{"id":9715,"date":"2017-04-26T10:14:29","date_gmt":"2017-04-26T02:14:29","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9715"},"modified":"2023-08-25T17:48:15","modified_gmt":"2023-08-25T09:48:15","slug":"desalt%e8%84%ab%e9%b9%bd%e7%ae%a1%e6%9f%b1-buffer-exchange-and-desalting-of-protein-samples%e8%b2%a8%e8%99%9f6564-25","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9715","title":{"rendered":"Desalt\/\u812b\u9e7d\u7ba1\u67f1-7kD| buffer exchange and desalting of protein samples\u8ca8\u865f6564-25(ABCAM ab288149)"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=9715\"><strong>EZ-Desalt<\/strong><strong>\u812b\u9e7d<\/strong><strong>\u7ba1<\/strong><strong>\u67f1<\/strong><strong>Spin column desalt <em>|<\/em><\/strong> <strong>\u8ca8\u865f<\/strong><strong>6564-25(ABCAM ab288149)<\/strong><\/a><\/span><\/p>\n<p><em>Columns with pre-swollen Sephadex G-25 beads for buffer exchange and desalting of protein samples.<\/em><\/p>\n<p><strong>\u7522\u54c1\u4ecb\u7d39<\/strong><strong>:<\/strong> BioVision(ABCAM) \u7684EZ-Desalt\u2122\u65cb\u8f49\u812b\u9e7d\u67f1\u63d0\u4f9b\u4e86\u4e00\u500b\u65b9\u4fbf\u7684\u9078\u64c7\uff0c\u53ef\u4ee5\u5feb\u901f\u812b\u9e7d(Quickly desalt)\uff0c\u7de9\u885d\u4ea4\u63db(buffer-exchange)\u6216\u6e05\u7406(clean-up)\u6eb6\u6db2\u4e2d\u7684\u86cb\u767d\u8cea\u6a23\u54c1\u3002\u6bcf\u500b\u53ef\u68c4\u7528\u7684\u5373\u7528\u578b\u65cb\u8f49\u812b\u9e7d\u67f1\u5305\u542b0.5ml\u9810\u81a8\u8139\u7684Sephadex G-25\u73e0\u7c92\uff0c\u53ef\u6709\u6548\u5730\u5c07\u86cb\u767d\u8cea\uff08\u6216\u5176\u4ed6\u9ad8\u5206\u5b50\u91cf\u7d44\u5206\uff09\u8207\u5c0f\u5206\u5b50\uff08\u9e7d\u548c\u5176\u4ed6\u5c0f\u5206\u5b50\u5982LMW\u5206\u96e2\uff09\u5206\u96e2 \u67d3\u6599\u7b49\uff09\u3002\u5927\u5206\u5b50\u592a\u5927\u800c\u4e0d\u80fd\u9032\u5165\u73e0\u7684\u5b54\uff0c\u4e26\u4e14\u5f9eSephadex\u57fa\u8cea\u4e2d\u6392\u9664\uff0c\u4e26\u4e14\u5728\u67f1\u7684\u7a7a\u9699\u9ad4\u7a4d\u4e4b\u5f8c\u7acb\u5373\u88ab\u6d17\u812b\uff0c\u800c\u5c0f\u5206\u5b50\u88ab\u6a39\u8102\u4fdd\u7559\uff0c\u622a\u7559\u5927\u5c0f\u70ba\u7d047kDa\u3002 \u6574\u500b\u904e\u7a0b\u9700\u8981\u5e7e\u5206\u9418\u3002 \u4e26\u5be6\u73fe\u4e86\u512a\u7570\u7684\u86cb\u767d\u8cea\u56de\u6536\uff08&gt; 90\uff05\uff09\uff0c\u6709\u6548\u53bb\u9664\u4e86\u9e7d\u6216\u4e0d\u9700\u8981\u7684\u5c0f\u5206\u5b50\u3002<\/p>\n<p>\u8a72\u8272\u8b5c\u67f1\u53ef\u7528\u65bc 2.0 ml \u548c 1.5 ml \u96e2\u5fc3\u7ba1\u7684\u64fa\u6876\u6216\u56fa\u5b9a\u89d2\u8f49\u5b50\uff0c\u8f49\u901f\u70ba\u96e2\u5fc3\u529b 1,500 x g<\/p>\n<table style=\"height: 115px;\" width=\"912\">\n<tbody>\n<tr>\n<td><strong>Size<\/strong><\/td>\n<td>25 ea<\/td>\n<\/tr>\n<tr>\n<td><strong>SKU+Size<\/strong><\/td>\n<td>6564-25(ABCAM ab288149)<\/td>\n<\/tr>\n<tr>\n<td><strong>Highlights<\/strong><\/td>\n<td>\u2022 Quickly desalt, buffer-exchange, or clean-up protein samples in solution with exceptional (&gt;90%) recovery rate.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Pore Sizes for Ultrafiltration, Microfiltration, Dyalysis, and Macrofiltration at Spectrum Labs<\/p>\n<p><img decoding=\"async\" class=\"cte\" title=\"Click for larger image\" src=\"http:\/\/spectrumlabs.com\/mixed\/PoreSize.gif\" alt=\"Membrane pore size chart.\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>EZ-Desalt\u812b\u9e7d\u7ba1\u67f1Spin column desalt | \u8ca8\u865f6564-25(ABCAM ab288 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":8690,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[105,134,179],"tags":[3788],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9715"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9715"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9715\/revisions"}],"predecessor-version":[{"id":45392,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9715\/revisions\/45392"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8690"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9715"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9715"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9715"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}