{"id":9145,"date":"2017-03-03T11:24:30","date_gmt":"2017-03-03T03:24:30","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9145"},"modified":"2022-07-22T15:17:25","modified_gmt":"2022-07-22T07:17:25","slug":"ecori-clearcut-%e9%99%90%e5%88%b6%e9%85%b6%e9%85%b5%e7%b4%a0-%e8%b2%a8%e8%99%9fe048","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9145","title":{"rendered":"EcoRI ClearCut \u9650\u5236\u9176\u9175\u7d20 | \u8ca8\u865fE048"},"content":{"rendered":"

\u76f8\u95dc\u5546\u54c1<\/strong>\u00a0<\/span>\u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a> \u00a0| Agarose |\u00a0\u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a>\u00a0|\u00a0\u5fae\u751f\u7269\u6297\u751f\u7d20<\/a> \u00a0|\u00a0 \u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a> \u00a0|\u00a0 CMOS\u87a2\u5149\u76f8\u6a5f<\/a> \u00a0| Mupid 2 DNA\u6c34\u5e73\u96fb\u6cf3\u69fd<\/a><\/span><\/p>\n

EcoRI ClearCut <\/strong>\u00a0| <\/strong>\u8ca8\u865f<\/strong>E048<\/strong><\/a><\/span><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> EcoRI<\/strong>\u662f\u4e00\u7a2e\u6838\u9178\u9176\uff0c\u4f86\u81ea\u67d0\u4e9b\u7279\u5b9a\u54c1\u7cfb\u7684\u5927\u8178\u687f\u83cc\uff08E. coli\uff09\uff0c\u53c3\u8207\u9650\u5236\u4fee\u98fe\u7cfb\u7d71\u7684\u9175\u7d20\u3002\u5728\u5206\u5b50\u751f\u7269\u5b78\u6216\u5176\u4ed6\u5206\u5b50\u5c64\u6b21\u7684\u751f\u7269\u5b78\u7814\u7a76\u4e0a\uff0cEcoRI\u5e38\u7528<\/strong>\u65bc<\/strong>\u7279\u5b9a\u5e8f\u5217\u7684DNA\u5207\u5272\u6210\u7247\u6bb5\uff0c\u5207\u5272\u5f8c\u7684\u5c0f\u7247\u6bb5\u7aef\u9ede\u70ba5’\u7aef\u7a81\u51fa\u7684\u9ecf\u72c0\u672b\u7aef\uff08sticky end\uff09\u3002<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u5305\u88dd<\/strong><\/td>\n\n

EcoRI \u9175\u7d2020,000U\/ml<\/p>\n

10X Universal \u9650\u5236\u9176\u9175\u7d20\u53cd\u61c9\u7de9\u885d\u6db2 (Cat.No. E204)<\/td>\n<\/tr>\n

\u7522\u54c1\u61c9\u7528<\/strong><\/td>\n\u5c08\u4e00\u6027\u5207\u5272\u96d9\u80a1DNA\uff0c\u5207\u5272\u5f8c\u7684\u5c0f\u7247\u6bb5\u7aef\u9ede\u70ba5’\u7aef\u7a81\u51fa\u7684\u9ecf\u72c0\u672b\u7aef\uff08sticky end\uff09\u3002\u9175\u7d20\u5177\u6709\u9ad8\u6548\u80fd\u6d3b\u6027\uff0c\u53ea\u97005-15\u5206\u5207\u5272\u6642\u9593\u3002\u6d3b\u6027\u55ae\u4f4d\u5b9a\u7fa9\uff1a\u572837\u2103\uff0c50\u5fae\u5347\u53cd\u61c9\u9ad4\u7cfb\u4e2d\u53cd\u61c91\u5c0f\u6642\uff0c\u5c071\u5fae\u514b\u7684\u03bbDNA\u5b8c\u5168\u5206\u89e3\u7684\u9176\u91cf\u5b9a\u7fa9\u70ba1\u500b\u6d3b\u6027\u55ae\u4f4d\uff0c\u53731U\u3002<\/td>\n<\/tr>\n
\u53cd\u61c9\u6642\u9593<\/strong><\/td>\n1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)
\nIncubate at 37\u00b0C<\/td>\n<\/tr>\n
\u9175\u7d20\u4f86\u6e90\u53ca\u5207\u5272\u4f4d\u7f6e<\/strong><\/td>\nAn E. coli<\/em> strain that carries the modified EcoRI gene from E. coli<\/em> RY13. The EcoRI enzyme recognizes and cuts the sequence G^AATTC.<\/td>\n<\/tr>\n
\u9175\u7d20\u8fa8\u8996<\/strong>DNA<\/strong>\u4f4d\u7f6e<\/strong><\/td>\n\"\"<\/td>\n<\/tr>\n
Image<\/strong><\/td>\n\"\"<\/td>\n<\/tr>\n
\u8caf\u5b58\u72c0\u614b<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
\u8caf\u5b58\u7de9\u885d\u6db2<\/strong><\/td>\n20 mM Tris-HCl (pH 7.5), 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 50% (v\/v) Glycerol<\/td>\n<\/tr>\n
\u53cd\u61c9<\/strong>Buffer<\/strong><\/td>\nBuffer 1: 10%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%
\nClick here to view the buffer compatibility chart<\/a> for restriction enzymes.<\/td>\n<\/tr>\n
\u9175\u7d20\u71b1\u4e0d\u6d3b\u6027<\/strong>Heat Inactivation<\/strong><\/td>\n65\u00b0C for 20 min<\/td>\n<\/tr>\n
\u6ce8\u610f\u4e8b\u9805<\/strong><\/td>\n\n

ClearCut Enzymes are engineered to have high specificity (reduced star activity) and high activity (cuts in 5-15 minutes).<\/p>\n

Methylation Sensitivity
\ndam methylation: Not Sensitive
\ndcm methylation: Not Sensitive
\nCpG Methylation: Blocked by Some Combinations of Overlapping<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u76f8\u95dc\u5546\u54c1\u00a0\u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1 \u00a0| Agarose |\u00a0\u5fae\u751f\u7269\u57f9\u990a\u57fa\u00a0|\u00a0\u5fae\u751f\u7269\u6297\u751f\u7d20 \u00a0|\u00a0 \u8cea\u9ad4DNA\u8403\u53d6\u5957 […]<\/p>\n","protected":false},"author":3,"featured_media":9146,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[133],"tags":[3900],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9145"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9145"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9145\/revisions"}],"predecessor-version":[{"id":10058,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9145\/revisions\/10058"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/9146"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9145"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9145"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9145"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}