{"id":9924,"date":"2017-05-03T16:56:51","date_gmt":"2017-05-03T08:56:51","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9924"},"modified":"2024-01-18T15:39:04","modified_gmt":"2024-01-18T07:39:04","slug":"polyamines%e5%a4%9a%e8%83%ba%e8%9e%a2%e5%85%89%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-total-polyamine-assay-kit-biovision%e8%b2%a8%e8%99%9fk475","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9924","title":{"rendered":"Polyamines\u591a\u80fa\u87a2\u5149\u5206\u6790\u5957\u7d44  | Total Polyamine Assay Kit Biovision\u8ca8\u865fK475( ABCAM ab239728)"},"content":{"rendered":"<p><strong>\u5546\u54c1: <span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=9924\">Polyamines\u591a\u80fa<\/a><\/span><\/strong><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=9924\"><strong>\u87a2\u5149\u5206\u6790\u5957\u7d44<\/strong> <strong><em>\u00a0<\/em><\/strong><strong>| <\/strong><strong>\u8ca8\u865f<\/strong><strong>K475 ( ABCAM ab239728)<\/strong><\/a><\/span><\/p>\n<p>Total Polyamine Assay Kit (Fluorometric)<\/p>\n<p>\u591a\u80fa(Polyamines )\u662f\u5e36\u6709\u5169\u500b\u6216\u591a\u500b\u4f2f\u80fa\u90e8\u5206\u7684\u5c0f\u6709\u6a5f\u5206\u5b50\u3002\u751f\u7269\u591a\u80fa\u5982\u4e9e\u7cbe\u80fa(spermidine)\u3001(putrescine)\u8150\u80fa\u548cand \u7cbe\u80fa(spermine)\uff0c\u4f5c\u70ba\u8de8\u7d30\u80de\u4fe1\u865f\u5206\u5b50\uff0c\u4e26\u53c3\u8207\u591a\u7a2e\u751f\u7269\u904e\u7a0b\u3002\u5118\u7ba1\u5728\u7d30\u80de\u4e2d\u5408\u6210\u4e86\u5c11\u91cf\u7684\u591a\u80fa(Polyamines )\uff0c\u4f46\u7531\u65bc\u8150\u6557\u548c\u8150\u721b\u7684\u7d50\u679c\uff0c\u5e38\u5e38\u6703\u9047\u5230\u66f4\u591a\u7684\u91cf\uff0c\u56e0\u70ba\u5b83\u5011\u662f\u6c28\u57fa\u9178\u5982\u7532\u786b\u6c28\u9178(methionine)\uff0c\u8cf4\u6c28\u9178(lysine)\u548c\u7cbe\u6c28\u9178(arginine)\u812b\u7fa7\u7684\u76f4\u63a5\u7522\u7269\u3002\u9ce5\u6c28\u9178\u812b\u7fa7\u9176(ornithine decarboxylase)\u591a\u80fa\u8150\u80fa( polyamine putrescine)\uff0c\u4e0d\u50c5\u5c0d\u6392\u65a5\u6c23\u5473\u8ca0\u8cac\uff0c\u800c\u4e14\u4e5f\u8207\u764c\u75c7\u6709\u95dc\u3002\u7d30\u80de\u5167\u591a\u80fa(Polyamines )\u6613\u65bc\u7d50\u5408DNA\uff0c\u5c0d\u65bc\u9810\u9632\u6c27\u5316\u6027DNA\u640d\u50b7\u548c\u5f15\u5c0eDNA\u96d9\u93c8\u65b7\u88c2\u4fee\u5fa9\u9014\u5f91\u81f3\u95dc\u91cd\u8981\u3002\u591a\u80fa\u6c34\u5e73\u96a8\u8457\u5e74\u9f61\u7684\u589e\u9577\u800c\u4e0b\u964d\uff0cpolyamine spermidine\u591a\u80fa\u4e9e\u7cbe\u80fa\u7684\u81b3\u98df\u88dc\u5145\u5291\u6700\u8fd1\u5df2\u88ab\u8b49\u660e\u53ef\u4ee5\u6e1b\u5c11\u5e74\u9f61\u76f8\u95dc\u7684\u6c27\u5316\u61c9\u6fc0\u4e26\u5ef6\u9577\u8001\u9f20\u8001\u5316\u6a21\u578b\u7684\u58fd\u547d\u3002BioVision\u7684\u7e3d\u591a\u80fa\u6aa2\u6e2c\u8a66\u5291\u76d2\u53ef\u4ee5\u5feb\u901f\u6e2c\u5b9a\u751f\u7269\u6a23\u54c1\u4e2d\u7684\u591a\u80fa(Polyamines )\u6fc3\u5ea6\u3002\u9078\u64c7\u6027\u9176\u6df7\u5408\u7269\u4f5c\u7528\u65bc\u591a\u80fa\uff0c\u7522\u751f\u904e\u6c27\u5316\u6c2b\uff0c\u7136\u5f8c\u8207\u87a2\u5149\u63a2\u91dd\uff08Ex \/ Em = 535\/587nm\uff09\u53cd\u61c9\u4ee5\u7522\u751f\u8207\u5b58\u5728\u7684\u591a\u80fa\u7684\u91cf\u6210\u6bd4\u4f8b\u7684\u4fe1\u865f\u3002\u8a72\u8a66\u5291\u76d2\u5305\u62ec\u5c08\u6709\u7684\u6a23\u54c1\u6e05\u9664\u8a66\u5291\uff0c\u7528\u65bc\u9810\u8655\u7406\u6a23\u54c1\uff0c\u4ee5\u6d88\u9664\u53ef\u80fd\u5e72\u64fe\u6e2c\u5b9a\u6216\u589e\u52a0\u6a23\u54c1\u80cc\u666f\u7684\u751f\u7269\u6a23\u54c1\u4e2d\u767c\u73fe\u7684\u5e38\u898b\u4ee3\u8b1d\u7269\u3002\u8a72\u6e2c\u5b9a\u6cd5\u662f\u5feb\u901f\uff0c\u7c21\u55ae\u548c\u9ad8\u901a\u91cf\u76f8\u5bb9\u7684\uff0c\u4e26\u4e14\u53ef\u4ee5\u5728\u7d44\u7e54\u88c2\u89e3\u7269\u548c\u5176\u4ed6\u6a23\u54c1\u5982\u553e\u6db2\u4e2d\u6aa2\u6e2c\u4f4e\u81f30.1\u03bcM\u7684\u591a\u80fa(Polyamines )\u6fc3\u5ea6\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.abcam.com\/ps\/products\/239\/ab239728\/Images\/ab239728-319457-total-polyamine-assay-kit-standard-curve.jpg\" alt=\"Polyamine standard curve.\" \/><\/p>\n<p><a href=\" http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2021\/09\/Polyamines\u591a\u80fa\u87a2\u5149\u5206\u6790\u5957\u7d44-\u8ca8\u865fK475.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%8E%9F%E5%BB%A0%E6%93%8D%E4%BD%9C%E4%B8%8B%E8%BC%89-300x75.png\" alt=\"K475\" width=\"143\" height=\"37\" \/><\/a><\/p>\n<table width=\"838\">\n<tbody>\n<tr>\n<td><strong>Size<\/strong><\/td>\n<td>100 assays<\/td>\n<\/tr>\n<tr>\n<td><strong>SKU+Size<\/strong><\/td>\n<td>K475-100 ( ABCAM ab239728)<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Summary<\/strong><\/td>\n<td>\u2022 Detection Method \u2013 Fluorescence (Ex\/Em = 535\/587 nm)<br \/>\n\u2022 Application:<br \/>\n&#8211; Measurement of polyamine content of various tissues\/cell extracts<br \/>\n&#8211; Determination of polyamine concentration in biological fluids<\/td>\n<\/tr>\n<tr>\n<td><strong>Detection Method<\/strong><\/td>\n<td>Fluorescence (Ex\/Em = 535\/587 nm)<\/td>\n<\/tr>\n<tr>\n<td><strong>Species Reactivity<\/strong><\/td>\n<td>Mammalian<\/td>\n<\/tr>\n<tr>\n<td><strong>Applications<\/strong><\/td>\n<td>Measurement of polyamine content of various tissues\/cell extracts<\/td>\n<\/tr>\n<tr>\n<td><strong>Features &amp; Benefits<\/strong><\/td>\n<td>Simple, highly sensitive, high-throughput compatible<\/td>\n<\/tr>\n<tr>\n<td><strong>Kit Components<\/strong><\/td>\n<td>\u2022 Polyamine Assay Buffer<br \/>\n\u2022 Polyamine Probe (in DMSO)<br \/>\n\u2022 Polyamine Enzyme Mix<br \/>\n\u2022Polyamine Developer<br \/>\n\u2022 Sample Clean-Up Mix<br \/>\n\u2022 Polyamine Standard<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/?news=%E3%80%90yihder-lab-20%E5%85%AC%E5%8D%87%E6%81%86%E6%BA%AB%E5%BE%AA%E7%92%B0%E6%B0%B4%E6%A7%BD%E3%80%91%E6%B0%B4%E6%B5%B4%E6%A7%BDwaterbath-%E8%B2%A8%E8%99%9Fbh-230d-yihder\"><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/09\/%E7%8E%BB%E7%89%87%E6%8E%83%E6%8F%8F-300x288.png\" alt=\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\" \/><\/a><a href=\"http:\/\/biopioneer.com.tw\/?cat=564\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%AF%A6%E9%A9%97%E8%A8%AD%E5%82%992-300x288.png\" alt=\"\u5be6\u9a57\u8a2d\u50992\" width=\"300\" height=\"288\" \/><\/a><a href=\"http:\/\/biopioneer.com.tw\/?cat=603\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%AF%A6%E9%A9%97%E8%A8%AD%E5%82%994-300x288.png\" alt=\"\u5be6\u9a57\u8a2d\u50994\" width=\"300\" height=\"288\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5546\u54c1: Polyamines\u591a\u80fa\u87a2\u5149\u5206\u6790\u5957\u7d44 \u00a0| \u8ca8\u865fK475 ( ABCAM ab239728) Tota [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":28510,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261,5380,121,183,3],"tags":[3743],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9924"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9924"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9924\/revisions"}],"predecessor-version":[{"id":9928,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9924\/revisions\/9928"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28510"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9924"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9924"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9924"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}