{"id":8301,"date":"2016-12-26T13:09:05","date_gmt":"2016-12-26T05:09:05","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8301"},"modified":"2025-03-21T15:05:48","modified_gmt":"2025-03-21T07:05:48","slug":"%e5%b0%8f%e9%bc%a0%e5%88%9d%e4%bb%a3%e8%82%ba%e5%8b%95%e8%84%88%e5%85%a7%e7%9a%ae%e7%b4%b0%e8%83%9e-mouse-primary-pulmonary-artery-endothelial-cells-%e8%b2%a8%e8%99%9ft4510","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=8301","title":{"rendered":"\u5c0f\u9f20\u521d\u4ee3\u80ba\u52d5\u8108\u5167\u76ae\u7d30\u80de |Mouse Primary Pulmonary Artery Endothelial Cells \u8ca8\u865fT4510"},"content":{"rendered":"<p><strong>A<\/strong><strong>bmgood <\/strong><strong>\u521d\u4ee3\u7d30\u80de\u7cfb\u5217\u5546\u54c1<\/strong><strong> (Primary cell)<\/strong><strong> |<\/strong><strong>\u8ca8\u865f<\/strong><strong>\u00a0\u00a0\u00a0 T4510 x10<sup>6<\/sup> cells \/ 1.0 ml<\/strong><\/p>\n<p><strong>\u5c0f\u9f20<\/strong><strong>\u521d\u4ee3<\/strong><strong>\u80ba\u52d5\u8108\u5167\u76ae\u7d30\u80de\uff0cMouse Primary Pulmonary Artery Endothelial Cells<\/strong><strong>\u00a0<\/strong><strong>(<\/strong><strong>\u8ca8\u865f<\/strong><strong>T4510)<\/strong><\/p>\n<p><strong>Mouse Primary Pulmonary Artery Endothelial Cells<\/strong>\u7d30\u80deAdherent \/ Polygonal (P2\u7b2c\u4e8c\u4ee3) | \u4f86\u6e90Pulmonary Artery | \u5efa\u8b70\u57f9\u990a\u5bc6\u5ea65,000-10,000 cells\/cm<sup>2<\/sup> \u3002Marker: vWF, CD31\u3002\u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (<a href=\"http:\/\/biopioneer.com.tw\/?p=7046\">\u8ca8\u865fG299<\/a>) \u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (<a href=\"http:\/\/biopioneer.com.tw\/?p=7031\">\u8ca8\u865fG422<\/a>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2Prigrow X series\u30021%. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0\u00b0C.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abmgood.com\/PrimaryCells\/images\/cell-culture-overview.jpg\" alt=\"\" width=\"583\" height=\"290\" \/><\/p>\n<p>\u521d\u4ee3\u7d30\u80de (Primary Cell) \u662f\u6307\u76f4\u63a5\u5f9e\u6d3b\u9ad4\u7d44\u7e54\u4e2d\u5206\u96e2\u51fa\u7684\u7d30\u80de\uff0c\u5177\u6709\u6709\u9650\u7684\u7d30\u80de\u58fd\u547d\u7121\u6cd5\u7121\u9650\u5236\u5730\u7e7c\u4ee3\u57f9\u990a\uff1b\u8207\u4e00\u822c\u5b78\u8853\u4e0a\u5e38\u7528\u7684\u7d30\u80de\u682a (Cell Line)\u4e0d\u540c\uff0c\u5177\u6709\u6709\u9650\u7684\u7d30\u80de\u58fd\u547d\u7121\u6cd5\u7121\u9650\u5236\u5730\u7e7c\u4ee3\u57f9\u990a\uff0c\u4f46\u537b\u5177\u6709\u6700\u63a5\u8fd1\u6d3b\u9ad4\u72c0\u614b\u7684\u7d30\u80de\u578b\u614b\u8207\u529f\u80fd\u7279\u6027\u3002<\/p>\n<p><span style=\"color: #ff0000;\"><strong>HOT!!<\/strong><\/span><a href=\"http:\/\/biopioneer.com.tw\/?p=5545\"><span style=\"color: #ff0000;\"><u>\u00a0<\/u><\/span><span style=\"color: #3366ff;\">\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a><span style=\"color: #3366ff;\">\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5582\">DAPI\u67d3\u6838<\/a>\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5564\">\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5567\">\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0<strong>|<\/strong>\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=5575\">\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7046\">PriCoat\u2122 T25 Flasks | type I collagen coating | <\/a><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7031\">I \u578b\u81a0\u539f(type I collagen) | Applied Cell Extracellular Matrix | <\/a><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7044\">Prigrow III Medium\u521d\u4ee3\u7d30\u80de\u57f9\u990a\u6db2 <\/a><\/span><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Abmgood \u521d\u4ee3\u7d30\u80de\u7cfb\u5217\u5546\u54c1 (Primary cell) |\u8ca8\u865f\u00a0\u00a0\u00a0 T4510 x106 cells [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":8019,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[494],"tags":[4065],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8301"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8301"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8301\/revisions"}],"predecessor-version":[{"id":50866,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8301\/revisions\/50866"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8019"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8301"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8301"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8301"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}