{"id":9405,"date":"2017-04-12T16:03:14","date_gmt":"2017-04-12T08:03:14","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9405"},"modified":"2022-10-29T15:40:56","modified_gmt":"2022-10-29T07:40:56","slug":"amylase%e6%b4%bb%e6%80%a7%e5%91%88%e8%89%b2%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-amylase-activity-colorimetric-assay-%e8%b2%a8%e8%99%9fk711-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9405","title":{"rendered":"Amylase\u6d3b\u6027\u5448\u8272\u5206\u6790 |Amylase Activity Colorimetric Assay \u8ca8\u865fK711-100 (abcam ab102523)"},"content":{"rendered":"

Amylase<\/em><\/strong>\u6d3b\u6027\u5448\u8272\u5206\u6790\u5957\u7d44<\/strong> | <\/strong>\u8ca8\u865fK711-100<\/strong><\/a>\u00a0(alpha-amylase activity)\u00a0\u00a0(abcam ab102523)<\/span><\/p>\n

Amylase Activity Colorimetric Assay Kit<\/em><\/strong><\/p>\n

\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\"\u539f\u7406\u8207\u77e5\u8b58\"<\/p>\n

\u6fb1\u7c89\u9176 (Amylases )\u662f\u5c07\u6fb1\u7c89\u5206\u89e3\u6210\u7cd6\u5206\u5b50 (\u00a0sugar molecules) \u7684\u9176 (enzymes )\u3002 \u03b1-\u6fb1\u7c89\u9176 (\u00a0\u03b1-amylase) \u662f\u4eba\u548c\u5176\u4ed6\u54fa\u4e73\u52d5\u7269\u4e2d\u767c\u73fe\u7684\u6fb1\u7c89\u9176\u7684\u4e3b\u8981\u5f62\u5f0f\uff0c\u4ee5\u53ca\u5b58\u5728\u65bc\u7a2e\u5b50 (seeds) \u6216\u771f\u83cc (\u00a0fungi ) \uff08\u4f8b\u5982\u9eb5\u5305\u9175\u6bcd\uff09\u4e2d\u7684\u9176\u3002 \u03b1-\u6fb1\u7c89\u9176\u662f\u9223\u91d1\u5c6c\u9176 (calcium metalloenzyme) \uff0c\u5b8c\u5168\u4e0d\u80fd\u5728\u6c92\u6709\u9223 (\u00a0calcium) \u7684\u60c5\u6cc1\u4e0b\u8d77\u4f5c\u7528\u3002\u5728\u4eba\u9ad4\u751f\u7406\u5b78\u4e2d\uff0c\u553e\u6db2 (\u00a0salivary) \u548c\u80f0\u6fb1\u7c89\u9176 (\u00a0pancreatic amylases) \u90fd\u662f\u4e3b\u8981\u7684\u6d88\u5316\u9176\u3002\u4eba\u985e\u589e\u52a0\u7684\u9176\u6c34\u5e73\u8207\u553e\u6db2\u5275\u50b7\u6709\u95dc;\u816e\u817a\u708e\u7531\u65bc\u553e\u6db2\u817a\u7684\u708e\u75c7\uff0c\u80f0\u817a\u708e (\u00a0pancreatitis) \u548c\u814e\u8870\u7aed (renal failure) \u3002 BioVision\u7684\u03b1-\u6fb1\u7c89\u9176\u6e2c\u5b9a\u4f7f\u7528\u4e9e\u4e59\u57fa-PNP-G7 (ethylidene-pNP-G7) \u4f5c\u70ba\u5e95\u7269\u3002\u4e00\u65e6\u5e95\u7269\u88ab\u03b1-\u6fb1\u7c89\u9176\u7279\u7570\u6027\u5207\u5272\uff0c\u7522\u751f\u7684\u8f03\u5c0f\u7684\u7247\u6bb5\u53ef\u4ee5\u88ab\u03b1-\u8461\u7cd6\u82f7\u9176 (\u03b1-glucosidase) \u4f5c\u7528\uff0c\u5c0e\u81f4\u9aee\u8272\u5718 (\u00a0chromophore ) \u7684\u6700\u7d42\u91cb\u653e\uff0c\u53ef\u65bc405nm\u6e2c\u91cf\u3002\u8a72\u6aa2\u6e2c\u6cd5\u53ef\u6aa2\u6e2c\u4f4e\u81f30.2 mU\u7684\u03b1-\u6fb1\u7c89\u9176 (\u03b1-amylase) \u542b\u91cf\u3002<\/p>\n

\"k711\"<\/a><\/p>\n

\"\"<\/p>\n

\"\u540c\u8cea\u5546\u54c17\"<\/a><\/p>\n\n\n\n\n\n\n\n\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
SKU+Size<\/strong><\/td>\nK711-100<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (405 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe assay can detect \u03b1-amylase content as low as 0.2 mU.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ less than 40 minutes
\n\u2022 Fast and convenient<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u70ba\u4e86\u80fd\u5920\u8a08\u7b97\u6a23\u54c1\u4e2d\u7684\u6fb1\u7c89\u9176\u6d3b\u6027\uff0c\u5fc5\u9808\u7372\u5f97std\u66f2\u7dda\u7684\u7dda\u6027\u7bc4\u570d\u5167\u7684\u503c\u3002 std\u7684\u6700\u9ad8\u6578\u64da\u9ede\u662f\u6bcf\u5b5420 nmol\u785d\u57fa\u915a\u542b\u91cf\u3002 \u55ae\u4f4d\u5b9a\u7fa9\uff1a\u4e00\u500b\u55ae\u4f4d\u7684\u6fb1\u7c89\u9176\u662f\u5207\u5272\u4e9e\u4e59\u57fa-pNP-G7 (ethylidene-pNP-G7) \u7684\u6fb1\u7c89\u9176\u7684\u91cf\u5728pH 7.20,25\u2103\u4e0b\u6bcf\u5206\u9418\u7522\u751f1.0\u03bcmol\u785d\u57fa\u82ef\u915a\u3002std\u66f2\u7dda\u7684\u7dda\u6027\u7bc4\u570d\u5167\u7684\u9176\u6d3b\u6027\u7684\u6700\u5927\u91cf\u70ba\u6bcf\u5b5420mU\u3002Calculation: Plot the Nitrophenol standard curve. Apply the \u2206OD (\u2206OD = ODT1 -ODT0) to the Nitrophenol standard curve to get B nmol of Nitrophenol generated by amylase between T0 and T1.<\/p>\n

\"smart\"<\/a>\"Spectrumlab<\/a><\/p>\n

\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Amylase\u6d3b\u6027\u5448\u8272\u5206\u6790\u5957\u7d44 | \u8ca8\u865fK711-100\u00a0(alpha-amylase activity)\u00a0\u00a0 […]<\/p>\n","protected":false},"author":3,"featured_media":8690,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[87,1],"tags":[3851],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9405"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9405"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9405\/revisions"}],"predecessor-version":[{"id":35613,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9405\/revisions\/35613"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8690"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9405"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9405"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9405"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}