{"id":12638,"date":"2017-08-10T17:24:25","date_gmt":"2017-08-10T09:24:25","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=12638"},"modified":"2025-03-21T14:57:16","modified_gmt":"2025-03-21T06:57:16","slug":"nf-kb%e8%9e%a2%e5%85%89%e7%b4%a0%e9%85%b6%e7%a9%a9%e5%ae%9araw264-7%e7%b4%b0%e8%83%9e%e6%a0%aa-nf-kb-luciferase-stable-raw264-7-cell-line-%e8%b2%a8%e8%99%9ft3015","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=12638","title":{"rendered":"NF-kB\u87a2\u5149\u7d20\u9176\u7a69\u5b9aRAW264.7\u7d30\u80de\u682a |NF-kB Luciferase Stable RAW264.7 Cell Line \u8ca8\u865fT3015"},"content":{"rendered":"

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)<\/strong><\/p>\n

NF-kB<\/strong>\u87a2\u5149\u7d20\u9176\u7a69\u5b9aRAW264.7\u7d30\u80de<\/strong>\u682a<\/strong> (<\/strong>\u8ca8\u865f<\/strong>T3015)\u00a0<\/strong>NF-kB Luciferase Stable RAW264.7 Cell Line<\/a><\/span><\/p>\n

\u87a2\u5149\u7d20\u9176\u901a\u5e38\u7528\u4f5c\u5831\u544a\u57fa\u56e0\uff0c\u4ee5\u8a55\u4f30\u611f\u8208\u8da3\u7684Promoter\u555f\u52d5\u5b50\u4e0b\u4e4b\u87a2\u5149\u7d20\u9176\u57fa\u56e0transcriptional activity\u8f49\u9304\u6d3b\u6027\u3002 NF-kB\u87a2\u5149\u7d20\u9176\u7a69\u5b9a\u8868\u73fe\u4e4b\u7684RAW264.7\u7d30\u80de\u682a\uff0c\u51773kB-Luc SV40\u5831\u5c0e\u57fa\u56e0\u7a69\u5b9a\u8f49\u67d3\uff0c\u8a72\u5831\u544a\u57fa\u56e0\u5305\u542b\u4f86\u81ea\u87a2\u5149\u7d20\u9176\u7de8\u78bc\u5340\u4e0a\u6e38\u5e72\u64fe\u7d20\u57fa\u56e0\u76843\u500bNF-kB\u4f4d\u9ede\u3002 \u8a72\u7d30\u80de\u4fc2\u53ef\u7528\u65bc\u55ae\u6838\u7d30\u80de – \u5de8\u566c\u7d30\u80de\u6a21\u578b\u4e2dNF-kB\u6d3b\u5316\u4e4b\u7814\u7a76\u3002<\/p>\n

\"\"<\/p>\n

NF-kB<\/strong>\u87a2\u5149\u7d20\u9176\u7a69\u5b9aRAW264.7\u7d30\u80de<\/strong>\u682a\u4f86\u6e90<\/strong>Ascites \u751f\u9577\u578b\u614bAdherent | \u4e0d\u6747\u65b9\u6cd5: hTERT | \u4f86\u6e90Mus musculus Ascites\u3002\u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>) \u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2PriGrowIII(\u8ca8\u865f\u00a0TM003)\uff0c\u80ce\u725b\u8840\u6e05(Cat. No. TM999<\/a>)\u6700\u7d42\u6fc3\u5ea6\u70ba10%\u4e26\u586b\u52a01% Penicillin\/Streptomycin\u3001 L-Glutamine to 2mM\u3002100ug\/ml G418\u3002Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Organism<\/strong><\/td>\nMus musculus<\/td>\n<\/tr>\n
SourceOrgan<\/strong><\/td>\nAscites<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nMonocyte\/macrophage<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

HOT!!<\/strong><\/span>\u00a0<\/u><\/span>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Abmgood\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1 (immortalized cell) NF-kB\u87a2\u5149\u7d20\u9176\u7a69\u5b9aRAW264.7\u7d30 […]<\/p>\n","protected":false},"author":3,"featured_media":11653,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[453],"tags":[3381],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12638"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=12638"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12638\/revisions"}],"predecessor-version":[{"id":50845,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12638\/revisions\/50845"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/11653"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=12638"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=12638"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=12638"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}