{"id":9140,"date":"2017-03-02T17:40:19","date_gmt":"2017-03-02T09:40:19","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9140"},"modified":"2025-03-21T15:02:51","modified_gmt":"2025-03-21T07:02:51","slug":"%e5%b0%8f%e9%bc%a0%e4%b8%bb%e5%8b%95%e8%84%88%e5%85%a7%e7%9a%ae%e7%b4%b0%e8%83%9e-mouse-primary-aortic-endothelial-cells%e8%b2%a8%e8%99%9ft4592","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9140","title":{"rendered":"\u5c0f\u9f20\u4e3b\u52d5\u8108\u5167\u76ae\u7d30\u80de | Mouse Primary Aortic Endothelial Cells\u8ca8\u865fT4592"},"content":{"rendered":"

A<\/strong>bmgood <\/strong>\u521d\u4ee3\u7d30\u80de\u7cfb\u5217\u5546\u54c1<\/strong> (Primary cell)<\/strong> |<\/strong>\u8ca8\u865f<\/strong>\u00a0\u00a0\u00a0 T4592 1×106 cells \/ 1.0 ml<\/strong><\/p>\n

Mouse Primary Aortic Endothelial Cells<\/strong><\/p>\n

\u5c0f\u9f20\u4e3b\u52d5\u8108\u5167\u76ae\u7d30\u80de <\/strong>(<\/strong>\u8ca8\u865f<\/strong>T4592<\/strong>)<\/strong><\/p>\n

Mouse Primary Aortic Endothelial Cells<\/strong>\u7d30\u80deAdherent \/ Polygonal | \u4f86\u6e90Mus musculus Aorta | \u7d30\u80de\u57f9\u990a\u689d\u4ef6: 5,000-10,000 cells\/cm2 | \u7d30\u80de\u6a19\u8a8cvWF, CD31\u3002\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>) \u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)\u3002\u7d30\u80de\u57f9\u990a\u57fa\u70baPrigrow X series medium(\u00a0TM4592<\/a>.) Atmosphere: air, 95%; Carbon dioxide (CO2), 5%.<\/p>\n

\"\"<\/p>\n

HOT!!<\/strong>\u00a0<\/u>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/p>\n

\"\u672a\u547d\u540d-14\"\u00a0<\/strong>Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/p>\n

Abmgood<\/strong>\u4e0d\u6764\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0<\/strong>(immortalized reagenet)<\/strong>\u00a0Ready-to-use |\u00a0hTERT Cell Immortalization Reagents<\/span><\/p>\n

\u5546\u54c1<\/strong>:<\/strong> Lenti-hTERT virus\u00a0<\/span><\/strong>\u75c5\u6bd2\u6db2<\/strong>(106<\/sup> IU\/ml)<\/strong>\u8ca8\u865f<\/strong>G200 |<\/strong>\u5305\u88dd<\/strong>10ml<\/strong><\/p>\n

Lentivirus containing hTERT ; Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u6764\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u6764 (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u6747\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n

\"\"<\/p>\n","protected":false},"excerpt":{"rendered":"

Abmgood \u521d\u4ee3\u7d30\u80de\u7cfb\u5217\u5546\u54c1 (Primary cell) |\u8ca8\u865f\u00a0\u00a0\u00a0 T4592 1×106 […]<\/p>\n","protected":false},"author":3,"featured_media":8019,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[494],"tags":[3901],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9140"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9140"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9140\/revisions"}],"predecessor-version":[{"id":50859,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9140\/revisions\/50859"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8019"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9140"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9140"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9140"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}