{"id":10019,"date":"2017-05-09T16:04:10","date_gmt":"2017-05-09T08:04:10","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=10019"},"modified":"2022-07-22T10:30:33","modified_gmt":"2022-07-22T02:30:33","slug":"bca%e8%9b%8b%e7%99%bd%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-%e9%82%84%e5%8e%9f%e5%8a%91%e5%85%bc%e5%ae%b9-dtt%ef%bc%8ctcep%e5%92%8c%ce%b2-%e5%b7%b0%e5%9f%ba%e4%b9%99%e9%86%87%e6%a8%a3","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=10019","title":{"rendered":"BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2 \u8a66\u7ba1 (\u9084\u539f\u5291\u517c\u5bb9) | DTT\uff0cTCEP\u548c\u03b2-\u5df0\u57fa\u4e59\u9187\u6a23\u54c1\u7684\u86cb\u767d\u8cea\u6fc3\u5ea6\u5b9a\u91cf \u8ca8\u865fK819-250"},"content":{"rendered":"

\u5546\u54c1: BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u8a66\u7ba1\uff09 \u9084\u539f\u5291\u517c\u5bb9 <\/a><\/span><\/strong>| <\/strong>\u8ca8\u865f<\/strong>K819-250<\/a> <\/span>\u5075\u6e2c\u7bc4\u570d25-2000\u03bcg\/ ml<\/p>\n

Only kit on the market that is compatible with higher conc. of reducing agents TCEP (20 mM), DTT (10 mM), \u03b2-ME (35 mM)<\/em><\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> BioVision\u7684\u9084\u539f\u5291\u517c\u5bb9\u7684BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2\u662f\u76ee\u524d\u5e02\u5834\u4e0a\u552f\u4e00\u53ef\u8207\u5f37\u6548\u9084\u539f\u5291\uff08\u5982\u9ad8\u905420mM\u7684TCEP\uff09\uff0c\u6700\u9ad8\u905410mM\u548c\u9ad8\u905410mM\u7684\u03b2-\u5df0\u57fa\u4e59\u9187\uff08DTT\uff09\u905435mM\u7684\u86cb\u767d\u8cea\u4f30\u8a08\u8a66\u5291\u76d2\u3002\u8a72\u8a66\u5291\u76d2\u4f86\u81eaBioVision\u7684BCA Assay Kit II\uff08\u8ca8\u865f\uff03K813\uff09\uff0c\u57fa\u65bc\u901a\u904e\u9084\u539f\u9285\u967d\u96e2\u5b50\uff08Cu + 2\uff09\u7522\u751f\u7684\u4e8c\u8f9b\u53ef\u5be7\u9178\uff08BCA\uff09\u8207\u4e9e\u9285\u967d\u96e2\u5b50\uff08Cu + 1\uff09\u7684\u87af\u5408\uff09\u8207\u86cb\u767d\u8cea\u5728\u9e7c\u6027\u689d\u4ef6\u4e0b\u3002\u6a23\u54c1\u4e2d\u7684\u9084\u539f\u5291\u5e72\u64feBCA\u6e2c\u5b9a\uff0c\u56e0\u70ba\u5b83\u5011\u80fd\u5920\u6e1b\u5c11Cu + 2\u3002 BioVision\u7684\u9084\u539f\u5291\u517c\u5bb9\u7684BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2\u53ef\u7528\u65bc\u6e2c\u5b9a\u542b\u6709DTT\uff0cTCEP\u548c\u03b2-\u5df0\u57fa\u4e59\u9187\u7684\u6a23\u54c1\u7684\u86cb\u767d\u8cea\u6fc3\u5ea6\u3002\u8a72\u6e2c\u5b9a\u572825-2000\u03bcg\/ ml\u4e4b\u9593\u7684\u86cb\u767d\u8cea\u6fc3\u5ea6\u6700\u5bec\u7bc4\u570d\u5167\u662f\u7dda\u6027\u7684\u3002\u901a\u5e38\uff0c\u53c3\u8003\u5e38\u7528\u7684\u86cb\u767d\u8cea\u6a19\u6e96\u8a08\u7b97\u86cb\u767d\u8cea\u6fc3\u5ea6\u3002\u8a72\u8a66\u5291\u76d2\u9084\u5305\u62ec\u725b\u8840\u6e05\u767d\u86cb\u767d\uff08BSA\uff09\u4f5c\u70ba\u4f30\u8a08\u6a23\u54c1\u7e3d\u86cb\u767d\u8cea\u542b\u91cf\u7684\u86cb\u767d\u8cea\u6a19\u6e96\u3002<\/p>\n

\"\"<\/p>\n

\u86cb\u767d\u8cea\u5b9a\u91cf\u5546\u54c1<\/span> Coomassie blue\u86cb\u767d\u8cea\u5b9a\u91cf\u5206\u6790\u5957\u7d44<\/a> | BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2(\u4f4e\u6fc3\u5ea610-600ug\/ml)| 5\uff05\u8010\u6d17\u6ecc <\/a>| BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2 (\u9084\u539f\u5291\u517c\u5bb9)<\/a><\/span><\/strong><\/p>\n\n\n\n\n\n\n\n\n
Size<\/strong><\/td>\n250 assays<\/td>\n<\/tr>\n
SKU+Size<\/strong><\/td>\nK819-250<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (562 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nMeasuring total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple & convenient.
\n\u2022 Optimized for determining total protein content of samples containing strong reducing agents such as DTT (10 mM), TCEP (20 mM) & \u03b2-mercaptoethanol (35 mM).
\n\u2022 The assay is linear over a wide range of protein concentration (25-2000 \u03bcg\/ml).<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 BCA Reagent A
\n\u2022 BCA Reagent B
\n\u2022 BSA Standard (2 mg\/ml)
\n\u2022 Blocking Reagent (20 mg\/tube)
\n\u2022 Blocking Reagent Buffer<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u5546\u54c1: BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u8a66\u7ba1\uff09 \u9084\u539f\u5291\u517c\u5bb9 | \u8ca8\u865fK819-250 \u5075\u6e2c\u7bc4\u570d25-2000\u03bcg\/ ml […]<\/p>\n","protected":false},"author":3,"featured_media":6512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[89],"tags":[3726],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10019"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10019"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10019\/revisions"}],"predecessor-version":[{"id":10049,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/10019\/revisions\/10049"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6512"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10019"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10019"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10019"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}