{"id":9966,"date":"2017-05-08T13:03:47","date_gmt":"2017-05-08T05:03:47","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9966"},"modified":"2025-05-16T15:40:12","modified_gmt":"2025-05-16T07:40:12","slug":"%e5%9b%9b%e5%90%88%e4%b8%80%e8%83%9e%e5%99%a8%e8%9b%8b%e7%99%bd%e5%88%86%e9%9b%a2%e8%a9%a6%e5%8a%91%e7%9b%92-fractionprep-cell-fractionation-kit%e8%b2%a8%e8%99%9fk270-50","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9966","title":{"rendered":"\u56db\u5408\u4e00\u80de\u5668\u86cb\u767d\u5206\u96e2\u8a66\u5291\u76d2 | FractionPREP\u2122 Cell Fractionation Kit\u8ca8\u865fK270-50 (ab288085)"},"content":{"rendered":"

\u80de\u5668\u86cb\u767d\u8cea\u5206\u96e2<\/strong>–<\/strong>\u7cfb\u5217\u5546\u54c1\u4ecb\u7d39<\/strong><\/p>\n

\u5546\u54c1<\/u>:<\/u> \u56db\u5408\u4e00\u80de\u5668\u5206\u96e2\u8a66\u5291\u76d2<\/u>FractionPREP\u2122 Cell Fractionation Kit<\/strong>\u8ca8\u865fK270-50 (ABCAM ab288085)<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u8a72\u8a66\u5291\u76d2\u80fd\u5f9e\u4e00\u500b\u54fa\u4e73\u52d5\u7269\u6a23\u672c\u4e2d\u62bd\u63d0\u51fa\u56db\u7a2e\u86cb\u767d\u8cea\uff0c\u7d30\u80de\u8cea\u3001\u7d30\u80de\u6838\u3001\u819c\/\u9846\u7c92\u53ca\u7d30\u80de\u9aa8\u67b6\u7d44\u5206\u7b49\u3002\u65b9\u6cd5\u7c21\u55ae\u3001\u5feb\u901f\uff0c\u6574\u500b\u904e\u7a0b\u53ea\u97002 \u5c0f\u6642\u3001\u4e0d\u9700\u8d85\u9ad8\u901f\u96e2\u5fc3, \u5feb\u901f\u8403\u53d6\u7d30\u80de\u8cea\u3001\u7d30\u80de\u819c\u3001\u7d30\u80de\u6838\u3001\u7d30\u80de\u9aa8\u67b6\u7b49\u7d30\u80de\u5167\u86cb\u767d\u8cea\uff1b\uff1b\u7d50\u679c\u91cd\u8907\u6027\u597d\uff1b \u6240\u5206\u96e2\u7684\u86cb\u767d\u8cea\u53ef\u7528\u65bc\u4e00\u7dad\u6216\u4e8c\u7dad\u96fb\u6cf3\uff0c\u9069\u7528\u65bc\u5404\u985e\u86cb\u767d\u8cea\u5206\u6790\u5be6\u9a57\u3002\u5982SDS-PAGE\u30012D Gel electrophoresis\u3001Western blot\u3001ELISA\u3001EMSA\u3001RNA splicing\u3001translocation\u3001reporter assays\u3001\u9175\u7d20\u6d3b\u6027\u5206\u6790\u7b49\u5be6\u9a57\u3002\u00a0\u61c9\u7528\u7a2e\u985e\uff1a\u7d30\u80de\u3001\u7d44\u7e54\u7686\u9069<\/p>\n

\"cellfractation\"<\/p>\n

\u76f8\u95dc\u5546\u54c1\u00a0<\/span><\/span>\u86cb\u767d\u9176\u548c\u78f7\u9178\u9176\u6291\u5236\u5291 <\/a>| EZBlock\u2122\u78f7\u9178\u9176\u6291\u5236\u5291<\/a> | Ser \/ Thr\u548c\u9e7c\u6027\u78f7\u9178\u9176Inhibitor <\/a>| Protease\u86cb\u767d\u9176\u6291\u5236\u5291<\/a><\/span><\/strong><\/p>\n\n\n\n\n\n\n\n\n
Size<\/strong><\/td>\n50 assays<\/td>\n<\/tr>\n
SKU+Size<\/strong><\/td>\nK270-50<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes less than 1 hour
\n\u2022 Fast and convenient
\n\u2022 The kit is designed for serial sample preparation of four distinct protein fractions including cytosol\/particulate\/cytoskeleton\/nuclear fractions, from one sample.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Cytosol Extraction Buffer (CEB)
\n\u2022 Membrane Extraction Buffer-A (MEB-A)
\n\u2022 Membrane Extraction Buffer-B (MEB-B)
\n\u2022 Nuclear Extraction Buffer (NEB)
\n\u2022 DTT (1 M)
\n\u2022 Protease Inhibitor Cocktail (lyophilized)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u80de\u5668\u86cb\u767d\u5206\u96e2\u7684\u61c9\u7528<\/strong><\/p>\n

    \n
  1. \u63a2\u8a0e\u7d30\u80de\u6838\u5167\u8f49\u9304\u56e0\u5b50\u548c\u7279\u5b9aDNA\u5e8f\u5217\u76f8\u4e92\u4f5c\u7528\u3002\u6aa2\u6e2c\u86cb\u767d\u8cea\u548c\u4e00\u6bb5\u7279\u5b9aDNA\u5e8f\u5217(label)\u76f8\u4e92\u7d50\u5408\u7684\u6280\u8853\u539f\u7406: \u7576\u7279\u5b9a\u5e8f\u5217\u7684DNA\u548c\u67d0\u86cb\u767d\u8cea\u7d50\u5408\u5f8c\uff0c\u6574\u500b\u86cb\u767d\u8cea\u8907\u5408\u9ad4\u5206\u5b50\u91cf\u6703\u589e\u52a0,\u7d93\u7531\u96fb\u6cf3\u5f8c,\u5728gel\u4e2d\u7684\u79fb\u52d5\u901f\u5ea6\u8b8a\u6162,\u6574\u500bband\u9032\u4e0a\u53ef\u52a0\u5165\u7279\u5b9a\u6297\u9ad4\u6aa2\u6e2c\u662f\u5426\u6709\u7279\u5b9a\u86cb\u767d\u8ceaBinding( Supershift)<\/li>\n
  2. \u7d30\u80de\u51cb\u4ea1\u6642,\u7c92\u7dda\u9ad4\u819c\u96fb\u4f4d\u53bb\u6975\u5316\u7684\u904e\u7a0b\u6703\u589e\u52a0\u819c\u7684\u901a\u900f\u6027\u4e26\u4f7f\u819c\u5167\u86cb\u767d,\u5982Cytochrome C, AIF, Smac\/DIABLO\u7b49\u5206\u5b50\u91cb\u653e\u5230\u7d30\u80de\u8cea\u4e2d,\u5f15\u767ccaspase-9\u6d3b\u5316, \u9020\u6210\u4e00\u9023\u4e32\u51cb\u4ea1\u53cd\u61c9\u767c\u751f. COX4\u86cb\u767d\u4f4d\u65bc\u7c92\u817a\u9ad4\u7d30\u80de\u5167\u5074, \u4e26\u5728Apoptosis\u767c\u751f\u6642\u4f9d\u820a\u4fdd\u7559\u5728\u7c92\u7dda\u9ad4\u5167\u3002<\/li>\n
  3. \u4e8c\u7dad\u96fb\u6cf3\u8207MDLC(\u6db2\u76f8\u5c64\u6790)\u4e26\u5217\u70ba\u7814\u7a76\u86cb\u767d\u8cea\u9ad4\u5b78\u7684\u91cd\u8981\u6280\u8853\u4e4b\u4e00\u3002\u539f\u7406: \u5229\u7528\u5206\u5b50\u91cf(\u4e00\u7dad)\u8207\u7b49\u96fb\u9ede(\u4e8c\u7dad)\u4e00\u6b21\u53ef\u5206\u96e2\u5927\u7d042000\u500b\u86cb\u767d\u8cea\u3002\u9650\u5236: \u819c\u86cb\u767d(Hydrophobic)\u5728\u96fb\u6cf3\u4e2d\u4e0d\u6613\u770b\u5230.\u86cb\u767d\u8cea\u82e5\u662f\u70balow abundant protein\u5247\u6b32\u5f97\u5230\u6e96\u78ba\u800c\u53ef\u4fe1\u7684\u5be6\u9a57\u7d50\u679c\u8981\u505a\u80de\u5668\u5206\u96e2( sample prefractionation) \u80de\u5668\u5206\u96e2\u57282d\u61c9\u7528\u7684\u512a\u9ede: \u79fb\u53bbabundant protein\u00a0\u3001\u589e\u52a0 low abundant protein\u3001\u6e1b\u5c11\u86cb\u767d\u8cea\u7684\u8907\u5408\u9ad4\u767c\u751f<\/li>\n<\/ol>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    \u7522\u54c1\u7de8\u865f<\/strong><\/td>\n\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u5305\u88dd<\/strong><\/td>\n<\/tr>\n
    K270-50<\/td>\nFractionPREP\u2122 Cell Fractionation Kit \u56db\u7a2e\u80de\u5668\u8403\u53d6\u4e00\u7d44\u641e\u5b9a<\/td>\n50 assays<\/td>\n<\/tr>\n
    K256-25
    \nK256-100<\/td>\n    		Mitochondria\/Cytosol Fractionation Kit<\/td>\n25 assays
    \n100 assays<\/td>\n<\/tr>\n
    K266-25
    \nK266-100<\/td>\n    		Nuclear\/Cytosol Fractionation Kit<\/td>\n25 assays
    \n100 assays<\/td>\n<\/tr>\n
    K267-50<\/td>\nCytosol\/Particulate Separation Kit<\/td>\n50 assays<\/td>\n<\/tr>\n
    K269-500<\/td>\nMammalian Cell Extraction Kit<\/td>\n500 assays<\/td>\n<\/tr>\n
    K268-50<\/td>\nMembrane Protein Extraction Kit<\/td>\n50 assays<\/td>\n<\/tr>\n
    K288-50<\/td>\nMitochondria Isolation Kit For Tissue & Cultured Cells<\/td>\n50 assays<\/td>\n<\/tr>\n
    K280-50<\/td>\nMitochondrial DNA Isolation Kit<\/td>\n50 assays<\/td>\n<\/tr>\n
    \u5176\u4ed6\u76f8\u95dc\u7522\u54c1<\/strong><\/td>\n<\/tr>\n
    K808-200<\/td>\nDeproteinizing Sample Preparation Kit<\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
    K6570-3<\/td>\nEZExtract\u2122 Polyubiquitin Buffer Kit<\/td>\n\u00a03 components<\/td>\n<\/tr>\n
    K281-50<\/td>\nGenomic DNA Isolation Kit<\/td>\n\u00a050 assays<\/td>\n<\/tr>\n
    K342-25<\/td>\nHDAC1 Immunoprecipitation (IP) & Activity Assay Kit<\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
    \u00a0K341-25<\/td>\nHDAC2 Immunoprecipitation (IP) & Activity Assay Kit<\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
    \u00a0K344-25<\/td>\nHDAC3 Immunoprecipitation (IP) & Activity Assay Kit<\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
    \u00a0K286-25<\/td>\nImmunoprecipitation (IP) Kit<\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
    \u00a0K285-50<\/td>\nMitochondrial Protein IP Kit<\/td>\n\u00a050 assays<\/td>\n<\/tr>\n
    \u00a0K810-1000<\/td>\nProtein Quantitation Kit<\/td>\n\u00a01000 assays<\/td>\n<\/tr>\n
    \u00a0K259-50<\/td>\nYeast Mitochondria Isolation Kit<\/td>\n\u00a050 assays<\/td>\n<\/tr>\n
    \u00a0K289-50<\/td>\nYeast Nuclei Isolation Kit<\/td>\n\u00a050 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u86cb\u767d\u8cea\u5b9a\u91cf\u5546\u54c1<\/strong><\/span> Coomassie blue\u86cb\u767d\u8cea\u5b9a\u91cf\u5206\u6790\u5957\u7d44<\/a> | BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2(\u4f4e\u6fc3\u5ea610-600ug\/ml)| 5\uff05\u8010\u6d17\u6ecc <\/a>| BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2 (\u9084\u539f\u5291\u517c\u5bb9)<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

    \u80de\u5668\u86cb\u767d\u8cea\u5206\u96e2–\u7cfb\u5217\u5546\u54c1\u4ecb\u7d39 \u5546\u54c1: \u56db\u5408\u4e00\u80de\u5668\u5206\u96e2\u8a66\u5291\u76d2FractionPREP\u2122 Cell F […]<\/p>\n","protected":false},"author":3,"featured_media":484,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[179],"tags":[3733],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9966"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9966"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9966\/revisions"}],"predecessor-version":[{"id":51205,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9966\/revisions\/51205"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/484"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9966"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9966"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9966"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}