{"id":8320,"date":"2016-12-26T15:33:59","date_gmt":"2016-12-26T07:33:59","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8320"},"modified":"2025-03-21T15:04:18","modified_gmt":"2025-03-21T07:04:18","slug":"%e4%ba%ba%e9%a1%9e%e6%ad%a3%e5%b8%b8%e5%88%9d%e4%bb%a3%e8%a6%96%e7%b6%b2%e8%86%9c%e8%89%b2%e7%b4%a0%e4%b8%8a%e7%9a%ae%e7%b4%b0%e8%83%9e-human-primary-retinal-pigment-epithelial-cells-%e8%b2%a8","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=8320","title":{"rendered":"\u4eba\u985e\u6b63\u5e38\u521d\u4ee3\u8996\u7db2\u819c\u8272\u7d20\u4e0a\u76ae\u7d30\u80de | Human Primary Retinal Pigment Epithelial Cells \u8ca8\u865fT4057"},"content":{"rendered":"<p><strong>A<\/strong><strong>bmgood <\/strong><strong>\u6b63\u5e38\u521d\u4ee3\u7d30\u80de\u7cfb\u5217\u5546\u54c1<\/strong><strong> (Primary cell)<\/strong><strong> |<\/strong><strong>\u8ca8\u865f<\/strong> <strong>T4057<\/strong><strong> \u00a0 \u00a0\u00a0 <span class=\"abm-product-item-price-span\">5&#215;10<sup>5<\/sup> cells \/ 1.0 ml<\/span><\/strong><\/p>\n<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=8320\"><strong>\u4eba\u985e<\/strong><strong>\u6b63\u5e38\u521d\u4ee3<\/strong><strong>\u8996\u7db2\u819c\u8272\u7d20\u4e0a\u76ae\u7d30\u80de\u00a0 | Human Primary Retinal Pigment Epithelial Cells <\/strong><strong>(<\/strong><strong>\u8ca8\u865f<\/strong><strong>T4057)<\/strong><\/a><\/span><\/p>\n<p><strong>Human Primary Retinal Pigment Epithelial Cells<\/strong>\u7d30\u80deAdherent \/ Polygonal (P2\u7b2c\u4e8c\u4ee3) | \u4f86\u6e90Retina (Homo sapiens) | T4057\u3002\u7d30\u80deMarker: CK-18, CK-19, fibronectin\u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (<a href=\"http:\/\/biopioneer.com.tw\/?p=7046\">\u8ca8\u865fG299<\/a>) \u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (<a href=\"http:\/\/biopioneer.com.tw\/?p=7031\">\u8ca8\u865fG422<\/a>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2<span style=\"color: #008080;\"><a style=\"color: #008080;\" href=\"https:\/\/www.abmgood.com\/prigrow-x-series-medium-for-t4057-cell-line-tm4057.html#T4057\">Prigrow X series<\/a><\/span>\u30021%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2024\/01\/2-%E4%BA%BA%E9%A1%9E%E6%AD%A3%E5%B8%B8%E5%88%9D%E4%BB%A3%E8%A6%96%E7%B6%B2%E8%86%9C%E8%89%B2%E7%B4%A0%E4%B8%8A%E7%9A%AE%E7%B4%B0%E8%83%9E-Human-Primary-Retinal-Pigment-Epithelial-Cells-%E8%B2%A8%E8%99%9FT4057.jpg\" width=\"347\" height=\"370\" \/><\/p>\n<p>\u521d\u4ee3\u7d30\u80de (Primary Cell) \u662f\u6307\u76f4\u63a5\u5f9e\u6d3b\u9ad4\u7d44\u7e54\u4e2d\u5206\u96e2\u51fa\u7684\u7d30\u80de\uff0c\u5177\u6709\u6709\u9650\u7684\u7d30\u80de\u58fd\u547d\u7121\u6cd5\u7121\u9650\u5236\u5730\u7e7c\u4ee3\u57f9\u990a\uff1b\u8207\u4e00\u822c\u5b78\u8853\u4e0a\u5e38\u7528\u7684\u7d30\u80de\u682a (Cell Line)\u4e0d\u540c\uff0c\u5177\u6709\u6709\u9650\u7684\u7d30\u80de\u58fd\u547d\u7121\u6cd5\u7121\u9650\u5236\u5730\u7e7c\u4ee3\u57f9\u990a\uff0c\u4f46\u537b\u5177\u6709\u6700\u63a5\u8fd1\u6d3b\u9ad4\u72c0\u614b\u7684\u7d30\u80de\u578b\u614b\u8207\u529f\u80fd\u7279\u6027\u3002<span style=\"color: #ff0000;\">\u5176\u5b83\u66f4\u591a\u4eba\u985e\u521d\u4ee3\u7d30\u80de<\/span> <span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=8315\">\u80f0\u817a\u661f\u72c0\u7d30\u80de <\/a><\/span>| <span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=8320\">\u8996\u7db2\u819c\u8272\u7d20\u4e0a\u76ae\u7d30\u80de<\/a><\/span><\/p>\n<p><span style=\"color: #ff0000;\"><strong>HOT!!<\/strong><\/span><a href=\"http:\/\/biopioneer.com.tw\/?p=5545\"><span style=\"color: #ff0000;\"><u>\u00a0<\/u><\/span><span style=\"color: #3366ff;\">\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a><span style=\"color: #3366ff;\">\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5582\">DAPI\u67d3\u6838<\/a>\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5564\">\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5567\">\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5575\">\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7046\">PriCoat\u2122 T25 Flasks | type I collagen coating | <\/a><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7031\">I \u578b\u81a0\u539f(type I collagen) | Applied Cell Extracellular Matrix | <\/a><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7044\">Prigrow III Medium\u521d\u4ee3\u7d30\u80de\u57f9\u990a\u6db2\u00a0<\/a><\/span><\/p>\n<p>\u5f15\u7528\u6587\u737b<\/p>\n<ul class=\"abm-references-list\">\n<li>Shrivastava, R., Cucuat, N., Rousse, M., Weigand, T., Neto, P., Janicot, C., &amp; Shrivastava, C. &#8220;A new generation of topical chronic wound treatments containing specific MMP inhibitors&#8221; \u00a0Chronic Wound Care Management and Research \u00a01:31 (2014).<\/li>\n<\/ul>\n<table>\n<tbody>\n<tr class=\"abm-specifications-cat_no\">\n<td class=\"abm-specifications-label\" valign=\"top\">Cat. No.<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\"><span id=\"abm-specifications-cat_no\">T4057<\/span><\/td>\n<\/tr>\n<tr class=\"abm-specifications-name\">\n<td class=\"abm-specifications-label\" valign=\"top\">Name<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">Human Primary Retinal Pigment Epithelial Cells<\/td>\n<\/tr>\n<tr class=\"abm-specifications-species\">\n<td class=\"abm-specifications-label\" valign=\"top\">Organism<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">Human (H. sapiens)<\/td>\n<\/tr>\n<tr class=\"abm-specifications-tissue\">\n<td class=\"abm-specifications-label\" valign=\"top\">Tissue<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">Eye<\/td>\n<\/tr>\n<tr class=\"abm-specifications-donor_history\">\n<td class=\"abm-specifications-label\" valign=\"top\">Donor History<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">Normal tissue<\/td>\n<\/tr>\n<tr class=\"abm-specifications-growth_properties\">\n<td class=\"abm-specifications-label\" valign=\"top\">Growth Properties<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">Adherent, polygonal<\/td>\n<\/tr>\n<tr class=\"abm-specifications-cell_type\">\n<td class=\"abm-specifications-label\" valign=\"top\">Cell Type<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">Primary Cells<\/td>\n<\/tr>\n<tr class=\"abm-specifications-storage_condition\">\n<td class=\"abm-specifications-label\" valign=\"top\">Storage Condition<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">Vapor phase of liquid nitrogen, or below -130\u00b0C.<\/td>\n<\/tr>\n<tr class=\"abm-specifications-shipping_conditions\">\n<td class=\"abm-specifications-label\" valign=\"top\">Shipping Conditions<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">Ship with dry ice.<\/td>\n<\/tr>\n<tr class=\"abm-specifications-product_format\">\n<td class=\"abm-specifications-label\" valign=\"top\">Product Format<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">Frozen<\/td>\n<\/tr>\n<tr class=\"abm-specifications-intended_use\">\n<td class=\"abm-specifications-label\" valign=\"top\">Intended Use<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.<\/td>\n<\/tr>\n<tr class=\"abm-specifications-biosafety\">\n<td class=\"abm-specifications-label\" valign=\"top\">BioSafety<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">II<\/td>\n<\/tr>\n<tr class=\"abm-specifications-certificate_of_analysis\">\n<td class=\"abm-specifications-label\" valign=\"top\">Certificate of Analysis<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com.<\/td>\n<\/tr>\n<tr class=\"abm-specifications-growth_conditions\">\n<td class=\"abm-specifications-label\" valign=\"top\">Growth Conditions<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">Use of PriCoat\u2122 T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM4057) + 1% Penicillin\/Streptomycin Solution (G255), 37.0\u00b0C, 5% CO\u2082.<\/td>\n<\/tr>\n<tr class=\"abm-specifications-unpacking_storage_instructions\">\n<td class=\"abm-specifications-label\" valign=\"top\">Unpacking and Storage Instructions<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">\n<p>1. Visually examine the packaging containers for signs of leakage or breakage.<\/p>\n<p>2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130\u00b0C, preferably in liquid nitrogen vapor phase storage, until ready for use.<\/p>\n<p>To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130\u00b0C or in liquid nitrogen vapor phase. Do not store at -70\u00b0C, as it will result in loss of viability.<\/td>\n<\/tr>\n<tr class=\"abm-specifications-thawing_protocol\">\n<td class=\"abm-specifications-label\" valign=\"top\">Thawing Protocol<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">\n<p>1. Thaw cells quickly in a 37\u00b0C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.<\/p>\n<p>2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.<\/p>\n<p>3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.<\/p>\n<p>4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.<\/p>\n<p>5. Incubate the cells at the recommended conditions.<\/td>\n<\/tr>\n<tr class=\"abm-specifications-subculture_protocol\">\n<td class=\"abm-specifications-label\" valign=\"top\">Subculture Protocol<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">\n<p>Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent.<\/p>\n<p>1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.<\/p>\n<p>2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37\u00b0C, for several minutes to facilitate detachment.<\/p>\n<p>3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.<\/p>\n<p>4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.<\/p>\n<p>5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.<\/p>\n<p>6. Incubate the cells at the recommended conditions.<\/td>\n<\/tr>\n<tr class=\"abm-specifications-cryopreservation\">\n<td class=\"abm-specifications-label\" valign=\"top\">Cryopreservation<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.<\/td>\n<\/tr>\n<tr class=\"abm-specifications-material_citation\">\n<td class=\"abm-specifications-label\" valign=\"top\">Material Citation<\/td>\n<td class=\"abm-specifications-value\" valign=\"top\">If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T4057.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Abmgood \u6b63\u5e38\u521d\u4ee3\u7d30\u80de\u7cfb\u5217\u5546\u54c1 (Primary cell) |\u8ca8\u865f T4057 \u00a0 \u00a0\u00a0 5&#038;#215 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":8321,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5373,494],"tags":[4062],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8320"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8320"}],"version-history":[{"count":8,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8320\/revisions"}],"predecessor-version":[{"id":50863,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8320\/revisions\/50863"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8321"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8320"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8320"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8320"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}