{"id":8239,"date":"2016-12-22T13:50:20","date_gmt":"2016-12-22T05:50:20","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8239"},"modified":"2022-07-22T16:51:04","modified_gmt":"2022-07-22T08:51:04","slug":"tunel-dna%e7%89%87%e6%ae%b5%e5%81%b5%e6%b8%ac-apo-brdu-red-dna-fragmentation-assay-kit%e8%b2%a8%e8%99%9fk404-60","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=8239","title":{"rendered":"(Red) TUNEL\u87a2\u5149\u986f\u5fae\u93e1\u89c0\u5bdf | Apo-BrdU-Red\u2122 DNA Fragmentation Assay Kit\u8ca8\u865fK404-60"},"content":{"rendered":"

\u5feb\u901f<\/strong>TUNEL<\/strong>\u87a2\u5149<\/strong>F<\/strong>low<\/strong>\u5206\u6790\u53ca\u87a2\u5149\u986f\u5fae\u93e1\u5546\u54c1<\/strong> (<\/strong>\u7d05\u87a2\u5149<\/strong>)<\/strong><\/em> | <\/strong>\u8ca8\u865f<\/strong>K404-60<\/strong><\/a><\/span><\/p>\n

\u7522\u54c1\u63cf\u8ff0<\/strong>:<\/strong> Apo-BrdU-Red\u2122 DNA Fragmentation Assay Kit<\/strong>\u5546\u54c1\u8a2d\u8a08\u539f\u7406BrdU-RED\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u6aa2\u6e2c\u51cb\u4ea1\u7d30\u80de\u4e2d\u7684DNA\u93c8\u65b7\u88c2\u3002BrdU\u4ee5\u7d05\u5149\u6a19\u5b9a\uff0c\u5c08\u9580\u8a2d\u8a08\u7528\u65bc\u542bGFP\u7684\u7d30\u80de\uff0c\u4ee5\u63d0\u4f9b\u4e0d\u540c\u87a2\u5149\u4e4bTUNEL\u5206\u6790\u65b9\u6cd5\u3002 Flow cytometry (Ex\/Em = 488\/576 nm for BrdU-Red and 488\/655 nm for 7-AAD). \u5957\u7d44\u5167\u63d0\u4f9b\u96d9\u8272\u6aa2\u6e2c\uff1b\u53ef\u61c9\u7528\u6d41\u5f0f\u7d30\u80de\u8853BrdU-Red\uff08Ex \/ Em =488\/576 nm\uff0c7-AAD\u7684488\/655 nm\uff09\u6216\u87a2\u5149\u986f\u5fae\u93e1\u6aa2\u6e2c\u3002\u53ef\u61c9\u7528\u65bc\u7d44\u7e54\u5207\u7247\u6216\u56fa\u5b9a\u4e4b\u7d30\u80de\u3002\u8a66\u5291\u76d2\u5167\u63d0\u4f9b\u5b8c\u6574\u7684\u7d44\u4ef6\uff0c\u5305\u62ec\u967d\u6027\u548c\u9670\u6027\u5c0d\u7167\u7d30\u80de\uff0c\u65b9\u4fbf\u61c9\u7528\u65bc\u57f9\u990a\u7d30\u80de\u548c\u7d44\u7e54\u5207\u7247\u4e2d\uff0c\u5224\u8b80DNA\u7247\u6bb5\u3002<\/p>\n

\"\"<\/p>\n

\"\u672a\u547d\u540d-14\"\u00a0TUNEL-<\/strong>\u87a2\u5149<\/strong>F<\/strong>low<\/strong>\u53ca\u87a2\u5149\u986f\u5fae\u93e1\u5546\u54c1<\/strong> (<\/strong>\u7da0\u87a2\u5149<\/strong>)<\/strong> | <\/strong>\u8ca8\u865f<\/strong>K401-60<\/strong><\/span><\/p>\n

\u7522\u54c1\u63cf\u8ff0<\/strong>:<\/strong> Apo-BrdU DNA Fragmentation Assay Kit\u5546\u54c1\u8a2d\u8a08\u539f\u7406BrdU-FITC\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u6aa2\u6e2c\u51cb\u4ea1\u7d30\u80de\u4e2d\u7684DNA\u93c8\u65b7\u88c2\u3002BrdU\u63d0\u4f9b\u5feb\u901f\u65b9\u4fbf\u4e4bTUNEL\u5075\u6e2c\uff0c\u7c21\u55ae\u6d41\u7a0b\u53ea\u97001\u5c0f\u6642\u5b8c\u6210\u3002\u65bcFlow cytometry\u53ca\u87a2\u5149\u986f\u5fae\u93e1\u89c0\u5bdf\u4e2d\uff0c\u5176\u9748\u654f\u5ea6\u9ad8\u65bc\u751f\u7269\u7d20\u5316\u6216\u5730\u9ad8\u8f9b\u5316\u3002\u5957\u7d44\u5167\u63d0\u4f9b\u96d9\u8272\u6aa2\u6e2c\uff1b\u53ef\u61c9\u7528\u6d41\u5f0f\u7d30\u80de\u8853\uff08Ex \/ Em = FITC\u7684488 \/ 520nm\uff0cPI\u7684488 \/ 623nm\uff09\u6216\u87a2\u5149\u986f\u5fae\u93e1\uff08FITC\u548cRhodamine\uff09\u96d9\u8272\u6aa2\u6e2c\u3002\u53ef\u61c9\u7528\u65bc\u54fa\u4e73\u52d5\u7269\u4e4b\u7d30\u80de\u57f9\u990a\uff08\u7c98\u9644\u548c\u61f8\u6d6e\uff09\u6216\u77f3\u881f\u5305\u57cb\u7d44\u7e54\u5207\u7247\u3002\u8a66\u5291\u76d2\u5167\u63d0\u4f9b\u5b8c\u6574\u7684\u7d44\u4ef6\uff0c\u5305\u62ec\u967d\u6027\u548c\u9670\u6027\u5c0d\u7167\u7d30\u80de\u6216\u8f09\u73bb\u7247\uff0c\u65b9\u4fbf\u61c9\u7528\u65bc\u57f9\u990a\u7d30\u80de\u548c\u7d44\u7e54\u5207\u7247\u4e2d\uff0c\u5224\u8b80DNA\u7247\u6bb5\u3002<\/p>\n

\u7522\u54c1\u4ecb\u7d39:\u00a0<\/strong>DNA\u7247\u6bb5\u5316\u70ba\u7d30\u80de\u51cb\u4ea1\u7684\u5176\u4e2d\u4e00\u9805\u7279\u5fb5\u3002\u7d30\u80de\u51cb\u4ea1\u6d3b\u5316\u5167\u751f\u6027\u7684\u5167\u5207\u9176\uff0c\u63a5\u8457\u5728\u6838\u5167\u5c07\u67d3\u8272\u8ceaDNA\u5207\u65b7\u6210\u4ee5180 bp\u70ba\u500d\u6578\u7684\u5c0f\u7247\u6bb5\uff08360\u3001540 etc.\uff09\u3002\u9019\u500b\u73fe\u8c61\u53ef\u4ee5\u88ab\u4f8b\u5982DNA laddering assay\u3001TUNEL assay\u3001\u6216Nicoletti assay\u7528\u4f86\u5075\u6e2c\u7d30\u80de\u51cb\u4ea1\u3002TUNEL assay\uff0c\u5168\u540d\u70baTerminal deoxynucleotidyl transferase (TdT) dUTP nick end labeling assay\u3002\u7d30\u80de\u5728\u53d7\u5230\u5167\u5207\u9176\u9020\u6210\u7684DNA\u65b7\u88c2\u5f8c\uff0c\u5229\u7528TdT\u50ac\u5316\u4f7fBrdUTP\u80fd\u8207\u65b7\u88c2\u8655\u4e4b3\u2019-OH\u9032\u884c\u53cd\u61c9\uff0c\u9032\u800c\u5c07\u5e36\u6709\u87a2\u5149\u6a19\u5b9a\uff08\u5982\uff1aFITC\u7b49\uff09\u7684BrdU\u6297\u9ad4\u8207BrdUTP\u7d50\u5408\uff0c\u89c0\u5bdf\u5176\u87a2\u5149\u5206\u4f48\u3002BioVision\u63d0\u4f9b\u56db\u7a2e\u4ee5TUNEL\u70ba\u539f\u7406\u4e4b\u539f\u4f4dDNA\u7247\u6bb5\u5316\u5206\u6790\u8a66\u5291\u76d2\u3002\u6240\u6709\u8a66\u5291\u76d2\u5167\u63d0\u4f9b\u5b8c\u6574\u7684\u7d44\u4ef6\uff0c\u5305\u62ec\u967d\u6027\u548c\u9670\u6027\u5c0d\u7167\u7d30\u80de\u6216\u8f09\u73bb\u7247\uff0c\u65b9\u4fbf\u61c9\u7528\u65bc\u57f9\u990a\u7d30\u80de\u548c\u7d44\u7e54\u5207\u7247\u4e2d\uff0c\u5224\u8b80DNA\u7247\u6bb5\u3002\u53ef\u4ee5\u6d41\u5f0f\u7d30\u80de\u5100\u6216\u87a2\u5149\u986f\u5fae\u93e1\u89c0\u5bdf\u5be6\u9a57\u7d50\u679c\u3002\u00a0<\/strong><\/p>\n

\"\u672a\u547d\u540d-14\"\u00a0\u7d44\u7e54\u67d3\u8272\u5c08\u7528\u5546\u54c1<\/strong> |<\/strong>\u8ca8\u865f<\/strong>K403-50<\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0<\/strong>:<\/strong> ApoBrdU-IHC\u2122 DNA Fragmentation Assay Kit<\/strong>\uff1a<\/strong>\u4f7f\u7528\u96d9\u67d3 (DAB\u5448\u8272\/ methyl green\u67d3\u8272) \u5075\u6e2c\u7d30\u80de\u7247\u6bb5\u5316\u73fe\u8c61\uff0c antiBrdU \u6297\u9ad4\u5177\u6709Biotin\u6a19\u8a8c\uff0c\u53ef\u63d0\u9ad8\u5075\u6e2c\u4e4b\u9748\u654f\u5ea6\uff0c\u5c08\u9580\u8a2d\u8a08\u7d66\u514d\u75ab\u7d44\u7e54\u67d3\u8272\uff08IHC\uff09\u4f7f\u7528\u3002\u53ef\u61c9\u7528\u65bc\u7d44\u7e54\u5207\u7247\u6216\u56fa\u5b9a\u4e4b\u7d30\u80de\u3002\u53e6\u63d0\u4f9b\u967d\u6027\u548c\u9670\u6027\u5c0d\u7167\u73bb\u7247control slides\uff0c\u65b9\u4fbf\u61c9\u7528\u65bc\u7d30\u80de\u548c\u7d44\u7e54\u5207\u7247\u4e2d\uff0c\u5224\u8b80DNA\u7247\u6bb5\u3002<\/p>\n

\"\u672a\u547d\u540d-14\"\u00a0\u55ae\u6b65\u9a5f\u87a2\u5149<\/strong>F<\/strong>low<\/strong>\u5206\u6790<\/strong> (<\/strong>\u6d41\u6c0f\u7d30\u80de\u5c08\u7528<\/strong>)<\/strong> |<\/strong>\u8ca8\u865f<\/strong>K402-50\"New\u00a0:\u00a0CXCR4<\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0<\/strong>:<\/strong> Apo-DIRECT\u2122 DNA Fragmentation Assay Kit<\/strong>\uff0c<\/strong>\u63d0\u4f9b<\/strong>\u55ae\u6b65\u9a5f\u76f4\u63a5label\u51cb\u4ea1\u7d30\u80de\uff0c\u53ef\u61c9\u7528\u65bc\u6d41\u5f0f\u7d30\u80de\u5100\u7d50\u679c\u5206\u6790FITC-dUTP\uff08Ex \/ Em =488\/520 nm\uff0cPI=488\/655 nm\uff09\u6216\u87a2\u5149\u986f\u5fae\u93e1\uff08FITC\u548cRhodamine\u6ffe\u7247\uff09\u96d9\u8272\u6aa2\u6e2c\u3002\u53ef\u61c9\u7528\u65bc\u8cbc\u9644\u578badherent \u6216\u61f8\u6d6e\u578bsuspension\u4e4b\u7d30\u80de\u3002\u8a66\u5291\u76d2\u5167\u63d0\u4f9b\u5b8c\u6574\u7684\u7d44\u4ef6\uff0c\u5305\u62ec\u967d\u6027\u548c\u9670\u6027\u5c0d\u7167\u7d30\u80de\uff0c\u65b9\u4fbf\u61c9\u7528\u65bc\u57f9\u990a\u7d30\u80de\u548c\u7d44\u7e54\u5207\u7247\u4e2d\uff0c\u5224\u8b80DNA\u7247\u6bb5\u3002<\/p>\n\n\n\n\n\n\n\n
\u7522\u54c1\u7de8\u865f<\/strong><\/td>\n\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u5305\u88dd<\/strong><\/td>\n<\/tr>\n
\u00a0K401-60<\/td>\n\u00a0ApoBrdU DNA Fragmentation Assay Kit<\/a><\/strong><\/td>\n\u00a060 assays<\/td>\n<\/tr>\n
\u00a0K404-60<\/td>\n\u00a0ApoBrdU Red DNA Fragmentation Kit<\/a><\/strong><\/td>\n\u00a060 assays<\/td>\n<\/tr>\n
\u00a0K403-50<\/td>\n\u00a0ApoBrdU-IHC DNA Fragmentation Assay Kit<\/a><\/strong><\/td>\n\u00a050 assays<\/td>\n<\/tr>\n
\u00a0K402-50<\/td>\n\u00a0ApoDIRECT DNA Fragmentation Assay Kit<\/a><\/strong><\/td>\n\u00a050 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7d30\u80de\u51cb\u4ea1\u76f8\u95dc\u7814\u7a76<\/span> \u00a0Annexin V\u6aa2\u6e2c\u5957\u7d44 | Caspase\u6d3b\u6027\u5206\u6790\u5957\u7d44 | Caspase\u6291\u5236\u5291 | TUNEL\u7247\u6bb5\u5206\u6790\u5957\u7d44 | Caspase\u6297\u9ad4<\/span><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u5feb\u901fTUNEL\u87a2\u5149Flow\u5206\u6790\u53ca\u87a2\u5149\u986f\u5fae\u93e1\u5546\u54c1 (\u7d05\u87a2\u5149) | \u8ca8\u865fK404-60 \u7522\u54c1\u63cf\u8ff0: Apo-Brd […]<\/p>\n","protected":false},"author":3,"featured_media":1590,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[130],"tags":[4071],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8239"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8239"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8239\/revisions"}],"predecessor-version":[{"id":32453,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8239\/revisions\/32453"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/1590"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8239"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8239"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8239"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}