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總抗氧化物呈色分析套組 | Total Antioxidant Capacity (TAC) Assay Kit貨號K274-100 (ABCAM ab65329)

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總抗氧化物呈色分析套組操作手冊

特點

Total Antioxidant Capacity (TAC) Colorimetric Assay Kit總抗氧化物呈色分析套組  貨號K274-100(ABCAM ab65329)  | 100個分析

產品描述: Biovision提供一系列抗氧化分析套組;其中總抗氧化物呈色分析套組,以吸收光Absorbance (570 nm)偵;可用於細胞組織lysates、培養液、尿液、血漿、或液體型式的來源。操作時間大約2小時內Applications- Assay measuring either the combination of both small molecule and protein antioxidants or small molecules antioxidant alone in the presence of our proprietary Protein Mask.

Total Antioxidant Capacity (TAC) Colorimetric Assay Kit | BioVision Inc.

產品介紹: 抗氧化劑Antioxidants在防止自由基和其它潛在毒性氧化物質的形成和清除中,扮演重要角色。抗氧化物質三大種類: 酶系統、小分子和蛋白質。酶系統(GSH還原酶(GSH reductase、過氧化氫酶catalase、過氧化物酶peroxidase);小分子(抗壞血酸鹽ascorbate,尿酸uric acid、GSH、維生素E等);和蛋白質(白蛋白albumin、transferrin轉鐵蛋白等)。不同的抗氧化劑的還原能力不同。Trolox用於標準化抗氧化劑,所有其它抗氧化劑在Trolox當量中測量。 測量生物流體和其他樣品的組合的非酶抗氧化能力提供了抵消活性氧(ROS),抵抗氧化損傷和抵抗氧化應激相關疾病的總體能力的指示。在一些情況下,期望蛋白質的抗氧化劑貢獻,而在其他情況下,僅需要小分子抗氧化劑的貢獻。BioVision開發了TAC測定試劑盒,它可以測量小分子抗氧化劑和蛋白質或小分子的組合在我們專有的Protein Mask的存在。 Cu 2+離子通過小分子和蛋白質轉化為Cu +。蛋白質掩蔽物防止蛋白質的Cu2ᶧ還原,使得僅分析小分子抗氧化劑。還原的Cu +離子與比色探針螯合,在570nm附近產生寬的吸收峰,與總抗氧化能力成比例。

總抗氧化物呈色分析套組 | K274-100內容物(ABCAM ab65329)
• Cu²ᶧ Reagent
• Assay Diluent
• Protein Mask
• Trolox Standard

引用文獻

Groner, Judith A. et al. (2016) Oxidative Stress in Youth and Adolescents With Elevated Body Mass Index Exposed to Secondhand Smoke Nicotine Tob Res. 2016 Jul;18(7):1622-7.
Waly et al., Low nourishment of B-vitamins is associated with hyperhomocysteinemia and oxidative stress in newly diagnosed cardiac patients. Experimental Biology and Medicine, Aug 2015; 10.1177/1535370215596860.
Capllonch-Amer et al., Estradiol stimulates mitochondrial biogenesis and adiponectin expression in skeletal muscle.J. Endocrinol., May 2014; 221: 391 – 403.
Karen M. Young et al., Each to their own: skeletal muscles of different function use different biochemical strategies during aestivation at high temperature. J. Exp. Biol., Mar 2013; 216: 1012 – 1024.
Hirai, D. et. al. Acute antioxidant supplementation and skeletal muscle vascular conductance in aged rats: role of exercise and fiber type. Am J Physiol Heart Circ Physiol, Apr 2011; 300: H1536 – H1544.

抗氧化物相關商品

Catalog #  Product Name+  Size
 K671-100  Ascorbic Acid Colorimetric Assay Kit II (FRASC)  100 assays
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 K260-100  GST Fluorometric Activity Assay Kit  100 assays
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 K334-100  HAT Activity Fluorometric Assay Kit  100 assays
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 K712-100  Inosine Fluorometric Assay Kit  100 assays
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 K745-100  Myeloperoxidase (MPO) Fluorometric Activity Assay Kit  100 assays
 K746-200  Myeloperoxidase (MPO) Inhibitor Screening Kit (Fluorometric)  200 assays
 K747-100  Myeloperoxidase (MPO) Peroxidation Activity Assay Kit  100 assays
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 K252-200  Nitric Oxide Fluorometric Assay Kit  200 assays
 K205-100  Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric)  100 assays
 K206-100  Nitric Oxide Synthase (NOS) Activity Assay Kit (Fluorometric)  100 assays
 K208-100  Nitric Oxide Synthase (NOS) Inhibitor Screening Kit (Fluorometric)  100 assays
 K4926-100  NQO1 (human intracellular) ELISA Kit  100 assays
 K772-100  Peroxidase Activity Colorimetric/Fluorometric Assay Kit  100 assays
 K840-100  Protein Disulfide Isomerases (PDI) Inhibitor Screening Kit (Fluorometric)  100 assays
 K335-100  Superoxide Dismutase (SOD) Activity Assay Kit  100 assays
 K763-100  Thioredoxin Reductase Activity Colorimetric Assay Kit  100 assays
 K274-100  Total Antioxidant Capacity (TAC) Colorimetric Assay Kit  100 assays
 K608-100  Uric Acid Colorimetric/Fluorometric Assay Kit  100 assays
 K710-100  Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit  100 assays

 

 

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