{"id":9450,"date":"2017-04-13T15:42:44","date_gmt":"2017-04-13T07:42:44","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9450"},"modified":"2022-07-22T14:28:09","modified_gmt":"2022-07-22T06:28:09","slug":"9450","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9450","title":{"rendered":"Minute\u2122\u8449\u7da0\u9ad4\u5206\u96e2\u8a66\u5291\u76d2| Detergent-Free Plant Protein Extraction\u8ca8\u865fCP-011"},"content":{"rendered":"

\u7522\u54c1\u540d\u7a31<\/strong>: Minute\u2122<\/a><\/span><\/strong>\u8449\u7da0\u9ad4<\/strong>\u5206\u96e2<\/strong>\u8a66\u5291\u76d2<\/strong>|<\/strong> Detergent-Free Plant Protein Extraction<\/strong>\u8ca8\u865f<\/strong>CP-011<\/strong><\/a><\/span><\/p>\n

Invent Biotechnologies’Minute\u2122 \u8449\u7da0\u9ad4\u5206\u96e2\u8a66\u5291\u76d2\u7531\u512a\u5316\u7684\u8449\u7da0\u9ad4\u5206\u96e2\u7de9\u885d\u6db2\u548c\u542b2.0ml\u6536\u96c6\u7ba1\u7684\u6ffe\u7b52\u7d44\u6210\u3002\u8a72\u8a66\u5291\u76d2\u65e8\u5728\u5f9e\u65b0\u9bae\u690d\u7269\u7d44\u7e54\uff08\u8449\uff0c\u7a2e\u5b50\u548c\u8edf\u8396\u7b49\uff09\u5feb\u901f\u63d0\u53d6\u5b8c\u6574\u7684\u8449\u7da0\u9ad4\u3002\u7531\u65bc\u4f7f\u7528\u5177\u6709\u9810\u5b9a\u5b54\u5f91\u548c\u539a\u5ea6\u7684\u6ffe\u82af\uff0c\u53ef\u4ee5\u5feb\u901f\u5f9e\u65b0\u9bae\u690d\u7269\u7d44\u7e54\u4e2d\u5206\u96e2\u51fa\u5b8c\u6574\u7684\u8449\u7da0\u9ad4\u3002\u8207\u9700\u89811-10 + g\u7d44\u7e54\u9032\u884c\u8449\u7da0\u9ad4\u5206\u96e2\u7684\u8a31\u591a\u5176\u4ed6\u65b9\u6cd5\u4e0d\u540c\uff0c\u8a72\u8a66\u5291\u76d2\u53ef\u4ee5\u5728\u4e0d\u52305\u5206\u9418\u7684\u6642\u9593\u5167\u5f9e5-200 mg\u65b0\u9bae\u690d\u7269\u8449\u4e2d\u5feb\u901f\u7372\u5f971 X 106<\/sup>\u81f31 X 107<\/sup>\u5b8c\u6574\u7684\u8449\u7da0\u9ad4\uff08> 90\uff05\u5b8c\u6574\uff09\u3002<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u61c9\u7528: <\/strong><\/p>\n

MinuteTM\u8449\u7da0\u9ad4\u5206\u96e2\u8a66\u5291\u76d2\u65e8\u5728\u65e8\u5728\u5feb\u901f\u5f9e\u65b0\u9bae\u690d\u7269\u7d44\u7e54\u7684\u5c0f\u6a23\u672c\uff08100-200 mg\uff09\u4e2d\u5206\u96e2\u51fa\u5b8c\u6574\u7684\u8449\u7da0\u9ad4\uff0c\u7528\u65bc\u751f\u5316\u61c9\u7528\u5206\u6790\uff0cSDS-PAGE\uff0c\u514d\u75ab\u5370\u8de1\uff0c\u9176\u6e2c\u5b9a\u7b49\u3002\u5206\u96e2\u7684\u8449\u7da0\u9ad4\u53ef\u7528\u4f5c\u8d77\u59cb\u539f\u6599\u7528\u65bc\u5206\u5b50\u751f\u7269\u5b78\u7814\u7a76\u7684\u7d14\u5316RNA\u548cDNA\u7684\u6750\u6599\u3002<\/p>\n

Minute\u2122<\/strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300e<\/strong>Column Filter<\/strong>\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong>\u00a0<\/strong>\u4e00<\/strong> \u5206\u9418\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<\/strong>vortex<\/em><\/strong>\u3001<\/em><\/strong>transfer<\/em><\/strong>\u3001<\/em><\/strong>spin <\/em><\/strong>\u3002<\/em><\/strong>Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\uff1b\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\uff0c\u7d30\u80de\u5206\u96e2\u548c\u819c\u86cb\u767d\u5206\u96e2\uff0c\u53ea\u9700\u7c21\u55ae\u56db\u6b65\u9a5f\u5373\u53ef\u5b8c\u6210\u86cb\u767d\u8cea\u8403\u53d6\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n\n\n\n\n\n\n\n\n
Item<\/em><\/strong><\/td>\nAmount<\/em><\/strong><\/td>\nPart #<\/em><\/strong><\/td>\n<\/tr>\n
Buffer A (with 5g dissociation beads)<\/td>\n25 ml<\/td>\nCP-011-A<\/td>\n<\/tr>\n
Buffer B<\/td>\n25 ml<\/td>\nCP-011-B<\/td>\n<\/tr>\n
Protein Extraction Filter Cartridges<\/td>\n50<\/td>\nP-001<\/td>\n<\/tr>\n
Collection Tubes with Cap<\/td>\n50<\/td>\nP-002<\/td>\n<\/tr>\n
plastic rods<\/td>\n4<\/td>\nP-003<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat #<\/strong><\/td>\nDescription<\/strong><\/td>\nSize<\/strong><\/td>\n<\/tr>\n
SC-003<\/a><\/td>\nCytoplasmic & Nuclear Extraction Kits<\/a><\/td>\n50 Preps<\/td>\n<\/tr>\n
SM-005<\/a><\/td>\nPlasma Membrane Protein Isolation Kit<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
NE-013<\/a><\/td>\nNuclear Envelope Protein Extraction Kit<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
MP-007<\/a><\/td>\nMitochondria Isolation Kit For Mammalian Cells And Tissues<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
AF-023<\/a><\/td>\nAdipose Tissue Fractionation Kit<\/a><\/td>\n20 preps<\/td>\n<\/tr>\n
NI-024<\/a><\/td>\nDetergent-free nuclei isolation kit<\/a><\/td>\n20 preps<\/td>\n<\/tr>\n
YM-017<\/a><\/td>\nYeast Mitochondria Enrichment Kit<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
MM-018<\/a><\/td>\nPlant Microsomal Membrane Extraction Kit<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n
CP-011<\/a><\/td>\nChloroplast Isolation Kit<\/a><\/td>\n50 preps<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat. No<\/td>\nStarting Materials<\/td>\nStatus of Extracted Proteins<\/td>\nYield
\n(mg\/ml)<\/td>\n		Protocol\u00a0Time
\n(min)<\/td>\n<\/tr>\n
SD-001\/ SN-002<\/td>\nCultured Animal Cells and Tissues<\/td>\nDenatured\/Native<\/td>\n2-8<\/td>\n1-8<\/td>\n<\/tr>\n
SC-003<\/td>\nMammalian Cultured Cells\/Tissues<\/td>\nNative<\/td>\n1-3<\/td>\n6-8<\/td>\n<\/tr>\n
SN-006<\/td>\nCultured Animal Cells and Tissues<\/td>\nDenatured\/Native<\/td>\n1-4<\/td>\n5-8<\/td>\n<\/tr>\n
SM-005<\/td>\nMammalian Cultured Cells\/Tissues<\/td>\nNative Detergent\/ EDTA -Free<\/td>\n1-3<\/td>\n20-45<\/td>\n<\/tr>\n
MP-007<\/td>\nMammalian Cultured Cells\/Tissues<\/td>\nNative Detergent\/ EDTA-Free<\/td>\n1-3<\/td>\n25-30<\/td>\n<\/tr>\n
SD-008\/ SN-009<\/td>\nFresh Plant Tissues<\/td>\nDenatured\/Native<\/td>\n2-8<\/td>\n5-8<\/td>\n<\/tr>\n
SN-010<\/td>\nFresh Plant Tissues<\/td>\nNative<\/td>\n1-5<\/td>\n6-8<\/td>\n<\/tr>\n
CP-011<\/td>\nFresh Plant Tissues (5-200 mg\/sample)<\/td>\nChloroplast (>90%) Intact<\/td>\n1-4<\/td>\n5<\/td>\n<\/tr>\n
SB-004<\/td>\nCultured Bacterial Cells<\/td>\nDenatured<\/td>\n2-3<\/td>\n2-3<\/td>\n<\/tr>\n
NE-013<\/td>\nCultured cells or Cells from Tissues<\/td>\nNative<\/td>\n1-3<\/td>\n45<\/td>\n<\/tr>\n
HP-014<\/td>\nCultured cells or Cells from Tissues<\/td>\nDenatured<\/td>\n1-2.5<\/td>\n<10<\/td>\n<\/tr>\n
YT-015<\/td>\nCultured Microbes<\/td>\nDenatured\/Native<\/td>\n2-5<\/td>\n<10 min<\/td>\n<\/tr>\n
YD-016<\/td>\nCultured Microbes<\/td>\nDenatured\/Native<\/td>\n2-4<\/td>\n<10 min<\/td>\n<\/tr>\n
YM-017<\/td>\nCultured Yeast<\/td>\nNative<\/td>\n2-3<\/td>\n1h<\/td>\n<\/tr>\n
MM-018<\/td>\nPlant tissues<\/td>\nNative<\/td>\n2-3<\/td>\n1h<\/td>\n<\/tr>\n
PN-019<\/td>\nPAGE\/Agarose gels<\/td>\nDenatured\/Native<\/td>\nNA<\/td>\n10-20 min<\/td>\n<\/tr>\n
HD-021<\/td>\nKeratinized tissues<\/td>\nDenatured<\/td>\n1-2<\/td>\n5 min hand on<\/td>\n<\/tr>\n
AT-022<\/td>\nAdipose Tissues\/Adipocytes<\/td>\nNative\/Denatured<\/td>\n2-3<\/td>\n20 min<\/td>\n<\/tr>\n
AF-023<\/td>\nAdipose Tissues\/Adipocytes<\/td>\nNative<\/td>\n0.5-1<\/td>\n20 min<\/td>\n<\/tr>\n
FE-025<\/td>\nFFPE<\/td>\nDenatured<\/td>\n2-3<\/td>\n1h<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Sub-Cellular Structure Fractionation\/Isolation<\/strong><\/td>\n<\/tr>\n
Cat #<\/strong><\/td>\nDescription<\/strong><\/td>\nSize<\/strong><\/td>\n<\/td>\n<\/tr>\n
SC-003<\/a><\/td>\nCytoplasmic & Nuclear Extraction Kits<\/a><\/td>\n50 Preps<\/td>\n<\/td>\n<\/tr>\n
SM-005<\/a><\/td>\nPlasma Membrane Protein Isolation Kit<\/a><\/td>\n50 preps<\/td>\n<\/td>\n<\/tr>\n
NE-013<\/a><\/td>\nNuclear Envelope Protein Extraction Kit<\/a><\/td>\n50 preps<\/td>\n<\/td>\n<\/tr>\n
MP-007<\/a><\/td>\nMitochondria Isolation Kit For Mammalian Cells And Tissues<\/a><\/td>\n50 preps<\/td>\n<\/td>\n<\/tr>\n
AF-023<\/a><\/td>\nAdipose Tissue Fractionation Kit<\/a><\/td>\n20 preps<\/td>\n<\/td>\n<\/tr>\n
NI-024<\/a><\/td>\nDetergent-free nuclei isolation kit<\/a><\/td>\n20 preps<\/td>\n<\/td>\n<\/tr>\n
YM-017<\/a><\/td>\nYeast Mitochondria Enrichment Kit<\/a><\/td>\n50 preps<\/td>\n<\/td>\n<\/tr>\n
MM-018<\/a><\/td>\nPlant Microsomal Membrane Extraction Kit<\/a><\/td>\n50 preps<\/td>\n<\/td>\n<\/tr>\n
CP-011<\/a><\/td>\nChloroplast Isolation Kit<\/a><\/td>\n50 preps<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u7522\u54c1\u540d\u7a31: Minute\u2122\u8449\u7da0\u9ad4\u5206\u96e2\u8a66\u5291\u76d2| Detergent-Free Plant Protein Ext […]<\/p>\n","protected":false},"author":3,"featured_media":9451,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1,3],"tags":[3840],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9450"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9450"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9450\/revisions"}],"predecessor-version":[{"id":25108,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9450\/revisions\/25108"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/9451"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9450"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9450"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9450"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}