{"id":8326,"date":"2016-12-26T15:49:36","date_gmt":"2016-12-26T07:49:36","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8326"},"modified":"2025-03-21T15:03:55","modified_gmt":"2025-03-21T07:03:55","slug":"%e4%ba%ba%e9%a1%9e%e6%ad%a3%e5%b8%b8%e5%88%9d%e4%bb%a3%e5%bf%83%e8%82%8c%e7%b4%b0%e8%83%9e-human-primary-cardiomyocytes-%e8%b2%a8%e8%99%9ft4037","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=8326","title":{"rendered":"\u4eba\u985e\u6b63\u5e38\u521d\u4ee3\u5fc3\u808c\u7d30\u80de | Human Primary Cardiomyocytes \u8ca8\u865fT4037"},"content":{"rendered":"

A<\/strong>bmgood <\/strong>\u6b63\u5e38\u521d\u4ee3\u7d30\u80de\u7cfb\u5217\u5546\u54c1<\/strong> (Primary cell)<\/strong> |<\/strong>\u8ca8\u865f<\/strong>\u00a0\u00a0<\/strong>T4037 x106<\/sup> cells \/ 1.0 ml<\/strong><\/a><\/span><\/p>\n

\u4eba\u985e<\/strong>\u6b63\u5e38\u521d\u4ee3<\/strong>\u5fc3\u808c\u7d30\u80de\uff0cHuman Primary Cardiomyocytes <\/strong>\u8ca8\u865f<\/strong>T4037<\/p>\n

\"\"<\/p>\n

Human Primary Cardiomyocytes\u00a0<\/strong>\u7d30\u80deAdherent \/ Multipolar (P3\u7b2c\u4e09\u4ee3) | \u4f86\u6e90Ventricle Tissue (Homo sapiens) | T4037\u3002\u7d30\u80desarcomeric alpha-actinin, slow muscle myosin\u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a>)<\/span>\u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a>)<\/span>\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2Prigrow I\u3002\u80ce\u725b\u8840\u6e05(Cat. No.\u00a0TM999<\/a>)\u6700\u7d42\u6fc3\u5ea6\u70ba10%\u4e26\u586b\u52a01% Penicillin\/Streptomycin 1%.\u00a0. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C.<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
BioSafety Level<\/strong><\/td>\nII<\/td>\n<\/tr>\n
Organism<\/strong><\/td>\nHomo sapiens<\/td>\n<\/tr>\n
Source Organ<\/strong><\/td>\nVentricle Tissue<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nAdherent<\/td>\n<\/tr>\n
Morphology<\/strong><\/td>\nMultipolar<\/td>\n<\/tr>\n
Passage Number<\/strong><\/td>\n3<\/td>\n<\/tr>\n
Population Doubling<\/strong><\/td>\n10 PD. Doubling time: 51.5 hours<\/td>\n<\/tr>\n
Recommended Seeding Density<\/strong><\/td>\n10,000 – 15,000 cells per cm^2<\/td>\n<\/tr>\n
Markers<\/strong><\/td>\nsarcomeric alpha-actinin, slow muscle myosin<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n
Donor Age<\/strong><\/td>\n41<\/td>\n<\/tr>\n
Donor Gender<\/strong><\/td>\nFemale<\/td>\n<\/tr>\n
Donor Ethnicity<\/strong><\/td>\nCaucasian<\/td>\n<\/tr>\n
Disease<\/strong><\/td>\nNormal<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u521d\u4ee3\u7d30\u80de (Primary Cell) \u662f\u6307\u76f4\u63a5\u5f9e\u6d3b\u9ad4\u7d44\u7e54\u4e2d\u5206\u96e2\u51fa\u7684\u7d30\u80de\uff0c\u5177\u6709\u6709\u9650\u7684\u7d30\u80de\u58fd\u547d\u7121\u6cd5\u7121\u9650\u5236\u5730\u7e7c\u4ee3\u57f9\u990a\uff1b\u8207\u4e00\u822c\u5b78\u8853\u4e0a\u5e38\u7528\u7684\u7d30\u80de\u682a (Cell Line)\u4e0d\u540c\uff0c\u5177\u6709\u6709\u9650\u7684\u7d30\u80de\u58fd\u547d\u7121\u6cd5\u7121\u9650\u5236\u5730\u7e7c\u4ee3\u57f9\u990a\uff0c\u4f46\u537b\u5177\u6709\u6700\u63a5\u8fd1\u6d3b\u9ad4\u72c0\u614b\u7684\u7d30\u80de\u578b\u614b\u8207\u529f\u80fd\u7279\u6027\u3002\u5176\u5b83\u66f4\u591a\u4eba\u985e\u521d\u4ee3\u7d30\u80de\u00a0\u80f0\u817a\u661f\u72c0\u7d30\u80de\u00a0<\/u><\/a>|\u00a0\u8996\u7db2\u819c\u8272\u7d20\u4e0a\u76ae\u7d30\u80de<\/a> | \u5fc3\u808c\u7d30\u80de<\/p>\n

HOT!!<\/strong><\/span>\u00a0<\/u><\/span>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a>PriCoat\u2122 T25 Flasks | type I collagen coating | <\/a>I \u578b\u81a0\u539f(type I collagen) | Applied Cell Extracellular Matrix | <\/a>Prigrow III Medium\u521d\u4ee3\u7d30\u80de\u57f9\u990a\u6db2<\/a><\/span><\/p>\n

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Abmgood \u6b63\u5e38\u521d\u4ee3\u7d30\u80de\u7cfb\u5217\u5546\u54c1 (Primary cell) |\u8ca8\u865f\u00a0\u00a0T4037 x106 cells […]<\/p>\n","protected":false},"author":3,"featured_media":8019,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[494],"tags":[4061],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8326"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8326"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8326\/revisions"}],"predecessor-version":[{"id":50862,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8326\/revisions\/50862"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8019"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8326"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8326"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8326"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}