{"id":10851,"date":"2017-06-20T16:07:28","date_gmt":"2017-06-20T08:07:28","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=10851"},"modified":"2022-07-22T09:50:49","modified_gmt":"2022-07-22T01:50:49","slug":"adam23%e6%85%a2%e7%97%85%e6%af%92-sirnashrnarnai-lentivirus-human-pooled-%e8%b2%a8%e8%99%9f-iv000540","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=10851","title":{"rendered":"ADAM23\u6162\u75c5\u6bd2 | siRNA\/shRNA\/RNAi Lentivirus (Human) pooled \u8ca8\u865f iV000540"},"content":{"rendered":"

ADAM23 siRNA\/shRNA\/RNAi Lentivirus (Human) pooled <\/strong>\u6162\u75c5\u6bd2<\/strong> | <\/strong>\u8ca8\u865f<\/strong>\u00a0 iV000540<\/strong><\/a><\/span><\/p>\n

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ADAM23\u5373\u7528\u578b\u75c5\u6bd2\u6db22×200\u00b5l >107<\/sup>\u00a0IU\/ml<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Species<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Accession Number<\/strong><\/td>\nNM_003812<\/a><\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npiLenti-siRNA-GFP<\/a><\/td>\n<\/tr>\n
Target Sequence<\/strong><\/td>\nSequence available upon placing order<\/td>\n<\/tr>\n
User Manual<\/strong><\/td>\n<\/td>\n<\/tr>\n
Mammalian Selection<\/strong><\/td>\nPuromycin<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nVirus<\/td>\n<\/tr>\n
Pictures<\/strong><\/td>\n<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLiquid<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\nLentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80\u00b0C in small aliquots to avoid repeated freeze-thaw cycles.<\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npLenti-U6-sgRNA-SFFV-Cas9-2A-Puro<\/a><\/td>\n<\/tr>\n
Cas9 Promoter<\/strong><\/td>\nCas9 Nuclease is under the control of the SFFV promoter which should work for a vast majority of cells, except ES cells or iPS cells. If necessary, the SFFV promoter can be replaced with EF1a promoter as a custom cloning service. Please inquire for pricing.<\/td>\n<\/tr>\n
Target Sequence<\/strong><\/td>\nTarget 3: Sequence available upon placing order<\/td>\n<\/tr>\n
Sequencing Primers<\/strong><\/td>\nU6 Forward Primer: 5\u2019–TACGTCCAAGGTCGGGCAGGAAGA–3\u2019<\/td>\n<\/tr>\n
Bacterial Selection<\/strong><\/td>\nAmpicillin<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nPlasmid<\/td>\n<\/tr>\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Organism<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
SourceOrgan<\/strong><\/td>\nKidney<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nPolygonal<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nHEK293T cells stably expressing Cas9<\/td>\n<\/tr>\n
Markers<\/strong><\/td>\nPuromycin<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1<\/span> CRISPR\u6aa2\u6e2c\u5957\u7d44<\/a> | CRISPR\u57fa\u56e0\u5254\u9664\u7d30\u80de\u682a<\/a> | CASPASE9 (D10A) NLS<\/a><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

ADAM23 siRNA\/shRNA\/RNAi Lentivirus (Human) pooled \u6162\u75c5\u6bd2 | […]<\/p>\n","protected":false},"author":3,"featured_media":10852,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[456],"tags":[3627],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10851"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10851"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10851\/revisions"}],"predecessor-version":[{"id":10854,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10851\/revisions\/10854"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10852"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10851"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10851"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10851"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}