{"id":8958,"date":"2017-02-08T10:47:30","date_gmt":"2017-02-08T02:47:30","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8958"},"modified":"2022-07-22T15:57:35","modified_gmt":"2022-07-22T07:57:35","slug":"8958","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=8958","title":{"rendered":"CometAssay\u00ae \u5f57\u661f\u5206\u6790\u96fb\u6cf3\u8a2d\u5099 | \u8ca8\u865f4250-050-ES"},"content":{"rendered":"<p><strong>\u7522\u54c1\u4ecb\u7d39: CometAssay\u00ae \u5f57\u661f\u5206\u6790\u96fb\u6cf3\u8a2d\u5099 <\/strong><strong>\u8ca8\u865fTREVIGEN <\/strong><strong>\u00a04250-050-ES<\/strong><\/p>\n<p style=\"text-align: justify;\"><strong>\u7522\u54c1\u63cf\u8ff0:<\/strong> CometAssay\u00aeES II\u5f57\u661f\u5206\u6790\u96fb\u6cf3\u8a2d\u5099\uff0c\u4f7f\u7814\u7a76\u4eba\u54e1\u80fd\u5920\u512a\u5316\u9e7c\u6027\u548c\u4e2d\u6027\u5f57\u661f\u5206\u6790\u6e2c\u5b9a\uff0c\u7372\u5f97\u9ad8\u5ea6\u53ef\u91cd\u8907\u7684\u7d50\u679c\uff1b\u4f7f\u7528\u5f57\u661f\u5206\u6790\u5c08\u7528\u96fb\u6cf3\u8a2d\u5099\uff0c\u514b\u670d\u5f57\u661f\u6e2c\u5b9a\u7d50\u679c\u53ef\u4ee5\u6839\u64da\u6eab\u5ea6\uff0c\u96fb\u6975\u4e4b\u9593\u7684\u8ddd\u96e2\u548c\u7de9\u885d\u9ad8\u5ea6\u800c\u8b8a\u5316\u4e4b\u60c5\u6cc1\u3002\u4e26\u6a19\u6e96\u5316\u500b\u9ad4\u7528\u6236\u548c\u5be6\u9a57\u5ba4\u4e4b\u9593\u7684\u96fb\u6cf3\u65b9\u6cd5\u3002\u4f7f\u7528\u51b7\u537b\u548c\u4e00\u9ad4\u5f0f\u6a21\u88fd\u5851\u6599\u9ad4\u4fdd\u6301\u6046\u5b9a\u7684\u7de9\u885d\u6eab\u5ea6\u3001\u6700\u4f73\u7de9\u6c96\u6c34\u5e73\uff0c\u4ee5\u53ca\u91cd\u758a\u7372\u5f97\u4e00\u81f4\u7684\u7d50\u679c\u3002\u8a2d\u8a08\u7684\u6258\u76e4\u5bb9\u7d0d2\u300120\u548c96\u5b54\u73bb\u7247\uff0c\u4e26\u5728\u96fb\u6cf3\u671f\u9593\u4fdd\u6301\u6b63\u78ba\u7684\u4f4d\u7f6e\u3002\u512a\u5316\u7528\u65bcCometAssay\u00ae\u8a66\u5291\u76d2\u548cCometAssay\u00ae\u5c0d\u7167\u7d30\u80de\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"wp-image-4257\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/10\/Comet_electrophoresis_unit.jpg\" alt=\"Comet_electrophoresis_unit\" width=\"411\" height=\"270\" \/><\/p>\n<p><strong>\u5f57\u661f\u5206\u6790\u7684\u6aa2\u6e2c\u539f\u7406: <\/strong>\u662f\u57fa\u65bc\u8b8a\u6027\u7684\u539f\u7406\uff0c\u5f85\u6e2c\u7d30\u80de\u88ab\u88c2\u89e3\u8655\u7406\u4ee5\u4f7fDNA\u89e3\u65cb\u548c\u8b8a\u6027\uff0c\u4e4b\u5f8c\u7d30\u80de\u6838\u9032\u884c\u96fb\u6cf3\uff0c\u4f7f\u5f97\u7d30\u80de\u5728\u5f57\u661f\u73bb\u7247\u4e0a\u5e73\u92ea\u7684\u4f4e\u7194\u9ede\u74ca\u8102\u7cd6\u4e0a\u79fb\u52d5\uff0c\u4e4b\u5f8c\u4f7f\u7528\u8207DNA\u76f8\u4e92\u4f5c\u7528\u7684\u87a2\u5149\u67d3\u6599\u6216\u9280\u67d3\u8a66\u5291\u9032\u884c \u67d3\u8272\u4f7f\u7d50\u679c\u53ef\u8996\u3002\u5f62\u6210\u7684DNA\u5f57\u661f\u5c3e\u5f62\u72c0\u548c\u9077\u79fb\u6a21\u5f0f\uff0c\u5305\u62ec\u9e7c\u6027\u5f57\u661f\u5c3e\u9577\u5ea6\uff0c\u5c3e\u90e8DNA\u767e\u5206\u6bd4\u4ee5\u53ca\u5c3e\u77e9\u7684\u8a08\u7b97\u7b49\u8cc7\u6599\u53ef\u4f7f\u7528\u5716\u50cf\u5206\u6790\u8edf\u9ad4\u9032\u884c\uff0c\u6700\u7d42\u7528\u65bc\u8a55\u4f30\u7d30\u80de\u4e2dDNA\u7684\u640d\u50b7\u3002Trevigen\u5b8c\u6574\u7684\u5f57\u661f\u5206\u6790\u5be6\u9a57\u65b9\u6848\uff0c\u5305\u62ec\u5f57\u661f\u5206\u6790\u6aa2\u6e2c\u8a66\u5291\u76d2\u3001\u5f57\u661f\u5206\u6790\u967d\u6027\u5c0d\u7167\u7d30\u80de\u3001\u5f57\u661f\u5206\u6790\u8f09\u7247\u3001\u5f57\u661f\u5206\u6790\u6240\u9700\u7684\u5206\u6790\u548c\u67d3\u8272\u8a66\u5291\u4ee5\u53ca\u5f57\u661f\u5206\u6790\u96fb\u6cf3\u7cfb\u7d71\uff0c\u4ee5\u53ca\u5c08\u5229\u7684FLARE\u2122\u6aa2\u6e2c\u8a66\u5291\u76d2\u3002\u53ef\u6eff\u8db3\u60a8\u5f57\u661f\u5206\u6790\u7684\u6240\u6709\u9700\u8981\u3002<\/p>\n<p><strong>\u5f57\u661f\u5206\u6790<\/strong><strong>\u4e4b\u61c9\u7528<\/strong> \u5f57\u661f\u5206\u6790\u53c8\u7a31\u55ae\u7d30\u80de\u51dd\u81a0\u96fb\u6cf3\u5206\u6790\uff0c\u70ba\u6aa2\u6e2c\u7d30\u80de\u5167DNA\u640d\u50b7\u63d0\u4f9b\u7c21\u4fbf\u6709\u6548\u7684\u65b9\u6cd5\u3002\u81ea\u6210\u529f\u5730\u7528\u65bc\u6aa2\u6e2cDNA\u55ae\u93c8\u65b7\u88c2\u4ee5\u4f86\uff0c\u7d93\u4e0d\u65b7\u5730\u6539\u9032\uff0c\u5df2\u6210\u70ba\u4e00\u7a2e\u5feb \u901f\u3001\u9748\u654f\u548c\u7c21\u4fbf\u5730\u6aa2\u6e2cDNA\u640d\u50b7\u5730\u65b9\u6cd5\uff0c\u5ee3\u6cdb\u5730\u61c9\u7528\u65bcDNA\u653e\u5c04\u640d\u50b7\u3001DNA\u526a\u5207\u640d\u50b7\u3001DNA\u4ea4\u806f\u7684\u6aa2\u6e2c\u3001\u85e5\u7269\u7684\u907a\u50b3\u6bd2\u6027\u8a55\u50f9\u548c\u7d30\u80de\u51cb\u4ea1\u9452\u5b9a\u7b49\u5de5\u4f5c\u4e2d\u3002\u666e\u901a\u7684\u5f57\u661f\u5206\u6790\u53ca\u87a2\u5149\u67d3\u8272\u7522\u751f\u7684\u8cc7\u6599\u7d50\u679c\u9700\u8981\u87a2\u5149\u986f\u5fae\u93e1\u9032\u884c\u89c0\u5bdf\uff0c\u4e26\u4e14\u4e0d\u6613\u4fdd\u5b58\uff0c\u5982\u679c\u80fd\u7372\u5f97\u6c38\u4e45\u7684\u5f57\u661f\u5206\u6790\u5716\u50cf\u7d50\u679c\uff0c\u4e26\u53ef\u5728\u666e\u901a\u986f\u5fae\u93e1\u4e0b\u6aa2\u6e2c\u5247\u53ef\u4ee5\u63d0\u9ad8\u5f57\u661f\u5206\u6790\u7684\u4fbf\u6377\u6027\u3002<\/p>\n<p><strong>\u64cd\u4f5c\u6d41\u7a0b\uff1a<\/strong><\/p>\n<p style=\"padding-left: 30px;\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-661\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/COMET_procedure.png\" alt=\"comet_procedure\" width=\"571\" height=\"174\" \/><\/p>\n<ol>\n<ol>\n<li>\u5f85\u6e2c\u7d30\u80de\u8207\u4f4e\u7194\u9ede\u74ca\u8102\u81a0 \uff08LM Agarose\uff09\uff0c\u572837<sup>o<\/sup>C\u4e0b\u5747\u52fb\u6df7\u5408\u3002<\/li>\n<li>\u5c07\u7d30\u80de\u653e\u7f6e\u5728CometSlide\u2122\u4e0a\u3002<\/li>\n<li>\u7d66\u4e88\u7d30\u80deLysis Solution\uff0c\u79fb\u9664\u7d30\u80de\u819c\u8207DNA\u4e0a\u7684histones\u3002<\/li>\n<li>\u52a0\u5165\u8a66\u5291\u4f7fDNA\u8b8a\u6027\u3001\u89e3\u65cb\u3002<\/li>\n<li>\u6a23\u54c1\u4f7f\u7528\u6838\u9178\u67d3\u5291\u9032\u884c\u67d3\u8272\uff0c\u4e26\u4f7f\u7528\u87a2\u5149\u986f\u5fae\u93e1\uff0c\u89c0\u6e2c\u96fb\u6cf3\u5f8c\u65b7\u88c2DNA\u79fb\u52d5\u7684\u60c5\u5f62\u3002<span style=\"line-height: 1.5em;\">\u00a0<\/span><\/li>\n<\/ol>\n<\/ol>\n<table style=\"height: 360px;\" width=\"765\">\n<tbody>\n<tr>\n<td width=\"139\"><strong>\u5546\u54c1\u578b\u865f<\/strong><\/td>\n<td width=\"427\"><strong>\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n<td width=\"114\"><strong>\u5305\u88dd\u898f\u683c<\/strong><\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" width=\"680\"><strong>Comet Assay<\/strong><strong>\u5206\u6790\u8a66\u5291\u7d44<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"139\">4250-050-K<\/td>\n<td width=\"427\">CometAssay\u00ae Kit\uff0825X2 well slides\uff09<\/td>\n<td width=\"114\">50 samples<\/td>\n<\/tr>\n<tr>\n<td width=\"139\">4252-040-K<\/td>\n<td width=\"427\">CometAssay\u00ae Kit\uff082X20 well slides\uff09<\/td>\n<td width=\"114\">40 samples<\/td>\n<\/tr>\n<tr>\n<td width=\"139\">4253-096-K<\/td>\n<td width=\"427\">CometAssay\u00ae Kit\uff081X96 well slide\uff09<\/td>\n<td width=\"114\">96 samples<\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" width=\"680\"><strong>Comet Assay<\/strong><strong>\u5c08\u7528\u73bb\u7247<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"139\">4250-050-03<br \/>\n4250-200-03<\/td>\n<td width=\"427\">CometSlides\u2122 \u5c08\u7528\u73bb\u7247\uff082 Well\uff09<\/td>\n<td width=\"114\">25 \u7247<br \/>\n100 \u7247<\/td>\n<\/tr>\n<tr>\n<td width=\"139\">4252-040-01<br \/>\n4252-200-01<br \/>\n4252-500-01<br \/>\n4252-02K-01<\/td>\n<td width=\"427\">CometAssay\u00ae HT \u5c08\u7528\u73bb\u7247\uff0820 Well\uff09<\/td>\n<td width=\"114\">2 \u7247<br \/>\n10 \u7247<br \/>\n25 \u7247<br \/>\n100 \u7247<\/td>\n<\/tr>\n<tr>\n<td width=\"139\">4253-096-03<\/td>\n<td width=\"427\">CometSlide\u2122 \u5c08\u7528\u73bb\u7247\uff0896 Well\uff09<\/td>\n<td width=\"114\">1 \u7247<\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" width=\"680\"><strong>Comet Assay <\/strong><strong>\u5c08\u7528\u8a66\u5291<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"139\">4250-010-01<\/td>\n<td width=\"427\">CometAssay Lysis Solution<\/td>\n<td width=\"114\">100 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"139\">4250-050-02<\/td>\n<td width=\"427\">CometAssay\u00ae LM Agarose<\/td>\n<td width=\"114\">15 ml<\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" width=\"680\"><strong>Comet Assay <\/strong><strong>\u96fb\u6cf3\u8a2d\u5099<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"139\">4250-050-ES<\/td>\n<td width=\"427\">CometAssay\u00ae Electrophoresis System II<\/td>\n<td width=\"114\">1\u7d44<\/td>\n<\/tr>\n<tr>\n<td width=\"139\">4250-050-ESK<\/td>\n<td width=\"427\">CometAssay\u00ae 2 Well ES Unit w\/ Starter Kit<\/td>\n<td width=\"114\">1\u7d44\u542b\u5957\u7d44<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"color: #3366ff;\"><span style=\"color: #ff0000;\">\u66f4\u591a\u76f8\u95dc\u5546\u54c1<\/span> <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?news=safeview-%E6%96%B0%E5%9E%8B%E6%A0%B8%E9%85%B8%E5%AE%89%E5%85%A8%E6%9F%93%E5%8A%91\">\u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a> | Agarose | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=2671\">\u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?cat=451\">\u5fae\u751f\u7269\u6297\u751f\u7d20<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/www.tprobio.com\/?t-pro-plasmid-kit,48\">\u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?cat=100\">CMOS\u87a2\u5149\u76f8\u6a5f<\/a> |<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6196\">Mupid 2 DNA\u6c34\u5e73\u96fb\u6cf3\u69fd<\/a>|<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7522\u54c1\u4ecb\u7d39: CometAssay\u00ae \u5f57\u661f\u5206\u6790\u96fb\u6cf3\u8a2d\u5099 \u8ca8\u865fTREVIGEN \u00a04250-050-ES \u7522\u54c1\u63cf\u8ff0 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":8961,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[515,183],"tags":[3945],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8958"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8958"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8958\/revisions"}],"predecessor-version":[{"id":9914,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8958\/revisions\/9914"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8961"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8958"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8958"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8958"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}