磷脂測定試劑盒| Phospholipid Assay Kit貨號Abnova™KA1635
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磷脂測定試劑盒| Phospholipid Assay Kit貨號Abnova™KA1635
Colorimetric Assay: 3 to 200 uM, Fluorimetric Assay: 0.6 to 20 uM
100 assays
磷脂檢測試劑盒(The Phospholipid Assay Kit )提供了一種簡單、直接且高通量的檢測方法,用於測量生物樣本中的含膽鹼磷脂(choline-containing phospholipids)。在此測定中,磷脂例如卵磷脂(lecithin)、溶血卵磷脂(lysolecithin )和鞘磷脂(sphingomyelin)被酶水解為膽鹼(choline),使用膽鹼氧化酶(choline oxidase )和 H2O2 特異性染料測定膽鹼( cholin)。粉紅色產物在 570 nm 處的光密度或螢光強度 (530/585 nm) 與樣品中的磷脂濃度成正比。
應用:
✓ 應用:
檢測:生物樣本中的磷脂,例如血清和非 EDTA 血漿。藥物發現/藥理學:藥物對含膽鹼磷脂代謝的影響。
特點:
✓主要特性 靈敏:使用 20 µL 樣品。線性偵測範圍:比色測定 3 - 200 μM,螢光測定 0.6 - 20 μM 磷脂質。
Product Description
Phospholipid Assay Kit is a quantitative colorimetric/fluorimetric phospholipid determination.
Suitable Sample
Plasma (no EDTA), Serum
Sample Volume
20 uL
Detection Method
Colorimetric, Fluorometric
Assay Type
Quantitative
Calibration Range
Colorimetric Assay: 3 to 200 uM, Fluorimetric Assay: 0.6 to 20 uM
Regulation Status
For research use only (RUO)
Quality Control Testing
Standard Curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store the kit at -20°C.