5-羥基色氨酸(5-Hydroxytryptophan, 5-HTP) ELISA 試劑盒 | General 5-Hydroxytryptophan(5-HTP) ELISA Kit貨號CK-bio-27605
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General 5-Hydroxytryptophan(5-Hydroxytryptophan, 5-HTP) ELISA Kit (competitive method)
偵測範圍: 10ng/mL -480ng/mL, 最低靈敏度1.0ng/mL
Double speed Elisa 試劑盒在 90 -120分鐘內即可快速獲得實驗結果；競爭法(competitive method)2-3小時。 保持著卓越的偵測專一性與靈敏度。即用型標準品含有6瓶濃度，不需自行配製。每瓶 0.5ml (濃度依不同產品有所不同) 。 Standard (A → F) concentration was followed by: 0ng/mL,30ng/mL ,60ng/mL , 120ng/mL ,240ng/mL ,480ng/mL
產品描述：該試劑盒用於5-羥色氨酸(5-Hydroxytryptophan, 5-HTP) 水平，基於competitive method 。將標準品(Standard)和Sample 加入到預先包被有目標抗體的孔中，然後加入HRP-Conjugate 試劑形成免疫複合物，孵育，通過孵育和洗滌，去除未結合的酶，然後加入底物A和B，溶液會轉藍色，最後在酸的作用下變成黃色。顏色深度或光是與5-羥基色氨酸(5-Hydroxytryptophan, 5-HTP) 濃度呈正相關。偵測範圍: 10ng/mL -480ng/mL , 最低靈敏度1.0ng/mL。可應用於以下樣品來源:血清(Serum)、血漿(plasma)、尿液(urine)、唾液(saliva)、組織勻漿(tissue homogenates)、細胞裂解物(cell lysates)、細胞培養物上清液(cell culture supernates)和其他生物體(biological fluids)液。
5-羥基色氨酸(5-Hydroxytryptophan, 5-HTP) ELISA 試劑盒
For the quantitative determination of5-Hydroxytryptophan concentrations in serum, plasma, saliva, urine, tissue homogenate, cell culture supernates and other biological fluids.
| Name | General 5-Hydroxytryptophan(5-Hydroxytryptophan, 5-HTP) ELISA Kit |
| Cat No. | CK-bio-27605 |
| Specification | 48T/96T/5*96T/10*96T |
| Detection range | 10ng/mL -480ng/mL |
| Detection limit | 1.0ng/mL |
| Test principle | Quantitative |
| Test method | competitive method |
| Storage | The unopened kit should be stored at 2 – 8°C. After opening, please store refer to the manual. |
| Sample type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. |
樣品準備要求 (Specimen Requirements)-血清/血漿/細胞/尿液 (實際操作以原廠操作手冊為主)
5天內使用的樣品可在2-8℃保存，否則樣品必須在-20℃（≤1個月）或-80℃（≤2個月）保存，以免生物活性喪失，避免污染。
1.血清 (Serum)：
讓(Serum)在室溫下凝結10-20分鐘。離心（2000-3000 RPM）20 分鐘。仔細收集上清液(supernatants)。儲存過程中出現沉澱時，應重新離心。
2.血漿(Blood plasma)：
根據樣本採集要求，使用EDTA或檸檬酸鈉(sodium citrate)抗凝(anti coagulation)。添加 EDTA 或檸檬酸鈉並混合 10-20 分鐘。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。儲存過程中出現沉澱時，應重新離心。
3.尿液(Urine)：
用無菌管收集。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。儲存過程中出現沉澱時，應重新離心。採集胸腹水、腦脊液時，請按上述程序進行。
4.細胞培養上清液(Cell culture supernatant)：
檢查分泌成分(secrete components)時用無菌管收集。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。檢查細胞內成分時，用PBS（PH 7.2-7.4）將細胞懸液稀釋至細胞濃度約100萬/ml。通過反覆凍融循環損壞細胞，釋放內部成分。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。儲存過程中出現沉澱時，應重新離心。
5.組織樣品(Tissue sample)：
切取樣品並稱重。添加一定量的PBS（PH 7.4）。立即用液氮冷凍備用。解凍樣品並保存在2-8℃。加入一定量的PBS（PH 7.4），然後用手或均質器將樣品徹底均質。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。等分並保留一份用於檢查，其餘的冷凍以備後用。
Coon-koon台灣代理商-BRAND/AGENCY/代理品牌-Coon-koon
http://www.coonkoonbio.com/
Shanghai Coon Koon Biotech Co.,Ltd是一家專業從事生物製品研發、生產和經營的綜合性生物公司！ 產品遠銷多個國家Coon Koon Bio主要做ELISA試劑盒、生化試劑、抗體等上萬種科研產品！涉及分子生物學、細胞生物學、免疫學等各個領域！堅持“以質量為前提，以客戶滿意為宗旨”；確立“以客戶需求為導向，以市場需求為導向，不斷開發優質新產品，提供客戶滿意。
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