{"id":12814,"date":"2017-08-15T11:02:04","date_gmt":"2017-08-15T03:02:04","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=12814"},"modified":"2023-05-03T10:20:18","modified_gmt":"2023-05-03T02:20:18","slug":"%e7%9a%ae%e8%b3%aa%e9%86%87%ef%bc%88%e5%94%be%e6%b6%b2%ef%bc%89%e8%a9%a6%e5%8a%91%e7%9b%92-cortisol-saliva-human-elisa-kit%e8%b2%a8%e8%99%9fk7434","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=12814","title":{"rendered":"\u76ae\u8cea\u9187\uff08\u553e\u6db2\uff09\u8a66\u5291\u76d2 | Cortisol (Saliva) Human ELISA Kit\u8ca8\u865fK7434 (ab285353)"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=12814\" target=\"_blank\" rel=\"noopener\"><strong>\u76ae\u8cea\u9187\uff08\u553e\u6db2\uff09\u4eba\u9ad4ELISA\u8a66\u5291\u76d2 <\/strong>\u00a0(Saliva) | \u8ca8\u865fK7434<\/a>\u00a0(ab285353)<\/span><\/p>\n<p><strong><em>Sensitive, Colorimetric Assay<\/em><\/strong><\/p>\n<p>BioVision\u7684\u76ae\u8cea\u9187\uff08\u553e\u6db2\uff09\u9176\u806f\u514d\u75ab\u5438\u9644\u6e2c\u5b9a\uff08ELISA\uff09\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u9ad4\u5916\u6e2c\u5b9a\u553e\u6db2\u4e2d\u76ae\u8cea\u9187\u7684\u5b9a\u91cf\u6e2c\u5b9a\u3002\u76ae\u8cea\u9187\u662f\u4e00\u7a2e\u5f9e\u6fc0\u7d20ACTH\u5f15\u8d77\u7684\u5f9e\u814e\u4e0a\u817a\u76ae\u8cea\u91cb\u653e\u7684\u985e\u56fa\u9187\u6fc0\u7d20\u3002\u5b83\u53ef\u4ee5\u589e\u52a0\u8840\u58d3\u548c\u8840\u7cd6\u6c34\u5e73\uff0c\u4e26\u6291\u5236\u514d\u75ab\u7cfb\u7d71\u3002\u76ae\u8cea\u9187\u901a\u904e\u7279\u5b9a\u7684\u7d30\u80de\u5167\u53d7\u9ad4\u8d77\u4f5c\u7528\uff0c\u4e26\u4e14\u5c0d\u8a31\u591a\u751f\u7406\u7cfb\u7d71\u6709\u5f71\u97ff\uff0c\u5305\u62ec\u514d\u75ab\u7cfb\u7d71\uff0c\u8461\u8404\u7cd6\u5c0d\u6297\u8abf\u7bc0\uff0c\u8840\u7ba1\u7dca\u5f35\u5ea6\uff0c\u5e95\u7269\u5229\u7528\u548c\u9aa8\u4ee3\u8b1d\u3002\u5b83\u4f5c\u70ba\u6e38\u96e2\u5f62\u5f0f\u5b58\u5728\u65bc\u8840\u6db2\u4e2d\uff0c\u6216\u8207\u76ae\u8cea\u985e\u56fa\u9187\u7d50\u5408\u7403\u86cb\u767d\uff08CBG\uff09\u7d50\u5408\u3002\u8840\u6db2\u4e2d\u53ea\u67091-10\uff05\u7684\u76ae\u8cea\u9187\u4ee5\u672a\u7d50\u5408\/\u6e38\u96e2\u7684\u5f62\u5f0f\u5b58\u5728\uff0c\u9019\u4e00\u90e8\u5206\u4ee3\u8868\u4e86\u5f15\u8d77\u751f\u7269\u53cd\u61c9\u7684\u76ae\u8cea\u9187\u7684\u6d3b\u6027\u6c60\uff0c\u4e26\u4e14\u80fd\u5920\u64f4\u6563\u5230\u9776\u7d44\u7e54\u548c\u553e\u6db2\u4e2d\u3002\u7814\u7a76\u4e00\u81f4\u5831\u5c0e\u553e\u6db2\u548c\u8840\u6e05\u76ae\u8cea\u9187\u6c34\u5e73\u4e4b\u9593\u9ad8\u5ea6\u76f8\u95dc\uff0c\u8868\u660e\u553e\u6db2\u76ae\u8cea\u9187\u53ef\u4ee5\u53ef\u9760\u5730\u4f30\u8a08\u8840\u6e05\u76ae\u8cea\u9187\u6c34\u5e73\u3002 BioVision\u7684\u76ae\u8cea\u9187\uff08Saliva\uff09ELISA\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u7af6\u722d\u6027\u9176\u514d\u75ab\u6e2c\u5b9a\u8a66\u5291\u76d2\u3002\u8a72\u6e2c\u5b9a\u6cd5\u4f7f\u7528\u5857\u8986\u572896\u5b54\u677f\u4e0a\u7684\u76ae\u8cea\u9187\u7279\u7570\u6027\u6297\u9ad4\u3002\u6a23\u54c1\u4e2d\u7684\u76ae\u8cea\u9187\u548c\u6a19\u8a18\u70ba\u76ae\u8cea\u9187\u7684HRP\u7af6\u722d\u7d50\u5408\u56fa\u5b9a\u7684\u6297\u9ad4\u3002\u540c\u6642\u5b75\u80b2\u5f8c\uff0c\u5c07\u591a\u9918\u7684\u8a66\u5291\u6d17\u53bb\uff0c\u4e26\u5c07a B\u57fa\u8cea\u52a0\u5165\u5b54\u4e2d\u3002\u984f\u8272\u8207\u7d50\u5408\u7684\u76ae\u8cea\u9187HRP\u7db4\u5408\u7269\u7684\u91cf\u6210\u6bd4\u4f8b\u5730\u767c\u751f\uff0c\u4e26\u4e14\u5f37\u5ea6\u8207\u6a23\u54c1\u4e2d\u76ae\u8cea\u9187\u7684\u6fc3\u5ea6\u6210\u53cd\u6bd4\u3002 Stop Solution\u5c07\u984f\u8272\u5f9e\u85cd\u8272\u8b8a\u70ba\u9ec3\u8272\uff0c\u4e26\u4e14\u5728450nm\u8655\u6e2c\u91cf\u984f\u8272\u7684\u5f37\u5ea6\u3002\u8a66\u5291\u76d2\u7684\u9748\u654f\u5ea6\u70ba1 ng \/ ml\uff0c\u6aa2\u6e2c\u7bc4\u570d\u70ba1 ng \/ ml\u81f3100 ng \/ ml\u3002\u8a72ELISA\u6e2c\u5b9a\u7684\u4ea4\u53c9\u53cd\u61c9\u6027\u70ba\u00a0prednisolone\u70ba6.8\uff05\uff0cCortisone\u70ba4.22\uff05\uff0c\u5176\u4ed6\u6fc0\u7d20\u70ba0.1\uff05\uff0c\u5305\u62ec11-deoxycortisol\uff0c\u00a0estradiol\uff0c\u00a0testosterone\u548cprogesterone\u3002<\/p>\n<p><span style=\"color: #ff6600;\"><span style=\"color: #008080;\">\u5546\u54c1\u9700\u7528\u5100\u5668\u6e2c\u91cf<\/span>\u00a0\u00a0<a style=\"color: #ff6600;\" href=\"http:\/\/biopioneer.com.tw\/?p=25739\">\u9175\u7d20\u514d\u75ab\u5206\u6790\u5100\u8acb\u6309\u6b64\u00a0<\/a><\/span>(405\u3001450\u3001492\u3001630 nm)<\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/?p=25739\"><img decoding=\"async\" loading=\"lazy\" class=\"wp-image-25744 alignleft\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2020\/08\/RT-6900.png\" alt=\"RT-6900\" width=\"223\" height=\"236\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/k\/7\/k7434.jpg\" alt=\"\" width=\"691\" height=\"343\" \/><\/p>\n<table width=\"838\">\n<tbody>\n<tr>\n<td><strong>Cat # +Size<\/strong><\/td>\n<td>K7434-100<\/td>\n<\/tr>\n<tr>\n<td><strong>Size<\/strong><\/td>\n<td>100 assays<\/td>\n<\/tr>\n<tr>\n<td><strong>Detection Method<\/strong><\/td>\n<td>Absorbance (450 nm)<\/td>\n<\/tr>\n<tr>\n<td><strong>Species Reactivity<\/strong><\/td>\n<td>human<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>Quantitative detection of Cortisol<\/td>\n<\/tr>\n<tr>\n<td><strong>Features &amp; Benefits<\/strong><\/td>\n<td>\u2022 Easy, convenient and time-saving method to measure cortisol<br \/>\n\u2022 Sensitivity: 1 ng\/ml and detection range: 1 ng\/ml to 100 ng\/ml<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Components<\/strong><\/td>\n<td>\u2022 Plate Coated with Cortisol Ab<br \/>\n\u2022 Assay Diluent<br \/>\n\u2022 Wash Buffer (10x)<br \/>\n\u2022 Standard Diluent<br \/>\n\u2022 Cortisol Standard (4800 ng\/ml)<br \/>\n\u2022 Cortisol HRP Conjugate<br \/>\n\u2022 \u2122B Substrate<br \/>\n\u2022 Stop Solution<br \/>\n\u2022 Plate Sealer<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u00a0\u5176\u5b83\u4ee3\u8b1d\u7522\u54c1\u5206\u6790<\/strong><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/biovision%E5%95%86%E5%93%81%E6%8F%92%E5%9C%96-300x207.jpg\" alt=\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\" width=\"243\" height=\"158\" \/><\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/?p=6035\"><strong>\u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790\u5957\u7d44<\/strong> <\/a><strong>| <\/strong><a href=\"http:\/\/biopioneer.com.tw\/?p=6031\"><strong>\u80c6\u56fa\u9187\u5206\u6790\u5957\u7d44<\/strong><\/a><strong> | <\/strong><a href=\"http:\/\/biopioneer.com.tw\/?p=6038\"><strong>\u8102\u8cea\u7d30\u80de\u67d3\u8272\u5291<\/strong><\/a><strong> | <\/strong><a href=\"http:\/\/biopioneer.com.tw\/?p=6040\"><strong>\u9577\u93c8\u6e38\u96e2\u8102\u80aa\u9178\u5206\u6790\u5957\u7d44<\/strong><\/a><strong> | <\/strong><a href=\"http:\/\/biopioneer.com.tw\/?p=5741\"><strong>TG ELISA<\/strong><strong>\u5957\u7d44<\/strong><\/a><\/p>\n<p><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=12814\" target=\"_blank\" rel=\"noopener\"><strong>\u76ae\u8cea\u9187\uff08\u553e\u6db2\uff09\u4eba\u9ad4ELISA\u8a66\u5291\u76d2\u00a0<\/strong><\/a>\u64cd\u4f5c\u65b9\u5f0f\uff1a<\/p>\n<p>1.<strong>\u6a23\u54c1\u88fd\u5099\uff1a<\/strong>\u6536\u96c6\u553e\u6db2\u6a23\u54c1\u4e26\u51b7\u51cd\/\u89e3\u51cd\u81f3\u5c11\u4e00\u6b21\u3002\u5728\u5ba4\u6eab\u4e0b3000xg\u96e2\u5fc35\u5206\u9418\u3002\u53bb\u9664\u6a23\u54c1\u4e2d\u7684Pellet\uff08\u7c98\u86cb\u767d\uff09\u3002\u6536\u96c6\u6e05\u6f88\u7684\u4e0a\u6e05\u6db2\u4ee5\u5099\u6e2c\u91cf\u3002<\/p>\n<p>2.<strong>\u8a66\u5291\u88fd\u5099:<\/strong> \u5c07\u6240\u6709\u7de9\u885d\u6db2\u548c\u6240\u9700\u6578\u91cf\u7684Coated strips\u81f3\u5ba4\u5167\u6eab\u5ea6\uff0818-25\u00b0C\uff09\u56de\u6eab\u3002\u5efa\u8b70\u6a19\u6e96\u7a00\u91cb\u6db2\u4e8c\u91cd\u8986\u3002<\/p>\n<p>3.<strong>\u6a19\u6e96\u54c1\u88fd\u5099:<\/strong> \u6e96\u5099\u4e00\u7cfb\u5217\u7a00\u91cb\u5291\uff0c\u7528\u65bc\u76ae\u8cea\u9187\u6a19\u6e96\u54c1\uff084800 ng \/ ml\uff09\u6a19\u6e96\u7a00\u91cb\u5291\uff0c\u5982\u57161\u6240\u793a\u3002\u5728\u8f49\u79fb\u4e4b\u524d\uff0c\u7686\u8f15\u8f15\u5730\u5fb9\u5e95\u6df7\u5408\u8a66\u7ba1\uff0c\u4ee5\u78ba\u4fdd\u6a19\u6e96\u6db2\u70ba\u6df7\u5408\u5747\u52fb\u72c0\u614b\u3002\u6a19\u6e96\u7a00\u91cb\u5291\u7528\u4f5c\u96f6\u6a19\u6e96\uff080ng \/ ml\uff09\u3002<\/p>\n<p>4. \u79fb\u53d620\u03bcl\u76ae\u8cea\u9187\u6a19\u6e96\u54c1\u6216\u6a23\u54c1\u5230\u5404Well\u4e2d\u3002<\/p>\n<p>5. \u88fd\u50991x\u76ae\u8cea\u9187HRP\u7d50\u5408\u7269\u6eb6\u6db2\uff081000x\uff09: \u4ee5Assay Diluent\u7a00\u91cb\u70ba1X\u3002\u76e1\u53ef\u80fd\u591a\u505a\u4e00\u4e9b\u3002\u52a0\u5165100\u03bcl1x\u76ae\u8cea\u9187HRP\u7db4\u5408\u7269\u53ca\u6eab\u548c\u5730Shaker\u3002\u7528Plate Sealer\u84cb\u4e0a\u5b54\u4e26\u6eab\u548c\u6416\u52d5\u5b75\u80b25\u5206\u9418\u3002\u5ba4\u6eab\u572837\u00b0C\u5b75\u80b21\u5c0f\u6642\u3002<\/p>\n<p>6.\u79fb\u9664\u6eb6\u6db2\u5f8c\u4ee5200\u03bcl1x\u6d17\u6ecc\u7de9\u885d\u6db2\u8f15\u8f15\u6416\u52d5\u6d17\u6ecc4\u6b21\uff08\u6bcf\u6b213-4\u5206\u9418\uff09\u3002<\/p>\n<p>7.\u52a0\u5165100\u03bclTMB\u5e95\u7269\/\u5b54\u4e26\u8f15\u8f15\u6416\u52d5\u3002\u6e2c\u91cf650nm\u8655\u7684\u5438\u5149\u5ea61-4\u5206\u9418\u3002\u5728\u5ba4\u6eab\u4e0b\u76e3\u6e2c\u85cd\u8272\u986f\u8272\uff0c\u5176\u5f37\u5ea6\u8207\u6a23\u54c1\u548c\u6a19\u6e96\u54c1\u4e2d\u76ae\u8cea\u9187\u7684\u6fc3\u5ea6\u6210\u53cd\u6bd4\u3002<\/p>\n<p><span style=\"color: #008000;\">\u6ce8\u610f\u4e8b\u9805: \u6dfb\u52a0TMB\u5e95\u7269\u5f8c\u7684\u5b75\u80b2\u6642\u9593\u53ef\u4ee5\u512a\u5316\uff0c\u4ee5\u907f\u514d\u984f\u8272\u904e\u5ea6\u767c\u5c55\u3002\u63a8\u85a6\u7684\u5438\u5149\u5ea60ng \/ ml\u76ae\u8cea\u9187\u6a19\u6e96\u54c1\u5728650nm\u8655\u70ba~0.5-0.8\u3002\u6700\u4f73\u5316\uff1a\u6e96\u5099\u4e00\u500b\u5e73\u884c\u5b54\u7528\u65bc\u80cc\u666f\u63a7\u5236\u4e26\u6dfb\u52a0TMB\u5e95\u7269\u3002<\/span><\/p>\n<p>8.\u5411\u6bcf\u500b\u5b54\u4e2d\u52a0\u5165100\u03bcl\u7d42\u6b62\u6db2\uff0c\u5305\u62ec\u80cc\u666f\u5c0d\u7167\uff0c\u8f15\u8f15\u6416\u52d5\u6df7\u5408\u3002\u82e5\u6709\u6c23\u6ce1, \u5247\u53bb\u9664\u6c23\u6ce1\u3002\u57285\u5206\u9418\u5167\u6e2c\u91cf450nm\u8655\u7684\u5438\u5149\u5ea6\u3002<\/p>\n<p>9.\u8a08\u7b97\uff1a\u8a08\u7b97\u6bcf\u7d44\u91cd\u8907\u6a19\u6e96\u54c1\u7684\u5e73\u5747\u5438\u5149\u5ea6\u3002\u7e6a\u88fd\u76ae\u8cea\u9187\u6a19\u6e96\u66f2\u7dda\u3002\u8a08\u7b97\u76ae\u8cea\u9187\u901a\u904e\u6a19\u6e96\u66f2\u7dda\u7684\u63d2\u503c\u4f86\u6fc3\u7e2e\u6a23\u54c1\u3002\u5982\u679c\u7a00\u91cb\u6a23\u54c1\uff0c\u5247\u5c07\u8a72\u503c\u4e58\u4ee5\u7a00\u91cb\u56e0\u5b50\u9032\u884c\u8a08\u7b97\u6a23\u54c1\u4e2d\u76ae\u8cea\u9187\u7684\u6fc3\u5ea6\u3002<\/p>\n<p>\u6ce8\u610f\uff1a\u6bcf\u6b21\u8b80\u6578\u7684\u80cc\u666f\u6263\u9664\u5c0d\u65bc\u8a08\u7b97\u6a23\u54c1\u76ae\u8cea\u9187\u6fc3\u5ea6\u662f\u53ef\u9078\u7684\uff0c\u4e26\u4e14\u4e0d\u6703\u6539\u8b8a\u6700\u7d42\u7d50\u679c\u3002<\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2022\/10\/\u76ae\u8cea\u9187\uff08\u553e\u6db2\uff09\u8a66\u5291\u76d2-Cortisol-Saliva-Human-ELISA-Kit\u8ca8\u865fK7434-ab285353.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E6%93%8D%E4%BD%9C%E6%89%8B%E5%86%8A2-300x95.jpg\" alt=\"\u64cd\u4f5c\u624b\u518a2\" width=\"140\" height=\"43\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u76ae\u8cea\u9187\uff08\u553e\u6db2\uff09\u4eba\u9ad4ELISA\u8a66\u5291\u76d2 \u00a0(Saliva) | \u8ca8\u865fK7434\u00a0(ab285353) Sensit [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[493],"tags":[3356],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12814"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=12814"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12814\/revisions"}],"predecessor-version":[{"id":42004,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12814\/revisions\/42004"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=12814"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=12814"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=12814"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}