植物原花青素含量測定試劑盒 | Plant Proanthocyanidins Content Assay Kit 貨號AK0447-100T-48S

Plant Proanthocyanidins Content Assay Kit

低聚原花青素（Oligomeric proantho cyanidins, OPC）是黃烷醇單體( flavanol monomer )和聚合物(polymer)的多酚化合物(polyphenol compound)，廣泛存在於植物的各個organs中。具有很強的抗氧化性(oxidation resistance)和清除自由基(scavenging free radical)的能力。廣泛應用於醫藥(pharmaceutical)、食品( food)、化妝品(cosmetics, health care products and so on. Under acidic conditions， resorcinol and pyrogallol in A ring of plant OPC can react with vanillin to form colored compound, which can be detected by colorimet)、保健品(health care products)等領域。在酸性條件下，植物OPC A環上的間苯二酚和連苯三酚可與香草醛反應生成有色化合物，可在500 nm比色法檢測並計算OPC的含量。

Plant Proanthocyanidins Assay Kit
Note: Take two or three different samples for prediction before test.
Operation Equipment: Spectrophotometer/microplate Reader
Catalog Number: AK0447
Size:100T/48S

一、樣品製備：

1. 將樣品乾燥至恆重，粉碎，用 40 目篩過濾，加入 1 mL 萃取液至 0.1 g 樣品，超音波（功率 300W，工作時間 5s，間隔 8s）30 min，12000 rpm、25℃離心10 min。將萃取液加至上清液中，定容至1mL供測試。

二.測定步驟

1. 預熱分光光度計/酶標儀 30 分鐘，調節波長至 500 nm，用蒸餾水將計數器調零。

2. 以萃取液將原花青素標準品稀釋至10 mg/mL備用。以萃取液稀釋至最終濃縮物 5、2.5、1.25、0.625、0.3125、0. 156、0.078、0.039、0.02、0.01 mg/mL。

3. 加入以下試劑：

充分混勻，30℃水浴30 min，吸取200 μL至微量比色皿/96孔平底板，在500 nm處檢測吸光度，ΔA（標準）=ΔA（S）=A1-A2，ΔA（測試）= ΔA(T)=A3-A4。空白管只需測試一兩次。
Mix thoroughly, 30℃ water bath for 30 min, take 200 μL to micro cuvette/96 well flat-bottom plate, detect absorbance at 500 nm, ΔA(Standard)=ΔA(S)=A1-A2, ΔA(Test)=ΔA(T) =A3-A4. Blank tube just test once or twice.

Sunlong Biotech 台灣代理商-Sunlong Biotech 

Sunlong Biotech 位於中國杭州，專業從事elisa試劑盒，診斷試劑盒，抗體，抗原，生命科學研究的食品安全卡。 全面的產品線支持所有主要生命科學研究領域的應用，包括分子生物學，免疫學，細胞生物學，蛋白質組學和基因組學研究。Sunlong 擁有自己的品牌，同時與美國USCNK，Cusabio，Eiaab，Bluegene，Bioss等國內知名廠商合作，將所有生物行業知名品牌產品融入中國。Sunlong已經將產品延伸至全球二十多個國家，並與海外企業建立了業務關係，並與合作夥伴建立了互利的關係。 Sunglong Biotech將通過提供廣泛的生命科學研究試劑，試劑盒和技術來提高生產力並為您提供幫助。

AK0447-100T-48S	Plant Proanthocyanidins Content Assay Kit	植物原花青素(OPC)含量檢測試劑盒
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