{"id":9837,"date":"2017-04-30T13:22:08","date_gmt":"2017-04-30T05:22:08","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9837"},"modified":"2022-07-22T11:24:23","modified_gmt":"2022-07-22T03:24:23","slug":"%e6%a0%b8%e9%85%b8%e5%85%a7%e5%88%87%e9%85%b6iii-coli-e-coli-endonuclease-iii-%e8%b2%a8%e8%99%9f4045-01k-eb","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9837","title":{"rendered":"\u6838\u9178\u5167\u5207\u9176III | coli E. coli Endonuclease III \u8ca8\u865f4045-01K-EB"},"content":{"rendered":"

\u5546\u54c1:<\/strong> E. coli Endonuclease III and 10X REC\u2122 Buffer 4\uff5c\u8ca8\u865f4045-01K-EB<\/p>\n

Ecoli<\/em> cEndonuclease III)<\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong> \u5927\u8178\u687f\u83cc\u80f8\u817a\u5627\u5576\u4e8c\u7cd6-DNA\u7cd6\u57fa\u5316\u9176\uff08\u6838\u9178\u5167\u5207\u9176III\uff09\u662f\u5177\u6709\u76f8\u95dcAP\u88c2\u89e3\u9176\u6d3b\u6027\u7684DNA\u7cd6\u57fa\u5316\u9176\u3002 \u5b83\u91cb\u653e\u53d7\u7d2b\u5916\u7dda\uff0c\u96fb\u96e2\u8f3b\u5c04\uff0c\u56db\u6c27\u5316\u92e8\u6216\u9178\u640d\u50b7\u7684\u9e7c\u57fa\uff0c\u4e26\u901a\u904e\u03b2-\u6d88\u9664\u5207\u5272\u6240\u5f97\u7684\u7121\u9e7c\u57fa\u4f4d\u9ede\uff0c\u5f9e\u800c\u5728DNA\u4e2d\u7522\u751f\u55ae\u500b\u6838\u82f7\u9178\u9593\u9699\u3002 \u8a72\u9176\u7684\u5206\u5b50\u91cf\u70ba23.4kDa\u3002<\/p>\n

DNA Repair<\/strong>\u9175\u7d20\u4ecb\u7d39<\/strong>: <\/strong>DNA\u9e7c\u57fa\u5207\u9664\u4fee\u5fa9\uff08BER\uff09\u7531\u7a31\u70baDNA\u7cd6\u57fa\u5316\u9176\u7684DNA\u4fee\u5fa9\u9176\u5f15\u767c\uff0c\u5176\u5207\u5272\u53d7\u640d\u7279\u5b9a\u6838\u82f7\u9178\u7684\u9e7c\u57fa\u548c\u812b\u6c27\u6838\u7cd6\u4e4b\u9593\u7684\u7cd6\u57fa\u9375\u3002 \u7136\u5f8c\u901a\u904eAP\u5167\u5207\u6838\u9178\u9176\u6216\u7cd6\u57fa\u5316\u9176\u76f8\u95dc\u7684AP\u88c2\u89e3\u9176\u5207\u5272\u9130\u8fd1\u7121\u560c\u5464\/\u812b\u5627\u5576\uff08AP\uff09\u4f4d\u9ede\u7684\u78f7\u9178\u916f\u9aa8\u67b6\u3002 \u9019\u4e9b\u9176\u53ef\u4ee5\u5728\u9ad4\u5916\u7528\u65bc\u6aa2\u6e2c\u7279\u5b9a\u5f62\u5f0f\u7684DNA\u640d\u50b7\u3002 Trevigen\u7368\u7279\u7684FLARE\u2122\u6e2c\u5b9a\u6cd5\u4f7f\u7528\u5404\u7a2eDNA\u4fee\u5fa9\u9176\u8207\u5f57\u661f\u6e2c\u5b9a\u7d50\u5408\u8b58\u5225\u6c27\u5316\u640d\u50b7\uff0c\u8868\u5fb5\u55ae\u7d30\u80de\u4e2d\u7684DNA\u640d\u50b7\u3002\u5404\u7a2eDNA\u4fee\u5fa9\u9176\uff08Fpg\uff0chOGG1\uff0cMutY\uff0cTDG\u548cEndo III\uff09\u53ef\u4f5c\u70ba\u5177\u6709\u512a\u5316\u6e2c\u5b9a\u689d\u4ef6\u548c\u53cd\u61c9\u7de9\u885d\u6db2\u7684\u55ae\u7368\u7d44\u5206\u63d0\u4f9b\uff0c\u7528\u65bc\u5728\u57fa\u5e95\u5207\u9664\u4fee\u5fa9\u548c\u6c27\u5316\u640d\u50b7\u7684\u5340\u57df\u9032\u884c\u7814\u7a76\u3002 Trevigen\u9084\u63d0\u4f9b\u80fd\u5920\u9032\u884ctranslesion\u5408\u6210\u7684\u55dc\u71b1DNA\u805a\u5408\u9176Dpo4\uff0c\u7528\u65bc\u5076\u806fPCR\u81f3\u64f4\u589e\u53d7\u640dDNA\u3002<\/p>\n\n\n\n\n\n\n\n\n\n
DNA Repair Enzymes<\/strong><\/td>\nEnzyme Reaction Buffers<\/strong><\/td>\n<\/tr>\n
Dpo4 Polymerase<\/td>\n10X REC\u2122 Buffer 15\/100 mM MgCl2<\/sub>\/50 mM MnCl2<\/sub><\/td>\n<\/tr>\n
E. coli <\/em>Endonuclease III<\/td>\n10X REC\u2122 Buffer 4<\/td>\n<\/tr>\n
E. coli <\/em>Fpg<\/td>\n10X REC\u2122 Buffer 10<\/td>\n<\/tr>\n
E. coli <\/em>MutY<\/td>\n10X REC\u2122 Buffer 4<\/td>\n<\/tr>\n
hOGG1<\/td>\n10X REC\u2122 Buffer 6<\/td>\n<\/tr>\n
Thermostable TDG<\/td>\n10X REC\u2122 Buffer 4<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1 <\/b><\/span>\u8010\u71b1TDG<\/b><\/span>\u86cb\u767d<\/b><\/span><\/a> <\/b><\/span>\uff5c <\/b>8-<\/b><\/span>\u6c27\u4ee3 – <\/b><\/span>\u9ce5\u560c\u5464DNA<\/b><\/span>\u7cd6\u57fa\u5316<\/b><\/span>\u9176\uff08hOGG1)<\/b><\/span><\/a> <\/b><\/span>\uff5c <\/b>Endonuclease III<\/b><\/span><\/a> |<\/b> DNA<\/b><\/span>\u7cd6\u57fa\u5316\u9176\uff08Fpg<\/b><\/span>\uff09<\/b><\/span><\/a> <\/b><\/span><\/span><\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u5546\u54c1: E. coli Endonuclease III and 10X REC\u2122 Buffer 4\uff5c\u8ca8\u865f40 […]<\/p>\n","protected":false},"author":3,"featured_media":9835,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[121,519],"tags":[3757],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9837"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9837"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9837\/revisions"}],"predecessor-version":[{"id":9851,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9837\/revisions\/9851"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/9835"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9837"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9837"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9837"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}