【DNA合成/細胞增殖檢測】 EdU Image試劑盒-BrdU分析新型替代方法

細胞增殖(cell proliferation)的檢測，對於評估細胞健康(cell health)，確定遺傳毒性(genotoxicity)或評估抗癌藥物(anticancer drugs)至關重要。到目前為止，直接測量DNA合成(DNA synthesis)是最準確的方法，通常是通過在複製過程中將[3H]胸苷(thymidine)或5-溴-2′-脫氧尿苷(5-bromo-2’-deoxyuridine)等核苷類似物(nucleoside analog like)摻入細胞中，然後通過放射自顯影或分別使用抗BrdU抗體(anti-BrdU-antibody)。EdU（5-乙炔基-2′-脫氧尿苷）是BrdU（5-bromo-2′-脫氧尿苷）的新型替代品。它被取代胸苷摻入到新合成的增殖細胞DNA中。將5-EdU摻入到增殖細胞中，然後使用點擊連接用螢光疊氮化物進行檢測。與BrdU的使用相反，新方法不需要樣品固定或DNA變性。可用於研究中樞神經系統中的細胞增殖。

細胞增殖EdU Image試劑盒 | Cell Proliferation EdU Image Kit貨號KTA2030

細胞增殖EdU Image試劑盒(Cell Proliferation EdU Image Kit, 貨號KTA2030)（綠色螢光）是BrdU分析的一種新型替代方法。 EdU（5-乙炔基-2′-脫氧尿苷）是胸苷的核苷類似物，在活性DNA合成過程中被摻入DNA中。與BrdU分析相比，EdU-Click分析不是基於抗體的，因此不需要DNA變性(denaturation)（通常使用HCl或加熱或用DNase消化）來檢測摻入的核苷。檢測基於click reaction，疊氮化物和炔烴之間的銅催(copper-catalyzed)化共價反應在30分鐘內完成。

Product name	EdU Cell Proliferation Image Kit (Green Fluorescence)
Kit components	• EdU (10mM) • AbFluor 488 azide • 10×Reaction buffer • Copper • Reducing Agent
Features & Benefits	• No antibody needed. • No denaturation steps, preservation of cell morphology and DNA integrity. • Simple, reliable and time-saving than traditional methods. • Proprietary AbFluor 488 azide (Ex/Em = 501/525 nm)-good photostability and minimizing fluorescence quenching. • Optimized for fluorescent microscopy.
Storage instructions	Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.
Shipping	Gel pack with blue ice.
Background	The detection of cell proliferation is of utmost importance for assessing cell health, determining genotoxicity or evaluating anticancer drugs. Until now, measuring DNA synthesis directly is most accurate method of doing it, normally performed by incorporation of the nucleoside analog like [3H] thymidine or 5-bromo-2’-deoxyuridine to cells during replication, and then detected or visualized by autoradiography or with an anti-BrdU-antibody respectively.

In vitro mitochondrial-targeted antioxidant peptide induces apoptosis in cancer cells. W Zhan, X Liao, L Li. Onco Targets Therv.12

Perilaldehyde activates AMP‐activated protein kinase to suppress the growth of gastric cancer via induction of autophagy

Y Zhang, S Liu, Q Feng. JOURNAL OF CELLULAR BIOCHEMISTRY

活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 

貨號	產品名稱	胞器染色位置	螢光波段
貨號KTC4003	TraKine™粒線體染色	粒線體(mitochondria)	Ex / Em = 490/523 nm	增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100	Pro活細胞微管蛋白染色	微管蛋白(Tubulin)	Ex/Em = 500/520 nm	活細胞或固定之細胞
貨號KTC4001	Abbkine 細胞膜染色	細胞膜 (plasma membrane)	Ex/Em = 484/501 nm	活細胞、固定懸浮、貼附細胞
貨號BMD00063	DAPI Staining Solution	細胞核 dsDNA	Ex/Em: 358/461 nm (with DNA)	染色活細胞和固定細胞
貨號KTC4210	活細胞Lysosome染色	Lysosome	deep red fluorescent dye , Ex/Em:650/665nm	染色活細胞和固定細胞