{"id":9870,"date":"2017-05-02T13:20:02","date_gmt":"2017-05-02T05:20:02","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9870"},"modified":"2022-07-22T10:43:22","modified_gmt":"2022-07-22T02:43:22","slug":"%e5%bd%97%e6%98%9f%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84cometassay-kit-trevigen%e8%b2%a8%e8%99%9f4250-050-k","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9870","title":{"rendered":"2 well \u5f57\u661f\u5206\u6790\u5957\u7d44 | Trevigen CometAssay\u00ae Kit \u8ca8\u865f4250-050-K"},"content":{"rendered":"

Comet Assay\u5f57\u661f<\/strong>\u5206\u6790\u8a66\u5291\u7d44 |\u00a0CometAssay\u00ae Kit<\/strong><\/a><\/span>\uff0825X2 well slides\uff09\u8ca8\u865f#4250-050-K<\/span>
\n<\/strong><\/p>\n

CometAssay\u00ae Kit\u5305\u542b25\u7247\u7279\u5225\u8655\u7406\u7684CometSlides\u2122\uff082\u5b54\uff09\u548c\u8db3\u5920\u7684\u8a66\u5291\uff08Lysis Solution\uff0cLMAgarose\u548c200 mM EDTA pH 8\uff09\uff0c\u7528\u65bc50\u500b\u6e2c\u8a66\u6a23\u54c1\u3002<\/p>\n

DNA\u640d\u50b7\u4ecb\u7d39\uff1b<\/strong>\u6b63\u5e38\u4ee3\u8b1d\u6d3b\u52d5\u548c\u74b0\u5883\u56e0\u7d20\u5f15\u8d77\u7684DNA\u640d\u50b7\uff0c\u5f15\u767cDNA\u640d\u50b7\u53cd\u61c9\u6fc0\u6d3bDNA\u4fee\u5fa9\u9014\u5f91\u3002 \u5728DNA\u4e0d\u53ef\u9006\u4e4b\u60c5\u6cc1\u4e0b\uff0c\u8a98\u5c0e\u7d30\u80de\u7a0b\u5e8f\u51cb\u4ea1\u3002 \u6b63\u5e38DNA\u7d50\u69cb\u5982DNA\u65b7\u88c2\uff0cDNA adducts \u6216\u4ea4\u806f\uff0c\u9700\u8981DNA\u904e\u7a0b\u4f86\u4fee\u5fa9\u3002 DNA\u4fee\u5fa9\u9700\u8981100\u591a\u500b\u57fa\u56e0\u3002Trevigen\u63d0\u4f9b\u4e86DNA\u640d\u50b7\u6aa2\u6e2c\u548cDNA\u4fee\u5fa9\u7814\u7a76\u7684\u5408\u683c\u7522\u54c1\u9078\u64c7\uff0c\u5305\u62ec\u5f57\u661f\u6e2c\u5b9a\u8a66\u5291\u76d2\uff0c\u7528\u65bc\u6e2c\u91cf\u55ae\u7d30\u80deDNA\u640d\u50b7\u3002 \u7528\u65bc\u6e2c\u91cfPARP 1\uff0cPARG\u548cTankyrase 1\u7684\u9ad4\u5916\u9176\u6d3b\u6027\u7684\u8a66\u5291\u76d2\u88ab\u8a2d\u8a08\u7528\u65bc\u7be9\u9078\u5316\u5408\u7269\u6587\u5eab\u4e26\u63d0\u4f9bIC50\u4fe1\u606f\u3002 \u9a57\u8b49\u7684PARP\u548c\u03b3-H2AX ELISA\u6e2c\u5b9a\u53ef\u8207\u8a31\u591a\u6297\u9ad4\uff0cDNA\u4fee\u5fa9\u9176\uff0cDNA\u4fee\u5fa9\u7f3a\u9677\u4e4b\u7d30\u80de\u682a\u548c\u9ad4\u5167\u85e5\u6548\u5b78\u6e2c\u5b9a\u3002<\/p>\n

\u5f57\u661f\u5206\u6790\u7684\u6aa2\u6e2c\u539f\u7406: <\/strong>\u662f\u57fa\u65bc\u8b8a\u6027\u7684\u539f\u7406\uff0c\u5f85\u6e2c\u7d30\u80de\u88ab\u88c2\u89e3\u8655\u7406\u4ee5\u4f7fDNA\u89e3\u65cb\u548c\u8b8a\u6027\uff0c\u4e4b\u5f8c\u7d30\u80de\u6838\u9032\u884c\u96fb\u6cf3\uff0c\u4f7f\u5f97\u7d30\u80de\u5728\u5f57\u661f\u73bb\u7247\u4e0a\u5e73\u92ea\u7684\u4f4e\u7194\u9ede\u74ca\u8102\u7cd6\u4e0a\u79fb\u52d5\uff0c\u4e4b\u5f8c\u4f7f\u7528\u8207DNA\u76f8\u4e92\u4f5c\u7528\u7684\u87a2\u5149\u67d3\u6599\u6216\u9280\u67d3\u8a66\u5291\u9032\u884c \u67d3\u8272\u4f7f\u7d50\u679c\u53ef\u8996\u3002\u5f62\u6210\u7684DNA\u5f57\u661f\u5c3e\u5f62\u72c0\u548c\u9077\u79fb\u6a21\u5f0f\uff0c\u5305\u62ec\u9e7c\u6027\u5f57\u661f\u5c3e\u9577\u5ea6\uff0c\u5c3e\u90e8DNA\u767e\u5206\u6bd4\u4ee5\u53ca\u5c3e\u77e9\u7684\u8a08\u7b97\u7b49\u8cc7\u6599\u53ef\u4f7f\u7528\u5716\u50cf\u5206\u6790\u8edf\u9ad4\u9032\u884c\uff0c\u6700\u7d42\u7528\u65bc\u8a55\u4f30\u7d30\u80de\u4e2dDNA\u7684\u640d\u50b7\u3002Trevigen\u5b8c\u6574\u7684\u5f57\u661f\u5206\u6790\u5be6\u9a57\u65b9\u6848\uff0c\u5305\u62ec\u5f57\u661f\u5206\u6790\u6aa2\u6e2c\u8a66\u5291\u76d2\u3001\u5f57\u661f\u5206\u6790\u967d\u6027\u5c0d\u7167\u7d30\u80de\u3001\u5f57\u661f\u5206\u6790\u8f09\u7247\u3001\u5f57\u661f\u5206\u6790\u6240\u9700\u7684\u5206\u6790\u548c\u67d3\u8272\u8a66\u5291\u4ee5\u53ca\u5f57\u661f\u5206\u6790\u96fb\u6cf3\u7cfb\u7d71\uff0c\u4ee5\u53ca\u5c08\u5229\u7684FLARE\u2122\u6aa2\u6e2c\u8a66\u5291\u76d2\u3002\u53ef\u6eff\u8db3\u60a8\u5f57\u661f\u5206\u6790\u7684\u6240\u6709\u9700\u8981\u3002<\/p>\n

Standard Comet Assay (CometAssay\u00ae) Archives – Trevigen<\/p>\n

\"CometAssay\u00ae<\/a><\/p>\n

\u5f57\u661f\u5206\u6790<\/strong>\u4e4b\u61c9\u7528<\/strong> \u5f57\u661f\u5206\u6790\u53c8\u7a31\u55ae\u7d30\u80de\u51dd\u81a0\u96fb\u6cf3\u5206\u6790\uff0c\u70ba\u6aa2\u6e2c\u7d30\u80de\u5167DNA\u640d\u50b7\u63d0\u4f9b\u7c21\u4fbf\u6709\u6548\u7684\u65b9\u6cd5\u3002\u81ea\u6210\u529f\u5730\u7528\u65bc\u6aa2\u6e2cDNA\u55ae\u93c8\u65b7\u88c2\u4ee5\u4f86\uff0c\u7d93\u4e0d\u65b7\u5730\u6539\u9032\uff0c\u5df2\u6210\u70ba\u4e00\u7a2e\u5feb \u901f\u3001\u9748\u654f\u548c\u7c21\u4fbf\u5730\u6aa2\u6e2cDNA\u640d\u50b7\u5730\u65b9\u6cd5\uff0c\u5ee3\u6cdb\u5730\u61c9\u7528\u65bcDNA\u653e\u5c04\u640d\u50b7\u3001DNA\u526a\u5207\u640d\u50b7\u3001DNA\u4ea4\u806f\u7684\u6aa2\u6e2c\u3001\u85e5\u7269\u7684\u907a\u50b3\u6bd2\u6027\u8a55\u50f9\u548c\u7d30\u80de\u51cb\u4ea1\u9452\u5b9a\u7b49\u5de5\u4f5c\u4e2d\u3002\u666e\u901a\u7684\u5f57\u661f\u5206\u6790\u53ca\u87a2\u5149\u67d3\u8272\u7522\u751f\u7684\u8cc7\u6599\u7d50\u679c\u9700\u8981\u87a2\u5149\u986f\u5fae\u93e1\u9032\u884c\u89c0\u5bdf\uff0c\u4e26\u4e14\u4e0d\u6613\u4fdd\u5b58\uff0c\u5982\u679c\u80fd\u7372\u5f97\u6c38\u4e45\u7684\u5f57\u661f\u5206\u6790\u5716\u50cf\u7d50\u679c\uff0c\u4e26\u53ef\u5728\u666e\u901a\u986f\u5fae\u93e1\u4e0b\u6aa2\u6e2c\u5247\u53ef\u4ee5\u63d0\u9ad8\u5f57\u661f\u5206\u6790\u7684\u4fbf\u6377\u6027\u3002<\/p>\n

    \n
  1. \u9069\u7528\u65bc\u55ae\u934a\u6216\u96d9\u93c8DNA\u65b7\u88c2\u7684\u5206\u6790<\/li>\n
  2. \u7814\u7a76\u7d30\u80de\u5c0dDNA\u640d\u50b7\u7684\u53cd\u61c9<\/li>\n
  3. \u7be9\u9078DNA\u4fee\u5fa9\u6291\u88fd\u5291<\/li>\n
  4. \u6e2c\u8a66\u5316\u5b78\u54c1\u7684\u6bd2\u6027<\/li>\n
  5. \u74b0\u5883\u8a98\u8b8a\u5291\u7be9\u9078<\/li>\n<\/ol>\n

    \u5f57\u661f\u5206\u6790\u64cd\u4f5c\u6d41\u7a0b\uff1a<\/strong><\/p>\n

    \"comet_procedure\"<\/p>\n

      \n
        \n
      1. \u5f85\u6e2c\u7d30\u80de\u8207\u4f4e\u7194\u9ede\u74ca\u8102\u81a0 \uff08LM Agarose\uff09\uff0c\u572837o<\/sup>C\u4e0b\u5747\u52fb\u6df7\u5408\u3002<\/li>\n
      2. \u5c07\u7d30\u80de\u653e\u7f6e\u5728CometSlide\u2122\u4e0a\u3002<\/li>\n
      3. \u7d66\u4e88\u7d30\u80deLysis Solution\uff0c\u79fb\u9664\u7d30\u80de\u819c\u8207DNA\u4e0a\u7684histones\u3002<\/li>\n
      4. \u52a0\u5165\u8a66\u5291\u4f7fDNA\u8b8a\u6027\u3001\u89e3\u65cb\u3002<\/li>\n
      5. \u6a23\u54c1\u4f7f\u7528\u6838\u9178\u67d3\u5291\u9032\u884c\u67d3\u8272\uff0c\u4e26\u4f7f\u7528\u87a2\u5149\u986f\u5fae\u93e1\uff0c\u89c0\u6e2c\u96fb\u6cf3\u5f8c\u65b7\u88c2DNA\u79fb\u52d5\u7684\u60c5\u5f62\u3002\u00a0<\/span><\/li>\n<\/ol>\n<\/ol>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
        \u5546\u54c1\u578b\u865f<\/strong><\/td>\n\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u5305\u88dd\u898f\u683c<\/strong><\/td>\n<\/tr>\n
        Comet Assay<\/strong>\u5206\u6790\u8a66\u5291\u7d44<\/strong><\/td>\n<\/tr>\n
        4250-050-K<\/td>\nCometAssay\u00ae Kit\uff0825X2 well slides\uff09<\/td>\n50 samples<\/td>\n<\/tr>\n
        4252-040-K<\/td>\nCometAssay\u00ae Kit\uff082X20 well slides\uff09<\/td>\n40 samples<\/td>\n<\/tr>\n
        4253-096-K<\/td>\nCometAssay\u00ae Kit\uff081X96 well slide\uff09<\/td>\n96 samples<\/td>\n<\/tr>\n
        Comet Assay<\/strong>\u5c08\u7528\u73bb\u7247<\/strong><\/td>\n<\/tr>\n
        4250-050-03
        \n4250-200-03<\/td>\n        		CometSlides\u2122 \u5c08\u7528\u73bb\u7247\uff082 Well\uff09<\/td>\n25 \u7247
        \n100 \u7247<\/td>\n<\/tr>\n
        4252-040-01
        \n4252-200-01
        \n4252-500-01
        \n4252-02K-01<\/td>\n        		CometAssay\u00ae HT \u5c08\u7528\u73bb\u7247\uff0820 Well\uff09<\/td>\n2 \u7247
        \n10 \u7247
        \n25 \u7247
        \n100 \u7247<\/td>\n<\/tr>\n
        4253-096-03<\/td>\nCometSlide\u2122 \u5c08\u7528\u73bb\u7247\uff0896 Well\uff09<\/td>\n1 \u7247<\/td>\n<\/tr>\n
        Comet Assay <\/strong>\u5c08\u7528\u8a66\u5291<\/strong><\/td>\n<\/tr>\n
        4250-010-01<\/td>\nCometAssay Lysis Solution<\/td>\n100 ml<\/td>\n<\/tr>\n
        4250-050-02<\/td>\nCometAssay\u00ae LM Agarose<\/td>\n15 ml<\/td>\n<\/tr>\n
        Comet Assay <\/strong>\u96fb\u6cf3\u8a2d\u5099<\/strong><\/td>\n<\/tr>\n
        4250-050-ES<\/td>\nCometAssay\u00ae Electrophoresis System II<\/td>\n1\u7d44<\/td>\n<\/tr>\n
        4250-050-ESK<\/td>\nCometAssay\u00ae 2 Well ES Unit w\/ Starter Kit<\/td>\n1\u7d44\u542b\u5957\u7d44<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

        \u66f4\u591a\u76f8\u95dc\u5546\u54c1<\/span> \u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a> | Agarose | \u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a> | \u5fae\u751f\u7269\u6297\u751f\u7d20<\/a> | \u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a> | CMOS\u87a2\u5149\u76f8\u6a5f<\/a> |Mupid 2 DNA\u6c34\u5e73\u96fb\u6cf3\u69fd<\/a>|<\/span><\/p>\n

        \"starter<\/a>\u00a0\u00a0\"CometAssay\u00ae<\/a>\u00a0\u00a0\"CometSlides\u2122\"DNA\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

        Comet Assay\u5f57\u661f\u5206\u6790\u8a66\u5291\u7d44 |\u00a0CometAssay\u00ae Kit\uff0825X2 well slides\uff09\u8ca8 […]<\/p>\n","protected":false},"author":3,"featured_media":10451,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[515,183],"tags":[3749],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9870"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9870"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9870\/revisions"}],"predecessor-version":[{"id":14540,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9870\/revisions\/14540"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/10451"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9870"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9870"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9870"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}