人類熱休克蛋白 27 (HSP27) ELISA 試劑盒 | Human Heat Shock Protein 27 (HSP27) ELISA Kit 貨號:CK-bio-20774
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人類熱休克蛋白 27 (HSP27) ELISA 試劑盒 | Human Heat Shock Protein 27 (HSP27) ELISA Kit 貨號:CK-bio-20774
Human Heat Shock Protein 27 (HSP27) ELISA Kit
偵測範圍: 10pg/ml -600pg/ml , 最低靈敏度1.0pg/ml
Double speed Elisa 試劑盒在 90 -120分鐘內即可快速獲得實驗結果；競爭法(competitive method)2-3小時。 保持著卓越的偵測專一性與靈敏度。即用型標準品含有6瓶濃度，不需自行配製。每瓶 0.5ml (濃度依不同產品有所不同) 。 Standard (A → F) concentration was followed by: 0pg/ml ,37.5pg/ml ,75pg/ml , 150pg/ml ,300pg/ml ,600pg/ml.
產品描述：該試劑盒用於熱休克蛋白 27 (Shock Protein 27, HSP27) 水平，基於雙重原理(sandwich technology)抗體夾心技術酶聯免疫吸附試驗（enzyme linked immunosorbent assay , ELISA）。將標準品(Standard)和Sample 加入到預先包被有目標抗體的孔中，然後加入HRP-Conjugate 試劑形成免疫複合物，孵育，通過孵育和洗滌，去除未結合的酶，然後加入底物A和B，溶液會轉藍色，最後在酸的作用下變成黃色。顏色深度或光是與熱休克蛋白 27 (Shock Protein 27, HSP27) 濃度呈正相關。偵測範圍: 10pg/ml -600pg/ml , 最低靈敏度1.0pg/ml 。可應用於以下樣品來源:血清(Serum)、血漿(plasma)、尿液(urine)、唾液(saliva)、組織勻漿(tissue homogenates)、細胞裂解物(cell lysates)、細胞培養物上清液(cell culture supernates)和其他生物體(biological fluids)液。
人類熱休克蛋白 27 (HSP27) ELISA 試劑盒
For the quantitative determination of Heat Shock Protein 27 (HSP27) concentrations in serum, plasma, saliva, urine, tissue homogenate, cell culture supernates and other biological fluids.
| Name | Human Heat Shock Protein 27 (HSP27) ELISA Kit |
| Cat No. | :CK-bio-20774 |
| Specification | 48T/96T/5*96T/10*96T |
| Detection range | 10pg/ml -600pg/ml |
| Detection limit | 1.0pg/ml |
| Test principle | Quantitative |
| Test method | Sandwich |
| Storage | The unopened kit should be stored at 2 – 8°C. After opening, please store refer to the manual. |
| Sample type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. |
樣品準備要求 (Specimen Requirements)-血清/血漿/細胞/尿液 (實際操作以原廠操作手冊為主)
5天內使用的樣品可在2-8℃保存，否則樣品必須在-20℃（≤1個月）或-80℃（≤2個月）保存，以免生物活性喪失，避免污染。
1.血清 (Serum)：
讓(Serum)在室溫下凝結10-20分鐘。離心（2000-3000 RPM）20 分鐘。仔細收集上清液(supernatants)。儲存過程中出現沉澱時，應重新離心。
2.血漿(Blood plasma)：
根據樣本採集要求，使用EDTA或檸檬酸鈉(sodium citrate)抗凝(anti coagulation)。添加 EDTA 或檸檬酸鈉並混合 10-20 分鐘。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。儲存過程中出現沉澱時，應重新離心。
3.尿液(Urine)：
用無菌管收集。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。儲存過程中出現沉澱時，應重新離心。採集胸腹水、腦脊液時，請按上述程序進行。
4.細胞培養上清液(Cell culture supernatant)：
檢查分泌成分(secrete components)時用無菌管收集。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。檢查細胞內成分時，用PBS（PH 7.2-7.4）將細胞懸液稀釋至細胞濃度約100萬/ml。通過反覆凍融循環損壞細胞，釋放內部成分。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。儲存過程中出現沉澱時，應重新離心。
5.組織樣品(Tissue sample)：
切取樣品並稱重。添加一定量的PBS（PH 7.4）。立即用液氮冷凍備用。解凍樣品並保存在2-8℃。加入一定量的PBS（PH 7.4），然後用手或均質器將樣品徹底均質。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。等分並保留一份用於檢查，其餘的冷凍以備後用。
Coon-koon台灣代理商-BRAND/AGENCY/代理品牌-Coon-koon
http://www.coonkoonbio.com/
Shanghai Coon Koon Biotech Co.,Ltd是一家專業從事生物製品研發、生產和經營的綜合性生物公司！ 產品遠銷多個國家Coon Koon Bio主要做ELISA試劑盒、生化試劑、抗體等上萬種科研產品！涉及分子生物學、細胞生物學、免疫學等各個領域！堅持“以質量為前提，以客戶滿意為宗旨”；確立“以客戶需求為導向，以市場需求為導向，不斷開發優質新產品，提供客戶滿意。
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