{"id":10086,"date":"2017-05-10T14:52:38","date_gmt":"2017-05-10T06:52:38","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=10086"},"modified":"2022-07-22T10:29:30","modified_gmt":"2022-07-22T02:29:30","slug":"10086","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=10086","title":{"rendered":"\u7e3d\u8f49\u9304\u7d44cDNA\u5408\u6210\u8a66\u5291\u76d2 | Total-Transcriptome cDNA Synthesis Kit\u8ca8\u865fG905"},"content":{"rendered":"<p><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10086\"><strong>\u7e3d\u8f49\u9304\u7d44cDNA\u5408\u6210\u8a66\u5291\u76d2<\/strong><strong> | <\/strong><strong>\u8ca8\u865f<\/strong><strong>G905 100\u500b\u53cd\u61c9<\/strong><\/a><\/p>\n<p>Abmgood\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217RNA\u548cDNA\u7d14\u5316\u8a66\u5291\u76d2\uff0c\u53ef\u4ee5\u7522\u751f\u9ad8\u8cea\u91cfRNA\u548cDNA\uff0c\u9069\u5408\u65bc\u4e0b\u6e38\u5982sequencing\u5b9a\u5e8f\u3001PCR\u3001\u9650\u5236\u6027\u5207\u5272\u8207\u5206\u5b50\u9023\u63a5\u3002\u7522\u54c1\u8a2d\u8a08\u539f\u7406\u70basilica spin column\u65cb\u8f49\u67f1\u7d14\u5316\u65b9\u6cd5\uff0c\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u5feb\u901f\uff0c\u66ff\u4ee3\u50b3\u7d71\uff08\u4e59\u9187\u6c89\u6fb1\uff09\u548c\u4e0d\u9700\u8981\u6bd2\u6027\u915a\/\u6c2f\u4eff\u8403\u53d6\u6216\u7e41\u7463\u7684CsCl\u68af\u5ea6\uff0cLiCl\u6216\u4e59\u9187\u6c89\u6fb1\u6280\u8853\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abmgood.com\/PCR\/images\/Total-Transcriptome_cDNA_Synthesis_kit-G904-G905.jpg\" alt=\"\" width=\"511\" height=\"381\" \/><\/p>\n<p><strong>\u7522\u54c1\u4ecb\u7d39: <\/strong>\u00a0abm\u7684\u7e3d\u8f49\u9304\u7d44cDNA\u5408\u6210\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u5728\u6a23\u54c1\uff08\u5982mRNA\uff0cmiRNA\u548clncRNA\uff09\u4e2d\u5f9e\u6240\u6709\u4e0d\u540c\u7a2e\u985e\u7684RNA\u7522\u751f\u9ad8\u8cea\u91cfcDNA\u6240\u9700\u7684\u6240\u6709\u8a66\u5291\u3002\u9019\u53ef\u4ee5\u901a\u904e\u4f7f\u7528\u805a\uff08A\uff09\u805a\u5408\u9176\u76843&#8217;\u591a\u805a\uff08A\uff09RNA\u7684RNA\u4f86\u5be6\u73fe\u3002 Oligo d\uff08T\uff09\u929c\u63a5\u5b50\u548c\u96a8\u6a5f\u5f15\u7269\u7136\u5f8c\u9000\u706b\u5230RNA\u6a21\u677f\u4e0a\u4e26\u4fc3\u9032\u9006\u8f49\u9304\u3002 OneScript\u00aePlus\u9006\u8f49\u9304\u9176\u662f\u4e00\u7a2e\u65b0\u578b\u7684\u91cd\u7d44\u9006\u8f49\u9304\u9176\uff0c\u5176\u5728\u5177\u6709\u4e8c\u7d1a\u7d50\u69cb\u548c\u9ad8GC\u542b\u91cf\u7684RNA\u6a21\u677f\u4e2d\u5728\u7b2c\u4e00\u93c8cDNA\u5408\u6210\u4e2d\u8868\u73fe\u51fa\u66f4\u9ad8\u7684\u6548\u7387\u3002 OneScript\u00aePlus\u9006\u8f49\u9304\u9176\u8a2d\u8a08\u7528\u65bc\u5728\u9ad8\u6eab\uff0850\u00b0C\u81f355\u00b0C\uff09\u4e0b\u9032\u884c;\u6709\u52a9\u65bc\u6d88\u9664\u8207\u5bcc\u542bGC\u7684RNA\u6a21\u677f\u76f8\u95dc\u7684\u4e8c\u7d1a\u7d50\u69cb\u3002\u91cd\u7d44RNaseOFF\u6838\u7cd6\u6838\u9178\u9176\u6291\u88fd\u5291\u6709\u6548\u4fdd\u8b77RNA\u6a21\u677f\u4e0d\u88ab\u964d\u89e3\u3002\u6b64\u5916\uff0c\u8a66\u5291\u76d2\u4e2d\u5305\u542b\u7684\u901a\u75283&#8217;\u53cd\u5411\u5f15\u7269\u53ef\u4ee5\u8207\u4e0b\u6e38qPCR \/ PCR\u64f4\u589e\u4e2d\u7684\u4efb\u4f55\u7279\u7570\u6027\u6b63\u5411\u5f15\u7269\u5076\u806f\u3002<strong>\u7522\u54c1\u7279\u9ede: <\/strong>\u53ef\u61c9\u7528\u65bc\u4eba\u6216\u5c0f\u9f20RNA\u6a23\u54c1\u4e2d\u7684\u6240\u6709RNA; \u91cd\u73fe\u6027\u597d\uff0c\u7522\u91cf\u9ad8\uff1b\u5b8c\u6574\uff0c\u5168\u9762cDNA\u5408\u6210\u8a66\u5291\u76d2\u3002\u5305\u62ecoligo dT Adaptor\u548c\u9ad8\u6548\u7387\u7279\u6b8a\u53cd\u8f49\u9304primer\u8a2d\u8a08\u3002mRNA\uff0cmiRNA\u548clncRNA\u7814\u7a76\u7684\u967d\u6027\u5c0d\u7167\u7d44\u3002<\/p>\n<p><span style=\"color: #ff0000;\">\u76f8\u95dc\u5546\u54c1 <\/span><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10106\">\u7d44\u7e54RNA Mini Kit w\/DNase<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10103\"><u>Second-Strand cDNA<\/u><u>\u5408\u6210\u8a66\u5291\u76d2<\/u><\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10098\"><u>\u57fa\u56e0\u7d44<\/u><u>DNA<\/u><u>\u53bb\u9664\u8a66\u5291\u76d2<\/u><\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10094\"><u>RNaseOFF<\/u><u>\u6838\u7cd6\u6838\u9178\u9176\u6291\u5236\u5291<\/u>\u00a0<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10086\"><u>\u7e3d\u8f49\u9304\u7d44<\/u><u>cDNA<\/u><u>\u5408\u6210\u8a66\u5291\u76d2<\/u>\u00a0<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10109\">\u75c5\u6bd2RNA\/DNA\u8403\u53d6\u8a66\u5291\u76d2<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=10083\"><u>ultRNA<\/u><u>\u7ba1\u67f1\u7d14\u5316\u8a66\u5291\u76d2<\/u><u>-RNA<\/u><u>\u8403\u53d6<\/u><\/a>\u00a0<\/span><\/p>\n<p><strong>\u7522\u54c1\u61c9\u7528<\/strong><strong>:<\/strong><\/p>\n<ul>\n<li>RNA quantification<br \/>\n\u2022 miRNA detection and analysis<br \/>\n\u2022 Long non-coding RNA detection and analysis<br \/>\n\u2022 Synthesizing cDNA from ssRNA<br \/>\n\u2022 Constructing cDNA library<br \/>\n\u2022 Sequencing dsDNA<br \/>\n\u2022 DNA primer extension<br \/>\n\u2022 Producing template for use in RT-PCR<br \/>\n\u2022 Labelling 3&#8242;-end of duplex DNA via end-filling reactions<br \/>\n\u2022 Generating probes for hybridization<\/li>\n<\/ul>\n<p><span style=\"color: #ff0000;\"><strong>\u76f8\u95dc\u5546\u54c1<\/strong><\/span>\u00a0<span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?news=safeview-%E6%96%B0%E5%9E%8B%E6%A0%B8%E9%85%B8%E5%AE%89%E5%85%A8%E6%9F%93%E5%8A%91\">\u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a> \u00a0| Agarose |\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=2671\">\u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?cat=451\">\u5fae\u751f\u7269\u6297\u751f\u7d20<\/a> \u00a0|\u00a0 <a style=\"color: #3366ff;\" href=\"http:\/\/www.tprobio.com\/?t-pro-plasmid-kit,48\">\u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a> \u00a0|\u00a0 <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?cat=100\">CMOS\u87a2\u5149\u76f8\u6a5f<\/a> \u00a0| <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6196\">Mupid 2 DNA\u6c34\u5e73\u96fb\u6cf3\u69fd<\/a><\/span><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7e3d\u8f49\u9304\u7d44cDNA\u5408\u6210\u8a66\u5291\u76d2 | \u8ca8\u865fG905 100\u500b\u53cd\u61c9 Abmgood\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217RNA\u548cDNA\u7d14\u5316\u8a66\u5291\u76d2 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":10087,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[132],"tags":[3719],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10086"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10086"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10086\/revisions"}],"predecessor-version":[{"id":10115,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10086\/revisions\/10115"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10087"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10086"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10086"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10086"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}