{"id":11776,"date":"2017-07-11T12:08:28","date_gmt":"2017-07-11T04:08:28","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=11776"},"modified":"2022-11-08T16:24:37","modified_gmt":"2022-11-08T08:24:37","slug":"caspase-3%e5%91%88%e8%89%b2%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-caspase-3-colorimetric-assay-kit%e8%b2%a8%e8%99%9fk106","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=11776","title":{"rendered":"Caspase-3\u5448\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 |Caspase-3 Colorimetric Assay Kit\u8ca8\u865fK106-100 (Abcam ab39401)"},"content":{"rendered":"

Caspase-3\u5448\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 \u00a0| <\/strong>\u8ca8\u865f<\/strong>K106<\/a>\u00a0\u00a0(Abcam ab39401)\u00a0<\/span><\/p>\n

Convenient, Highly-Sensitive Assay<\/em><\/strong><\/p>\n

Caspase<\/strong>\u7cfb\u5217\u7522\u54c1\u4ecb\u7d39<\/strong>\uff1a<\/strong><\/p>\n

\u7576\u7d30\u80de\u63a5\u6536\u51cb\u4ea1\u8a0a\u865f\u5f8c\uff0c\u5c07\u4e0d\u6d3b\u5316\u614b\u7684pro-caspase\u8f49\u8b8a\u70ba\u6d3b\u5316\u614bcaspase\uff0c\u5f15\u767ccaspase cascade\u9020\u6210\u7d30\u80de\u51cb\u4ea1\u3002\u4e3b\u8981\u7d93\u7531Caspases 8\u30019\u300112\u8abf\u63a7\u7d30\u80de\u51cb\u4ea1\u7684\u5916\u5728\u8def\u5f91(extrinsic pathway)\u3001\u5167\u5728(intrinsic pathway)\u3001\u4ee5\u53caER stress\uff0c\u6700\u5f8c\u9020\u6210Caspases 3\u7684\u6d3b\u5316\u3002Biovision\u63d0\u4f9b\u5b8c\u6574Caspase\u76f8\u95dc\u5206\u6790\u5957\u7d44\u3001inhibitor\u3001substrate\u7b49\u5404\u985e\u5546\u54c1\u3002Caspase \u5206\u6790\u5957\u7d44\uff0fSubstrate: \u5f9eCaspase1-13\u3001Pan Caspase\u7686\u80fd\u5206\u6790\u3002\u5206\u6790\u5957\u7d44\u90e8\u5206\u6709\u5316\u5b78\u5448\u8272\u53cd\u61c9\u53ca\u87a2\u5149\u53ef\u9078\u64c7\uff1bSubstrate\u90e8\u5206\u6709\u5316\u5b78\u5448\u8272\u7269\u8ceapNA\uff08\u03bb= 400 nm\uff09\u8207\u87a2\u5149\u5448\u8272\u7269\u8ceaAFC\uff08Ex\/Em\uff1d400\/505 nm\uff09\u5169\u7a2e\u53ef\u61c9\u7528\u3002Caspase Inhibitors: Caspase \u6291\u5236\u5291\u4e3b\u8981\u7d50\u5408Caspase\u6d3b\u5316\u4f4d\uff0c\u9032\u800c\u9054\u5230\u8a0a\u606f\u6291\u5236\u6548\u679c\u3002fluoromethylketone\uff08FMK\uff09\u985e\u7684\u6291\u5236\u5291\uff0c\u70ba\u4e3b\u8981\u7814\u7a76\u6297\u7d30\u80de\u51cb\u4ea1\u7684\u5de5\u5177\u4e4b\u4e00\u3002\u65b0\u4e00\u4ee3Caspase\u6291\u5236\u5291Q-(XXXX)-OPh\uff0c\u56e0\u5176\u7d30\u80de\u6bd2\u6027\u8f03\u4f4e\uff0c\u4e14\u7a69\u5b9a\u6027\u8f03FMK\u985e\u6291\u5236\u5291\u9ad8\uff0c\u8f03\u9069\u5408\u5728in vivo\u5be6\u9a57\u4e2d\u4f7f\u7528\u3002<\/p>\n

\"\"\"\"<\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1aICE-family proteases\/caspases\u00a0<\/strong>ICE\u5bb6\u65cf\u86cb\u767d\u9176\/\u534a\u80f1\u5929\u51ac\u9176\u7684\u6fc0\u6d3b\u555f\u52d5\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u51cb\u4ea1\u3002 Caspase-3 \/ CPP32\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u4fbf\u65b9\u4fbf\u7684\u65b9\u6cd5\u6e2c\u5b9aDEVD\u8b58\u5225\u5e8f\u5217\uff0c\u6e2c\u91cfCaspase-3\u534a\u80f1\u5929\u51ac\u9176\u4e4b\u6d3b\u6027\u3002 \u8a72\u6e2c\u5b9a\u6cd5\u57fa\u65bc\u5f9eDEVD-pNA\u6a19\u8a18\u5e95\u7269\uff0c\u5207\u5272\u5f8c\uff08pNA\uff09\u4ee5400\u6216405nm\u6ce2\u6bb5\u4e0b\uff0c\u6aa2\u6e2c\u91cf\u5316\u4e4bpNA\u767c\u5149\u3002 \u6bd4\u8f03\u4f86\u81ea\u51cb\u4ea1\u6a23\u54c1\u7684pNA\u7684\u5438\u5149\u5ea6\u8207\u672a\u8a98\u5c0e\u7684\u5c0d\u7167\u53ef\u4ee5\u78ba\u5b9aCPP32\u6d3b\u6027\u7684\u500d\u6578\u589e\u52a0\u3002\u9069\u7528\u65bc\u7d30\u80de\u6216\u7d44\u7e54\u4e4b\u5be6\u9a57 (not for plasma)\uff0cSubstrate\u6aa2\u6e2c\u4e4b\u9748\u654f\u5ea6\u70ba>1uM\u3002\u8a72\u8a66\u5291\u76d2\u5c07\u5f9e\u6a23\u54c1\u4e2d\u6aa2\u6e2c\u6d3b\u6027Caspase-3 (procasepase-3\uff08\u7121\u6d3b\u6027\uff09\u86cb\u767d\u6c34\u89e3\u52a0\u5de5\u6210\u6d3b\u6027Caspase-3)\u3002<\/p>\n\n\n\n\n\n\n\n
Detection Method<\/strong><\/td>\nAbsorbance (400 or 405 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nDetect early\/middle stages of apoptosis; differentiate apoptosis from necrosis.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple one-step procedure; takes 1-2 hours
\n\u2022 Fast and convenient
\n\u2022 Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Cell Lysis buffer
\n\u2022 2X Reaction Buffer
\n\u2022 DEVD-pNA (4 mM)
\n\u2022 DTT (1 M)
\n\u2022 Dilution Buffer<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7522\u54c1\u4f7f\u7528\u6ce8\u610f\u4e8b\u9805:<\/strong><\/p>\n

    \n
  1. \u8acb\u78ba\u5be6\u5c07\u7d30\u80de\u5b8c\u5168\u6eb6\u89e3\uff0c\u5c07\u7d30\u80de\u6c89\u6fb1\u91cd\u61f8\u65bc\u88c2\u89e3\u7de9\u885d\u6db2\u4e2d\u4e26\u6309\u7167\u6578\u64da\u8868\u4e2d\u6240\u8ff0\u9032\u884c\u53cd\u61c9\u3002<\/li>\n
  2. \u51cb\u4ea1\u8a98\u5c0e\u53ef\u4f7f\u7528\u7528 1-5 x 106<\/sup> cells) 20 hour exposure to 2 uM Camptothecin \u6216\u800510 ng\/mL anti-Fas Ab\u505a\u70baPositive control<\/li>\n
  3. \u4f7f\u7528\u65b0\u9bae\u89e3\u51cd\u7684DTT\u52a0\u65bc\u7d30\u80de\u88c2\u89e3\u7de9\u885d\u6db2\u4e2d\u4f7f\u7528\u3002Aliquot DTT so that you have enough to perform the desired number of assays. Store at -20\u00b0C.<\/li>\n
  4. \u8a72\u7522\u54c1\u53ef\u6aa2\u6e2c\u86cb\u767d\u6c34\u89e3\u6d3b\u6027\u3002\u86cb\u767d\u9176\u6291\u5236\u5291\u6703\u5e72\u64feCapase\u6e2c\u5b9a\u3002 Caspase 3\u662f\u4e00\u7a2e\u534a\u80f1\u6c28\u9178\u86cb\u767d\u9176\uff0c\u53ef\u88ab\u5927\u591a\u6578\u86cb\u767d\u9176\u6291\u5236\u5291\u6df7\u5408\u7269\u6291\u5236\u3002 \u8a72\u6e2c\u5b9a\u57fa\u65bcCaspase-3\u5c0d\u5e95\u7269DEVD-pNa\u7684\u50ac\u5316\u88c2\u89e3\u3002 \u56e0\u6b64\uff0c\u534a\u80f1\u5929\u51ac\u91763\u7684\u6291\u5236\u6703\u5e72\u64fe\u6e2c\u91cf\u3002<\/li>\n
  5. \u7522\u54c1\u9069\u7528\u7684\u6a23\u54c1\u70ba cell \u53ca tissue lysates\uff0c\u4e0d\u9069\u7528\u65bcplasma \u6a23\u54c1 (\u8840\u5c0f\u677f\u53ef\u80fd\u6703\u5e72\u64fe\u53cd\u61c9)\u3002<\/li>\n<\/ol>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Caspase-3\u5448\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 \u00a0| \u8ca8\u865fK106\u00a0\u00a0(Abcam ab39401)\u00a0 Convenient […]<\/p>\n","protected":false},"author":3,"featured_media":10325,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[128],"tags":[3481],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11776"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=11776"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11776\/revisions"}],"predecessor-version":[{"id":35859,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11776\/revisions\/35859"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10325"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=11776"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=11776"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=11776"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}