{"id":9961,"date":"2017-05-08T12:59:42","date_gmt":"2017-05-08T04:59:42","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9961"},"modified":"2022-07-22T10:31:55","modified_gmt":"2022-07-22T02:31:55","slug":"%e7%b4%b0%e8%83%9e%e8%b3%aa%e8%83%9e%e5%85%a7%e9%a1%86%e7%b2%92%e5%bf%ab%e9%80%9f%e5%88%86%e9%9b%a2%e8%a9%a6%e5%8a%91%e7%9b%92-cytosolparticulate-rapid-separation-kit%e8%b2%a8%e8%99%9fk267-50","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9961","title":{"rendered":"\u7d30\u80de\u8cea\/\u80de\u5167\u9846\u7c92\u5feb\u901f\u5206\u96e2\u8a66\u5291\u76d2| Cytosol\/Particulate Rapid Separation Kit\u8ca8\u865fK267-50"},"content":{"rendered":"

\u80de\u5668\u86cb\u767d\u8cea\u5206\u96e2<\/strong>–<\/strong>\u7cfb\u5217\u5546\u54c1\u4ecb\u7d39<\/strong><\/p>\n

\u5546\u54c1<\/u>:<\/u> \u7d30\u80de\u8cea\/<\/strong>\u80de\u5167\u9846\u7c92\u5feb\u901f\u5206\u96e2\u8a66\u5291\u76d2| Cytosol\/Particulate Rapid Separation Kit<\/u>\u8ca8\u865f<\/u>K267-50<\/u><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u86cb\u767d\u8cea\uff0c\u4fe1\u865f\u5206\u5b50\u548c\u7d30\u80de\u5167\u7684\u5176\u4ed6\u5c0f\u5206\u5b50\u7684\u4f4d\u7f6e\u548c\u6613\u4f4d\u8abf\u7bc0\u7d30\u80de\u751f\u9577\uff0c\u5206\u5316\u548c\u8a31\u591a\u5176\u4ed6\u7d30\u80de\u529f\u80fd\u3002 \u5206\u96e2\u7d30\u80de\u8cea\u548c\u9846\u7c92\u7d1a\u5206\u662f\u7814\u7a76\u7d30\u80de\u7d44\u5206\u4e9e\u7d30\u80de\u5b9a\u4f4d\u7684\u91cd\u8981\u4e00\u6b65\u3002 \u7136\u800c\uff0c\u50b3\u7d71\u65b9\u6cd5\u901a\u5e38\u9700\u8981\u6578\u5c0f\u6642\u624d\u80fd\u5f9e\u7d30\u80de\u8cea\u4e2d\u5206\u96e2\u7d30\u80de\u8cea\uff0c\u56e0\u6b64\u5728\u5206\u96e2\u904e\u7a0b\u4e2d\uff0c\u67d0\u4e9b\u7d30\u80de\u6210\u5206\uff0c\u7279\u5225\u662f\u5c0f\u5206\u5b50\u548c\u4ee3\u8b1d\u7269\u6b63\u5728\u64f4\u6563\u6216\u91cd\u65b0\u5206\u4f48\u3002 \u65b0\u7684Cytosol \/ Particulate\u5feb\u901f\u5206\u96e2\u8a66\u5291\u76d2\u5c07\u7d30\u80de\u6eb6\u8cea\u8207\u9846\u7c92\u72c0\u7269\u8cea\u5feb\u901f\u5206\u96e2\uff0c\u901a\u904e\u6cb9\u5c64\uff0c\u56e0\u6b64\u5169\u90e8\u5206\u4e0d\u6703\u76f8\u4e92\u63a5\u89f8\u6216\u64f4\u6563\u3002 \u4f7f\u7528\u8a72\u65b9\u6cd5\uff0c\u5373\u4f7f\u5c0d\u65bc\u5c0f\u5206\u5b50\u4e5f\u53ef\u4ee5\u907f\u514d\u6c61\u67d3\u3002 \u53ef\u4ee5\u6e96\u78ba\u5730\u9032\u884c\u4e9e\u7d30\u80de\u5b9a\u4f4d\u548c\u611f\u8208\u8da3\u56e0\u7d20\u7684\u5206\u6790\u3002<\/p>\n

\"cellfractation\"<\/p>\n

\u76f8\u95dc\u5546\u54c1\u00a0<\/span><\/span>\u86cb\u767d\u9176\u548c\u78f7\u9178\u9176\u6291\u5236\u5291 <\/a>| EZBlock\u2122\u78f7\u9178\u9176\u6291\u5236\u5291<\/a> | Ser \/ Thr\u548c\u9e7c\u6027\u78f7\u9178\u9176Inhibitor <\/a>| Protease\u86cb\u767d\u9176\u6291\u5236\u5291<\/a><\/span><\/strong><\/p>\n\n\n\n\n\n\n\n\n
Size<\/strong><\/td>\n50 assays<\/td>\n<\/tr>\n
SKU+Size<\/strong><\/td>\nK267-50<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe Cytosol\/Particulate Separation Kit provides an easy and convenient system for a rapid separation of cytosol and particulate fractions. Assaying cellular distributions of small moleculars or proteins, and other signal transduction pathways<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes less than 1 hour
\n\u2022 Fast and convenient
\n\u2022 The new Cytosol\/Particulate Rapid Separation Kit physically separate cytosol from particulate compar\u2122ents rapidly through an oil layer and thus the two fractions would not contact or diffuse to each other. Using the method, contaminations can be avoided even for small molecules.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Cell Suspension Buffer
\n\u2022 Cytosol Releasing Buffer
\n\u2022 Oil Layer
\n\u2022 Particulate Layer<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u80de\u5668\u86cb\u767d\u5206\u96e2\u7684\u61c9\u7528<\/strong><\/p>\n

    \n
  1. \u63a2\u8a0e\u7d30\u80de\u6838\u5167\u8f49\u9304\u56e0\u5b50\u548c\u7279\u5b9aDNA\u5e8f\u5217\u76f8\u4e92\u4f5c\u7528\u3002\u6aa2\u6e2c\u86cb\u767d\u8cea\u548c\u4e00\u6bb5\u7279\u5b9aDNA\u5e8f\u5217(label)\u76f8\u4e92\u7d50\u5408\u7684\u6280\u8853\u539f\u7406: \u7576\u7279\u5b9a\u5e8f\u5217\u7684DNA\u548c\u67d0\u86cb\u767d\u8cea\u7d50\u5408\u5f8c\uff0c\u6574\u500b\u86cb\u767d\u8cea\u8907\u5408\u9ad4\u5206\u5b50\u91cf\u6703\u589e\u52a0,\u7d93\u7531\u96fb\u6cf3\u5f8c,\u5728gel\u4e2d\u7684\u79fb\u52d5\u901f\u5ea6\u8b8a\u6162,\u6574\u500bband\u9032\u4e0a\u53ef\u52a0\u5165\u7279\u5b9a\u6297\u9ad4\u6aa2\u6e2c\u662f\u5426\u6709\u7279\u5b9a\u86cb\u767d\u8ceaBinding( Supershift)<\/li>\n
  2. \u7d30\u80de\u51cb\u4ea1\u6642,\u7c92\u7dda\u9ad4\u819c\u96fb\u4f4d\u53bb\u6975\u5316\u7684\u904e\u7a0b\u6703\u589e\u52a0\u819c\u7684\u901a\u900f\u6027\u4e26\u4f7f\u819c\u5167\u86cb\u767d,\u5982Cytochrome C, AIF, Smac\/DIABLO\u7b49\u5206\u5b50\u91cb\u653e\u5230\u7d30\u80de\u8cea\u4e2d,\u5f15\u767ccaspase-9\u6d3b\u5316, \u9020\u6210\u4e00\u9023\u4e32\u51cb\u4ea1\u53cd\u61c9\u767c\u751f. COX4\u86cb\u767d\u4f4d\u65bc\u7c92\u817a\u9ad4\u7d30\u80de\u5167\u5074, \u4e26\u5728Apoptosis\u767c\u751f\u6642\u4f9d\u820a\u4fdd\u7559\u5728\u7c92\u7dda\u9ad4\u5167\u3002<\/li>\n
  3. \u4e8c\u7dad\u96fb\u6cf3\u8207MDLC(\u6db2\u76f8\u5c64\u6790)\u4e26\u5217\u70ba\u7814\u7a76\u86cb\u767d\u8cea\u9ad4\u5b78\u7684\u91cd\u8981\u6280\u8853\u4e4b\u4e00\u3002\u539f\u7406: \u5229\u7528\u5206\u5b50\u91cf(\u4e00\u7dad)\u8207\u7b49\u96fb\u9ede(\u4e8c\u7dad)\u4e00\u6b21\u53ef\u5206\u96e2\u5927\u7d042000\u500b\u86cb\u767d\u8cea\u3002\u9650\u5236: \u819c\u86cb\u767d(Hydrophobic)\u5728\u96fb\u6cf3\u4e2d\u4e0d\u6613\u770b\u5230.\u86cb\u767d\u8cea\u82e5\u662f\u70balow abundant protein\u5247\u6b32\u5f97\u5230\u6e96\u78ba\u800c\u53ef\u4fe1\u7684\u5be6\u9a57\u7d50\u679c\u8981\u505a\u80de\u5668\u5206\u96e2( sample prefractionation) \u80de\u5668\u5206\u96e2\u57282d\u61c9\u7528\u7684\u512a\u9ede: \u79fb\u53bbabundant protein\u00a0\u3001\u589e\u52a0 low abundant protein\u3001\u6e1b\u5c11\u86cb\u767d\u8cea\u7684\u8907\u5408\u9ad4\u767c\u751f<\/li>\n<\/ol>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    \u7522\u54c1\u7de8\u865f<\/strong><\/td>\n\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u5305\u88dd<\/strong><\/td>\n<\/tr>\n
    K270-50<\/td>\nFractionPREP\u2122 Cell Fractionation Kit \u56db\u7a2e\u80de\u5668\u8403\u53d6\u4e00\u7d44\u641e\u5b9a<\/td>\n50 assays<\/td>\n<\/tr>\n
    K256-25
    \nK256-100<\/td>\n    		Mitochondria\/Cytosol Fractionation Kit<\/td>\n25 assays
    \n100 assays<\/td>\n<\/tr>\n
    K266-25
    \nK266-100<\/td>\n    		Nuclear\/Cytosol Fractionation Kit<\/td>\n25 assays
    \n100 assays<\/td>\n<\/tr>\n
    K267-50<\/td>\nCytosol\/Particulate Separation Kit<\/td>\n50 assays<\/td>\n<\/tr>\n
    K269-500<\/td>\nMammalian Cell Extraction Kit<\/td>\n500 assays<\/td>\n<\/tr>\n
    K268-50<\/td>\nMembrane Protein Extraction Kit<\/td>\n50 assays<\/td>\n<\/tr>\n
    K288-50<\/td>\nMitochondria Isolation Kit For Tissue & Cultured Cells<\/td>\n50 assays<\/td>\n<\/tr>\n
    K280-50<\/td>\nMitochondrial DNA Isolation Kit<\/td>\n50 assays<\/td>\n<\/tr>\n
    \u5176\u4ed6\u76f8\u95dc\u7522\u54c1<\/strong><\/td>\n<\/tr>\n
    K808-200<\/td>\nDeproteinizing Sample Preparation Kit<\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
    K6570-3<\/td>\nEZExtract\u2122 Polyubiquitin Buffer Kit<\/td>\n\u00a03 components<\/td>\n<\/tr>\n
    K281-50<\/td>\nGenomic DNA Isolation Kit<\/td>\n\u00a050 assays<\/td>\n<\/tr>\n
    K342-25<\/td>\nHDAC1 Immunoprecipitation (IP) & Activity Assay Kit<\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
    \u00a0K341-25<\/td>\nHDAC2 Immunoprecipitation (IP) & Activity Assay Kit<\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
    \u00a0K344-25<\/td>\nHDAC3 Immunoprecipitation (IP) & Activity Assay Kit<\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
    \u00a0K286-25<\/td>\nImmunoprecipitation (IP) Kit<\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
    \u00a0K285-50<\/td>\nMitochondrial Protein IP Kit<\/td>\n\u00a050 assays<\/td>\n<\/tr>\n
    \u00a0K810-1000<\/td>\nProtein Quantitation Kit<\/td>\n\u00a01000 assays<\/td>\n<\/tr>\n
    \u00a0K259-50<\/td>\nYeast Mitochondria Isolation Kit<\/td>\n\u00a050 assays<\/td>\n<\/tr>\n
    \u00a0K289-50<\/td>\nYeast Nuclei Isolation Kit<\/td>\n\u00a050 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    \u80de\u5668\u86cb\u767d\u8cea\u5206\u96e2–\u7cfb\u5217\u5546\u54c1\u4ecb\u7d39 \u5546\u54c1: \u7d30\u80de\u8cea\/\u80de\u5167\u9846\u7c92\u5feb\u901f\u5206\u96e2\u8a66\u5291\u76d2| Cytosol\/Parti […]<\/p>\n","protected":false},"author":3,"featured_media":484,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[134],"tags":[3735],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9961"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9961"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9961\/revisions"}],"predecessor-version":[{"id":9976,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9961\/revisions\/9976"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/484"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9961"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9961"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9961"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}