{"id":9640,"date":"2017-04-22T11:20:45","date_gmt":"2017-04-22T03:20:45","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9640"},"modified":"2025-06-27T16:16:04","modified_gmt":"2025-06-27T08:16:04","slug":"%e5%90%9e%e5%99%ac%e8%a9%a6%e9%a9%97%e7%9b%92%ef%bc%88red-e-coli%ef%bc%89%ef%bd%9cezcellphagocytosis-assay-kit-%e8%b2%a8%e8%99%9f-k964-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9640","title":{"rendered":"\u541e\u566c\u8a66\u9a57\u76d2 (Red E. coli)\uff5cEZCell\u2122Phagocytosis Assay Kit \u8ca8\u865f: K964-100 (ABCAM ab235901)"},"content":{"rendered":"

\u541e\u566c\u8a66\u9a57\u76d2\uff5c<\/strong>EZCell\u2122<\/strong>\u7cfb\u5217\u5546\u54c1<\/strong><\/span><\/span><\/p>\n

\u5546\u54c1<\/strong>: <\/strong>EZCell\u2122\u541e\u566c\u8a66\u9a57\u76d2\uff08Red E. coli\uff09\uff5c\u8ca8\u865f: K964-100<\/a>\u00a0\u00a0(ABCAM ab235901)<\/span><\/p>\n

\u54fa\u4e73\u52d5\u7269\u541e\u566c\u4f5c\u7528\u662f\u9632\u6b62\u5165\u4fb5\u75c5\u539f\u9ad4\u7684\u91cd\u8981\u4e00\u7dda\u9632\u79a6\u6a5f\u5236\u3002\u9023\u7e8c\u6e05\u9664\u6b7b\u4ea1\u7d30\u80de\uff0c\u7d44\u7e54\u91cd\u5851\u4ee5\u53ca\u7372\u53d6\u67d0\u4e9b\u7d30\u80de\u7684\u71df\u990a\u7d20\u4e5f\u662f\u5fc5\u4e0d\u53ef\u5c11\u7684\u3002\u541e\u566c\u4f5c\u7528\u662f\u901a\u904e\u7d30\u80de\u8868\u9762\u53d7\u9ad4\u8b58\u5225\u548c\u7d50\u5408\u5916\u4f86\u9846\u7c92\u800c\u5f15\u767c\u7684\u7279\u6b8a\u5f62\u5f0f\u7684\u5167\u541e\u4f5c\u7528\uff0c\u5176\u541e\u566c\u548c\u5f62\u6210\u541e\u566c\u9ad4\u3002\u6210\u719f\u7684\u541e\u566c\u9ad4\u8f49\u5316\u6210\u901a\u904e\u9176\u548c\u6709\u6bd2\u904e\u6c27\u5316\u7269\u7834\u58de\u75c5\u539f\u9ad4\u7684\u541e\u566c\u9ad4\u3002\u5927\u8178\u687f\u83cc\u548c\u5176\u4ed6\u7d30\u83cc\u83cc\u682a\u901a\u5e38\u7528\u4f5c\u541e\u566c\u8a66\u9a57\u4e2d\u7684\u75c5\u539f\u9ad4\u3002 BioVision\u7684EZCell\u2122\u541e\u566c\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08Red E. coli\uff09\u5229\u7528\u71b1\u6ec5\u6d3b\u7684\u87a2\u5149\u9810\u5148\u6a19\u8a18\u7684\u5927\u8178\u687f\u83cc\u9846\u7c92\u4f5c\u70ba\u5feb\u901f\u6e96\u78ba\u6aa2\u6e2c\u548c\u5b9a\u91cf\u87a2\u5149\u986f\u5fae\u93e1\uff0c\u5206\u5149\u5149\u5ea6\u8a08\u6216\u6d41\u5f0f\u7d30\u80de\u8853\u7684\u9ad4\u5916\u541e\u566c\u4f5c\u7528\u7684\u5de5\u5177\u3002\u8a72\u8a66\u5291\u76d2\u70ba\u6fc0\u6d3b\u5291\u548c\/\u6216\u541e\u566c\u4f5c\u7528\u548cToll\u6a23\u53d7\u9ad4\uff08TLR\uff09\u914d\u9ad4\u6291\u5236\u5291\u63d0\u4f9b\u4e86\u5f37\u5927\u7684\u7be9\u9078\u7cfb\u7d71\u3002<\/p>\n

\"Functional<\/p>\n

\n
\n

Inhibition of phagocytosis. J774 macrophages were seeded overnight at 5 x 105<\/sup>\u00a0of viable cells\/well. The next day the cells were pretreated with 20 \u03bcM Cytochalasin D for 1 hour at 37\u00b0C prior to addition of 5 \u03bcL of\u00a0E. coli<\/em>\u00a0particles. Phagocytosis was conducted for 2 hours and the amount of engulfed\u00a0E. coli<\/em>\u00a0was determined as described in the Assay Protocol. Panel A and B: images of nontreated cells. Panel C and D: treatment with Cytochalasin D.<\/p>\n<\/div>\n<\/div>\n

\"Flow<\/p>\n

\n
\n

J774 macrophages were seeded overnight at 5 x 105<\/sup>\u00a0of viable cells\/well. The next day the cells were pretreated with 20 \u03bcM Cytochalasin D for 1 hour at 37\u00b0C prior to addition of 5 \u03bcL of\u00a0E. coli<\/em>\u00a0particles. Phagocytosis was conducted for 2 hours and the amount of engulfed\u00a0E. coli<\/em>\u00a0was determined as described in the Assay Protocol. Blue line: untreated control cells; red line: macrophages with engulfed E. coli particles; green line: inhibition of phagocytosis by Cytochalasin D.<\/p>\n<\/div>\n<\/div>\n

\"Functional<\/p>\n

\n
E. coli<\/em>\u00a0Standard curve.<\/div>\n<\/div>\n

Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. E. coli and other bacterial strains are often used as a pathogen in phagocytosis assays. BioVision\u2019s EZCell\u2122 Phagocytosis Assay Kit (Red E. coli) utilizes heat-killed, fluorescently pre-labeled E. coli particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. The kit provides a robust screening system for activators and\/or inhibitors of phagocytosis and Toll-like receptors (TLR) ligands<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n
Size<\/td>\n100 assays<\/td>\n<\/tr>\n
SKU+Size<\/td>\nK964-100<\/td>\n<\/tr>\n
Detection Method<\/td>\nFluorescence (Ex\/Em = 540\/570 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/td>\nRapid detection, quantification and validation of phagocytosis in convenient 96-well format – Tracking ligand internalization and screening for effectors of phagocytosis<\/td>\n<\/tr>\n
Features & Benefits<\/td>\n\u2022 Simple & Rapid Protocol \u2022 Convenient: Non-Radioactive, no special handling or disposal required \u2022 High-Throughput \u2022 Accurate: reproducible results with low intra & inter assay variability<\/td>\n<\/tr>\n
Kit Components<\/td>\n\u2022 Phagocytosis Assay Buffer \u2022 Red E. Coli \u2022 10X Quenching Solution<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u541e\u566c\u8a66\u9a57\u76d2\uff5cEZCell\u2122\u7cfb\u5217\u5546\u54c1 \u5546\u54c1: EZCell\u2122\u541e\u566c\u8a66\u9a57\u76d2\uff08Red E. coli\uff09\uff5c\u8ca8\u865f: K96 […]<\/p>\n","protected":false},"author":3,"featured_media":8690,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261,191],"tags":[3807],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9640"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9640"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9640\/revisions"}],"predecessor-version":[{"id":51729,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9640\/revisions\/51729"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8690"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9640"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9640"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9640"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}