{"id":9707,"date":"2017-04-25T09:04:39","date_gmt":"2017-04-25T01:04:39","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9707"},"modified":"2022-11-01T14:44:42","modified_gmt":"2022-11-01T06:44:42","slug":"anoikis%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bd%9c%e8%b2%a8%e8%99%9fk694%ef%bc%8d100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9707","title":{"rendered":"\u5931\u5de2\u51cb\u4ea1(Anoikis) \u6e2c\u5b9a\u8a66\u5291\u76d2\uff5cAnoikis Assay Kit\u8ca8\u865fK694-100 (abcam ab284938)"},"content":{"rendered":"

\u7d30\u80de\u57fa\u790e\u7814\u7a76<\/strong> \uff5c<\/strong>Cell-base assay<\/strong><\/p>\n

Anoikis Assay Kit<\/p>\n

\u5546\u54c1:\u00a0Anoikis\u6e2c\u5b9a\u8a66\u5291\u76d2\uff5c\u8ca8\u865f: K694-100<\/a>\u00a0(ab284938)<\/span><\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u5931\u5de2\u51cb\u4ea1(Anoikis)\u00a0\u662f\u7531\u6b63\u5e38\u7d30\u80de\u4e0d\u80fd\u9644\u8457\u65bc\u7d30\u80de\u5916\u57fa\u8cea\uff08ECM\uff09\u8a98\u5c0e\u7684\u7d30\u80de\u51cb\u4ea1\u5f62\u5f0f\u3002 \u4e0d\u6b63\u78ba\u7684\u9644\u8457\u6216\u6c92\u6709ECM\u9644\u8457\u6703\u5f71\u97ff\u6b63\u5e38\u7d30\u80de\u7684\u7d30\u80de\u751f\u9577\u548c\u5206\u5316\u3002 \u7136\u800c\uff0c\u7814\u7a76\u5df2\u7d93\u78ba\u5b9a\uff0c\u8f49\u79fb\u6027\u7d30\u80de\u53ef\u4ee5\u7d93\u6b77\u5931\u5de2\u51cb\u4ea1(Anoikis)\u00a0\u800c\u4e0d\u5f71\u97ff\u5176\u9077\u79fb\u548c\u4fb5\u5165\u5468\u570d\u6216\u9060\u7aef\u5668\u5b98\u7684\u80fd\u529b\u3002 BioVision\u7684\u5931\u5de2\u51cb\u4ea1(Anoikis)\u00a0\u6e2c\u5b9a\u8a66\u5291\u76d2\u53ef\u4ee5\u87a2\u5149\uff08Calcein AM\uff09\u8a66\u5291\u548c\u6a21\u64ec\u5931\u5de2\u51cb\u4ea1(Anoikis)\u00a0\u689d\u4ef6\u7684\u5c08\u6709\u5857\u5c64\u5ba4\u7684\u6d3b\u7d30\u80de\u3002Biovision\u6e2c\u5b9a\u662f\u6613\u65bc\u4f7f\u7528\uff0c\u5177\u9748\u654f\u611f\u548c\u53ef\u4f7f\u7528\u87a2\u5149\u5100 (\u00a0Ex\/Em = 485\/530 nm)\u6aa2\u6e2c\u3002<\/p>\n

\"\"<\/p>\n

\u57161\uff1a\u5931\u5de2\u51cb\u4ea1(Anoikis)\u00a0\u6aa2\u6e2c\uff1a\u7d30\u80de\u70ba3T3-NIH\u548cHela\u3002 3T3\u7d30\u80de\u5728Anoikis Chamber\uff08coated\uff09\u4e2d\u7d93\u6b77Anoikis\uff08\u51cb\u4ea1\uff09\uff0c\u800c\u764c\u7d30\u80de\u514b\u670d\u4e86\u5931\u5de2\u51cb\u4ea1(Anoikis)\u00a0\uff0c\u4e26\u80fd\u5920\u5728\u4e0d\u5b58\u5728Anchorage\u7684\u60c5\u6cc1\u4e0b\u751f\u9577\uff08Chamber Control, Uncoated\uff09\u3002\u6c92\u8b8a \u5728\u672a\u5857\u8986\u7684\u5e73\u677f\u4e2d\u89c0\u5bdf\u5230\u7d30\u80de\u751f\u9577\u6c92\u8b8a\u3002<\/p>\n\n\n\n\n\n\n\n\n\n
Size<\/td>\n100 assays<\/td>\n<\/tr>\n
SKU+Size<\/td>\nK694-100<\/td>\n<\/tr>\n
Detection Method<\/td>\nFluorescence (Ex\/Em = 485\/530 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/td>\nMammalian cells<\/td>\n<\/tr>\n
Applications<\/td>\nMeasure cell anoikis in response to stimuli. Screen, study, or characterize compounds that influence Anoikis. HTP adaptable<\/td>\n<\/tr>\n
Features & Benefits<\/td>\n\u2022 Highly sensitive fluorometric method to measure cell Anoikis in response to a variety of biochemical stimuli \u2022 Simple & High throughput-adaptable \u2022 Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect Anoikis<\/td>\n<\/tr>\n
Kit Components<\/td>\n\u2022 Assay Buffer \u2022 Anoikis Chamber \u2022 Chamber Control (Uncoated) \u2022 Calcein AM Dye<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5176\u5b83\u5546\u54c1<\/strong>: <\/strong>\u3010\u5b9a\u91cf\u5206\u6790\u5546\u54c1\u3011<\/strong> \u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790\u5957\u7d44<\/a> | CETP\u81bd\u56fa\u9187\u916f\u8f49\u904b\u86cb\u767dELISA<\/a> | Irisin\u7af6\u722d\u578bELISA<\/a> | Free Fatty Acid\u5448\u8272\u87a2\u5149\u5206\u6790<\/a> | Triglyceride\u4e09\u9178\u7518\u6cb9\u916f\u5b9a\u91cf\u5206\u6790<\/a> \u3010\u67d3\u8272\u5546\u54c1\u3011<\/strong> Lipid\u8102\u8cea\u67d3\u8272\u5957\u7d44<\/a> | \u81bd\u56fa\u9187\u67d3\u8272\u5957\u7d44<\/a> \u3010\u8403\u53d6\u5546\u54c1\u3011<\/strong>\u8102\u80aa\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6<\/a><\/p>\n

\u64cd\u4f5c\u6d41\u7a0b (\u6a19\u6e96\u4f9d\u539f\u5ee0\u516c\u544a)\u00a0<\/a><\/span><\/p>\n

1.\u7d30\u80de\u751f\u9577\uff1a\u5c07\u6240\u9700\u7d30\u80de\u5efa\u8b70\u7684\u57f9\u990a\u57fa\u548c\u57f9\u990a\u689d\u4ef6\u4e0b\u57f9\u990a\u81f3\u7d0480\uff05\u3002\u6536\u7a6b\u7d30\u80de\u4e26\u4ee51,000\u96e2\u5fc3x g\uff0c\u6301\u7e8c5\u5206\u9418\u3002\u6c89\u6fb1\u7d30\u80de\u3002\u5c07\u7d30\u80de\u6c89\u6fb1\u91cd\u61f8\u65bc\u57f9\u990a\u57fa\u4e2d\uff0c\u4e26\u4f7f\u7528\u8840\u7403\u7d30\u80de\u8a08\u6578\u5668\u6216\u81ea\u52d5\u7d30\u80de\u8a08\u6578\u5668\uff0c\u8a08\u7b97\u7d30\u80de\u6578\u91cf\u3002\u4ee51 x 106 cells\/ml (\u57f9\u990a\u57fa)\u3002\u7121\u83cc\u64cd\u4f5c\u53f0(laminar flow hood)\u4e0b\u6253\u958bAnoikis Chamber\u548cChamber Control (Uncoated) \uff1b\u4ee5100 \u00b5l\u7121\u83cc\u6e2c\u5b9a\u7de9\u885d\u6db2(sterile Assay Buffer) \u6d17\u6ecc\u5169\u6b21\u3002\u52a0\u5165100 \u00b5l\u7d30\u80de\u61f8\u6db2 (cell suspensions)\u81f3Anoikis Chamber \u53ca the Chamber Control (Uncoated)<\/p>\n

\u6ce8\u610f\uff1a\u9069\u7576\u7684incubation time\u53d6\u6c7a\u65bc\u6240\u7528\u7684\u55ae\u500b\u7d30\u80de\u985e\u578b\u548c\u7d30\u80de\u6fc3\u5ea6\u3002\u56e0\u6b64\uff0c\u5efa\u8b70\uff0c\u78ba\u5b9a\u6bcf\u500b\u5be6\u9a57\u7684\u6700\u4f73incubation time\u3002<\/p>\n

2.\u8655\u7406\uff1a\u7528Anoikis enhancing\u6216inhibiting reagents\u8655\u7406\u7d30\u80de\u3002\u5c0d\u65bcbackground control\uff0c\u7528vehicle\u8655\u7406\u7d30\u80de\u3002\u57f9\u990a\u7d30\u80de24-72\u5c0f\u6642\u3002\u572837\u00baC\u548c5\uff05\u7684\u4e8c\u6c27\u5316\u78b3\u3002incubation time\u548c\u57f9\u990a\u689d\u4ef6\u53d6\u6c7a\u65bccell line\uff0c\u9700\u8981\u5c0d\u5176\u9032\u884c\u8abf\u6574\u3002<\/p>\n

3.\u6578\u64da\u6536\u96c6\u548c\u5206\u6790\uff1a\u7d93\u904e\u8a98\u5c0e\u5291\/\u6291\u88fd\u5291\u9810\u671f\u7684\u6eab\u80b2\u5f8c\uff0c\u5c0f\u5fc3\u53d6\u4e0bplate cover\u3002\u52a0\u51652 \u00b5l\u9223\u9ec3\u7da0\u7d20AM\u5c0796\u5b54\u6297\u56fa\u5b9a\u677f\u6216\u63a7\u5236\u677f\u7684\u6bcf\u500b\u5b54\u4e2d\u7684\u67d3\u6599\u67d3\u8272\u6aa2\u6e2c\u5230\u3002\u5b75\u80b2\u677f30-60\u5206\u9418\u3002\u572837\u00baC\u4e0b\u3002\u5fae\u89c0\u76e3\u63a7\u7d30\u80de\u9223\u9ec3\u7da0\u7d20AM\uff08\u7da0\u8272\u901a\u9053\uff09\u87a2\u5149\u7684\u5b58\u5728\u3002\u87a2\u5149\u53ef\u4ee5\u7528\u87a2\u5149\u5100\u5668\u6e2c\u91cf(Ex: 485 nm and Em: 530 nm)\u3002\u5f9e\u8655\u7406\u904e\u7684\u6a23\u54c1\u4e2d\u6e1b\u53bb\u80cc\u666f\u5c0d\u7167(background control)\u3002<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u7d30\u80de\u57fa\u790e\u7814\u7a76 \uff5cCell-base assay Anoikis Assay Kit \u5546\u54c1:\u00a0Anoikis\u6e2c\u5b9a […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[84,126,434],"tags":[3791],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9707"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9707"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9707\/revisions"}],"predecessor-version":[{"id":35737,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9707\/revisions\/35737"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9707"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9707"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9707"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}