{"id":9841,"date":"2017-04-30T13:32:11","date_gmt":"2017-04-30T05:32:11","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9841"},"modified":"2022-07-22T10:44:10","modified_gmt":"2022-07-22T02:44:10","slug":"8-%e6%b0%a7%e4%bb%a3-%e9%b3%a5%e5%98%8c%e5%91%a4dna%e7%b3%96%e5%9f%ba%e5%8c%96%e9%85%b6%ef%bc%88hogg1%ef%bd%9c%e8%b2%a8%e8%99%9f4130-100-eb","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9841","title":{"rendered":"8-\u6c27\u4ee3 – \u9ce5\u560c\u5464DNA\u7cd6\u57fa\u5316\u9176\uff08hOGG1)\uff5c\u8ca8\u865f4130-100-EB"},"content":{"rendered":"

\u5546\u54c1:<\/strong> 8-\u6c27\u4ee3 – \u9ce5\u560c\u5464DNA\u7cd6\u57fa\u5316\u9176\uff08hOGG1)\uff5c\u8ca8\u865f4130-100-EB<\/p>\n

Human 8-oxoGuanine DNA Glycosylase<\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong> \u4eba8-\u6c27\u4ee3 – \u9ce5\u560c\u5464DNA\u7cd6\u57fa\u5316\u9176\uff08hOGG1\uff09\u50ac\u5316\u9664\u53bb\u52a0\u5408\u72697,8-\u4e8c\u6c2b-8-\u6c27\u57fa\u9ce5\u560c\u5464\uff088-\u6c27\u4ee3G\uff09\u548c2,6-\u4e8c\u6c28\u57fa-4-\u7fa5\u57fa-5-\u7532\u9170\u80fa\u57fa\u5627\u5576\uff08FaPy\uff09\uff0c\u901a\u904e \u5e2d\u592b\u9e7c\u5316\u5b78\u5f8c\u88c2\u89e3DNA\u78f7\u9178\u4e8c\u916f\u9375\u3002 hOGG1\u4e0d\u50cf\u9810\u671f\u90a3\u6a23\u8b58\u52258-\u6c27\u4ee3G\u7684C = O\uff0c\u800c\u662f\u8b58\u5225\u6838\u82f7\u9178N7\u4e0a\u7684\u8cea\u5b50\u3002 \u7531\u65bc\u5fa9\u88fd\u904e\u7a0b\u4e2d\u817a\u560c\u5464\u7684\u932f\u914d\uff0c8-\u6c27\u4ee3G\u6703\u5c0e\u81f4G\uff1aC\u5230T\uff1aA\u7684\u8f49\u63db\uff0c\u9019\u662f\u4eba\u985e\u764c\u75c7\u4e2d\u5e38\u898b\u7684\u9ad4\u7d30\u80de\u7a81\u8b8a\u3002 \u76f8\u6bd4\u4e4b\u4e0b\uff0cFaPy\u75c5\u8b8a\u5c0e\u81f4\u8907\u88fd\u7684\u7d42\u6b62\uff0c\u56e0\u6b64\u4e0d\u88ab\u8a8d\u70ba\u662f\u8a98\u8b8a\u524d\u640d\u50b7\u3002<\/p>\n

DNA Repair<\/strong>\u9175\u7d20\u4ecb\u7d39<\/strong>: <\/strong>DNA\u9e7c\u57fa\u5207\u9664\u4fee\u5fa9\uff08BER\uff09\u7531\u7a31\u70baDNA\u7cd6\u57fa\u5316\u9176\u7684DNA\u4fee\u5fa9\u9176\u5f15\u767c\uff0c\u5176\u5207\u5272\u53d7\u640d\u7279\u5b9a\u6838\u82f7\u9178\u7684\u9e7c\u57fa\u548c\u812b\u6c27\u6838\u7cd6\u4e4b\u9593\u7684\u7cd6\u57fa\u9375\u3002 \u7136\u5f8c\u901a\u904eAP\u5167\u5207\u6838\u9178\u9176\u6216\u7cd6\u57fa\u5316\u9176\u76f8\u95dc\u7684AP\u88c2\u89e3\u9176\u5207\u5272\u9130\u8fd1\u7121\u560c\u5464\/\u812b\u5627\u5576\uff08AP\uff09\u4f4d\u9ede\u7684\u78f7\u9178\u916f\u9aa8\u67b6\u3002 \u9019\u4e9b\u9176\u53ef\u4ee5\u5728\u9ad4\u5916\u7528\u65bc\u6aa2\u6e2c\u7279\u5b9a\u5f62\u5f0f\u7684DNA\u640d\u50b7\u3002 Trevigen\u7368\u7279\u7684FLARE\u2122\u6e2c\u5b9a\u6cd5\u4f7f\u7528\u5404\u7a2eDNA\u4fee\u5fa9\u9176\u8207\u5f57\u661f\u6e2c\u5b9a\u7d50\u5408\u8b58\u5225\u6c27\u5316\u640d\u50b7\uff0c\u8868\u5fb5\u55ae\u7d30\u80de\u4e2d\u7684DNA\u640d\u50b7\u3002\u5404\u7a2eDNA\u4fee\u5fa9\u9176\uff08Fpg\uff0chOGG1\uff0cMutY\uff0cTDG\u548cEndo III\uff09\u53ef\u4f5c\u70ba\u5177\u6709\u512a\u5316\u6e2c\u5b9a\u689d\u4ef6\u548c\u53cd\u61c9\u7de9\u885d\u6db2\u7684\u55ae\u7368\u7d44\u5206\u63d0\u4f9b\uff0c\u7528\u65bc\u5728\u57fa\u5e95\u5207\u9664\u4fee\u5fa9\u548c\u6c27\u5316\u640d\u50b7\u7684\u5340\u57df\u9032\u884c\u7814\u7a76\u3002 Trevigen\u9084\u63d0\u4f9b\u80fd\u5920\u9032\u884ctranslesion\u5408\u6210\u7684\u55dc\u71b1DNA\u805a\u5408\u9176Dpo4\uff0c\u7528\u65bc\u5076\u806fPCR\u81f3\u64f4\u589e\u53d7\u640dDNA\u3002<\/p>\n\n\n\n\n\n\n\n\n\n
DNA Repair Enzymes<\/strong><\/td>\nEnzyme Reaction Buffers<\/strong><\/td>\n<\/tr>\n
Dpo4 Polymerase<\/td>\n10X REC\u2122 Buffer 15\/100 mM MgCl2<\/sub>\/50 mM MnCl2<\/sub><\/td>\n<\/tr>\n
E. coli <\/em>Endonuclease III<\/td>\n10X REC\u2122 Buffer 4<\/td>\n<\/tr>\n
E. coli <\/em>Fpg<\/td>\n10X REC\u2122 Buffer 10<\/td>\n<\/tr>\n
E. coli <\/em>MutY<\/td>\n10X REC\u2122 Buffer 4<\/td>\n<\/tr>\n
hOGG1<\/td>\n10X REC\u2122 Buffer 6<\/td>\n<\/tr>\n
Thermostable TDG<\/td>\n10X REC\u2122 Buffer 4<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1 <\/b><\/span>\u8010\u71b1TDG<\/b><\/span>\u86cb\u767d<\/b><\/span><\/a> <\/b><\/span>\uff5c <\/b>8-<\/b><\/span>\u6c27\u4ee3 – <\/b><\/span>\u9ce5\u560c\u5464DNA<\/b><\/span>\u7cd6\u57fa\u5316<\/b><\/span>\u9176\uff08hOGG1)<\/b><\/span><\/a> <\/b><\/span>\uff5c <\/b>Endonuclease III<\/b><\/span><\/a> |<\/b> DNA<\/b><\/span>\u7cd6\u57fa\u5316\u9176\uff08Fpg<\/b><\/span>\uff09<\/b><\/span><\/a> <\/b><\/span><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u5546\u54c1: 8-\u6c27\u4ee3 – \u9ce5\u560c\u5464DNA\u7cd6\u57fa\u5316\u9176\uff08hOGG1)\uff5c\u8ca8\u865f4130-100-EB Human […]<\/p>\n","protected":false},"author":3,"featured_media":9835,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[121,519],"tags":[3755],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9841"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9841"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9841\/revisions"}],"predecessor-version":[{"id":9849,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9841\/revisions\/9849"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/9835"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9841"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9841"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9841"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}