{"id":10801,"date":"2017-06-19T12:01:04","date_gmt":"2017-06-19T04:01:04","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=10801"},"modified":"2022-07-22T09:51:43","modified_gmt":"2022-07-22T01:51:43","slug":"lysis-buffer-%e7%b4%b0%e8%83%9e%e7%b5%84%e7%b9%94%e8%a3%82%e8%a7%a3%e7%b7%a9%e8%a1%9d%e6%b6%b2for-cells-and-tissue","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=10801","title":{"rendered":"Lysis Buffer | \u7d30\u80de\u7d44\u7e54\u88c2\u89e3\u7de9\u885d\u6db2for Cells and Tissue"},"content":{"rendered":"<p><span style=\"color: #3366ff;\"><strong><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10801\">Lysis Buffer for Cells and Tissue<\/a> <\/strong><\/span><\/p>\n<p><strong>\u5546\u54c1:<\/strong> \u88c2\u89e3\u7de9\u885d\u6db2\u7528\u65bc\u5728\u975e\u8b8a\u6027\u7de9\u885d\u6db2\u689d\u4ef6\u4e0b\u88c2\u89e3\u7d30\u80de\uff0c\u7531\u8868\u9762\u6d3b\u6027\u5291\uff0c\u86cb\u767d\u9176\u6291\u88fd\u5291\u7b49\u7d44\u6210\uff0c\u4e3b\u8981\u7528\u65bc\u5f9e\u7d30\u80de\u548c\u7d44\u7e54\u4e2d\u63d0\u53d6\u86cb\u767d\u8cea\u3002 \u50b3\u7d71\u7684\u88c2\u89e3\u7de9\u885d\u6db2\u512a\u5316\u4e86\u56db\u7a2e\u88c2\u89e3\u7de9\u885d\u6db2\u3002 \u5b83\u5011\u9069\u7528\u65bc\u7d30\u80de\u819c\uff0c\u7d30\u80de\u8cea\u548c\u7d30\u80de\u6838\u7b49\u7684\u88c2\u89e3\uff0c\u6709\u52a9\u65bc\u91cb\u653e\u86cb\u767d\u8cea\uff0c\u6709\u5229\u65bc\u665a\u671f\u63d0\u53d6\uff0cELISA\uff0cWestern Blot\u7b49\u3002\u70ba\u4e86\u907f\u514d\u86cb\u767d\u8cea\u964d\u89e3\uff0c\u5efa\u8b70\u5728\u4f7f\u7528\u524d\u5c071-2mM PMSF\u52a0\u5165\u5230Lysis Buffer\u4e2d\u3002\u8acb\u907f\u514d\u51cd\u878d\u5faa\u74b0\uff0c\u5efa\u8b70\u5728\u63a5\u6536\u6642\u7b49\u5206\u3002\u5728\u51b0\u4e0a\u5efa\u8b70\u88c2\u89e3\u904e\u7a0b\u3002\u57284\u2103\u9577\u6642\u9593\u5132\u5b58\u5f8c\uff0c\u6eb6\u89e3\u7de9\u885d\u6db2\u4e2d\u53ef\u80fd\u6703\u6c89\u6fb1\uff0c\u4f46\u5728\u5ba4\u6eab\u653e\u7f6e\u5f8c\u6703\u6eb6\u89e3\uff0c\u4e0d\u6703\u5f71\u97ff\u5be6\u9a57\u7684\u7d50\u679c\u3002\u6839\u64da\u6a23\u54c1\u7684\u7279\u9ede\u548c\u5be6\u9a57\u76ee\u7684\u9078\u64c7\u88c2\u89e3\u7de9\u885d\u6db2\u3002 \u4ee5\u4e0b\u70ba\u88c2\u89e3\u7de9\u885d\u6db2\u7684\u7a2e\u985e\u548c\u6700\u4f73\u5be6\u9a57\u689d\u4ef6\u3002<\/p>\n<p><strong>Lysis Buffer for Cells and Tissue<\/strong><\/p>\n<table>\n<tbody>\n<tr>\n<td>Name<\/td>\n<td>Applications<\/td>\n<\/tr>\n<tr>\n<td>Lysis Buffer 1<\/td>\n<td>Membrane proteins including surface proteins, receptors<\/td>\n<\/tr>\n<tr>\n<td>Lysis Buffer 2<\/td>\n<td>Cytoplasmic proteins, structural proteins, etc.<\/td>\n<\/tr>\n<tr>\n<td>Lysis Buffer 3<\/td>\n<td>Cytoplasmic proteins, nuclear matrix proteins, membrane proteins, etc.<\/td>\n<\/tr>\n<tr>\n<td>Lysis Buffer 4<\/td>\n<td>Nuclear matrix proteins, endosome and other organelles protein<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<table>\n<tbody>\n<tr>\n<td>REAGENT<\/td>\n<td>Quantity<\/td>\n<td>Usage and Dosage<\/td>\n<\/tr>\n<tr>\n<td>Lysis Buffer<\/td>\n<td>20ml(1\u00d7)<\/td>\n<td>10 7 cells\/50mg tissues by 1 ml lysis buffer.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u00a0<\/strong><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Lysis Buffer for Cells and Tissue \u5546\u54c1: \u88c2\u89e3\u7de9\u885d\u6db2\u7528\u65bc\u5728\u975e\u8b8a\u6027\u7de9\u885d\u6db2\u689d\u4ef6\u4e0b [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":10802,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[89,134,179],"tags":[3633],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10801"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10801"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10801\/revisions"}],"predecessor-version":[{"id":10804,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10801\/revisions\/10804"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10802"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10801"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10801"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10801"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}