表皮生長因子（EGF），人類重組蛋白 | 貨號4022

Epidermal Growth Factor (EGF), human recombinant

產品介紹: 人表皮生長因子（Human epidermal growth factor , EGF）也稱為HOMG4和URG，是一種通過與受體EGFR結合而在調節細胞生長(cell growth)，增殖(proliferation)和分化(differentiation)中起重要作用的生長因子。表皮生長因子(Epidermal growth factor)存在於人類血小板(human platelets)，巨噬細胞(macrophages)，尿液(urine)，唾液(saliva)，牛奶(milk)和血漿(plasma)中。 EGF是蛋白質EGF家族(EGF-family)的創始成員。該蛋白質家族的成員具有高度相似的結構和功能特徵。所有家族成員均包含一個或多個保守氨基酸序列的重複。唾液EGF的生物學作用包括口腔和胃食管潰瘍的癒合，胃酸分泌的抑制，DNA合成的刺激以及粘膜保護免受腔內傷害因素（如胃酸，膽汁酸，胃蛋白酶和胰蛋白酶）以及物理，化學作用的影響。和細菌劑。由於EGF致癌的風險增加，因此抑制它可以降低癌症的風險。重組人EGF是一個6.2 kDa蛋白，含有53個氨基酸殘基。該重組人EGF在53個氨基酸序列之前具有N端His-tag（MW = 8.5 kDa）

Alternate Name	Urogastrone, URG, EGF, epidermal growth factor
Gene Symbol	EGF
Gene ID	UniGeneHs.419815
Accession #	Q6QBS2
Source	E. coli
Appearance	Recombinant EGF is available as a lyophilized powder
Physical Form Description	EGF protein is lyophilized with no additives
Molecular Weight	Human EGF is a 6.2 kDa recombinant protein
Purity by SDS-PAGE	≥98%
Purity by	≥98%
Endotoxin Level	<0.1 ng/μg
Biological Activity	The ED₅₀ of human EGF is determined by the dose-dependent stimulation of BALB/c 3T3 cells is 5.92-10.06 ng/ml.
Reconstitution Instructions	Reconstitute the human EGF in H₂O to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
Handling	Centrifuge the vial prior to opening.
Storage Conditions	-20°C
Shipping Conditions	Gel Pack
USAGE	For Research Use Only! Not to be used in humans

引用文獻

Wang et al., Evaluation of emulsion electrospun polycaprolactone/hyaluronan/epidermal growth factor nanofibrous scaffolds for wound healing. J Biomater Appl, May 2015; 10.1177/0885328215586907.

An, J. et al. Extensive Determination of Glycan Heterogeneity Reveals an Unusual Abundance of High Mannose Glycans in Enriched Plasma Membranes of Human Embryonic Stem Cells, Mol. Cell. Proteomics, Apr 2012; 11: M111.010660.