{"id":12465,"date":"2017-08-04T12:49:48","date_gmt":"2017-08-04T04:49:48","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=12465"},"modified":"2022-07-21T11:30:29","modified_gmt":"2022-07-21T03:30:29","slug":"biotin-hpdp-n-6-%ef%bc%88%e7%94%9f%e7%89%a9%e7%b4%a0%e9%85%b0%e8%83%ba%ef%bc%89%e5%b7%b1%e5%9f%ba-3-%ef%bc%882-%e5%90%a1%e5%95%b6%e5%9f%ba%e4%ba%8c%e7%a1%ab%e4%bb%a3%ef%bc%89-%e4%b8%99","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=12465","title":{"rendered":"Biotin-HPDP | N- [6-\uff08\u751f\u7269\u7d20\u9170\u80fa\uff09\u5df1\u57fa]-3′ – \uff082′-\u5421\u5576\u57fa\u4e8c\u786b\u4ee3\uff09 – \u4e19\u9170\u80fa \u8ca8\u865f2329"},"content":{"rendered":"

Crosslinking<\/strong>\u8a66\u5291\u7cfb\u5217\u5546\u54c1<\/strong><\/p>\n

Biotin-HPDP \u00a0N- [6-\uff08\u751f\u7269\u7d20\u9170\u80fa\uff09\u5df1\u57fa]-3′ – \uff082′-\u5421\u5576\u57fa\u4e8c\u786b\u4ee3\uff09 – \u4e19\u9170\u80fa | <\/strong>\u8ca8\u865f<\/strong>2329<\/a><\/span><\/p>\n

\"\"<\/p>\n

A sulfhydryl reactive biotinylation reagentt<\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> HPDP\u542b\u6709\u5c0d\u5df0\u57fa\uff08-SH\uff09\u5982\u534a\u80f1\u6c28\u9178\uff08C\uff09\u5074\u93c8\u5177\u6709\u53cd\u61c9\u6027\u7684\u4e8c\u786b\u4ee3\u5421\u5576\u57fa\uff0c\u4ee5\u5f62\u6210\u7a69\u5b9a\u7684\u4e8c\u786b\u9375\uff0c\u542b\u6709\u9577\u5ea6\u70ba29.2 A\u030a\u7684spacer arm\u9593\u9694\u81c2\uff0c\u9632\u6b62\u7a7a\u9593\u4f4d\u963b\u5c0d\u751f\u7269\u7d20\u6a19\u8a18\u3002Spacersy\u65bc \u4e8c\u786b\u9375\u53ef\u88ab\u9084\u539f\u5291\u5207\u5272\uff0c\u5982DTT\uff0cTCEP\u7b49\u9084\u539f\u5291\u3002ASB-14\u5fc5\u9808\u6eb6\u89e3\u5728\u6709\u6a5f\u6eb6\u5291\u5982DMF\u6216DMSO\u4e2d\uff0c\u7136\u5f8c\u7a00\u91cb\u6c34\u6eb6\u6db2\u7de9\u885d\u6db2\u3002\u9069\u7528\u65bc\u751f\u7269\u7d20\u5316\u6297\u9ad4\u65bc\u86cb\u767d\u8cea\u7d14\u5316\u3001\u86cb\u767d\u8cea\u6aa2\u6e2c\uff0c\u6216\u56fa\u5b9a\u4e4b\u61c9\u7528\u3002<\/p>\n\n\n\n\n\n\n\n\n\n
Alternate Name<\/strong><\/td>\nN-[6-(Biotinamido)hexyl]-3\u2019-(2\u2019-pyridyldithio)-propionamide; Biotin-HPDP, pyridyldithiol-biotin, pyridyldisulfide-biotin<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nWhite to Off-white Solid<\/td>\n<\/tr>\n
CAS #<\/strong><\/td>\n129179-83-5<\/td>\n<\/tr>\n
Molecular Formula<\/strong><\/td>\nC\u2082\u2084H\u2083\u2087N\u2085O\u2083S\u2083<\/td>\n<\/tr>\n
Molecular Weight<\/strong><\/td>\n539.78<\/td>\n<\/tr>\n
Purity<\/strong><\/td>\n\u226590% by NMR<\/td>\n<\/tr>\n
Solubility<\/strong><\/td>\nDMSO or DMF<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u86cb\u767d\u8cea\u5b9a\u91cf\u5546\u54c1<\/span> Coomassie blue\u86cb\u767d\u8cea\u5b9a\u91cf\u5206\u6790\u5957\u7d44<\/a> | BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2(\u4f4e\u6fc3\u5ea610-600ug\/ml)| 5\uff05\u8010\u6d17\u6ecc <\/a>| BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2 (\u9084\u539f\u5291\u517c\u5bb9)<\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Crosslinking\u8a66\u5291\u7cfb\u5217\u5546\u54c1 Biotin-HPDP \u00a0N- [6-\uff08\u751f\u7269\u7d20\u9170\u80fa\uff09\u5df1\u57fa]-3&#824 […]<\/p>\n","protected":false},"author":3,"featured_media":5468,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[134],"tags":[3404],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12465"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=12465"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12465\/revisions"}],"predecessor-version":[{"id":12476,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/12465\/revisions\/12476"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/5468"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=12465"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=12465"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=12465"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}