{"id":10796,"date":"2017-06-15T16:42:18","date_gmt":"2017-06-15T08:42:18","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=10796"},"modified":"2022-07-22T09:52:19","modified_gmt":"2022-07-22T01:52:19","slug":"sumo-protease-1c-site-specific-proteases-%e8%b2%a8%e8%99%9fg696","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=10796","title":{"rendered":"SUMO Protease 1C |Site-Specific Proteases  \u8ca8\u865fG696"},"content":{"rendered":"<p><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10796\"><strong>Site-Specific Proteases <\/strong><strong>SUMO Protease 1C<\/strong><strong> | <\/strong><strong>\u8ca8\u865f<\/strong><\/a><\/span><strong><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10796\">\u00a0\u00a0 G696\u00a0\u00a0\u00a0\u00a0 <\/a><\/span>\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong><\/p>\n<p>\u86cb\u767d\u8cea\u7d14\u5316\u70ba\u5f9e\u76ee\u6a19\u86cb\u767d\u8cea\u8207\u751f\u7269\u5206\u5b50\u6df7\u5408\u7269\u5206\u96e2\u7684\u904e\u7a0b\u3002\u89aa\u548c\u7d14\u5316\u65b9\u5f0f\u4f9d\u8cf4\u65bc immobilized ligands\u56fa\u5b9a\u5316\u914d\u9ad4\uff08\u6a39\u8102\uff09\u548c\u517c\u5bb9\u6a19\u7c64\uff08\u9776\u86cb\u767d\u6216\u8207\u9776\u86cb\u767d\u76f8\u95dc\uff09\u4e4b\u9593\u7684\u9ad8\u7d50\u5408\u89aa\u548c\u529b\u3002 abmgood\u63d0\u4f9b\u4e86\u5404\u7a2e\u89aa\u548c\u529b\u6a39\u8102\uff0c\u5176\u4e2d\u5305\u62ec\u5206\u5225\u7528\u65bc\u7d14\u5316His\u548cGST\u6a19\u8a18\u86cb\u767d\u7684Nickel\u93b3\u548cGlutathione agarose beads\u7a40\u80f1\u7518\u80bd\u74ca\u8102\u7cd6\u73e0\uff0c\u4ee5\u53ca\u7528\u65bc\u7d14\u5316IgG\u6297\u9ad4\u7684protein A\/G beads\u86cb\u767dA \/ G\u73e0\u3002 abmgood\u9084\u63d0\u4f9b\u9ad8\u8cea\u91cf\u7684\u86cb\u767d\u9176\uff0c\u7528\u65bc\u96a8\u5f8c\u53bb\u9664\u89aa\u548c\u6a19\u7c64\uff0c\u4fbf\u65bc\u8cfc\u8cb7\u86cb\u767d\u8cea\u7d14\u5316\u6240\u9700\u7684\u8a66\u5291\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abmgood.com\/ProteinPurification\/images\/SUMO-protease-Western-Blot.png\" alt=\"\" width=\"229\" height=\"382\" \/><\/p>\n<p><strong>SUMO Protease 1C<\/strong><strong>\u7522\u54c1\u4ecb\u7d39<\/strong><strong>:<\/strong> \u4e3b\u8981\u61c9\u7528\u65bc\u91cd\u7d44\u86cb\u767d\u4e2d\u53bb\u9664SUMO fusion tag\u3002\u901a\u904e\u4f7f\u7528polyhistidine tag\u805a\u7d44\u6c28\u9178\u6a19\u7c64affinity chromatography\u89aa\u548c\u5c64\u6790\uff0c\u5f9e\u5927\u8178\u687f\u83cc\u7d14\u5316\u4e4b\u91cd\u7d44\u4ebaSUMO\u86cb\u767d\u91761C\u3002 \u86cb\u767d\u9176\u4ee5\u9ad8\u5ea6\u7279\u7570\u6027\u7684\u65b9\u5f0f\u5f9e\u91cd\u7d44\u878d\u5408\u86cb\u767d\u4e2d\u5207\u5272SUMO\u3002\u6d88\u5316\u5f8c\uff0c\u4f7f\u7528\u5176\u5728\u86cb\u767d\u9176N\u672b\u7aef\u878d\u5408\u7684\u805a\u7d44\u6c28\u9178\u6a19\u7c64\uff0c\u901a\u904e\u89aa\u548c\u5c64\u6790\u5bb9\u6613\u5730\u5f9e\u5207\u5272\u53cd\u61c9\u4e2d\u9664\u53bbSUMO\u86cb\u767d\u9176\u3002\u4ebaSUMO\u86cb\u767d\u91761C\u91cd\u7d44\u5728\u7de9\u6c96\u6c34\u6eb6\u6db2\u4e2d\uff0c\uff084\u2103\u81f337\u2103\uff09\u5177\u9ad8\u6d3b\u6027\u88c2\u89e3\uff0c\u53ca\u7279\u7570\u6027\u5207\u5272\uff0c\u7121\u975e\u7279\u7570\u6027\u86cb\u767d\u6c34\u89e3\u4e4b\u7279\u6027\u3002SUMO\u86cb\u767d\u9176\uff080.5U \/\u03bcl\uff09\uff1a4\u00d750\u03bcl\uff0c100\u03bcl\uff0c\u7e3d\u5171200\u03bcl***\u4e00\u500b\u55ae\u4f4d\u7684SUMO\u86cb\u767d\u9176\u572830\u2103\u57281\u5c0f\u6642\u5167\u5207\u5272\u226585\uff05\u76842ug\u5e95\u7269\u86cb\u767d\u8cea***<\/p>\n<p><span style=\"color: #3366ff;\"><strong><span style=\"color: #ff0000;\">\u5176\u5b83\u5546\u54c1<\/span>\u00a0<\/strong><strong><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10749\"><strong><u>AKT2<\/u><\/strong><u>\u00a0<\/u><strong><u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><u>\u00a0<\/u><\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10752\"><strong><u>EGFR<\/u><\/strong><strong><u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><u>\u00a0<\/u><\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10756\"><strong><u>HER2<\/u><\/strong><strong><u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10763&amp;preview=true\"><strong><u>LIMK1<\/u><\/strong><strong><u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10766\"><strong><u>MEK1 (EE)<\/u><\/strong><u>\u00a0<\/u><strong><u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><u>\u00a0<\/u><\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10771\"><u>\u00a0<\/u><strong><u>ROCK2<\/u><\/strong><strong><u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10776\"><strong><u>ROS1 (G2032R)<\/u><\/strong><strong><u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a><\/strong><\/span><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Site-Specific Proteases SUMO Protease 1C | \u8ca8\u865f\u00a0\u00a0 G696\u00a0\u00a0\u00a0 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":10750,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[89],"tags":[3634],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10796"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10796"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10796\/revisions"}],"predecessor-version":[{"id":10798,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10796\/revisions\/10798"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10750"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10796"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10796"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10796"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}