{"id":9064,"date":"2017-02-24T12:17:02","date_gmt":"2017-02-24T04:17:02","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9064"},"modified":"2023-12-12T11:06:55","modified_gmt":"2023-12-12T03:06:55","slug":"%e7%be%a5%e8%84%af%e6%b0%a8%e9%85%b8%e5%91%88%e8%89%b2%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-hydroxyproline-colorimetric-assay-kit%e8%b2%a8%e8%99%9fk555","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=9064","title":{"rendered":"\u7fa5\u812f\u6c28\u9178\u5448\u8272\u5206\u6790\u5957\u7d44 | Hydroxyproline Colorimetric Assay Kit\u8ca8\u865fK555 (abcam ab222941)"},"content":{"rendered":"

\u5546\u54c1:<\/strong> Hydroxyproline Colorimetric Assay Kit<\/strong>\u7fa5\u812f\u6c28\u9178\u5448\u8272\u5206\u6790\u5957\u7d44\u00a0(abcam ab222941)<\/a><\/span><\/p>\n

\u7522\u54c1\u898f\u683c: <\/span><\/strong>\u7fa5\u812f\u6c28\u9178\u5448\u8272\u5206\u6790\u5957\u7d44 \u8ca8\u865fK555 | 100\u500b\u5206\u6790<\/p>\n

\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"Hydroxyproline (4-hydroxyproline) \u7fa5\u812f\u6c28\u9178\uff084-\u7fa5\u57fa\u812f\u6c28\u9178\uff09\u662f\u5e38\u898b\u7684\u975e\u86cb\u767d\u751f\u6210\u6c28\u57fa\u9178\u3002\u5b83\u50c5\u5b58\u5728\u65bc\u54fa\u4e73\u52d5\u7269\u7684\u81a0\u539f\u548c\u5f48\u6027\u86cb\u767d\u4e2d\uff0c\u4f46\u5b58\u5728\u65bc\u690d\u7269\u4e2d\u7684\u8a31\u591a\u5176\u5b83\u86cb\u767d\u8cea\u4e2d\u3002\u7fa5\u812f\u6c28\u9178\u50c5\u5b58\u5728\u7ffb\u8b6f\u5f8c\u4fee\u98fe\u80bd\u93c8\u4e2d\uff0c\u812f\u6c28\u9178\u7fa5\u5316\u9176\u975e\u6e38\u96e2\u7684\u7fa5\u57fa\u5316\u812f\u6c28\u9178\u3002\u7d44\u7e54\u6c34\u89e3\u7522\u7269\u4e2d\u7684Hydroxyproline\u7fa5\u812f\u6c28\u9178\uff0c\u70ba\u6e2c\u91cfcollagen\u81a0\u539f\u6216gelatin\u660e\u81a0\u91cf\u7684\u6307\u6a19\u3002\u591a\u7a2e\u75be\u75c5\u72c0\u614b\u5f71\u97ff\u81a0\u539f\u86cb\u767d\u8f49\u8b8a\uff0c\u4e26\u53ef\u5c0e\u81f4\u8840\u6e05\u6216\u5c3f\u7fa5\u812f\u6c28\u9178\u5347\u9ad8\u3002\u9019\u7a2e\u75c5\u75c7\u7684\u7bc4\u570d\u5f9e\u816b\u7624\u3001\u708e\u75c7\u3001\u814e\u6216\u9aa8\u75be\u75c5\u5230\u5167\u5206\u6ccc\u548c\u81ea\u8eab\u514d\u75ab\u6027\u75be\u75c5\u3002 BioVision\u7684\u7fa5\u812f\u6c28\u9178\u6e2c\u5b9a\u8a66\u5291\u76d2K555-100\uff0c\u8a2d\u8a08\u7528\u65bc\u6e2c\u91cf\u7d44\u7e54\u6216\u86cb\u767d\/\u80bd\u6c34\u89e3\u7522\u7269\u4e2d\u7684\u7fa5\u812f\u6c28\u9178\u3002\u5b83\u53ef\u7528\u65bc\u6e2c\u91cf\u4f86\u81ea\u5176\u4ed6\u751f\u7269\u6a23\u54c1\u5982\u8840\u6e05\u6216\u5c3f\u6db2\u7684\u7fa5\u812f\u6c28\u9178\uff08\u5982\u679c\u5b83\u5011\u5df2\u7d93\u7d93\u6b77\u4e86\u4e4b\u524d\u7684\u7d14\u5316\u904e\u7a0b\uff09;\u65b9\u6cd5\u5bb9\u6613\u3001\u65b9\u4fbf\uff0c\u5728560nm\u8655\u5177\u6709\u6700\u5927\u5438\u5149\u5ea6\u7684\u8272\u539f\u9ad4\uff0c\u8a72\u6e2c\u5b9a\u7bc4\u570d0.1-2\u03bcg\u3002<\/p>\n

\"\"<\/p>\n

\"\u540c\u8cea\u5546\u54c17\"<\/a><\/p>\n

\"k555\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
SKU+Size<\/strong><\/td>\nK555-100<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (560 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe assay is useful over the range of 0.1-2 \u03bcg.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes less than ~100 minutes
\n\u2022 Convenient and easy to use<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Oxidation Buffer
\n\u2022 Chloramine T Concentrate
\n\u2022 Perchloric acid\/Isopropanol Solution
\n\u2022 DMAB Concentrate (in DMSO)
\n\u2022 Hydroxyproline Standard (1 mg\/ml)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u00e5\u00bc\u0095\u00e7\u0094\u00a8\u00e6\u0096\u0087\u00e7\u008d\u00bb\"<\/p>\n

\n
1. Cui, Huachun et al. (2017) miR-34a promotes fibrosis in aged lungs by inducing alveolarepithelial dysfunctions,\u00a0Am J Physiol Lung Cell Mol Physiol. 2017 Mar 1;312(3):L415-L424.<\/a><\/div>\n<\/div>\n
\n
2. Higuchi, Sei et al. (2016) EP4 Receptor\u2013Associated Protein in Macrophages Protects against Bleomycin-Induced Pulmonary Inflammation in Mice,\u00a0J Immunol. 2016 Dec 1;197(11):4436-4443.<\/a><\/div>\n<\/div>\n
\n
3. Al-Eisa, Einas S. et al. (2016) Correlation between vitamin D levels and muscle fatigue risk factors based on physical activity in healthy older adults,\u00a0Clin Interv Aging. 2016 May 4;11:513-22.<\/a><\/div>\n<\/div>\n
\n
4. Khong, Nicholas M.H. et al. (2016) Nutritional composition and total collagen content of three commercially important edible jellyfish,\u00a0Food Chem. 2016 Apr 1;196:953-60.<\/a><\/div>\n<\/div>\n
\n
5. Chagnon et al., Smart imaging of acute lung injury: exploration of myeloperoxidase activity using in vivo endoscopic confocal fluorescence microscopy.\u00a0Am J Physiol Lung Cell Mol Physiol,\u00a0Sep 2015; 309: L543 – L551.<\/a><\/div>\n<\/div>\n

\u7522\u54c1\u554f\u8207\u7b54<\/strong>\u5c3f\u6db2\u6a23\u54c1\u7684\u7d14\u5316<\/strong><\/span><\/p>\n

100\u03bcl\u8840\u6e05\/\u5c3f\u4e2d\uff0c\u52a0\u5165100\u03bcl\u6fc3\u9e7d\u9178\u3002 HCl\u5728\u58d3\u529b\u5bc6\u5c01\u7684\u7279\u6c1f\u9686\u84cb\u74f6\u4e2d\u4e26\u5728120\u2103\u6c34\u89e33\u5c0f\u6642\u3002\u52a0\u51655mg\u6d3b\u6027\u70adNorit\u6216\u6d3b\u6027\u70adDarco\uff08Sigma Cat\uff0397876\u6216242276\uff09\u6df7\u5408\u3002\u4ee512000g\u96e2\u5fc32\u5206\u9418\u3002\u5c0710ul\u4e0a\u6e05\u6db2\u8f49\u79fb\u81f396\u5b54\u677f\u4e2d\uff0c\u5728\u771f\u7a7a\u4e0b\u84b8\u767c\u81f3\u4e7e\u3002\u7e7c\u7e8c\u64cd\u4f5c\u624b\u518a\u6b65\u9a5f3\u3002<\/p>\n

\u7522\u54c1\u554f\u8207\u7b54\u6a23\u54c1\u8655\u7406\u8207\u4fdd\u5b58<\/span><\/strong><\/p>\n

\u6a23\u54c1\u7528\u9178\u6d88\u5316\/\u6c34\u89e3\u4ee5\u5f9e\u81a0\u539f\u8a08\u7b97\u7fa5\u812f\u6c28\u9178\u3002\u6a19\u6e96\u54c1\u662f\u7fa5\u4e19\u57fa\u7532\u57fa\u7e96\u7dad\u7d20\uff0c\u56e0\u6b64\u4e0d\u9700\u8981HCL \/\u84b8\u716e\u8655\u7406\u3002\u6b64\u5916\uff0c\u6a19\u6e96\u54c1\u4e0d\u662f\u771f\u7a7a\u4e7e\u71e5\u7684\uff0c\u56e0\u70ba\u5176\u4e0d\u542bHCl-\u5728\u9032\u884c\u5176\u5b83\u8a66\u5291\u4e4b\u524d\uff0c\u5c07\u6a23\u54c1\u4e7e\u71e5\u4ee5\u9664\u53bbHCl\u3002\u624b\u518a\u4e2d\uff0c\u8981\u5728\u771f\u7a7a\u4e0b\u4e7e\u71e5\u677f\u3002\u82e5\u6c92\u9019\u65b9\u9762\u8a2d\u5099\uff0c\u53ef\u4ee5\u572860\u2103\u7684\u70e4\u7bb1\u4e2d\u4e7e\u71e5\u677f\uff0c\u78ba\u4fdd\u64fa\u812bHCl\u3002\u7a7a\u6c23\u4e7e\u71e5\u9700\u8981\u5f88\u9577\u6642\u9593\uff0c\u4e0d\u63a8\u85a6\u3002\u6301\u6c34\u89e3\u7684\u88c2\u89e3\u7269\u6216\u4e7e\u71e5\u768496\u5b54\u677f\u5728-80\u651d\u6c0f\u5ea6\uff0c\u4f9b\u4ee5\u5f8c\u4f7f\u7528\u3002\u8acb\u5c07\u88c2\u89e3\u7269\u7b49\u5206\uff0c\u4e26\u5728\u5132\u5b58\u524d\u5bc6\u5c01\u677f\u3002\u6a23\u54c1\u88fd\u5099\u90e8\u5206\u4e2d\u7684\u53cd\u61c9\u6642\u9593\uff0c120.C\u9032\u884c\u5c11\u65bc3\u5c0f\u6642\uff0c\u5426\u5247\u6c34\u89e3\u5c07\u4e0d\u80fd\u6709\u6548\u5b8c\u6210\u3002\u53ef\u4ee5\u5c07\u6c34\u89e3O \/ N\u7e7c\u7e8c\u7b2c\u4e8c\u5929\uff0c\u6216\u6c34\u89e33\u5c0f\u6642\u4e26\u51cd\u7d50\u6c34\u89e3sup \u65bc-80\u2103\uff0c\u4ee5\u5099\u4f7f\u7528\u3002\u7d30\u80de\u82e5\u71d2\u7126\u7684\u5916\u89c0\u4e0d\u5f71\u97ff\u5be6\u9a57\u3002\u9810\u671f\u7576\u7d30\u80de\/\u7d44\u7e54\u53d7\u5230\u9ad8\u6eab\u8655\u74063\u5c0f\u6642\u6642\uff0c\u5b83\u5011\u6703\u7522\u751f\u71d2\u7126\u7684\u5916\u89c0\u3002\u5728\u9032\u884c\u4e0b\u4e00\u6b65\u4e4b\u524d\uff0c\u8b93\u9019\u7a2e\u9ed1\u8272\u7269\u8cea\u4f5c\u70ba\u788e\u5c51\u6c89\u6fb1\uff08\u5982\u679c\u9700\u8981\uff0c\u7d66\u7ba1\u5b50\u8f15\u8f15\u65cb\u8f49\uff09\u3002\u7136\u5f8c\u50c5\u53d6\u4e0a\u6e05\u6db2\u7528\u65bc\u4e0b\u4e00\u6b65\u84b8\u767c\u3002<\/p>\n

\u8a66\u5291\u76d2\u53ef\u4ee5\u7528\u65bc\u50cf\u7d30\u83cc\uff0c\u690d\u7269\uff0c\u679c\u8805\uff0c\u9175\u6bcd\u7b49\u6a23\u54c1\uff1bK555\u7fa5\u812f\u6c28\u9178\u5448\u8272\u5206\u6790\u5957\u7d44\uff0c\u512a\u5316\u8207\u54fa\u4e73\u52d5\u7269\u6a23\u54c1\u7684\u8a66\u5291\u76d2\u3002\u7136\u800c\uff0c\u7406\u8ad6\u4e0a\u9019\u4e9b\u8a66\u5291\u76d2\u61c9\u8207\u4f86\u81ea\u591a\u500b\u7269\u7a2e\/\u4f86\u6e90\u7684\u6a23\u54c1\u4e00\u8d77\u4f7f\u7528\u3002\u7531\u65bc\u6700\u4f73\u689d\u4ef6\u53d6\u6c7a\u65bc\u6a23\u54c1\u985e\u578b\uff0c\u56e0\u6b64\u5fc5\u9808\u8abf\u6574\u65b9\u6848\u4ee5\u9069\u61c9\u6a23\u54c1\u4ee5\u7372\u5f97\u6709\u6548\u7684\u7d50\u679c\u3002\u65b0\u9bae\u6a23\u54c1\u7e3d\u662f\u512a\u65bc\u51b7\u51cd\u6a23\u54c1\u3002\u7136\u800c\uff0c\u4e5f\u53ef\u4ee5\u4f7f\u7528\u51b7\u51cd\u6a23\u54c1\uff0c\u689d\u4ef6\u662f\u5b83\u5011\u5728\u5206\u96e2\u5f8c\u7acb\u5373\u51b7\u51cd\uff0c\u4e0d\u662f\u591a\u6b21\u51b7\u51cd\u89e3\u51cd\uff08\u70ba\u6b64\u6211\u5011\u5efa\u8b70\u5728\u51b7\u51cd\u524d, \u5206\u88dd\u591a\u7ba1\u4f7f\u7528)\u3002<\/p>\n

\"smart\"<\/a>\"Spectrumlab<\/a>\"102-T\"<\/a>\"0100-01\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u5546\u54c1: Hydroxyproline Colorimetric Assay Kit\u7fa5\u812f\u6c28\u9178\u5448\u8272\u5206\u6790\u5957\u7d44\u00a0(ab […]<\/p>\n","protected":false},"author":3,"featured_media":8690,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5380,87],"tags":[288,6428,6427,6429],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9064"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9064"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9064\/revisions"}],"predecessor-version":[{"id":47789,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9064\/revisions\/47789"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8690"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9064"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9064"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9064"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}