{"id":8607,"date":"2017-01-09T17:54:30","date_gmt":"2017-01-09T09:54:30","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8607"},"modified":"2022-07-22T16:28:52","modified_gmt":"2022-07-22T08:28:52","slug":"caspase-3cpp32%e8%9e%a2%e5%85%89%e5%8f%97%e8%b3%aa-caspase-3-substrate-devd-afc-%e8%b2%a8%e8%99%9f1007-200","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=8607","title":{"rendered":"caspase-3\/CPP32\u87a2\u5149\u53d7\u8cea | Caspase-3 Substrate DEVD-AFC \u8ca8\u865f1007-200"},"content":{"rendered":"

\u54c1\u9805<\/strong>:<\/strong> Caspase-3 Substrate DEVD-AFC | <\/strong>\u8ca8\u865f<\/strong>1007-200<\/strong><\/a><\/span><\/p>\n

\"biovision02\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>: <\/strong>\u5373\u7528\u578bDEVD-AFC\u70bacaspase-3\/CPP32 (Km = 9.7 \u00b5M)\u87a2\u5149Substrate\uff0c\u6d3b\u5316\u7684Caspase-3\u70ba\u86cb\u767d\u6c34\u89e3\u9176\uff1b\u80fd\u591a\u7279\u7570\u5207\u5272\u53d7\u8ceaAc-DEVD-AMC\u4e0a\uff0c\u65bcD(aspartate)\u548cAMC\u4e4b\u9593\uff0cAMC\u6e38\u96e2\u5f8c\u6703\u767c\u51fa\u87a2\u5149 (ex400\/em505nm)\u3002\u53ef\u4f7f\u7528\u87a2\u5149\u8a08\u6216\u591a\u76e4\u5f0f\u87a2\u5149reader\u3002 \u6216\u8005\u4f7f\u7528\u624b\u6301\u5f0f\u9577UV\u71c8\u4f7f\u87a2\u5149\u5f9e\u5207\u5272\u6642\u7684\u85cd\u8272\u5230\u7da0\u8272\u7684\u8b8a\u5316\u3002\u7522\u54c1\u898f\u683c: Ac-Asp-Glu-Val-Asp-AFC | \u7d14\u5ea6>98% by HPLC | \u6fc3\u5ea6In DMSO (1 mM) | \u5206\u5b50\u91cfC\u2083\u2080H\u2083\u2083F\u2083N\u2085O\u2081\u2083<\/p>\n

\"1007-200\"<\/a><\/p>\n

Ready-to-use fluorometric substrate for caspase-3\/CPP32 (Km = 9.7 \u00b5M) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Alternate Name<\/th>\nAc-DEVD-AFC, Caspase-3 Substrate Fluorogenic<\/td>\n<\/tr>\n
Peptide Sequence<\/th>\nAc-Asp-Glu-Val-Asp-AFC<\/td>\n<\/tr>\n
Appearance<\/th>\nLiquid<\/td>\n<\/tr>\n
Formulation<\/th>\nIn DMSO (1 mM)<\/td>\n<\/tr>\n
CAS #<\/th>\n1762-34-1<\/td>\n<\/tr>\n
Molecular Formula<\/th>\nC\u2083\u2080H\u2083\u2083F\u2083N\u2085O\u2081\u2083<\/td>\n<\/tr>\n
Molecular Weight<\/th>\n729<\/td>\n<\/tr>\n
Purity<\/th>\n>98% by HPLC<\/td>\n<\/tr>\n
Solubility<\/th>\nDMSO<\/td>\n<\/tr>\n
SMILES<\/th>\nCC(C)C(C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C(CCC(=O)O)NC(=O)C(CC(=O)O)NC(=O)C<\/td>\n<\/tr>\n
InChi<\/th>\nInChI=1S\/C30H34F3N5O13\/c1-12(2)25(38-26(47)17(6-7-21(40)41)36-28(49)18(10-22(42)43)34-13(3)39)29(50)37-19(11-23(44)45)27(48)35-14-4-5-15-16(30(31,32)33)9-24(46)51-20(15)8-14\/h4-5,8-9,12,17-19,25H,6-7,10-11H2,1-3H3,(H,34,39)(H,35,48)(H,36,49)(H,37,50)(H,38,47)(H,40,41)(H,42,43)(H,44,45)\/t17-,18-,19-,25-\/m0\/s1<\/td>\n<\/tr>\n
InChi Key<\/th>\nGZDRODOYEFEHGG-NUDCOPPTSA-N<\/td>\n<\/tr>\n
PubChem CID<\/th>\n644234<\/a><\/td>\n<\/tr>\n
MDL Number<\/th>\nMFCD01310970<\/td>\n<\/tr>\n
Handling<\/th>\nPotect from light and moisture<\/td>\n<\/tr>\n
Storage Conditions<\/th>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/th>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/th>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Caspase<\/strong>\u8a0a\u606f\u50b3\u905e\u7c21\u4ecb<\/strong><\/p>\n

\u7576\u7d30\u80de\u63a5\u6536\u51cb\u4ea1\u8a0a\u865f\u5f8c\uff0c\u5c07\u4e0d\u6d3b\u5316\u614b\u7684pro-caspase\u8f49\u8b8a\u70ba\u6d3b\u5316\u614bcaspase\uff0c\u5f15\u767ccaspase cascade\u9020\u6210\u7d30\u80de\u51cb\u4ea1\u3002\u4e3b\u8981\u7d93\u7531Caspases 8\u30019\u300112\u8abf\u63a7\u7d30\u80de\u51cb\u4ea1\u7684\u5916\u5728\u8def\u5f91(extrinsic pathway)\u3001\u5167\u5728(intrinsic pathway)\u3001\u4ee5\u53caER stress\uff0c\u6700\u5f8c\u9020\u6210Caspases 3\u7684\u6d3b\u5316\u3002Biovision\u63d0\u4f9b\u5b8c\u6574Caspase\u76f8\u95dc\u5206\u6790\u5957\u7d44\u3001inhibitor\u3001substrate\u7b49\u5404\u985e\u5546\u54c1\u3002Caspase\u00a0\u5206\u6790\u5957\u7d44\uff0fSubstrate:\u00a0<\/strong>\u5f9eCaspase1-13\u3001Pan Caspase\u7686\u80fd\u5206\u6790\u3002\u5206\u6790\u5957\u7d44\u90e8\u5206\u6709\u5316\u5b78\u5448\u8272\u53cd\u61c9\u53ca\u87a2\u5149\u53ef\u9078\u64c7\uff1bSubstrate\u90e8\u5206\u6709\u5316\u5b78\u5448\u8272\u7269\u8ceapNA\uff08\u03bb= 400 nm\uff09\u8207\u87a2\u5149\u5448\u8272\u7269\u8ceaAFC\uff08Ex\/Em\uff1d400\/505 nm\uff09\u5169\u7a2e\u53ef\u61c9\u7528\u3002Caspase Inhibitors:\u00a0<\/strong>Caspase \u6291\u5236\u5291\u4e3b\u8981\u7d50\u5408Caspase\u6d3b\u5316\u4f4d\uff0c\u9032\u800c\u9054\u5230\u8a0a\u606f\u6291\u5236\u6548\u679c\u3002fluoromethylketone\uff08FMK\uff09\u985e\u7684\u6291\u5236\u5291\uff0c\u70ba\u4e3b\u8981\u7814\u7a76\u6297\u7d30\u80de\u51cb\u4ea1\u7684\u5de5\u5177\u4e4b\u4e00\u3002\u65b0\u4e00\u4ee3Caspase\u6291\u5236\u5291Q-(XXXX)-OPh\uff0c\u56e0\u5176\u7d30\u80de\u6bd2\u6027\u8f03\u4f4e\uff0c\u4e14\u7a69\u5b9a\u6027\u8f03FMK\u985e\u6291\u5236\u5291\u9ad8\uff0c\u8f03\u9069\u5408\u5728in vivo\u5be6\u9a57\u4e2d\u4f7f\u7528\u3002<\/p>\n

\n
\"\u00e5\u00bc\u0095\u00e7\u0094\u00a8\u00e6\u0096\u0087\u00e7\u008d\u00bb\"<\/div>\n
Sin et al., Acute Treatment of Resveratrol Alleviates Doxorubicin-Induced Myotoxicity in Aged Skeletal Muscle Through SIRT1-Dependent Mechanisms. \u00a0J Gerontol A Biol Sci Med Sci,\u00a0Oct 2015; 10.1093\/gerona\/glv175.<\/a><\/div>\n<\/div>\n
\n
Pei et al., Desacyl ghrelin prevents doxorubicin-induced myocardial fibrosis and apoptosis via the GHSR-independent pathway.\u00a0Am J Physiol Endocrinol Metab, Feb 2014; 306: E311 – E323.<\/a><\/div>\n<\/div>\n
\n
Chen et al., Infection of Pericytes\u00a0In Vitro<\/em>\u00a0by Japanese Encephalitis Virus Disrupts the Integrity of the Endothelial Barrier.\u00a0J. Virol., Jan 2014; 88: 1150 – 1161.<\/a><\/div>\n<\/div>\n
Ren, K. et al. Tumor-suppressing Function of Caspase-2 Requires Catalytic Site Cys-320 and Site Ser-139 in Mice,\u00a0J. Biol. Chem., Apr 2012; 287: 14792 – 14802.<\/a><\/div>\n
\n
Rui,T. et al.,Reduction in IL-33 expression exaggerates ischaemia\/reperfusion-induced myocardial injury in mice with diabetes mellitus,\u00a0Cardiovasc Res, Feb 2012; 10.1093\/cvr\/cvs015<\/a>.<\/div>\n
<\/div>\n<\/div>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u5546\u54c1\u578b\u865f<\/td>\n\u7522\u54c1\u540d\u7a31<\/td>\n\u5305\u88dd\u898f\u683c<\/td>\n<\/tr>\n
Caspase<\/strong>\u87a2\u5149\u5206\u6790\u5957\u7d44<\/strong><\/td>\n<\/tr>\n
K110-100<\/td>\nCaspase-1 Fluorometric Assay<\/td>\n100 assays<\/td>\n<\/tr>\n
K116-100<\/td>\nCaspase-2 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K105-100<\/td>\nCaspase-3 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K126-100<\/td>\nCaspase-4 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K122-100<\/td>\nCaspase-5 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K114-100<\/td>\nCaspase-6 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K112-100<\/td>\nCaspase-8 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K118-100<\/td>\nCaspase-9 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K124-100<\/td>\nCaspase-10 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K139-100<\/td>\nCaspase-12 Fluorometric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
Caspase<\/strong>\u5448\u8272\u5206\u6790\u5957\u7d44<\/strong><\/td>\n<\/tr>\n
K111-100<\/td>\nCaspase-1 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K117-100<\/td>\nCaspase-2 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K106-100<\/td>\nCaspase-3 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K127-100<\/td>\nCaspase-4 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K123-100<\/td>\nCaspase-5 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K115-100<\/td>\nCaspase-6 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K113-100<\/td>\nCaspase-8 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K119-100<\/td>\nCaspase-9 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
K125-100<\/td>\nCaspase-10 Colorimetric Assay Kit<\/td>\n100 assays<\/td>\n<\/tr>\n
Caspase FMK<\/strong>\u985e\u6291\u5236\u5291<\/strong><\/td>\n<\/tr>\n
1012-100
\n1012-20C<\/td>\n		Caspase-1 Inhibitor, Z-YVAD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1073-100
\n1073-20C<\/td>\n		Caspase-2 Inhibitor, Z-VDVAD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1009-100
\n1009-20C<\/td>\n		Caspase-3 Inhibitor, Z-DEVD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1108-100
\n1108-20C<\/td>\n		Caspase-4 Inhibitor, Z-LEVD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1100-100
\n1100-20C<\/td>\n		Caspase-5 Inhibitor, Z-WEHD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1011-100
\n1011-20C<\/td>\n		Caspase-6 Inhibitor, Z-VEID-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1064-100
\n1064-20C<\/td>\n		Caspase-8 Inhibitor, Z-IETD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1074-100
\n1074-20C<\/td>\n		Caspase-9 Inhibitor, Z-LEHD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1112-100
\n1112-20C<\/td>\n		Caspase-10 Inhibitor, Z-AEVD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1079-100
\n1079-20C<\/td>\n		Caspase-12 Inhibitor, Z-ATAD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1115-100
\n1115-20C<\/td>\n		Caspase-13 Inhibitor, Z-LEE-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1010-100
\n1010-20C<\/td>\n		Caspase-Family Inhibitor, Z-VAD-FMK<\/td>\n100 \u03bcl\uff082 mM\uff09
\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n
1120-20C<\/td>\nCaspase Inhibitor Boc-D-FMK<\/td>\n20 \u03bcl\uff0810 mM\uff09<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u54c1\u9805: Caspase-3 Substrate DEVD-AFC | \u8ca8\u865f1007-200 \u7522\u54c1\u4ecb\u7d39: \u5373\u7528\u578b […]<\/p>\n","protected":false},"author":3,"featured_media":8500,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[128],"tags":[4012],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8607"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8607"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8607\/revisions"}],"predecessor-version":[{"id":32347,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8607\/revisions\/32347"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8500"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8607"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8607"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8607"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}