{"id":8750,"date":"2017-01-17T16:52:21","date_gmt":"2017-01-17T08:52:21","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8750"},"modified":"2022-07-22T16:02:31","modified_gmt":"2022-07-22T08:02:31","slug":"%e6%96%b0%e4%b8%80%e4%bb%a3ldh%e4%b9%b3%e9%85%b8%e8%84%ab%e6%b0%ab%e9%85%b6%e5%91%88%e8%89%b2%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84ii-ldh-cytotoxicity-colorimetric-assay-kit-%e8%b2%a8%e8%99%9fk313-50","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=8750","title":{"rendered":"\u65b0\u4e00\u4ee3LDH\u4e73\u9178\u812b\u6c2b\u9176\u5448\u8272\u5206\u6790\u5957\u7d44II | LDH-Cytotoxicity Colorimetric Assay Kit \u8ca8\u865fK313-500"},"content":{"rendered":"

\u65b0\u4e00\u4ee3<\/strong>LDH<\/strong>\u4e73\u9178\u812b\u6c2b\u9176\u5448\u8272\u5206\u6790\u5957\u7d44<\/strong>II LDH-Cytotoxicity Colorimetric Assay Kit<\/strong><\/a><\/span><\/p>\n

\u7522\u54c1\u898f\u683c: LDH\u4e73\u9178\u812b\u6c2b\u9176\u5448\u8272\u5206\u6790\u5957\u7d44 \u8ca8\u865fK313-500 \u00a0| 500\u500b\u5206\u6790<\/p>\n

\u7522\u54c1\u63cf\u8ff0: \u7d93\u7531\u7d30\u80de\u819c\u7834\u640d\uff0c\u53ef\u8a55\u4f30\u7d30\u80de\u662f\u5426\u6b7b\u4ea1 (Cell death)\u6216\u7522\u751f\u7d30\u80de\u6bd2\u6027 (cytotoxicity)\u53cd\u61c9\uff1b\u7576\u7d30\u80de\u819c\u7834\u640d\u6642\uff0c\u539f\u672c\u5b58\u5728\u65bc\u7d30\u80de\u8cea\u5167\u7684\u4e73\u9178\u812b\u6c2b\u8122 (lactate dehydrogenase, LDH) \u5373\u6703\u88ab\u91cb\u653e\u81f3\u7d30\u80de\u57f9\u990a\u6db2\u4e2d\u3002\u85c9\u7531\u52a0\u5165\u53ef\u8207 LDH \u53cd\u61c9\u5f62\u6210\u6c34\u6eb6\u6027\u7d05\u8272\u7d50\u6676\u7269\u8cea\u7684\u5448\u8272\u8a66\u5291\uff0c\u5373\u53ef\u6e2c\u5f97\u7d30\u80de\u7684\u6b7b\u4ea1\u7387\u3002LDH\u4e73\u9178\u812b\u6c2b\u9176 (Lactate Dehydrogenase, LDH) \u591a\u5b58\u65bc\u7d44\u7e54\u5668\u5b98\u5982\u809d\u81df\u3001\u5fc3\u81df\u3001\u814e\u81df\u3001\u808c\u8089\u53ca\u7d05\u8840\u7403\u4e2d\u3002\u82e5\u6578\u503c\u504f\u9ad8\uff0c\u8868\u793a\u53ef\u80fd\u60a3\u6709\u5fc3\u808c\u6897\u585e\u3001\u80ba\u6813\u585e\u3001\u809d\u81df\u640d\u50b7\u3001\u808c\u8089\u767c\u80b2\u4e0d\u826f\u3001\u767d\u8840\u75c5\u3001\u8ca7\u8840\u6216\u764c\u75c7\u7b49\u3002<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u539f\u7406: Biovision\u63d0\u4f9bLDH\u5206\u6790\u5957\u7d44\uff0c\u907f\u95a7\u653e\u5c04\u7dda\u5206\u6790\u65b9\u6cd5\uff0c\u4ee5\u5448\u8272Absorbance (450 nm)\u5075\u6e2c\uff1b\u53ef\u7528\u65bc\u8cbc\u9644\u578b\u6216\u61f8\u6d6e\u578b\u7d30\u80de\u57f9\u990a\u6db2\u3002\u64cd\u4f5c\u6642\u9593\u5927\u7d0430-60\u5206\u9418\u3002\u5075\u6e2c\u539f\u7406\u70ba\u4f7f\u7528\u9176\u5076\u806f\u53cd\u61c9\u7684\u6e2c\u5b9a\uff1aLDH\u6c27\u5316\u4e73\u9178\u4ee5\u7522\u751fNADH\uff0c\u7136\u5f8c\u5176\u8207WST\u53cd\u61c9\u4ee5\u7522\u751f\u9ec3\u8272\u3002\u6240\u7522\u751f\u7684\u984f\u8272\u7684\u5f37\u5ea6\u76f4\u63a5\u8207\u88c2\u89e3\u7684\u7d30\u80de\u6578\u76f8\u95dc\u3002WST\u53cd\u61c9\u66f4\u4eae\uff0c\u6e2c\u5b9a\u50c5\u9700\u66f4\u5c11\u91cf\u7684\u57f9\u990a\u57fa\uff0c\u56e0\u6b64\u4f86\u81ea\u8840\u6e05\u548c\u57f9\u990a\u57fa\u7684\u80cc\u666f\u986f\u8457\u964d\u4f4e\u3002\u4f7f\u7528\u8a72\u6e2c\u5b9a\uff0c\u7d30\u80de\u53ef\u4ee5\u5728\u5e38\u898f\u7684\u542b\u670910\uff05\u8840\u6e05\u7684\u57f9\u990a\u57fa\u4e2d\u57f9\u990a\uff0c\u6e2c\u5b9a\u4e0d\u9700\u8981\u9084\u539f\u6027\u8840\u6e05\u6216\u7279\u6b8a\u57f9\u990a\u57fa\u3002\u6b64\u5916\uff0c\u7531\u65bcWST\u66f4\u7a69\u5b9a\uff0c\u53cd\u61c9\u53ef\u4ee5\u8b80\u53d6\u591a\u6b21\uff0c\u4e26\u4e14\u9084\u53ef\u4ee5\u5728\u53cd\u61c9\u671f\u9593\u7684\u4efb\u4f55\u6642\u9593\u9ede\u505c\u6b62 LDH\u6d3b\u6027\u53ef\u4ee5\u5bb9\u6613\u5730\u901a\u904e\u5206\u5149\u5149\u5ea6\u8a08\u6216\u677f\u8b80\u6578\u5668\u5728OD450nm\u5b9a\u91cf\u3002\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u6240\u6709\u5fc5\u9700\u7684\u8a66\u5291\uff0c\u5305\u62ecLDH\u967d\u6027\u5c0d\u7167\u7d44\u3002<\/p>\n

K313\u8207K311\u4e4b\u5957\u7d44: \u9019\u5169\u7a2e\u6aa2\u6e2c\u8a66\u5291\u76d2\u4e4b\u9593\u6709\u8a31\u591a\u4e0d\u540c\u4e4b\u8655\u3002\u6aa2\u6e2c\u6ce2\u9577-K311\uff1a500nm\uff0cK313\uff1a450nm\u539f\u7406-K311\uff1aLDH\u6d3b\u6027\u53ef\u4ee5\u901a\u904e\u5076\u806f\u7684\u9176\u4fc3\u53cd\u61c9\u4f86\u78ba\u5b9a\uff1aLDH\u5c07\u4e73\u9178\u9e7d\u6c27\u5316\u6210\u4e19\u916e\u9178\u9e7d\uff0c\u7136\u5f8c\u8207\u56db\u5511\u9e7dINT\u53cd\u61c9\u5f62\u6210formazan\u3002\u57f9\u990a\u4e0a\u6e05\u6db2\u4e2d\u7522\u751f\u7684formazan\u91cf\u7684\u589e\u52a0\u8207\u88c2\u89e3\u7d30\u80de\u6578\u91cf\u7684\u589e\u52a0\u76f4\u63a5\u76f8\u95dc\u3002 K313\uff1a\u5229\u7528\u9176\u4fc3\u5076\u806f\u53cd\u61c9\u7684\u6e2c\u5b9a\uff1aLDH\u6c27\u5316\u4e73\u9178\u7522\u751fNADH\uff0c\u7136\u5f8c\u8207WST\u53cd\u61c9\u7522\u751f\u9ec3\u8272\u3002\u7522\u751f\u7684\u984f\u8272\u7684\u5f37\u5ea6\u76f4\u63a5\u8207\u88c2\u89e3\u7684\u7d30\u80de\u6578\u91cf\u76f8\u95dc\u806f\u3002K313\u7684\u7279\u6b8a\u512a\u9ede\uff1a\u7531\u65bcWST\u8f03\u4eae\uff0c\u56e0\u6b64\u6e2c\u5b9a\u9700\u8981\u8f03\u5c11\u91cf\u7684\u57f9\u990a\u57fa\uff0c\u56e0\u6b64\u8840\u6e05\u548c\u57f9\u990a\u57fa\u7684\u80cc\u666f\u986f\u8457\u964d\u4f4e\u3002\u4f7f\u7528\u8a72\u6e2c\u5b9a\u6cd5\uff0c\u7d30\u80de\u53ef\u4ee5\u5728\u542b\u6709\u5e38\u898f10\uff05\u8840\u6e05\u7684\u57f9\u990a\u57fa\u4e2d\u57f9\u990a\uff0c\u6e2c\u5b9a\u4e2d\u4e0d\u9700\u8981\u9084\u539f\u8840\u6e05\u6216\u7279\u6b8a\u57f9\u990a\u57fa\u3002\u53e6\u5916\uff0c\u7531\u65bcWST\u66f4\u7a69\u5b9a\uff0c\u53cd\u61c9\u53ef\u4ee5\u591a\u6b21\u8b80\u53d6\uff0c\u4e5f\u53ef\u4ee5\u5728\u53cd\u61c9\u904e\u7a0b\u4e2d\u7684\u4efb\u4f55\u6642\u9593\u9ede\u505c\u6b62\u3002<\/p>\n

\u00a0<\/strong>\u66f4\u591a<\/strong>C<\/strong>ytotoxicity\u00a0 | <\/strong>\u76f8\u95dc\u5206\u6790<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u00a0K350-100<\/td>\n\u00a0Adenylate Kinase (AK) Activity Assay Kit (Colorimetric\/Fluorometric)<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K511-500<\/td>\n\u00a0Bacterial Counting Colorimetric Assay Kit\u00a0<\/a><\/td>\n\u00a0500 assays<\/td>\n<\/tr>\n
\u00a0K511-2500<\/td>\n\u00a0Bacterial Counting Colorimetric Assay Kit\u00a0<\/a><\/td>\n\u00a02500 assays<\/td>\n<\/tr>\n
\u00a0K315-100<\/td>\n\u00a0Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD\/CFSE)<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K4230-100<\/td>\n\u00a0Chloramphenicol (CAP) ELISA Kit\u00a0<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K4231-100<\/td>\n\u00a0Furazolidone ELISA Kit\u00a0<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K311-400<\/td>\n\u00a0LDH-Cytotoxicity Colorimetric Assay Kit<\/a><\/td>\n\u00a0400 assays<\/td>\n<\/tr>\n
\u00a0K313-500<\/td>\n\u00a0LDH-Cytotoxicity Colorimetric Assay Kit II<\/a><\/td>\n\u00a0500 assays<\/td>\n<\/tr>\n
\u00a0K314-500<\/td>\n\u00a0PicoProbe\u2122 LDH-Cytotoxicity Fluorometric Assay Kit<\/a><\/td>\n\u00a0500 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong>HOT<\/strong>\u76f8\u95dc\u5546\u54c1<\/strong>|<\/span>WST1<\/strong>\u7d30\u80de\u6d3b\u6027\u5206\u6790<\/strong>(<\/strong>\u5448\u8272<\/strong>)<\/strong>\u5957\u7d44<\/strong><\/a>|\u7d30\u80de\u8001\u5316\u5206\u6790\u5957\u7d44<\/strong><\/a>|\u00a0\u751f\u7269\u51b7\u5149\u7d30\u80de\u6bd2\u6027\u5206\u6790\u5957\u7d44<\/strong><\/a>\u00a0| \u00a0LDH\u7d30\u80de\u6bd2\u6027\u5206\u6790\u5957\u7d44<\/a><\/strong>|\u00a0\u6b7b\u6d3b\u7d30\u80de\u67d3\u8272<\/a>|<\/strong><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u65b0\u4e00\u4ee3LDH\u4e73\u9178\u812b\u6c2b\u9176\u5448\u8272\u5206\u6790\u5957\u7d44II LDH-Cytotoxicity Colorimetric Assay […]<\/p>\n","protected":false},"author":3,"featured_media":8363,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[123,180],"tags":[3978],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8750"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8750"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8750\/revisions"}],"predecessor-version":[{"id":32286,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8750\/revisions\/32286"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8363"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8750"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8750"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8750"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}