{"id":11602,"date":"2017-07-05T11:46:54","date_gmt":"2017-07-05T03:46:54","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=11602"},"modified":"2025-03-21T15:02:12","modified_gmt":"2025-03-21T07:02:12","slug":"%e6%af%9b%e5%9b%8a%e7%9c%9f%e7%9a%ae%e4%b9%b3%e9%a0%ad%e7%b4%b0%e8%83%9e-htert-immortalized-hair-follicle-dermal-papilla-cells-htert%e8%b2%a8%e8%99%9ft0501","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=11602","title":{"rendered":"\u6bdb\u56ca\u771f\u76ae\u4e73\u982d\u7d30\u80de – hTERT | Immortalized Hair Follicle Dermal Papilla Cells – hTERT\u8ca8\u865fT0501"},"content":{"rendered":"

HOT!!<\/strong><\/span>\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/p>\n

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)<\/strong><\/p>\n

\"\"<\/p>\n

\u6c38\u751f\u5316\u6bdb\u56ca\u771f\u76ae\u4e73\u982d\u7d30\u80de – hTERT<\/a> <\/strong><\/span>(<\/strong>\u8ca8\u865f<\/strong>T0501<\/strong>)\u00a0<\/strong>Immortalized Hair Follicle Dermal Papilla Cells – hTERT<\/p>\n

\u00a0<\/strong>\u4eba\u6bdb\u56ca\u771f\u76ae\u4e73\u982d\u7d30\u80de\u662f\u5f9e\u982d\u76ae\u6bdb\u56ca\u7d30\u80de\u5916\u57fa\u8cea\u4e2d\u5206\u96e2\u51fa\u7684\u982d\u9aee\u4e73\u982d\u5206\u96e2\u7684\u9ad8\u6d3b\u6027\u9593\u5145\u8cea\u7d30\u80de\u3002 \u771f\u76ae\u4e73\u982d\u7d30\u80de\u901a\u904e\u6d89\u53ca\u6bdb\u56ca\u7d30\u80de\u7684\u4e0a\u76ae – \u9593\u8cea\u76f8\u4e92\u4f5c\u7528\u5728\u63a7\u5236\u6bdb\u9aee\u751f\u9577\u5468\u671f\u548c\u7522\u751f\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002 \u5b83\u5011\u7684\u5b58\u6d3b\u7531\u4fe1\u865f\u8f49\u5c0e\u9014\u5f91\u5982ERK\u548cAkt\u8abf\u7bc0\u3002 \u9019\u4e9b\u7d30\u80de\u53ef\u7528\u65bc\u767c\u80b2\u548c\u8a55\u4f30\u6bdb\u9aee\u751f\u9577\u7522\u7269\u4e26\u9451\u5b9a\u6bdb\u56ca\u5167\u7684\u7d30\u80de\u7fa4\u3002 \u6b64\u5916\uff0c\u5b83\u5011\u53ef\u7528\u65bc\u96c4\u6fc0\u7d20\u963b\u65b7\u8a66\u5291\u7684\u9ad4\u5916\u7be9\u9078\uff0c\u56e0\u70ba\u5b83\u5011\u5177\u6709\u96c4\u6fc0\u7d20\u53d7\u9ad4\u3002<\/p>\n

\"\"<\/p>\n

Immortalized Hair Follicle Dermal Papilla Cells – hTERT\u6bdb\u56ca\u771f\u76ae\u4e73\u982d\u7d30\u80de<\/strong>Adherent \/ Multipolar | \u4e0d\u6747\u65b9\u6cd5: hTERT | \u4f86\u6e90Hair Follicle (Female\u6350\u8d08\u7a2e\u65cf\u767d\u7a2e\u4eba) \u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>) \u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2PriGrowI II(\u8ca8\u865f\u00a0TM003)\uff0c\u80ce\u725b\u8840\u6e05(Cat. No. TM999<\/a>)\u6700\u7d42\u6fc3\u5ea6\u70ba10%\u4e26\u586b\u52a01% Penicillin\/Streptomycin \u30021%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C\u7d30\u80de\u7279\u6027<\/strong>:<\/strong> 40-42 hours\u3002\u578b\u614b\u70baMultipolar.\u3002\u5efa\u8b70\u7e7c\u4ee3\u7d30\u80de\u6578\u70ba6,000 – 10,000 cells per cm^2\u3002\u7d30\u80de\u57f9\u990a\u6db2\u66f4\u63db\u5927\u7d042-3\u5929\uff1b\u7d30\u80de\u7e7c\u4ee3\u70ba\u80f0\u86cb\u767d\u9176\u6eb6\u6db20.25% trypsin(TM050) \u7684\u4e0b37\u2103\u4e0bincubator 3-5\u5206\u9418\uff0c\u76f4\u5230\u7d30\u80de\u74b0\u7e5e\uff0c\u5728\u986f\u5fae\u93e1\u4e0b\u4e0d\u6642\u76e3\u6e2c\u3002\u7d30\u80de\u5916\u57fa\u8ceaCoating dish\uff0c\u7528PBS\u7a00\u91cbG422\uff081\uff1a1\uff09\u4e26\u5857\u8986\u66f4\u5c11\u7684\u6642\u9593\u3002\u81a0\u539f\u86cb\u767d\u542b\u91cf\u8f03\u9ad8\uff0c\u5f9e\u800c\u4f7f\u8207\u7d30\u80de\u7684\u7d50\u5408\u66f4\u5f37\u3002\u53ef\u4ee5\u5617\u8a66\u6e1b\u5c11incubator\u6642\u9593\u4ee5\u53ca\u5857\u8986\u66f4\u8584\u7684\u5c64\u3002<\/p>\n

\"\"<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

HOT!!\u00a0\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c\u00a0|\u00a0DAPI\u67d3\u6838\u00a0|\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291\u00a0|\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291\u00a0|\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49 […]<\/p>\n","protected":false},"author":3,"featured_media":15022,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[453],"tags":[3513],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11602"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=11602"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11602\/revisions"}],"predecessor-version":[{"id":50858,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/11602\/revisions\/50858"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/15022"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=11602"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=11602"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=11602"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}