0c\u5c07 NGF \u5728\u542b\u6709 0.01% BSA (pH 7.4) \u7684 PBS \u4e2d\u9023\u7e8c\u7a00\u91cb\u3002 \u7136\u5f8c\u5c07 100\u03bcL \u7684\u91cd\u8907\u6a23\u54c1\u8f49\u79fb\u5230 2M \u5305\u88ab\u7684\u5fae\u91cf\u6ef4\u5b9a\u5b54\u4e2d\uff0c\u4e26\u5728 37\u2103\u4e0b\u5b75\u80b2 2 \u5c0f\u6642\u3002 \u7528 PBST \u6d17\u6ecc\u5b54\u4e26\u7528\u6297 NGF pAb \u5b75\u80b2 1 \u5c0f\u6642\uff0c\u7136\u5f8c\u5438\u51fa\u4e26\u6d17\u6ecc 3 \u6b21\u3002 \u8207 HRP \u6a19\u8a18\u7684\u4e8c\u6297\u5b75\u80b2\u5f8c\uff0c\u5438\u51fa\u5b54\u4e26\u6d17\u6ecc 3 \u6b21\u3002 \u52a0\u5165\u5e95\u7269\u6eb6\u6db2\u5f8c\uff0c\u5c07\u5b54\u5728 37\u2103\u4e0b\u5b75\u80b2 15-25 \u5206\u9418\u3002 \u6700\u5f8c\uff0c\u5411\u5b54\u4e2d\u52a0\u5165 50\u00b5L \u7d42\u6b62\u6db2\uff0c\u4e26\u7acb\u5373\u5728 450nm \u8655\u8b80\u6578\u3002 NGF\u8207a2M\u7684\u7d50\u5408\u6d3b\u6027\u5982\u57162\u6240\u793a\uff0c\u9019\u7a2e\u4f5c\u7528\u5448\u5291\u91cf\u4f9d\u8cf4\u6027\u3002<\/p>\n<\/div>\n

\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Cloud clone\u63d0\u4f9b\u539f\u6838\u53ca\u771f\u6838\u751f\u7269\u8868\u73fe\u4e4b\u91cd\u7d44\u86cb\u767d\u7cfb\u5217\u5546\u54c1\uff1b\u5176\u4e2dActive Glial Cell Line Derived Neurotrophic Factor (GDNF)\u6d3b\u6027\u91cd\u7d44\u86cb\u767d\uff0c\u70ba\u6d3b\u6027\u86cb\u767d\u3002\u7d14\u5ea6> 97%\uff0c\u63d0\u4f9b\u51cd\u4e7e\u578b\u5f0f\u5305\u88dd\u3002\u53ef\u61c9\u7528\u65bcCell culture; Activity Assays.\u7b49\u4e0d\u540c\u7814\u7a76\u3002\u86cb\u767d\u8cea\u4fdd\u5b58\u578b\u5f0f<\/strong>: \u86cb\u767d\u8cea\u6eb6\u65bc20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300 \u70ba\u51b7\u51cd\u4e7e\u71e5\u5305\u88dd\u3002\u4f7f\u7528\u524d20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg\/mL \u907f\u514dvortex. \u77ed\u671f\u4fdd\u5b58Store at 2-8\u00b0C for one month.\u5206\u88dd-80\u00b0C\u9577\u671f12 \u500b\u6708\u3002\u8acb\u907f\u514d\u53cd\u8986\u51b7\u51cd\u89e3\u51cd\u3002<\/p>\n

\"APA105Hu01\"<\/p>\n<\/div>\n<\/div>\n

USAGE<\/h3>\n
\n

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg\/mL. Do not vortex.<\/p>\n<\/div>\n

STORAGE<\/h3>\n
\n

Avoid repeated freeze\/thaw cycles. Store at 2-8\u00b0C for one month. Aliquot and store at -80\u00b0C for 12 months.<\/p>\n<\/div>\n

STABILITY<\/span><\/h3>\n
\n

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37\u00b0C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.<\/p>\n<\/div>\n

(NGF)<\/strong>\u6d3b\u6027\u91cd\u7d44\u86cb\u767d\u76f8\u95dc\u4e4b\u6297\u9ad4<\/strong> | <\/strong>\u86cb\u767d\u8cea<\/strong> \u7522\u54c1<\/strong><\/span><\/p>\n

\n
\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Catalog No.<\/td>\nRelated products for research use of Homo sapiens (Human) Organism species<\/td>\nApplications (RESEARCH USE ONLY!)<\/td>\n<\/tr>\n
RPA105Hu02<\/td>\nRecombinant Nerve Growth Factor (NGF)<\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
APA105Hu61<\/td>\nActive Nerve Growth Factor (NGF)<\/a><\/td>\nCell\u00a0culture;\u00a0Activity\u00a0Assays.<\/td>\n<\/tr>\n
RPA105Hu01<\/td>\nRecombinant Nerve Growth Factor (NGF)<\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
APA105Hu01<\/td>\nActive Nerve Growth Factor (NGF)<\/a><\/td>\nCell\u00a0culture;\u00a0Activity\u00a0Assays.<\/td>\n<\/tr>\n
EPA105Hu61<\/td>\nEukaryotic Nerve Growth Factor (NGF)<\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
PAA105Hu02<\/td>\nPolyclonal Antibody to Nerve Growth Factor (NGF)<\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
PAA105Hu01<\/td>\nPolyclonal Antibody to Nerve Growth Factor (NGF)<\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
PAA105Hu06<\/td>\nPolyclonal Antibody to Nerve Growth Factor (NGF)<\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
LAA105Hu71<\/td>\nBiotin-Linked Polyclonal Antibody to Nerve Growth Factor (NGF)<\/a><\/td>\nWB; IHC; ICC.<\/td>\n<\/tr>\n
MAA105Hu25<\/td>\nMonoclonal Antibody to Nerve Growth Factor (NGF)<\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
MAA105Hu27<\/td>\nMonoclonal Antibody to Nerve Growth Factor (NGF)<\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
MAA105Hu29<\/td>\nMonoclonal Antibody to Nerve Growth Factor (NGF)<\/a><\/td>\nIHC<\/td>\n<\/tr>\n
MAA105Hu21<\/td>\nMonoclonal Antibody to Nerve Growth Factor (NGF)<\/a><\/td>\nWB<\/td>\n<\/tr>\n
MAA105Hu22<\/td>\nMonoclonal Antibody to Nerve Growth Factor (NGF)<\/a><\/td>\nWB,ICC\/IF<\/td>\n<\/tr>\n
MAA105Hu24<\/td>\nMonoclonal Antibody to Nerve Growth Factor (NGF)<\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
MAA105Hu23<\/td>\nMonoclonal Antibody to Nerve Growth Factor (NGF)<\/a><\/td>\nWB<\/td>\n<\/tr>\n
FAA105Hu02<\/td>\nAnti-Nerve Growth Factor (NGF) Monoclonal Antibody<\/a><\/td>\nFlow cytometry.<\/td>\n<\/tr>\n
SEA105Hu<\/td>\nELISA Kit for Nerve Growth Factor (NGF)<\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
HEA105Hu<\/td>\nHigh Sensitive ELISA Kit for Nerve Growth Factor (NGF)<\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
SCA105Hu<\/td>\nCLIA Kit for Nerve Growth Factor (NGF)<\/a><\/td>\nChemiluminescent immunoassay for Antigen Detection.<\/td>\n<\/tr>\n
LMA105Hu<\/td>\nMultiplex Assay Kit for Nerve Growth Factor (NGF) ,etc. by FLIA (Flow Luminescence Immunoassay)<\/a><\/td>\nFLIA Kit for Antigen Detection.<\/td>\n<\/tr>\n
KSA105Hu01<\/td>\nELISA Kit DIY Materials for Nerve Growth Factor (NGF)<\/a><\/td>\nMain materials for “Do It (ELISA Kit) Yourself”.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
<\/div>\n<\/div>\n

\"CUBEE\u684c\u4e0a\u578b\u5c0f\u578b\u96e2\u5fc3\u6a5f\"<\/a>\"VORTEX\"<\/a>\"BIODROP-\u5be6\u9a57\u8a2d\u50993\"\"ELISA<\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Active Nerve Growth Factor (NGF)\u6d3b\u6027\u91cd\u7d44\u86cb\u767d |\u8ca8\u865fAPA105Hu01 \u795e\u7d93 […]<\/p>\n","protected":false},"author":3,"featured_media":38092,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5377,461],"tags":[3326],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13020"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13020"}],"version-history":[{"count":10,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13020\/revisions"}],"predecessor-version":[{"id":50693,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/13020\/revisions\/50693"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/38092"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13020"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13020"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13020"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}