{"id":9958,"date":"2017-05-08T12:58:05","date_gmt":"2017-05-08T04:58:05","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9958"},"modified":"2023-05-10T17:00:46","modified_gmt":"2023-05-10T09:00:46","slug":"%e7%b4%b0%e8%83%9e%e6%a0%b8%e7%b4%b0%e8%83%9e%e8%b3%aa%e5%88%86%e9%9b%a2%e8%a9%a6%e5%8a%91%e7%9b%92-nuclearcytosol-fractionation-kit%e8%b2%a8%e8%99%9fk266-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9958","title":{"rendered":"\u7d30\u80de\u6838\/\u7d30\u80de\u8cea\u5206\u96e2\u8a66\u5291\u76d2 | Nuclear\/Cytosol Fractionation Kit\u8ca8\u865fK266-100 (ABCAM, ab289882)"},"content":{"rendered":"

\u80de\u5668\u86cb\u767d\u8cea\u5206\u96e2<\/strong>–<\/strong>\u7cfb\u5217\u5546\u54c1\u4ecb\u7d39<\/strong><\/p>\n

\u7d30\u80de\u6838\/<\/strong>\u7d30\u80de\u8cea\u5206\u96e2\u8a66\u5291\u76d2 Nuclear\/Cytosol Fractionation Kit | <\/u>\u8ca8\u865f<\/u>K266-100(ABCAM, ab289882) <\/u><\/a><\/span><\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"Nuclear\/Cytosol Extraction Kit\u8a66\u5291\u76d2\u5167\u5305\u542b\uff0c\u5206\u96e2\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684\u7d30\u80de\u6838\u548c\u7d30\u80de\u8cea\u6240\u6709\u8a66\u5291\uff0c\u4e0d\u50c5\u80fd\u5920\u5206\u96e2\u7d30\u80de\u6838\u548c\u7d30\u80de\u8cea\u86cb\u767d\u8cea\uff0c\u800c\u4e14\u53ef\u4ee5\u4fdd\u6301\u86cb\u767d\u8cea\u7684\u5b8c\u6574\u6027\u3002\u6240\u5206\u96e2\u7684\u7d30\u80de\u6838\u548c\u7d30\u80de\u8cea\u86cb\u767d\u8cea\u53ef\u9032\u884c\u8f49\u9304\u6d3b\u6027(transcriptional activity)\u3001RNA \u526a\u63a5 (RNA splicing)\u3001\u51dd\u81a0\u963b\u6eef\u96fb\u6cf3\u5be6\u9a57 (gel shift assay)\u3001\u5831\u544a\u86cb\u767d\u6aa2\u6e2c (\u00a0reporter assays)\u3001\u9176\u6d3b\u6027\u6aa2\u6e2c (enzyme activity assays)\u53caWestern Blot \u7814\u7a76\u7b49\u3002<\/p>\n

\"cellfractation\"<\/p>\n

\u76f8\u95dc\u5546\u54c1\u00a0<\/span><\/span>\u86cb\u767d\u9176\u548c\u78f7\u9178\u9176\u6291\u5236\u5291 <\/a>| EZBlock\u2122\u78f7\u9178\u9176\u6291\u5236\u5291<\/a> | Ser \/ Thr\u548c\u9e7c\u6027\u78f7\u9178\u9176Inhibitor <\/a>| Protease\u86cb\u767d\u9176\u6291\u5236\u5291<\/a><\/span><\/strong><\/p>\n

\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\"k266\"<\/a><\/p>\n\n\n\n\n\n\n\n
Detection Method<\/strong><\/td>\nN\/A<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThis Nuclear\/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells and tissues The new kit provides a system that maintains the nuclear and cytoplasmic compartments separate and intact. Transcriptional activity\/reporter assays\/enzyme activity assays\/Western blotting and more downstream assays.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes less than 2-3 hours
\n\u2022 Fast and convenient
\n\u2022 The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic extracts from cells relatively quick with little or no cross-contaminations<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Cytosol Extraction Buffer A (CEB-A)
\n\u2022 Cytosol Extraction Buffer B (CEB-B)
\n\u2022 Nuclear Extraction Buffer (NEB)
\n\u2022 DTT (1 M)
\n\u2022 Protease Inhibitor Cocktail (lyophilized)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u80de\u5668\u86cb\u767d\u5206\u96e2\u7684\u61c9\u7528<\/strong><\/p>\n

    \n
  1. \u63a2\u8a0e\u7d30\u80de\u6838\u5167\u8f49\u9304\u56e0\u5b50\u548c\u7279\u5b9aDNA\u5e8f\u5217\u76f8\u4e92\u4f5c\u7528\u3002\u6aa2\u6e2c\u86cb\u767d\u8cea\u548c\u4e00\u6bb5\u7279\u5b9aDNA\u5e8f\u5217(label)\u76f8\u4e92\u7d50\u5408\u7684\u6280\u8853\u539f\u7406: \u7576\u7279\u5b9a\u5e8f\u5217\u7684DNA\u548c\u67d0\u86cb\u767d\u8cea\u7d50\u5408\u5f8c\uff0c\u6574\u500b\u86cb\u767d\u8cea\u8907\u5408\u9ad4\u5206\u5b50\u91cf\u6703\u589e\u52a0,\u7d93\u7531\u96fb\u6cf3\u5f8c,\u5728gel\u4e2d\u7684\u79fb\u52d5\u901f\u5ea6\u8b8a\u6162,\u6574\u500bband\u9032\u4e0a\u53ef\u52a0\u5165\u7279\u5b9a\u6297\u9ad4\u6aa2\u6e2c\u662f\u5426\u6709\u7279\u5b9a\u86cb\u767d\u8ceaBinding( Supershift)<\/li>\n
  2. \u7d30\u80de\u51cb\u4ea1\u6642,\u7c92\u7dda\u9ad4\u819c\u96fb\u4f4d\u53bb\u6975\u5316\u7684\u904e\u7a0b\u6703\u589e\u52a0\u819c\u7684\u901a\u900f\u6027\u4e26\u4f7f\u819c\u5167\u86cb\u767d,\u5982Cytochrome C, AIF, Smac\/DIABLO\u7b49\u5206\u5b50\u91cb\u653e\u5230\u7d30\u80de\u8cea\u4e2d,\u5f15\u767ccaspase-9\u6d3b\u5316, \u9020\u6210\u4e00\u9023\u4e32\u51cb\u4ea1\u53cd\u61c9\u767c\u751f. COX4\u86cb\u767d\u4f4d\u65bc\u7c92\u817a\u9ad4\u7d30\u80de\u5167\u5074, \u4e26\u5728Apoptosis\u767c\u751f\u6642\u4f9d\u820a\u4fdd\u7559\u5728\u7c92\u7dda\u9ad4\u5167\u3002<\/li>\n
  3. \u4e8c\u7dad\u96fb\u6cf3\u8207MDLC(\u6db2\u76f8\u5c64\u6790)\u4e26\u5217\u70ba\u7814\u7a76\u86cb\u767d\u8cea\u9ad4\u5b78\u7684\u91cd\u8981\u6280\u8853\u4e4b\u4e00\u3002\u539f\u7406: \u5229\u7528\u5206\u5b50\u91cf(\u4e00\u7dad)\u8207\u7b49\u96fb\u9ede(\u4e8c\u7dad)\u4e00\u6b21\u53ef\u5206\u96e2\u5927\u7d042000\u500b\u86cb\u767d\u8cea\u3002\u9650\u5236: \u819c\u86cb\u767d(Hydrophobic)\u5728\u96fb\u6cf3\u4e2d\u4e0d\u6613\u770b\u5230.\u86cb\u767d\u8cea\u82e5\u662f\u70balow abundant protein\u5247\u6b32\u5f97\u5230\u6e96\u78ba\u800c\u53ef\u4fe1\u7684\u5be6\u9a57\u7d50\u679c\u8981\u505a\u80de\u5668\u5206\u96e2( sample prefractionation) \u80de\u5668\u5206\u96e2\u57282d\u61c9\u7528\u7684\u512a\u9ede: \u79fb\u53bbabundant protein\u00a0\u3001\u589e\u52a0 low abundant protein\u3001\u6e1b\u5c11\u86cb\u767d\u8cea\u7684\u8907\u5408\u9ad4\u767c\u751f<\/li>\n<\/ol>\n

    \u5f15\u7528\u6587\u737b<\/p>\n

    \n
    Stephen A. Martin, et al., (2018) GSK3\u03b2 Regulates Brain Energy Metabolism,\u00a0Cell Reports, May 2018, 29768193<\/a><\/div>\n<\/div>\n
    \n
    Liu, Xiang-Qian et al. (2017) Inhibitory effects of 3\u03b1-hydroxy-lup-20(29)-en-23, 28-dioic acid on lipopolysaccharide-induced TNF-\u03b1, IL-1\u03b2, and the high mobility group box 1 release in macrophages,\u00a0Biosci Biotechnol Biochem. 2017 Jul;81(7):1305-1313.<\/a><\/div>\n<\/div>\n
    \n
    Chen, Xiuqing et al. (2017) Attenuation of acute lung injury in a rat model by Semen Cassiae,\u00a0BMC Complement Altern Med. 2017 Apr 28;17(1):234.<\/a><\/div>\n<\/div>\n
    \n
    Cho, Haaglim et al. (2017) ENOblock, a unique small molecule inhibitor of the non-glycolytic functions of enolase, alleviates the symptoms of type 2 diabetes,\u00a0Sci Rep. 2017 Mar 8;7:44186.<\/a><\/div>\n<\/div>\n
    \n
    Oh, Sea-Hun et al. (2017) 4-hydroxybenzaldehyde-chitooligomers suppresses H2O2-induced oxidative damage in microglia BV-2 cells,\u00a0Carbohydr Res. 2017 Feb 22;440-441:32-37.<\/a><\/div>\n
    <\/div>\n<\/div>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    \u7522\u54c1\u7de8\u865f<\/strong><\/td>\n\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u5305\u88dd<\/strong><\/td>\n<\/tr>\n
    K270-50<\/td>\nFractionPREP\u2122 Cell Fractionation Kit \u56db\u7a2e\u80de\u5668\u8403\u53d6\u4e00\u7d44\u641e\u5b9a<\/td>\n50 assays<\/td>\n<\/tr>\n
    K256-25
    \nK256-100<\/td>\n    		Mitochondria\/Cytosol Fractionation Kit<\/td>\n25 assays
    \n100 assays<\/td>\n<\/tr>\n
    K266-25
    \nK266-100<\/td>\n    		Nuclear\/Cytosol Fractionation Kit<\/td>\n25 assays
    \n100 assays<\/td>\n<\/tr>\n
    K267-50<\/td>\nCytosol\/Particulate Separation Kit<\/td>\n50 assays<\/td>\n<\/tr>\n
    K269-500<\/td>\nMammalian Cell Extraction Kit<\/td>\n500 assays<\/td>\n<\/tr>\n
    K268-50<\/td>\nMembrane Protein Extraction Kit<\/td>\n50 assays<\/td>\n<\/tr>\n
    K288-50<\/td>\nMitochondria Isolation Kit For Tissue & Cultured Cells<\/td>\n50 assays<\/td>\n<\/tr>\n
    K280-50<\/td>\nMitochondrial DNA Isolation Kit<\/td>\n50 assays<\/td>\n<\/tr>\n
    \u5176\u4ed6\u76f8\u95dc\u7522\u54c1<\/strong><\/td>\n<\/tr>\n
    K808-200<\/td>\nDeproteinizing Sample Preparation Kit<\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
    K6570-3<\/td>\nEZExtract\u2122 Polyubiquitin Buffer Kit<\/td>\n\u00a03 components<\/td>\n<\/tr>\n
    K281-50<\/td>\nGenomic DNA Isolation Kit<\/td>\n\u00a050 assays<\/td>\n<\/tr>\n
    K342-25<\/td>\nHDAC1 Immunoprecipitation (IP) & Activity Assay Kit<\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
    \u00a0K341-25<\/td>\nHDAC2 Immunoprecipitation (IP) & Activity Assay Kit<\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
    \u00a0K344-25<\/td>\nHDAC3 Immunoprecipitation (IP) & Activity Assay Kit<\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
    \u00a0K286-25<\/td>\nImmunoprecipitation (IP) Kit<\/td>\n\u00a025 assays<\/td>\n<\/tr>\n
    \u00a0K285-50<\/td>\nMitochondrial Protein IP Kit<\/td>\n\u00a050 assays<\/td>\n<\/tr>\n
    \u00a0K810-1000<\/td>\nProtein Quantitation Kit<\/td>\n\u00a01000 assays<\/td>\n<\/tr>\n
    \u00a0K259-50<\/td>\nYeast Mitochondria Isolation Kit<\/td>\n\u00a050 assays<\/td>\n<\/tr>\n
    \u00a0K289-50<\/td>\nYeast Nuclei Isolation Kit<\/td>\n\u00a050 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a>\"ELISA<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

    \u80de\u5668\u86cb\u767d\u8cea\u5206\u96e2–\u7cfb\u5217\u5546\u54c1\u4ecb\u7d39 \u7d30\u80de\u6838\/\u7d30\u80de\u8cea\u5206\u96e2\u8a66\u5291\u76d2 Nuclear\/Cytosol Fract […]<\/p>\n","protected":false},"author":3,"featured_media":484,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[134],"tags":[3736],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9958"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9958"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9958\/revisions"}],"predecessor-version":[{"id":42330,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9958\/revisions\/42330"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/484"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9958"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9958"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9958"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}