3T3-L1分化試劑盒 | 貨號K579-100 (ABCAM ab287843)

3T3-L1 Differentiation Kit

3T3-L1細胞 (3T3-L1 cells ) 源自小鼠3T3細胞，為脂肪生理 (adipose physiology ) 和代謝疾病(metabolic diseases) 的研究提供了一個廣泛使用的基礎模型。它們在分化前表現出成纖維細胞(fibroblast-like) 樣形態，但在分化開始幾天后變得圓潤( rounded ) 並積累脂滴( lipid droplets) 。 累積的脂滴( lipid droplets) 可通過光學顯微鏡觀察。 體外分化的3T3-L1脂肪細胞產生類似於組織來源的脂肪細胞的特徵，並且已經被廣泛用於研究脂肪 形成(adipogenesis)，脂肪分解(lipolysis)和代謝功能障礙(metabolic dysfunctions)。 BioVision的3T3-L1分化試劑盒 (BioVision’s 3T3-L1 Differentiation Kit , K579) 提供了足夠的補充劑，可以製備100毫升的分化培養基 ( differentiation medium ) 和600毫升的維持培養基(maintenance medium ) ，這種培養基對於12個100毫米的培養皿是足夠的材料。 分化雞尾酒在分化培養基中提供1.5μg/ ml胰島素，1μM dexamethasone，500μM IBMX和1μM rosiglitazone的終濃度。

Cat # +Size	K579-100 (ABCAM ab287843)
Size	For 100 ml differentiation medium
Applications	Differentiation of 3T3-L1 preadipocytes to adipocytes Study of obesity, adipogenesis, lipolysis and lipid metabolism
Features & Benefits	• Simple, rapid & convenient method to differentiate 3T3-L1 preadipocytes to adipocytes
Kit Components	• Insulin (1.5 mg/ml) • Differentiation Cocktail 1000x (Lyophilized) • DMSO (anhydrous)

3T3-L1分化試劑盒 | 前脂肪細胞分離試劑盒 | 脂肪組織萃取套組 | 

 

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