{"id":8224,"date":"2016-12-21T17:28:26","date_gmt":"2016-12-21T09:28:26","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8224"},"modified":"2023-02-22T15:45:13","modified_gmt":"2023-02-22T07:45:13","slug":"crispr%e5%9f%ba%e5%9b%a0%e7%b5%84%e8%a3%82%e8%a7%a3%e6%aa%a2%e6%b8%ac-crispr-genomic-cleavage-detection-kit%e8%b2%a8%e8%99%9fg932","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=8224","title":{"rendered":"CRISPR\u57fa\u56e0\u7d44\u88c2\u89e3\u6aa2\u6e2c | CRISPR Genomic Cleavage Detection Kit\u8ca8\u865fG932"},"content":{"rendered":"<p><strong><span style=\"color: #33cccc; font-size: 12pt;\"><a style=\"color: #33cccc;\" href=\"http:\/\/biopioneer.com.tw\/?p=8224\" target=\"_blank\">Genome editing\u7cfb\u5217\u5546\u54c1 | CRISPR Genomic Cleavage Detection Kit\u8ca8\u865fG932<\/a><\/span><\/strong><\/p>\n<p><span style=\"color: #ff6600;\"><strong>CRISPR<\/strong><strong>\u57fa\u56e0<\/strong>\u7de8\u8f2f<strong>\u5075\u6e2c\u5957\u7d44<\/strong><\/span><\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/abm%E5%95%86%E5%93%81%E6%8F%92%E5%9C%96-300x207.jpg\" alt=\"abm\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>Abmgood\u63d0\u4f9b\u4e4bCRISPR\u57fa\u56e0\u7d44\u88c2\u89e3\u6aa2\u6e2c\u8a66\u5291\u76d2\u88ab\u8a2d\u8a08\u70ba\u4e00\u7a2e\u7c21\u55ae\uff0c\u6709\u6548\u7684\u65b9\u6cd5\u4f86\u9a57\u8b49\u57fa\u56e0\u7d44\u7de8\u8f2f\u904e\u7a0b\u3002CRISPR\u7de8\u8f2f\u7684\u6a23\u54c1\u7528\u4f5c\u91dd\u5c0d\u7279\u5b9a\u76ee\u7684\u5340\u57df\u7684PCR\u53cd\u61c9\u4e2d\u7684\u6a21\u677f\u3002\u7136\u5f8c\u5c07\u7522\u7269denatured\u548cre-annealed\u4ee5\u5728\u96d9\u93c8\u5167\u7522\u751fmismatches\u3002\u5957\u7d44\u5167\u6aa2\u6e2c\u9176\u80fd\u5920\u8b58\u5225\u9019\u7a2emismatches\u4e26\u5207\u5272\uff0c\u5728\u51dd\u81a0\u5206\u6790\u6642\u5bb9\u6613\u5340\u5206\u7684\u689d\u5e36\u5927\u5c0f\u3002\u5373\u7528\u578bCRISPR\u57fa\u56e0\u7d44\u5207\u5272\u6aa2\u6e2c\u8a66\u5291\u76d2\u65b9\u4fbf\u5730\u5305\u542b\u6240\u6709\u5fc5\u9700\u7684\u8a66\u5291\uff0c\u5305\u62ec\u4e00\u5957\u63a7\u5236\u6a21\u677f\u548c\u5f15\u7269\uff0c\u4ee5\u78ba\u4fdd\u53ef\u9760\u7684\u7d50\u679c\u3002<\/p>\n<table class=\"bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\">\n<thead>\n<tr>\n<th><strong>PRODUCT COMPONENT<\/strong><\/th>\n<th><strong>QUANTITY<\/strong><\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td><span lang=\"EN-US\">Cell Lysis Buffer\u00a0<\/span><\/td>\n<td><span class=\"gmail-CharacterStyle1\"><span lang=\"EN-US\">1.25 ml<\/span><\/span><\/td>\n<\/tr>\n<tr>\n<td>Protein Degarder<\/td>\n<td><span class=\"gmail-CharacterStyle1\"><span lang=\"EN-US\">25 rxn (50\u00a0\u03bcl)<br \/>\n<\/span><\/span><\/td>\n<\/tr>\n<tr>\n<td><span lang=\"EN-US\">Detection Enzyme<\/span><\/td>\n<td><span class=\"gmail-CharacterStyle1\"><span lang=\"EN-US\">13 \u03bcl<\/span><\/span><\/td>\n<\/tr>\n<tr>\n<td>10X Detection Buffer<\/td>\n<td>100 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td>Control Primer &amp; Template<\/td>\n<td>10\u00a0\u03bcl<\/td>\n<\/tr>\n<tr>\n<td>MegaFi\u2122 Pro Fidelity 2X MasterMix<\/td>\n<td>1.25 ml<\/td>\n<\/tr>\n<tr>\n<td>Nuclease-Free\u00a0H<sub>2<\/sub>O<\/td>\n<td>1.0 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u7522\u54c1\u7279\u9ede<\/span><\/strong><\/span><\/p>\n<p>Precision\u662f\u57fa\u56e0\u7d44\u7de8\u8f2f( genome editing )\u548c\u57fa\u56e0\u4fee\u98fe(gene modification)\u65b9\u6cd5\u7684\u95dc\u9375\u54c1\u8cea\u3002\u78ba\u8a8d\u57fa\u56e0\u7de8\u8f2f(gene editing)\u6210\u529f\u662f\u4efb\u4f55\u8a2d\u7f6e\u4e2d\u7684\u91cd\u8981\u4e00\u6b65\uff0c\u5982\u679c\u53ca\u65e9\u767c\u73fe\u5be6\u9a57\u4e0d\u8db3\uff0c\u53ef\u4ee5\u7bc0\u7701\u6642\u9593\u548c\u8cc7\u6e90\u3002 abm \u7684 CRISPR \u57fa\u56e0\u7d44\u5207\u5272\u6aa2\u6e2c\u8a66\u5291\u76d2(CRISPR Genomic Cleavage Detection Kit, G932)\u65e8\u5728\u4f5c\u70ba\u4e00\u7a2e\u7c21\u55ae\u800c\u6709\u6548\u7684\u65b9\u5f0f\u4f86\u9a57\u8b49\u60a8\u7684\u57fa\u56e0\u7d44\u7de8\u8f2f\u904e\u7a0b\u3002CRISPR \u7de8\u8f2f\u7684\u6a23\u672c\u7528\u4f5c PCR \u53cd\u61c9\u7684\u6a21\u677f(template)\uff0c\u91dd\u5c0d\u60a8\u611f\u8208\u8da3\u7684\u7279\u5b9a\u5340\u57df\u3002\u4f7f\u7528\u8a72\u8a66\u5291\u76d2\u6aa2\u6e2c\u57fa\u56e0\u7d44\u5207\u5272(genomic cleavage)\u9700\u8981\u4e00\u5c0d\u4f4d\u65bc\u6bcf\u500b sgRNA \u9776\u4f4d\u9ede\u5169\u5074\u7684\u5f15\u7269\uff0c\u53ef\u5f9e abm\uff08\u8ca8\u865f C336 &#8211; sgRNA PCR \u5f15\u7269\u5c0d\u8a2d\u8a08\u548c\u5408\u6210\u670d\u52d9, \u00a0<a href=\"https:\/\/www.abmgood.com\/Primer-Design-Primer-Synthesis.html\">sgRNA PCR Primer Pair Design &amp; Synthesis Service<\/a>\uff09\u8a02\u8cfc\u3002\u7136\u5f8c\u5c07\u7522\u7269\u8b8a\u6027(denatured)\u4e26re-annealed\u4ee5\u5728\u96d9\u93c8\u5167\u7522\u751f\u932f\u914d\u3002\u6211\u5011\u7684\u6aa2\u6e2c\u9176\u80fd\u5920\u8b58\u5225\u6b64\u985e\u932f\u914d\u4e26\u5207\u5272\u93c8\u4ee5\u7522\u751f\u5728\u51dd\u81a0\u5206\u6790\u4e2d\u6613\u65bc\u5340\u5206\u7684\u689d\u5e36\u5927\u5c0f\u3002\u5373\u7528\u578b CRISPR \u57fa\u56e0\u7d44\u5207\u5272\u6aa2\u6e2c\u8a66\u5291\u76d2(CRISPR Genomic Cleavage Detection Kit, G932)\u65b9\u4fbf\u5730\u5305\u542b\u6240\u9700\u7684\u6240\u6709\u5fc5\u8981\u8a66\u5291\uff0c\u5305\u62ec\u4e00\u7d44\u63a7\u5236\u6a21\u677f(\u00a0control template)\u548c\u5f15\u7269(\u00a0primers)\uff0c\u4ee5\u78ba\u4fdd\u7372\u5f97\u53ef\u9760\u7684\u7d50\u679c\u3002 PCR\u64f4\u589e\u5f8c\u5c0d\u7167\u6a21\u677f\u7684\u5927\u5c0f\u7d04\u70ba750bp\uff0c\u9176\u5207\u5f8c\u7684\u5927\u5c0f\u7d04\u70ba500bp\u548c250bp\u3002\u7531\u65bc\u8655\u7406\u6642\u9593\u77ed\uff0c\u9019\u7a2e\u5373\u7528\u578b\u6aa2\u6e2c\u5c07\u6210\u70ba\u4efb\u4f55\u57fa\u56e0\u7d44\u7de8\u8f2f\u5de5\u5177\u7bb1(\u00a0Genome editing)\u7684\u91cd\u8981\u88dc\u5145\u3002\u00a0Genome editing<\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/02\/CRISPR%E5%9F%BA%E5%9B%A0%E7%B5%84%E8%A3%82%E8%A7%A3%E6%AA%A2%E6%B8%AC-CRISPR-Genomic-Cleavage-Detection-Kit%E8%B2%A8%E8%99%9FG932-1.jpg\" alt=\"\" \/><\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2021\/08\/CRISPR\u57fa\u56e0\u7d44\u88c2\u89e3\u6aa2\u6e2c-CRISPR-Genomic-Cleavage-Detection-Kit\u8ca8\u865fG932.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%8E%9F%E5%BB%A0%E6%93%8D%E4%BD%9C%E4%B8%8B%E8%BC%89-300x75.png\" alt=\"G932\" width=\"143\" height=\"37\" \/><\/a><\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/02\/CRISPR%E5%9F%BA%E5%9B%A0%E7%B5%84%E8%A3%82%E8%A7%A3%E6%AA%A2%E6%B8%AC-CRISPR-Genomic-Cleavage-Detection-Kit%E8%B2%A8%E8%99%9FG932-2.jpg\" alt=\"\" \/><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/catalog\/product\/cache\/926507dc7f93631a094422215b778fe0\/g\/9\/g932-crispr-genomic-cleavage-detection-kit_data1.jpg\" alt=\"\" \/><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/catalog\/product\/cache\/926507dc7f93631a094422215b778fe0\/g\/9\/g932-crispr-genomic-cleavage-detection-kit_data1.jpg\" alt=\"\" \/><\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>Kit Features:<\/strong><\/span><\/p>\n<ul>\n<li>Ease of use with simple steps<\/li>\n<li>Rapid set-up<\/li>\n<li>Streamlined protocol suitable for high-throughput applications<\/li>\n<\/ul>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2016\/12\/G932-300x170.png\" alt=\"G932\" width=\"297\" height=\"155\" \/><\/p>\n<p><strong><span style=\"color: #ff0000;\">\u76f8\u95dc\u5546\u54c1<\/span><\/strong><span style=\"color: #3366ff;\"> <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=8224\">CRISPR\u6aa2\u6e2c\u5957\u7d44<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6466\">CRISPR\u57fa\u56e0\u5254\u9664\u7d30\u80de\u682a<\/a> | <a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6785\">CASPASE9 (D10A) NLS<\/a><\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%BC%95%E7%94%A8%E6%96%87%E7%8D%BB-300x110.jpg\" alt=\"\u00e5\u00bc\u0095\u00e7\u0094\u00a8\u00e6\u0096\u0087\u00e7\u008d\u00bb\" width=\"97\" height=\"36\" \/><\/p>\n<ul class=\"abm-references-list\">\n<li>Chung, G\u00a0<i>et al.<\/i>\u00a0&#8220;CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans.&#8221; DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016\/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.<\/li>\n<li>Chung, G\u00a0<i>et al.<\/i>\u00a0&#8220;CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans.&#8221; DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016\/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.<\/li>\n<\/ul>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/02\/CRISPR%E5%9F%BA%E5%9B%A0%E7%B5%84%E8%A3%82%E8%A7%A3%E6%AA%A2%E6%B8%AC-CRISPR-Genomic-Cleavage-Detection-Kit%E8%B2%A8%E8%99%9FG932-4.jpg\" alt=\"\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Genome editing\u7cfb\u5217\u5546\u54c1 | CRISPR Genomic Cleavage Detection  [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":8225,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[133,88,1],"tags":[4073],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8224"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8224"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8224\/revisions"}],"predecessor-version":[{"id":38775,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8224\/revisions\/38775"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8225"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8224"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8224"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8224"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}