{"id":9712,"date":"2017-04-25T12:20:51","date_gmt":"2017-04-25T04:20:51","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9712"},"modified":"2022-07-22T13:33:06","modified_gmt":"2022-07-22T05:33:06","slug":"%e7%b4%b0%e8%83%9e%e7%94%98%e6%b2%b9%e5%9f%ba%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bd%9c-glycerol-cell-based-assay-kit%e8%b2%a8%e8%99%9fk977","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9712","title":{"rendered":"\u7d30\u80de\u7518\u6cb9\u57fa\u6e2c\u5b9a\u8a66\u5291\u76d2\uff5c Glycerol Cell-Based Assay Kit\u8ca8\u865fK977"},"content":{"rendered":"

\u00a0Glycerol Cell-Based Assay Kit<\/strong>\u7d30\u80de\u7518\u6cb9\u57fa\u6e2c\u5b9a\u8a66\u5291\u76d2\uff5c\u8ca8\u865fK977\uff0d100<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Metabolic diseases\u4ee3\u8b1d\u75be\u75c5\u5982type II diabetes\u00a0II\u578b\u7cd6\u5c3f\u75c5\u548cfatty liver\u00a0\u8102\u80aa\u809d\u5728\u8001\u9f61\u5316\u4eba\u7fa4\u4e2d\u8b8a\u5f97\u8d8a\u4f86\u8d8a\u666e\u904d\uff0c\u5c0d\u91ab\u7642\u9ad4\u7cfb\u8ca0\u64d4\u6c89\u91cd\u3002\u9019\u7a2e\u75be\u75c5\u570d\u7e5e\u8eab\u9ad4\u63a7\u5236\u00a0triglyceride (lipid) metabolism\u7518\u6cb9\u4e09\u916f\uff08\u8102\u8cea\uff09\u4ee3\u8b1d\u7684\u80fd\u529b\u3002\u8af8\u5982\u00a0fasting\u7981\u98df\u7684\u4e8b\u4ef6\u53ef\u5c0e\u81f4Cell Autophagy\u7d30\u80de\u81ea\u566c\uff0c\u5c0d\u61c9\u65bclipid droplets\u8102\u6ef4\u7684\u53bb\u9664\u3002\u8a31\u591a\u75c5\u7406\u5b78\u53ef\u80fd\u5c0e\u81f4Steatosis\u8102\u80aa\u8b8a\u6027\uff0c\u5c0e\u81f4triacylglyceride buildup\u00a0\u4e09\u91af\u57fa\u7518\u6cb9\u916f\u7a4d\u7d2f\u548cfree fatty acids\u91cb\u653e\u6e38\u96e2\u8102\u80aa\u9178\u548cfree glycerol\u6e38\u96e2\u7518\u6cb9\u3002Glycerol molecules\u00a0\u7518\u6cb9\u5206\u5b50\u7136\u5f8c\u53ef\u4ee5\u88ab\u00a0liver\u00a0\u809d\u81df\u6216\u00a0kidney\u814e\u81df\u91cd\u65b0\u5438\u6536\uff0c\u4e26\u4e14\u53ef\u4ee5\u901a\u904e\u8af8\u5982\u00a0glycolysis\u00a0\u7cd6\u9175\u89e3\u548cgluconeogenesis\u7cd6\u7570\u751f\u4e4b\u985e\u7684\u9014\u5f91\u88ab\u4ee3\u8b1d\u4ee5\u91cd\u65b0\u9032\u5165\u80fd\u91cf\u5132\u5b58\u5668\u3002\u56e0\u6b64\uff0c\u7518\u6cb9\u6fc3\u5ea6\u53ef\u7528\u4f5c\u00a0lipid metabolism\u8102\u8cea\u4ee3\u8b1d\u548cfat mobilizationfat mobilizationfat mobilization\u8102\u80aa\u4e4b\u6e2c\u91cf\u6307\u6a19\u3002 BioVision\u7684\u7518\u6cb9 (Cell-Base) \u7684\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u3001\u9748\u654f\u3001\u76f4\u89c0\u7684\u6e2c\u5b9a\u65b9\u6cd5\uff0c\u7528\u65bc\u6e2c\u5b9a\u5404\u7a2e\u7d30\u80de\u548c\u7d44\u7e54\u57f9\u990a\u6a23\u54c1\u4e2d\u7518\u6cb9\u6fc3\u5ea6\u3002\u8a72\u6e2c\u5b9a\u6cd5\u4f7f\u7528\u7518\u6cb9\u7279\u7570\u6027\u9176\u4fc3\u53cd\u61c9\uff0c\u5728\u7518\u6cb9\u5b58\u5728\u4e0b\u5c07\u63a2\u91dd\u8f49\u5316\u6210\u87a2\u5149\u7522\u7269\u3002\u7518\u6cb9\u6c34\u5e73\u53ef\u4ee5\u6aa2\u6e2c\u5230\u4f4e\u81f3\u6bcf\u5b5450 pmol\uff0c\u6216\u57f9\u990a\u57fa\u4e2d\u76841.0\u03bcM\u3002Chloroquine\u00a0\u6c2f\u55b9\uff08\u5305\u62ec\u5728\u8a66\u5291\u76d2\u4e2d\u4f5c\u70ba\u5c0d\u7167\uff09\u8a98\u5c0esteatosis\u8102\u80aa\u8b8a\u6027\u4e26\u963b\u65b7\u00a0autophagy\u81ea\u566c\uff0c\u5f9e\u800c\u5c0e\u81f4\u8102\u6ef4\u7684\u7a4d\u805a\u548c\u6e38\u96e2\u7518\u6cb9\u6fc3\u5ea6\u7684\u589e\u52a0\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n
Size<\/td>\n100 assays<\/td>\n<\/tr>\n
SKU+Size<\/td>\nK977-100<\/td>\n<\/tr>\n
Detection Method<\/td>\nFluorescence (Ex\/Em 535\/587 nm)<\/td>\n<\/tr>\n
Applications<\/td>\nThe kit can detect 100 pmol of glycerol in mammalian cell cultures.<\/td>\n<\/tr>\n
Features & Benefits<\/td>\n\u2022 Simple procedure; takes ~ 2 hours \u2022 Fast and convenient \u2022 The kit provides an accurate assay for measuring free glycerol in cell-based assays<\/td>\n<\/tr>\n
Kit Components<\/td>\n\u2022 Glycerol Assay Buffer \u2022 Glycerol Probe (in DMSO) \u2022 Glycerol Enzyme Mix \u2022 Glycerol Standard (100 mM) \u2022 Chloroquine (5 mM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1<\/strong>: <\/strong>\u3010\u5b9a\u91cf\u5206\u6790\u5546\u54c1\u3011<\/strong> \u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790\u5957\u7d44<\/a> | CETP\u81bd\u56fa\u9187\u916f\u8f49\u904b\u86cb\u767dELISA<\/a> | Irisin\u7af6\u722d\u578bELISA<\/a> | Free Fatty Acid\u5448\u8272\u87a2\u5149\u5206\u6790<\/a> | Triglyceride\u4e09\u9178\u7518\u6cb9\u916f\u5b9a\u91cf\u5206\u6790<\/a> \u3010\u67d3\u8272\u5546\u54c1\u3011<\/strong> Lipid\u8102\u8cea\u67d3\u8272\u5957\u7d44<\/a> | \u81bd\u56fa\u9187\u67d3\u8272\u5957\u7d44<\/a> \u3010\u8403\u53d6\u5546\u54c1\u3011<\/strong>\u8102\u80aa\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6<\/a><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u00a0Glycerol Cell-Based Assay Kit\u7d30\u80de\u7518\u6cb9\u57fa\u6e2c\u5b9a\u8a66\u5291\u76d2\uff5c\u8ca8\u865fK977\uff0d100 \u7522\u54c1\u4ecb […]<\/p>\n","protected":false},"author":3,"featured_media":6324,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[466,124],"tags":[3789],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9712"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9712"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9712\/revisions"}],"predecessor-version":[{"id":18607,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9712\/revisions\/18607"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6324"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9712"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9712"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9712"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}